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Effect of manipueira on Meloidogyne incognita through in vitro and in vivo essays on tomatoes in greenhouse

The aim of this work was to study the nematicidal effect of manipueira on the control of Meloidogyne incognita. In vitro tests were split into two completely randomized assays: 1. manipueira 100%, 75%, 50% and 25% in 12 replications; 2. manipueira 15%, 10%, 8%, 6%, 4% and 2% in 5 replications. The pot assay followed a completely randomized design with the treatments manipueira 10%, 25% and 50% in 5 replications. For both assays the positive control was water and the negative control was the nematicide Carbofuran (50 mg.L-1 of active ingredient (a.i.) or 2 g of commercial product/pot). For in vitro assays the variables assessed were live or dead nematodes and number of galls on inoculated plants. For the pot assay the variables evaluated were plant height, root length, number of galls, number of eggs and reproduction factor (RF). The in vitro results indicated that only the manipueira treatments from 100% to 10% dilution showed maximum control. The pot assay demonstrated that the treatment manipueira 50% caused better development of tomato shoots than the others. However, manipueira 10% and 25% were more effective against of M. incognita in pots.

Solanum lycopersicum; wastewater extract; cassava industry; root-knot nematode


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