Intraspecifi c variation in alkaline phosphatase activity in Phaeodactylum tricornutum ( Bacillariophyceae , Bohlin )

To describe potential intraspecifi c variation in phosphorus incorporation in two strains of Phaeodactylum tricornutum (Bohlin), Ub3 and Ub7, alkaline phosphatase (AP) activity was evaluated via enzyme-labeled fl uorescence assay. Analysis using the probe ELF-97® provides individual evaluation, and therefore can determine the nutritional status of inorganic phosphorus in phytoplanktonic cells. Bioassays compared the control treatment to both phosphateenriched and phosphate-depleted treatments by varying only the phosphate concentration in the media. Th e P. tricornutum strains exhibited diff erences in their development when incubated in the phosphate-enriched media. Th e development of the Ub7 strain diff ered by exhibiting “luxury uptake” and utilization of organic phosphorus, and the alkaline phosphatase analysis indicated limitations of this clone under such conditions. Th e Ub7 strain showed higher AP activity, when compared to Ub3, in the P-enriched condition. P. tricornutum presented increases in AP activity and low variation in Surface/Volume ratio, by increasing biovolume and its maximum linear dimension, as strategies for phosphate incorporation. Our results highlight intraspecifi c diff erences in alkaline phosphatase activity, and hence diff erences in the incorporation of organic phosphorus, as the tested species regulated enzymatic activity under diff erent external phosphate concentrations.


Introduction
Biochemical characteristics of microalgae are dependent on taxonomy as well as on individual physiological characteristics and their response to light and nutrient availability.For example, the same concentration of a nutrient could mean repletion for one species and nutritional stress for another.Internal concentrations of proteins, nucleic acids, phospholipids, phosphorus reserves and nitrogen depend on cell nutritional history and physiological and taxonomic diff erences (Falkowski 2000;Vance et al. 2003).One way to assess diff erences in nutrient incorporation within and among species is by studying the activity of a specifi c enzyme.
Phosphorus (P) is a limiting nutrient in some freshwater and coastal marine ecosystems (Gregoracci et al. 2012;Maitra et al. 2015).According to Strojsová et al. (2003) and Reynolds (2006), the enzymatic production of phosphatases, which hydrolyze dissolved organic compounds, is an important physiological trait that allows some species to endure conditions of limited phosphate.Alkaline phosphatase (AP) is an extracellular enzyme expressed by a wide variety of phytoplankton species in response to the condition of limited phosphorus.Highly stable in seawater, it is a membrane-associated enzyme that catalyzes the hydrolysis of phosphorus-containing organic compounds such as phosphate esters (Fitzgerald & Nelson 1966;Graziano et al. 1996;Dyhrman 2005;Pandey & Parveen 2011), to obtain orthophosphate, a form of inorganic phosphorus directly available to organisms (Pandey & Parveen 2011).In other words, alkaline phosphatase facilitates the use of dissolved organic phosphorus (DOP) by phytoplankton when dissolved inorganic phosphorus (DIP) is limited in the environment (Lin et al. 2011).The activity of alkaline phosphatase is related to different phosphorus concentrations (Hernández et al. 2002), with it being produced when cells are experiencing phosphate deprivation or it being suppressed in conditions of sufficient phosphate (Kuenzler & Perras 1965;Fitzgerald & Nelson 1966;Grainger 1989;Vance et al. 2003).
Many phytoplankton species have increased alkaline phosphatase (AP) activity in P-limited environments, and so its activity can be used as an indicator of phosphorus deficiency in these organisms (Rengefors et al. 2001).Previous studies have observed an increase in AP activity under conditions of depletion of inorganic P in Chlorella pyrenoidosa, Scenedesmus dimorpha, Microcystis aeruginosa and Aphanizomenon flos-aquae (Beardall et al. 2001); Alexandrium fundyense, Amphidinium sp. and Isochrysis galbana (González-Gil et al. 1998);and Phaeodactylum tricornutum (i.e. Lin et al. 2013;Yang et al. 2014).On the other hand, Marco & Orus (1988) observed that the cyanobacteria Trichodesmium sp. and Anabaena sp., under similar conditions of P limitation, showed no significant increase in the production of AP.These findings demonstrate that each species responds differently to the different environmental conditions of either sufficient or depleted nutrients (Rengefors et al. 2001).
This study evaluates the incorporation of phosphorus in two clones of Phaeodactylum tricornutum in order to describe that allow these organisms to out-compete other individuals in conditions of phosphate deprivation, and to be outcompeted in conditions of replete phosphate.

