Levels of neutral detergent fiber in diets with forage palm for dairy goats

This study aimed to evaluate the amount of fiber in the diet that is able to promote better use of nutrients in the diet of dairy goats when forage palm is the main source of roughage. Fifteen Alpine dairy goats were distributed in a 5 × 5 triple Latin square containing five treatments and five periods, and each treatment contained. We tested five diets composed of different contents of neutral detergent fiber from Tifton hay (NDFf) in the dry matter of rations containing forage palm: D1: 11.98%; D2: 18.31%; D3: 23.68%; D4: 28.76% and D5: 32.12% NDFf. The intake of dry matter, crude protein, neutral detergent fiber, non-fiber carbohydrates and ether extract decreased linearly with the increase in NDFf content. There was a linear increase of milk production and milk protein with increasing dietary NDFf. The fat, dry extract, protein, pH and acidity of the evaluated milk were not affected by increasing the levels of NDFf in the diet. In situations where dairy goats are fed forage palm and other types of forage are limited, 11.98% NDF from forage other than palm can be used as an effective source of NDF.


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The supply of forage for ruminants in semiarid regions is based on the use of crops adapted to water stress, such as the forage palm, which guarantees the maintenance of animal productivity during the dry season.Forage palm Arq.Bras.Med. Vet. Zootec., v.70, n.5, p.1595-1604, 2018 The presence of oxalate in the palm has been considered as a diarrheal cause in ruminants.However, forage palm has around 0.16% of oxalate based in dry matter, very below the level that is necessary to cause toxicity, which is 2% of dry matter (Batista et al., 2009).Therefore, other reasons need to be need investigated.
Ruminants require a minimum amount of fiber in the diet to maintain adequate ruminal fermentation and, consequently, animal health.It is necessary that part of this fiber should come from forage, because neutral detergent fiber from forage (NDFf) stimulates the chewing activity and saliva production, which maintain the buffering capacity of rumen (Nutrient…, 2001).Various parameters have been used to monitor ruminal conditions including milk fat percentage (Nutrient…, 2001).Effective fiber is the amount of fiber in the diet that maintains the percentage of fat in the milk (Nutrient…, 2001).
Based on numerous studies with cows fed diets containing alfalfa and concentrate, it was found that diets with 25% NDF, 19% NDFf and 44% non-fiber carbohydrates (NFC) result in production and composition similar to the milk of cows fed high NDF concentrations (Mertens, 1997).In balancing rations for goats (mainly dairy goats), these studies are used to recommend the minimum amount of NDF in the ration.
In diets that contain forage palm as the main ingredient, it was found that palm does not contain sufficient NDF to be effective in maintaining animal performance and good rumen health (Ferreira et al., 2010).One way to overcome this problem is the inclusion of other sources of NDF in the diet (Ferreira et al., 2010).However, it is uncertain how much more NDF from other sources of forage is necessary to maintain animal performance (Ferreira et al., 2010 andWanderley et al., 2002).
Thus, the objective of this study was to evaluate the amount of fiber in the diet that is able to promote better use of nutrients in the diet of dairy goats when forage palm is the main source of roughage.

