Anti-inflammatory effect of lycopene on endotoxin-induced uveitis in rats

1 Department of Ophthalmology, Harran University, School of Medicine, Sanliurfa, Turkey. 2 Department of Medical Pharmacology, School of Medicine, Harran University, Sanliurfa, Turkey. 3 Department of Medical Biochemistry, School of Medicine, Harran University, Sanliurfa, Turkey. 4 Department of Medical Pathology, School of Medicine, Harran University, Sanliurfa, Turkey. Funding: This study was supported by grants from the Harran University Scientific Research Council (HUBAK-14112).


Conclusões: Estes resultados sugerem que o licopeno pode ter efeitos benéficos no tratamento da inflamação ocular, através dos seus efeitos anti-inflamatórios e an tioxidantes.
Descritores: Uveíte/induzido quimicamente; Endotoxinas; Estresse oxidativo; An tioxidantes; Inflamação; Citocinas; Animais; Modelos animais de doenças; Ratos those that may prevent or stop ocular inflammation (4) .Lipopolysaccharide (LPS), the main component of the cell walls of gram-negative bacteria, generates an acute inflammatory response in EIU.LPS is recognized by membrane-bound receptors, principally on the surface of macrophages, and triggers the release of proinflammatory factors, such as tumor necrosis factor-α (TNF-α), interlukin-6 (IL-6), nitric oxide (NO), and monocyte chemoattractant protein-1 (5) .The inflamma tory response to LPS also induces oxidative stress (6) .Eventually the initiated proinflammatory cascades results in the breakdown of the blood-ocular barrier and the infiltration of leukocytes into both anterior and posterior segments of the eye, which contributes to the further progression of EIU (4) .
Lycopene is a dietary carotenoid present in tomatoes and other red fruits and vegetables such as watermelon and pink grapefruit (7) .It has been reported that dietary intake of tomato and tomato-derived products can reduce the risks of chronic diseases and various cancers, especially prostate cancer (8,9) .Lycopene is a potent antioxidant as well as an inhibitor of proinflammatory and prothrombotic factors (10) , although the mechanisms for these additional activities have not been clarified.Lycopene was shown to inhibit formation of proinflammatory cytokines and chemokines in macrophages (11,12) .The inherent antioxidant activity of lycopene may contribute to immune system modulation (13,14) .
In the present study, we evaluated the efficacy of lycopene against EIU-associated inflammation and oxidative stress in rats by histopathologic and biochemical analyses.Another aim was to compare the anti-inflammatory effects of lycopene to those of dexamethasone, which is currently the standard treatment for uveitis.

animals and experimental protoCol
This study was conducted on 28 healthy, 6-8-week-old adult Sprague-Dawley rats weighing 240-275 g obtained from Dollvet Animal Laboratory (Sanliurfa, Turkey), where the experiments were also performed.The study was performed in accordance with National Institutes of Health guidelines for the Care and Use of Laboratory Animals and the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research.The study was also approved by the Dollvet Animal Care and Use Committee (Approval number: 2014/51).The animals were allowed to acclimate under standard laboratory conditions (12-h light; 12-h dark) in a room with controlled temperature (24 ± 3°C) for one week prior to experiments.The animals had free access to water and were fed a standard commercial pellet diet ad libitum.
Endotoxin-induced uveitis (EIU) was induced by a single subcutaneous injection of LPS (200 μg) dissolved in 0.1 ml sterile saline as described previously (4) .The dosing protocols were based on previous studies (15)(16)(17) .After seven days of acclimatization, the animals were randomly divided into four groups: Vehicle Control (vehicle only, n=6), vehicle pretreatment then LPS (Vehicle + LPS, n=6), lycopene pretreatment then LPS (Lycopene + LPS, n=7), and a positive control receiving de xamethasone pretreatment (DEX + LPS, n=7).In the Vehicle Control group, vehicle (0.9% NaCl) was administered by intraperitoneal injection for three consecutive days at 1 ml/injection, followed by 0.1 ml vehicle instead of LPS.The Vehicle + LPS group received vehicle as pretreatment then subcutaneous LPS injection on day three at 200 μg/0.1 ml.The Lycopene + LPS group received daily intraperitoneal injection of 10 mg/kg lycopene for three consecutive days, then subcutaneous injection of LPS at 200 μg/0.1 ml.The positive control DEX + LPS group received LPS at 200 μg/0.1 ml immediately after intraperitoneal injection of 1 mg/kg dexamethasone.
Twenty-four hours after LPS or final vehicle administration, eyes were examined under microscopy and enucleated.The aqueous humor was collected from both eyes (15-25 μl) by anterior chamber puncture with a 30-gauge needle under a surgical microscope.One of the eyes was fixed with 10% buffered formalin solution at room tempe-rature for histopathological evaluation, and the other was stored at -80°C for biochemical analyses.