Sterilization and pre-experimental preparation
Glass vessels were cleaned by immersing them in a 10% chloridric acid bath for 24 hours, rinsed with abundant distilled water, dried in an area protected from dust and autoclaved (30 minutes, 121 o C), according to the protocols described in Kawachi & Noel (2005).Plastic vessels that could not be autoclaved were cleaned as described above and then sterilized under UV radiation (260 nm) for 10 minutes (Kawachi & Noel 2005).
The seawater used in all experimental treatments and as a control was collected with a Van Dorn bottle from coastal waters (southeastern Brazil near Ilha Grande-RJ), filtered through a 150-μm mesh to remove microzooplankton and through 0.45 and 0.22 μm cartridges (Milipore ® ), to eliminate phytoplankton and bacterioplankton.The water was then sterilized by UV radiation and autoclaved prior to use in the experiments.

Organism and culture conditions
The unicellular diatom Phaeodactylum tricornutum (Bacillariophyceae; strains Ub3 and Ub7 available at the Instituto Oceanográfico, Universidade de São Paulo Marine Microorganisms Culture Collection) used in this study was isolated from coastal waters in southeastern Brazil (Ubatuba, state of São Paulo).These non-axenic strains have been maintained in the Marine Microalgae Culture Collection of the Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro in Guillard F/2 medium (Guillard & Ryther 1962) for many generations.Prior to experimentation, cells were batch-cultured in 500 mL Erlenmeyer flasks containing 250 mL of enriched seawater (34 salinity) with F/2 medium until the end of the log growth phase (5-8 days).The cells were then washed by centrifugation (three times, 2000 x g for 10 minutes) in control seawater and remained for 12 hours in 500 ml of this water for starvation.During the experiments, cultures were exposed to 80 μE.m -2 .s - PAR provided by fluorescent lamps (Sylvania cool white tubes, 40 W), under a 12:12 h light:dark cycle and a temperature of 20º C (± 0.5° C).Cultures were manually shaken two times a day.Cell growth was followed by direct microscopic cell counts of culture subsamples, using a Sedgewick Rafter Counting Cell, after treating with acidic Lugol's solution, and by optical density.Cultures were set at an initial cell density of about 1.0 x 10 3 cells.mL - and inoculated with three treatments by the addition of silica: 1) Control -control water enriched with Guillard F/2 medium (36 μM); 2) F (Phosphate-enriched) -treatment with F/2 enriched with phosphate at medium proportion of F (72 μM); and 3) F/8 (Phosphate-depleted) -treatment with F/2 under phosphate deprivation at medium proportion of F/8 (4.5 μM) (Guillard & Ryther 1962).Bioassays were performed in triplicate within 15 days, and cell number was used to calculate the growth rate of all treatments.

Alkaline phosphatase incorporation assays
Alkaline phosphatase activity of the tested microalgae was determined using an ELF-97 endogenous phosphatase detection kit (Molecular Probes, E-6601 ® ) following the method of Gonzalez-Gil et al. (1998), as modified by Dyhrman & Palenik (2001) and Skelton et al. (2006).Aliquots of 5 mL of each culture were removed during late exponential (12 day culture) and stationary (15 day culture) Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) growth phases, fixed with 1 mL of 70% ethanol for 30 minutes and washed by centrifugation at 2000 x g for 10 minutes.The supernatant was then aspirated with the aid of a vacum pump.
Prior to use, the ELF-97 ® substrate was diluted in ELF detection buffer at a ratio of 1:20 and filtered through a 0.2 μm cartridge (Milipore ® ); 100 μL of this solution was added to each sample, which were then incubated at 20°C for 30 minutes.Following incubation, the cells were washed and centrifuged (2000 x g for 10 minutes) in 1 mL solution of 10 mM phosphate buffered saline (PBS) for five times, resuspended in 1 mL PBS and filtered (3 μm black membranes-Poretics ® ).Slides of the resulting product were prepared for observation under an epifluorescence microscope.
The microalgae were analyzed to determine the enzymatic activity of alkaline phosphatase (fluorescence of ELF-97 ® ) using a Nikon ® epifluorescence microscope (model Eclipse E200), equipped with a mercury lamp of 100W and an epifluorescence filter device with excitation at 360 ± 40 nm and emission at 535 ± 50 nm (# 31060v2; Chroma Technology Corp., Rockingham, VT).
Qualitative analysis involved counts of total cells and cells with ELFA precipitate (termed labeled cells).Images were obtained with the aid of a digital camera (Canon ® ) attached to the epifluorescence microscope.