MATERIAL AND METHODS
The experiment was conducted from May to August 2012 at the Estação Experimental Pendência, which belongs to the EMEPA-PB, Empresa Estadual de Pesquisa Agropecuária da Paraíba, located in the rural area of Paraíba, micro-region of West Curimataú, municipality of Soledade -PB, Brazil (7º 8' 18" S and 36º 27' 2" W), with an altitude of 534m.The bromathological analyses were performed at the Laboratory of Animal Nutrition, Department of Animal Science, Federal University of Paraíba (UFPB), located in Areia-PB, Brazil.
Fifteen multiparous alpine dairy goats with a mean body weight (BW) of 43.09±1.94kg,at ±30 days in milk at the start of the study, were used.The experimental design used was a triple Latin square (5 x 5), with five treatments and five periods, balanced in accordance with the lactation period and milk production of the goats.Each animal was housed in an individual "tie" type stall and provided with a feeder and drinker, where they received their diet.The experiment was approved by the Committee of Ethics in the Animal Using -CEUA -CBiotec of the Federal University of Paraíba (UFPB), with protocol number: CEUA N o 2105/13.The diets consisted of forage palm, Tifton hay, and concentrate supplement, and the treatments represented diets with different levels of NDF from Tifton hay (NDFf) in the dry matter: D1: 11.98%; D2: 18.31%; D3: 23.68%; D4: 28.76% and D5: 32.12% NDFf.The Tifton hay used in the present study was obtained from a local commercial farm.Bales of hay were chopped in a stationary tub grinder to a geometric mean particle length of 20.0mm.
The diets were formulated to be isonitrogenous and to meet the requirements of the NRC (2007) for goats weighing on average 45kg and producing 2.5kg of milk per day with 4.0% fat (Table 1) and (Table 2).
The experiment consisted of five periods lasting 21 days each, with 14 initial days for diet adaptation and the rest for data collection.The animals were confined and fed twice per day (8:00 a.m. and 3:30 p.m.) in equal proportion, and the food was supplied individually as total mixed rations.The diets were provided ad libitum, so that there were at least 10% leftovers, which were weighed daily so that the food supply level was properly adjusted.Samples of the supplied food and the leftovers were collected during the seven days of collection and then weighed.Composite samples were made of the samples for each trial period to determine the digestible dry matter content, digestible organic matter, digestible crude protein, digestible ether extract, digestible nonfiber carbohydrates and digestible neutral detergent fiber.The analyses followed the conditions described in the AOAC (Official…, 1998), except for the neutral detergent fiber (aNDFom).The aNDFom contents were estimated according to Mertens' recommendations (2002) and with corrections regarding ash and protein contents in the aNDFom as recommended by Mertens (2002) and Licitra et al. (1996).
The energy value of the diets (TDN) was obtained through data from the apparent digestibility observed in the experiment and calculated according to Sniffen et al. (1992) Milk production was measured daily, and the average of the last seven days of each animal within each period was used for statistical analysis.Composite milk samples were collected in the morning milking (120mL) and afternoon milking (80mL) to determine the milk composition (pH, density, moisture, lactose content, dry extract, protein, and fat) of each animal within each period.The milking and handling procedures followed the recommendations of the Technical Regulation of Production, Identity and Quality of goat milk (Brasil, 2000).
Feed efficiency was determined by dividing the milk production by the dry matter intake.
The ingestion behavior of the animals was determined on the 14th day of each trial period through individual observation of the time spent in feeding, rumination and idleness time over 24 hours.For this purpose, we used the instantaneous scanning method proposed b Martin and Bateson (1986), at intervals of five minutes (Johnson and Combs, 1991).The following variables were observed and recorded: total feeding time (TFT): the period in which the animal grasped the food, chewed, salivated and swallowed the bolus; Total Rumination (TR): the period in which the animal regurgitated, rechewed and swallowed the regurgitated material; and Total Idleness (TI): the period in which the animal did not eat or ruminate.
The measurement of water intake was performed through weighing of water twice daily (5:30 a.m. and 3:30 p.m.) between the 15th and the 21st days of the experimental period.Water was provided ad libitum, and its consumption was estimated by difference of weight.The daily evaporation rate was estimated by distributing buckets at different parts of the shed, so that the amount of water lost by evaporation could be verified on the next day and thus these losses were discounted from the consumption of animals.
The effects of the NDFf levels were evaluated by regression analysis using the determination coefficients and significance of regression parameters as the model choice criteria.These parameters were tested using a t-test at a probability level of 5%, using the GLM procedure of SAS (Statistical…, 2017).The statistic model was defined as follows: Yijkl = μ + A(k)i + Pj + Qk + Tl + QTkl + ξijkl where: Yijkl = Observation of animal i (random effect), in period j (random effect), on square k (random effect), subjected to treatment l (fixed effect); μ = overall effect of the mean; A(k)i = effect of animal i on square k, with i = 1, 2, 3, 4, 5; Pj = effect of period j; Qk = effect of the Latin square, with k = 1, 2, 3; Tl = effect of treatment l, with l = 1, 2, 3, 4, 5; QTkl = interaction of the effect with the Latin square × treatment l; and ξijkl = random error associated with each observation Yijkl.
The intake of supplied and total water increased linearly with the increase in the concentration of NDFf in the diet (Table 4), while water intake via the diet showed a decreasing linear effect.
The digestibility of DM and other nutrients showed increasing linear effects with the varying levels of NDFf in the diet (Table 5).465+0.415x 1 11,98= 11,98% of neutral detergent fiber from Tifton hay; 18,31= 18,31% of neutral detergent fiber from Tifton hay; 23,68= 23,68% of neutral detergent fiber from Tifton hay; 28,76= 28,76% of neutral detergent fiber from Tifton hay; 32,12= 32,12% of neutral detergent fiber from Tifton hay; 2 OWI= Offered water intake, DWI= diet water Intake, TWI= total water intake. 3Quadratic.The time spent on ingestion was not influenced (P> 0.01) by the diet (Table 6).However, the time spent for rumination increased linearly with increasing concentrations of NDFf.There was a decreasing linear effect of the levels of NDFf on time spent on idleness, on the feeding efficiency values of DM and NDF and on the rumination efficiency of DM and NDF.