BioChemiCal measurements
To count infiltrating cells, the aqueous humor samples were suspended in an equal volume of Turk stain solution (Merck, Darmstadt, Germany) and examined by a hemocytometer under light microscopy.The number of cells was obtained by averaging the results of four randomly chosen fields from each sample.The aqueous humor samples were stored in ice water until testing, and cell counts were measured on the day of sample collection.The microprotein level was measured by the Lowry method (18) .The concentrations of TNF-α, NO, and IL-6 were measured using commercially available rat ELISA kits.

analysis of oxidative stress markers in the aqueous humor and oCular tissue
The crystalline lens was removed from enucleated eyes due to the high glutathione concentration (19) .Ocular tissues were weighed, broken down into very small pieces and placed in empty glass tubes.Then, 1 ml of 140 mM KCl solution per gram of tissue was added to each tube, and all tissues were homogenized in a motor-driven ho mogenizer.The homogenate was centrifuged at 2,800 × g for 10 min at 4°C (20) .Total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured in supernatant and ocular tissue samples as described previously (21) .Briefly, TAS was measured spectrophotometrically using a commercial kit (Rel® Assay Diagnostics Mega Tıp San.ve Tic. Ltd.Sti., Gaziantep, Turkey).TAS measurements of aqueous humor are expressed as mmol Trolox equivalent/L and TAS measurements of ocular tissue as mmol Trolox equivalent/gram protein.TOS was also measured in samples spectrophotometrically using commercial kits (Rel® Assay Diagnostics, Mega Tıp San.ve Tic. Ltd.Sti., Gaziantep, Turkey) and expressed as micromolar hydrogen peroxide per liter (μmol H 2 O 2 equivalent/L) or μmol H 2 O 2 equivalent/gram protein as appropriate.OSI was defined as the percentage ratio of TOS to TAS.

CliniCal manifestations sCoring
Clinical features of ocular inflammation were evaluated in both eyes 24 h after LPS or final vehicle injection using a slit lamp.Severity was graded from 0 to 4 by an observer blind to treatment history as described previously (22) .The clinical grading was defined as follows: Grade 0 was defined as no obvious inflammatory response, Grade 1 as discrete dilation of the iris and conjunctival vessels, Grade 2 as moderate dilation of the iris and conjunctival vessels with moderate flare in the anterior chamber, Grade 3 as intense iridal hyperemia with intense flare in the anterior chamber, and Grade 4 as the clinical signs of Grade 3 plus fibrinous exudation and miosis.

histopathologiCal examination
For histopathologic analysis, enucleated eye specimens were dissected from anterior to posterior and fixed in 10% neutral formalin for 48 h.Routine tissue processing protocols were performed and tissues were embedded in paraffin.Three or five sections of 4-to 5-μm thickness were cut from each specimen and stained with hematoxylin and eosin.Histopathologic findings in the uveal structures and cornea were scored as described previously (23) .Grade 0 was defined as normal tissue.Grade 1 was defined as dilated iris vessels and thickened iris stroma with exudate, protein, and/or a few scattered inflammatory cells in the anterior chamber.Grade 2 was defined as infiltration of inflammatory cells into the stroma of the iris and/or ciliary body with a moderate number of inflammatory cells within the anterior chamber.Grade 3 was defined as heavy infiltration of inflammatory cells within the iris stroma, ciliary body, and the anterior chamber.Grade 4 indicated heavy exudation of cells, dense protein aggregation in the anterior chamber, and inflammatory cell deposits on the corneal en dothelium.Histopathologic analyses were performed by a single pathologist blinded to treatment history.

statistiCal analyses
Biochemical and histopathological measurements are reported as mean ± standard deviation.All group data were compared by non-parametric Kruskal-Wallis tests.The Mann-Whitney U test was used to compare paired groups.A p-value less than 0.05 was regarded as statistically significant.Data analyses were performed using the statistical software SPSS for Windows (version 18.0).