Cell volume
Cell volume and surface area were estimated at the end of the experiment (stationary phase) by measuring 40 cells randomly chosen from each clone and each treatment, according to the formulation proposed by Sun & Liu (2003), using an inverted optical microscope.

Phosphate analysis
The phosphate concentration of each treatment was analyzed during the beginning of the experiment (time zero), and during late exponential (12 day culture) and stationary (15 day culture) growth phases, using FIA (Flow Injection Analysis -FOSS Tecator FiaStar 5000 analyzer-FOSS analytical, Hillerod, Denmark).Analyses were performed according to the specifications and recommendations of Note AP 5201 (ISO 13395: 1996-revision 2, detection limit 0.05 μmol.L -1 ).

Half-saturation constant (Ks -μM)
The clones of P. tricornutum were grown in continuous cultures (1L) in F/2 medium (Guillard & Ryther 1962).The experiment was repeated three times, and the data were considered as triplicates.Cells (10 6 cells.L -1 ) were maintained in suspension using a magnetic stirrer at 30 rpm in the culture conditions described in item 2.2.The population reached the steady state between 10 and 12 days, when cell concentration varied less than 5 %.Until reaching the steady state, cell density data was used to calculate growth rates.
Upon reaching steady state, 10 μM of phosphate (final concentration) was added to the continuous culture.After this pulse, 5 mL subsamples were taken every 15 minutes for 10 hours (time experimentally determined by Riegman et al. 2000), and filtered through a 0.2 μm membrane (Acrodisc, Gelman Sciences ® ).This experiment was designed to determine the rate of cellular uptake (Vt) at a given time (t) by the maximum rate of incorporation established immediately after the pulse of 10 μM phosphate: Where V t (which is equal to ; where is the cell concentration and S is the external nutrient concentration) is the cellular uptake rate at time t.V max is the maximum incorporation rate measured at t = 0; ΔQ is the amount of phosphate incorporated at time t, and Q max t is the maximum amount of phosphate that cells can incorporate after the pulse at a given time t.The half saturation constant (ks) was estimated by ks = (Riegman & Mur 1986).

Cell quota (Q -μM)
The phosphate ate was obtained by subtracting the final concentration of phosphorus from its initial concentration in the medium during the stationary phase, since this latter phase is with nutrient limitation and exhibited maximum cell growth.
Intracellular phosphorus ([P] int.-pg P.Cell -¹): Intracellular phosphorus was calculated by normalizing the cell quota per total cellular density in the steady state.

Statistical analysis
The results were analyzed by a Nonparametric Kruskal-Wallis ANOVA and Median Test, followed by Multiple comparisons of mean ranks for all groups, with a significance level of α = 0.05, using Statsoft Statistica 7.0 software.