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There was a linear effect of the NDFf in the diet on the production of goat milk (Table 7).The milk production of the animals consuming 11.98% NDFf in the diet was 0.15kg/day less than the animals consuming 32.12% NDFf, leading to a decrease of 9% in daily milk production.The linear increase of NDFf in the diet was also observed when the milk was corrected to 4% fat.However, the decrease in milk production in the range from 32.12% to 11.98% NDFf was lower (7%).-4.820x 1 11,98= 11,98% of neutral detergent fiber from Tifton hay; 18,31= 18,31% of neutral detergent fiber from Tifton hay; 23,68= 23,68% of neutral detergent fiber from Tifton hay; 28,76= 28,76% of neutral detergent fiber from Tifton hay; 32,12= 32,12% of neutral detergent fiber from Tifton hay; 2 TFT= total feeding time; TR= total rumination; TI= total idleness; FE= feeding efficiency; RE= rumination efficiency. 3Quadratic.
The feeding efficiency improved linearly with the increase in NDFf content in the diet (Table 7).

DISCUSSION
According to Dado e Allen (1994), the consumption of rations with at least 28% NDF may be limited by rumen capacity.Whereas all the rations of this experiment show NDF content higher than the value mentioned above (Table 2), the filling effect may have influenced the consumption of the rations.
The decrease in DM intake (Table 3) influenced the linear reduction in the intake of all nutrients (CP, EE, NFC, NDF) and ME.Thus, the decrease in the DM intake decreased the CP and EE intakes once the diets were formulated to be isonitrogenous and had similar levels of EE.Increasing concentrations of NDFf produced a reduction of NFC content in the ration, and with the decrease in the DM intake, there was a greater reduction of the intake of this nutrient.Despite the increase of aNDFom content with higher concentrations of NDFf, the reduction in DM intake produced a greater effect on the NDF intake.The ME comes from the sum of available energy in those nutrients; thus, as there was a decrease in the intake of them all, there was also a reduction in ME.
For dairy goats producing 2.0kg/day of milk, the requirements for CP and TDN according to the NRC (Nutrient…, 2007) are 229.86g/dayand 1219.42g/day,respectively.Only the animals subjected to diets with 11.98 and 18.31% NDFf met these requirements (Table 2).This demonstrates that the filling effect had more influence on the reduction of intake than the energy concentration of the ration.
With regard to water consumption, increasing the NDFf content increased the DM content (Table 2).The DM content of the diet is one of the main factors affecting water intake (Nutrient…, 2001(Nutrient…, , 2007)).This influenced the increase in water consumption in the animals that consumed higher concentrations of NDFf in the diet (Table 4).
However, in semi-arid regions, a limited water supply can be an obstacle to livestock production.Thus, the lower intake of supplied water by the animals that receive lower NDFf concentrations in the diet can be beneficial (Oliveira et al., 2007).
The increased digestibility of NDF of rations with higher fiber content was also observed by Kendall et al. (2009) in dairy cows.Branco et al. (2011), working with dairy goats, found a decrease in the digestibility of DM, OM, and NFC when the NDFf content was increased in the diet.However, Branco et al. (2011) worked with higher levels of NDFf in the diet compared to this study.
The excess of NFC in diets for ruminants can also cause metabolic diseases (Nutrient…, 2001).Diets with high concentration of forage palm, as observed in this work, has high concentrations of NFC (Table 2).Thus, the presence of NDF improves digestion and animal health.
The increasing linear effect of the digestibility of DM, CP, NDF, EE, NFC and ME (Table 5) when increasing the concentration of NDFf in the diet may be due to lower DM intake and to the longer retention time of fiber in the rumen.This allowed the nutrients to stay in the rumen for a longer period to be fermented by ruminal microorganisms, which consequently allowed additional nutrients to be absorbed in the gastrointestinal tract.Kendall et al. (2009) observed through markers that the outflow rate of cows decreased when NDF content was increased from 28% to 32% in the diet.
The retention time of fiber in the rumen is related to the time required to reduce the size of the food particles by rumination, so that they can pass through the reticulo-omasal orifice.Forage fiber is one of the components that stimulate rumination (Krause et al., 2002).Table 6 demonstrates that increasing the NDFf content in the diet increased the rumination time.Tavares et al. (2005) also observed this behavior in goats.
The sum of the lower DM and NDF intakes and longer rumination time when increasing the NDFf content in the rations probably caused the feeding and rumination efficiency of both DM and NDF to decrease (Table 6).The values observed in this experiment behaved similarly to Branco et al. (2011) andCarvalho et al. (2006).
