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Effect of biotic and abiotic inducers on the activities of chitinase and peroxidase and rust control caused by Puccinia psidii on Eucalyptus

This study was aimed at verifying the effect of biotic and abiotic resistance inducers on the activities of chitinase and peroxidase as well as on the reduction of rust caused by Puccinia psidii in eucalyptus. Thus, seedlings of two 60-day eucalyptus clones were kept in a greenhouse and treated with Bion® (Acibenzolar-S-methyl, ASM), Agro-Mos®, Saccharomyces cerevisiae, Dipel®, Ecolife 40®, and Crop-set®, at 5 days before the pathogen inoculation. A suspension of P. psidii uredospores, collected from naturally infected plants, was adjusted to 5 x 10(4) uredospores/mL. Inoculation was carried out onto the dorsal leaf surface and 15 days later the disease severity was assessed according to a grading scale. ASM, S. cerevisiae and Ecolife treatments presented the best results for disease control, and the remaining treatments were not effective. Chitinase and peroxidase activity assay only included the two first treatments (ASM and S. cerevisiae). The increase in the activity of these enzymes was observed at 48 hours after the fungus inoculation in both clones previously treated with inducers.

resistance induced; diseases; Myrtaceous rust; PR Proteins


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