Results and discussion
The growth of the Phaeodactylum tricornutum strains Ub3 and Ub7 under laboratory conditions showed a lag phase of 4 days.After this time, cells grew actively up to the stationary phase with the Ub3 clone having higher growth rates in all treatments than the Ub7 strain (Tab.1), with the exception of the F/8 treatment in which both grew at Domênica Teixeira de Lima, Fernanda Reinhardt Piedras, Flávia Previero Nogueira, Eduardo da Cunha Richard, Bianca Reis Castaldi Tocci1, Jose Juan Barrera Alba and Gleyci Aparecida Oliveira Moser similar rates and achieved similar densities (Fig. 1A-B).Th e highest densities of Ub3 were observed at the end of the exponential growth phase (10-13 days) in the control (4.1 x 10 6 ± 2.1 x 10 5 cells.mL - ) and the F (4.0 x 10 6 ± 1.4 x 10 5 cells.mL - ) treatment (Fig. 1A).During the same period, the densities of Ub7 in the F treatment and the control were similar (3.0 x 10 6 ± 7.0 x 10 4 cells.mL - ) (Fig. 1B).Although phytoplankton adjust their growth rates to changes in environmental conditions, variation in maximum cell densities may be explained by diff erences in experimental conditions, such as the phosphate concentration of the culture media (Harris 1978).
In the literature, P. tricornutum is frequently reported as an opportunistic species with fast growth (Falciatore et al. 1999;Martino et al. 2007;Desbois et al. 2010), as was observed in the present study (see growth curves in Fig. 1A-B).Th is species reaches the stationary phase from the forth to the seventh day of growth, with maximum cell density varying from 10 4 to 10 7 cells.mL - .As with growth rate, maximum cell density can vary considerably among strains of the same species under the same culture conditions (i.e.Aidar et al. 1991;Voltolina et al. 1998;Foster et al. 2008;Ohse et al. 2008;Lin et al. 2013).
Both strains of P. tricornutum had lower growth rates when subjected to P-depleted conditions.Specifi c growth rates observed in the tested strains were similar to those described by Gaeta (1985), who reported growth rates of 1.13 ± 0.06 d -1 and 1.39 ± 0.08 d -1 in phosphate-enriched media (105 μM and 210 μM), and by Lin et al. (2013), who observed rates of 0.8 d -1 in non-limited P-conditions.
Concerning biovolume, the Ub3 strain presented a lower biovolume and a surface/volume ratio (S/V) similar to that of the Ub7 strain, as was expected for an organism developing in a P-depleted medium (Tab.2).However, the highest changes were observed in the Ub7 strain, with maximum biovolume in the P-depleted treatment, despite having a S/V ratio similar to the control (Tab.2).Th is variation among treatments is probably related to the growth phase, since measurements were performed in the stationary phase with the cells limited by nutrients (Timmermans & Wagt 2010).Th e two strains of P. tricornutum adapted to low phosphorus concentrations by increasing, as observed in Ub7 strain, or decreasing, as employed by Ub3 strain, biovolume, and slightly altering S/V ratios.Th us, the Ub7 strain could better exploit the absorption of this nutrient in the depleted treatment since increased biovolume and maintenance of S/V ratio are related to absorption and intracellular phosphorus storage (luxury uptake).
Th e activity of alkaline phosphatase in the two strains of P. tricornutum exhibited diff erent responses for incorporating phosphorus into their cells under diff erent conditions of phosphate availability.Th e Ub3 strain exhibited higher enzymatic activity in the P-limited treatment (Fig. 2A-B), although the diff erence was statistically signifi cant only during the stationary growth phase (p < 0.05; Tab.3), indicating that AP activity is higher in cells under conditions of low phosphate concentrations.Th ese results agree with previous studies (González-Gil et al. 1998;Beardall et al. 2001;Dyhrman & Palenik 2001;Moser et al. 2010;Yang et al. 2014), since this microalgae is able to use a strategy of organic phosphorus incorporation when the inorganic form of this nutrient is limited (Rengefors et al. 2001;Nedoma et al. 2003).On the other hand, the control and F treatment  Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) (P-replete conditions) for Ub7 exhibited greater proportions of labeled cells in the exponential growth phase compared to the P-limited treatment (Fig. 3A), results also found for diatoms of a freshwater reservoir and hypereutrophic lakes (Strojsová et al. 2003;Cao et al. 2005).However, despite the increase in labeled cells, these treatments did not diff er signifi cantly from each other (p > 0.05; Tab.3), indicating phosphate limitation for the Ub7 strain in all tested conditions.At stationary growth phase, the enzymatic activity was similar to that observed for the Ub3 strain, with a greater number of labeled cells being found in the P-depleted treatment (p < 0.05; Tab. 3).It is noteworthy that the phosphate cell quota in the Ub7 strain, in both treatments, was lower than those of the Ub3 strain (Tab.4), which suggests increased uptake of phosphate by this algae.Intraspecifi c variation in AP activity in P. tricornutum has been previously reported, hence this microalgae is used worldwide in bioassays of algal growth potential (Yang et al. 2014 and references therein).Lin et al. (2013) observed that P. tricornutum growing in a starved medium had intense AP activity from the fourth to the seventh day of incubation, and that AP mRNA expression was abundant.Additionally, Yang et al. (2014) found two genes responsible for encoding alkaline phosphatases in P. tricornutum, which exhibit sharp rises in their transcription when limited by phosphorus.In such conditions, the increase in AP activity may be related to the incorporation of organic phosphorus.In contrast, Ruiz et al. (1997) found that P. tricornutum growing in phosphate concentrations ranging from 2.5 to 50 μM, exhibited no signifi cant diff erences in alkaline phosphatase activity.However, when the microalgae were incubated at concentrations higher than 50 μM, a large decrease in enzymatic activity was observed after four days until it ceased its production.