The MY, FCM4% and FE of the goats increased linearly with the increasing concentration of NDFf in the diets with forage palm (Table 7), even with the decrease in nutrient intake.There was also an increase in rumination activity with the increase in NDFf content and, consequently, in chewing.NDF increases chewing activity, which increases salivary secretion, preventing the decline of ruminal pH (Yang e Beauchemin, 2005).Bispo et al. (2007) observed that the use of forage palm replacing elephant grass hay in the sheep diet changed ruminal pH.Animals fed a non-palm diet had an average pH of 6.46 while in diet with 56% of forage palm the pH decreased to 6.24.A drop in ruminal pH changes the microbial population, reducing the digestibility of nutrients (Véras et al., 2000).The behavior of the digestibility of nutrients observed in this study (Table 5) demonstrates this.This lower digestibility when there are lower concentrations of NDFf in the diet is probably due to a disadvantage of rumen function and digestion of fiber, worsening the energy and protein ratio and decreasing microbial protein synthesis in the rumen (Yang e Beauchemin, 2005).On the other hand, the high ruminal degradability maximizes the fermentation capacity of the rumen, thus increasing microbial protein synthesis, the production of volatile fatty acids and, as a consequence, the conduction of nutrients to the animal (Ferreira et al., 2010).
The higher microbial protein synthesis in the rumen may have also increased the amount of digestible intestinal protein which was used by the animal for milk protein synthesis.This may have been reflected in the higher production and protein content in the milk when the concentration of NDFf was increased in the diet (Table 7).
Besides, the current study the NFC of the diets with 11.98% and 18.31% of NDFf content exceeded maximum recommendation (44% of NFC).Diets with excess of NFC cause depression milk production as a result ruminal upsets and health problems (Nutrient…, 2001).However, even when the NDFf concentration was lower in the diet, all tested levels provided protein values recommended by the current legislation in Brazil (IN 37), which is at least 2.8% in the milk (Brasil, 2000).
Likewise, the concentrations of total solids, lactose, fat, acidity and pH of the milk maintained values that met current regulations (Brasil, 2000), regardless of the concentration of NDFf in the diet of the animals (Table 7).Branco, et al. (2011) suggested that 35% NDF from forage is the concentration at which the best efficiency in the use of forage in rations for dairy goats is obtained; beyond this point, milk production reaches a plateau.In this study, all the rations presented NDFf content lower than 35%.This range explains the increasing linear effect on milk production when the concentration of NDFf increased in the diet.
According to the NRC (Nutrient…, 2001), diets with less than 250g NDF.Kg -1 DM and 190g NDFf.Kg -1 DM depress the percentage of fat in cow's milk.In balancing goat rations, the NRC (Nutrient…, 2007) also uses these studies to recommend the minimum amount of NDF in the ration.
In the studied range from 11.98% to 32.12% NDFf, all the diets were able to maintain the percentage of fat in milk (Table 7), indicating that the recommendations of NRC (Nutrient…, 2007) and NRC (Nutrient…, 2001) do not apply to dairy goats fed forage palm.
Goats have anatomical and physiological variations that differentiate them from bovines.Goats have bigger salivary glands, a smaller rumen in relation to body weight, and anatomy for grasping food that favors the selection of more digestible food (Van Soest, 1994).Furthermore, goats have a faster outflow rate in the gastrointestinal tract than bovines (Van Soest, 1994).These differences can make goats more tolerant to use diets with low content of effective fiber and high concentrations of NFC.
In arid and semi-arid regions, during drought periods, one of the major restraints to livestock production is the amount of forage available other than palm.Using fodder in concentrations lower than those recommended by the NRC (Nutrient…, 2007) can favor milk production at this time of year, as it would allow more inclusion of palm in the formulation of the ration.The use of minimal amounts of NDFf could help the farmer to keep the flock with lower production, while maintaining the quality of the goat milk, when adequate quantities of forage are not available on the property.

Table 1 .
Chemical composition of the ingredients contained in experimental feeds based on dry matter

Table 3 .
Intakes of chemical components by dairy goats fed neutral detergent fiber from tifton hay levels (FDNf)

Table 4 .
Intakes of water by dairy goats fed neutral detergent fiber from tifton hay levels(FDNf)

Table 5 .
Apparent digestibility coefficients of the nutrients and metabolizable energy of the diets in dairy goats fed neutral detergent fiber from Tifton hay levels(FDNf)

Table 6 .
Behavioral variables from dairy goats fed neutral detergent fiber from Tifton hay levels (FDNf)