The activity of AP seems to be controlled by the availability of inorganic phosphorus for some, but not all, phytoplankton species, suggesting that enzyme activity is not a universal measure of phosphorus requirement (Rengefors et al. 2001).In the experiments conducted by Marco & Orus (1988), the cyanobacteria Trichodesmium sp. and Anabaena sp.exhibited no signifi cant increase in AP in limited concentrations of inorganic phosphorus, while Keenan & Auer (1974), detected no AP activity in Microcystis aeruginosa and Selenastrum capricornutum.Experiments conducted using P-starved Microcystis aeruginosa cells indicated that colonial morphotypes grow in low P levels, while unicellular morphotypes consumed more P and had higher alkaline phosphatase activity in P-depleted conditions (Shen & Song 2007).
An inverse relationship between the intracellular concentration of phosphorus, cell quota (Q) and the phosphate content of the medium is well established (Kuenzler & Ketchum 1962;Fitzgerald & Nelson 1966;Hernández et al. 1993;Ruiz et al. 1997).Kuenzler & Ketchum (1962), found that cells of P. tricornutum growing in high concentrations of phosphate (80 μM) exhibited a high cell quota after a long period of nutrient accumulation, while cells incubated under limiting conditions were capable of removing almost all of the nutrients from the media before signifi cant cell multiplication occurred.As culture growth continues, the intracellular concentration of phosphorus declined in the limited treatment (8 μM) indicating that P.  tricornutum had become phosphorus defi cient and stopped multiplying.
Th e intracellular concentration of phosphorus of the Ub7 strain observed in this study ranged from 0.6 to 4.7 pg P. Cell -1 in P-depleted (F/8) and P-replete (F) treatments, respectively (Tab.4).Nevertheless, the control for both strains showed intracellular P concentrations (~2.0 pg P. Cell -1 ) similar to those reported by Kuenzler & Ketchum (1962) when phosphate depletion limited cell growth (8μM).It should also be noted that in the present work, the highest cell quota and intracellular concentration of phosphorus were observed in the Ub7 strain in the P-replete condition (Tab.4), suggesting that this strain possesses a large capacity to store phosphorus, which has also been described for P. tricornutum by Terry et al. (1983) and diatoms and other organisms of a natural community (Domingues et al. 2011;2015).
Th e two strains of P. tricornutum exhibited diff erent phosphate half-saturation constants (Ks).Th e Ks value estimated for Ub7 was twice that of Ub3, indicating lower affi nity for phosphate, which can be further enhanced throughout growth.Th ese results indicate that under P-enriched conditions (72 μM) the Ub7 strain of P. tricornutum has a greater ability to store intracellular phosphorous.However, this phosphate pool is not incorporated into the maintenance of cellular metabolism in the same proportion that it is in Ub3, since lower cell densities were observed in all treatments (Fig. 1B).
According to Monod (1949), higher Ks values are correlated with a lower affi nity for a specifi c nutrient by microorganisms, as well as lower specifi c growth rate.Th us, when nutrient concentration is low in a culture medium, the growth rate becomes limited and dependent on this nutrient.Diff erent competitive strategies for phosphate are possible (Sommer 1984).One possibility is that species with smaller Ks values have greater affi nity for absorption of nutrients providing an advantage in nutrient limited environments.In this sense, the lowest value of Ks and higher cell densities observed in Ub3 suggest a strategy to more eff ectively exploit the availability of phosphate, especially in the limited treatment.On the other hand, species with high nutrient absorption and lower growth rates favor nutrient storage inside the cells.Th is excessive consumption ("luxury uptake"), which refers to the absorption and storage of phosphorous at levels greater than those required for immediate growth, could favor these species during periods of reduced availability of nutrients (Keenan & Auer 1974;Mackey et al. 2012; Wasmund et al.Intraspecific variation in alkaline phosphatase activity in Phaeodactylum tricornutum (Bacillariophyceae, Bohlin) 2014; Domingues et al. 2015).Th is particular uptake was shown by the Ub7 clone and evidenced by it having the highest half-saturation constant and higher intracellular concentrations of phosphorus in the P-enriched treatment.Consequently, cell division may proceed for a certain period, without subsequent nutrient uptake (Kuenzler & Ketchum 1962), since phosphate reserves can sustain growth for several generations in the absence of phosphorus in the medium (Fitzgerald & Nelson 1966).Th e prompt absorption of nutrients is a common strategy by which phytoplankton exploit the nutrient resources available in oligotrophic waters (Goldman & Glibert 1982), while the absorption rate may increase up to 100 times in cells limited by phosphorus (Brown et al. 1978).
In accordance with Aidar et al. (1991), the highest phosphate absorption rates occurred during the lag phase of cell growth, indicating that phosphate absorption occurred immediately.However, signifi cant increase in biomass only occurred late in the exponential phase.Th ese results demonstrate that the processes of absorption and assimilation are diff erent, and their indicator rates do not occur simultaneously (Goldman & Glibert 1983;Collos 1986).For many microalgae, the processing of absorbing nutrient metabolism into biomass is not instantaneous and requires a particular period of time that varies among species.For some organisms, the lag phase of cell division is so extensive that absorption and growth are not on the same time scale.Th ese diff erent scales have important implications for interpreting competition among species for limiting nutrients (Collos 1986).
Experimental results show that cells in conditions of phosphorus defi ciency can achieve higher absorption rates than cells in conditions of phosphate saturation.In those defi ciency circumstances, P. tricornutum are able to absorb from 8 to 16 times as much as the minimum cell quota, by converting phosphate into intracellular polyphosphate reserves to support three to four generations in conditions of phosphate depletion (Yao et al. 2011).Th e isolated strains of the same species may refl ect signifi cant genetic variability that is refl ected in variability of characters of cellular physiology (Gallagher 1980;1982;Huang et al. 2011;Yang et al. 2014).Th is agrees with our results, since Ub3 and Ub7 both showed diff erent responses in relation to absorption and phosphate assimilation, as shown by Domênica Teixeira de Lima, Fernanda Reinhardt Piedras, Flávia Previero Nogueira, Eduardo da Cunha Richard, Bianca Reis Castaldi Tocci1, Jose Juan Barrera Alba and Gleyci Aparecida Oliveira Moser their absorption rates (Tab.4).Additionally, Yang et al. (2014) analyzed transcriptional changes in P. tricornutum under phosphorus stress and found two genes encoding phosphatases, and 11 encoding phospholipases involved in certain key genes for adaptation to P limitation.The sequencing of the same species by Huang et al. (2011), revealed 13 novel microRNAs (miRNAs) that perform specific regulatory functions in metabolism related to the synthesis of fatty acids and the urea cycle under nitrogen and silica limitation.When restricted to low iron concentrations, P. tricornutum employed metabolic rearrangements to acclimatize to the restricted nutrient levels (Allen et al. 2008).According to Terry et al. (1983), the physiological differences between strains of P. tricornutum may be greater than those found among different species of microalgae.In Gaeta (1985), statistical differences found in physiological parameters, such as growth rate, productivity and carbon assimilation, and the polymorphism shown by the clones, endorse the existence of different physiological strains of the same species (P.tricornutum).Furthermore, Martino et al. (2007) analyzed intraspecific genetic diversity of 10 strains and found indications of four different genotypes that exhibited distinct phenotypic characteristics related to the physiological differences of the morphotypes of P. tricornutum.
Many species of microalgae are able to obtain phosphorus from phosphate esters to sustain growth in an environment restricted by orthophosphate, and different algae differ in the presence and location of phosphatases (González-Gil et al. 1998;Dyhrman & Palenik 1999;Rengefors et al. 2001).The incorporation of organic nutrients by P. tricornutum is widely known, with alkaline phosphatase activity consistently bound to the cells (Kuenzler & Perras 1965) and the plasma membrane as the site of greater specificity of PA (Flynn et al. 1986).Likewise, in this study, the greatest ELFA precipitation was observed around the cells of P. tricornutum and attached to the plasma membrane (Fig. 4B).

Conclusions
Despite Phaeodactylum tricornutum usually having greater enzymatic activity in conditions of phosphate depletion, intraspecific variation was observed.
The Ub7 strain displays deficiency in phosphate incorporation, even in P-enriched conditions when there was excessive consumption of phosphate, alkaline phosphatase activity and use of P-organic.Through the use of organic phosphorus, P. tricornutum, in exponential growth phase, is capable of storing a higher internal concentration of phosphorus, however, this storage does not reflect an increase of cell density in the stationary phase, showing a probable imbalance between the absorption and assimilation of this nutrient.
The differences observed between the strains may be the result of a higher capacity for absorbing the nutrient by P. tricornutum Ub7, specified by the excessive consumption, or to different physiological conditions of the cells that interfere with their metabolic and chemical composition relations.
Perhaps the incorporation of organic phosphorus and the modification of cell volume and length are mechanisms used by Ub7 to increase the orthophosphate cell quota, even in P-enriched conditions.Batch cultures undergo continual changes in their chemical composition, especially with regard to nutrient concentration, which are reflected in metabolism and cellular biochemistry and, consequently, changes in surface/volume ratio and other physiological interactions that occur during different growth stages of a microalgae.