Cellular infiltration in the aqueous humor
The number of infiltrating cells in the aqueous humor 24 h after LPS injection was significantly elevated compared to the Vehicle Control group (946 ± 1125 cells/μl vs. 6 ± 16 cells/μl).Pretreatment with lycopene before LPS resulted in a substantial reduction of cell number to 342 ± 221 cells/μl, although the difference was not statistically significant (Vehicle + LPS vs. Lycopene + LPS, p=0.472).Pretreatment with dexamethasone (DEX + LPS Group) also reduced cell number, which was statistically significant (120 ± 71 cells/μl, p=0.004).The mag nitude of the mean lycopene effect was comparable to that of dexamethasone (p=0.111)(Figure 1 A).

protein ConCentration in the aqueous humor
The protein concentration in the aqueous humor of the Vehicle + LPS group was significantly elevated compared to Vehicle Control (11.0 ± 3.1 mg/dl vs. 1.5 ± 1.5 mg/dl) and was not significantly reduced by lycopene pretreatment (Lycopene + LPS: 8.4 ± 6.0 mg/dl, p=0.534), while dexamethasone pretreatment (DEX + LPS) did significantly reduce protein concentration in the aqueous humor compared to the Vehicle + LPS group (1.9 ± 0.1 mg/dl, p=0.002).Thus, the efficacy of lycopene was less than that of dexamethasone (p=0.022)(Figure 1 B).

no ConCentration in the aqueous humor
In the Vehicle + LPS control, NO concentration in the aqueous humor was also higher than in the Vehicle control group (0.29 ± 0.1 μM vs. 0.14 ± 0.0 μM) and again was significantly reduced by lycopene pretreatment (0.19 ± 0.1 μM, p=0.003) and dexamethasone pretreat ment (0.21 ± 0.06 μM, p=0.043).The inhibitory effect of lycopene on NO accumulation was comparable to that of dexamethasone (p=0.352)(Figure 2 B).

il-6 ConCentration in the aqueous humor
The IL-6 concentration in the aqueous humor was higher in the Vehicle + LPS group than in the Vehicle Control group (121.6 ± 3.0 pg/ml vs. 108.9± 7.9 pg/ml) and, consistent with effects on NO and TNF-α, was significantly reduced by lycopene (111.1 ± 5.6 pg/ml, p=0.008) and by dexamethasone pretreatment (111.9 ± 4.3 pg/ml, p=0.004).The effect of lycopene was comparable to that of dexamethasone (p=0.666)(Figure 2 C).

oxidative stress markers in the aqueous humor
The mean OSI in the aqueous humor was higher in the Vehicle + LPS group than in the Vehicle Control group (14.5 ± 0.3 AU vs. 8.9 ± 2.2 AU) and was reduced by both lycopene (12.2 ± 3.6 AU, p=0.025) and dexamethasone pretreatment (11.9 ± 1.2 AU, p=0.008).The effect of lycopene was comparable to dexamethasone (p=0.657)(Figure 3 A).

oxidative stress indiCators in oCular tissue
Mean OSI in the ocular tissue was higher in the LPS-treated group than the Vehicle Control group (1.5 ± 0.1 AU vs. 0.9 ± 0.2 AU) and was reduced moderately but not significantly by lycopene pretreatment (1.2 ± 3.6 AU, p=0.775).In contrast, dexamethasone pretreatment significantly reduced OSI compared to the Vehicle + LPS group (1.0 ± 0.2 AU, p=0.004).The magnitude of the average lycopene effect was comparable with that of dexamethasone (p=0.101)(Figure 3 B).

histopathology sCores
No infiltrating cells were detected in the ocular sections of the Vehicle Control group.The histopathologic score 24 h after LPS injection (Vehicle + LPS group) was reduced significantly by lycopene pretreat ment (2.0 ± 0.0 vs. 0.4 ± 0.5, p=0.001).Similarly, dexamethasone pretreatment significantly reduced histopathologic grade (0.7 ± 0.5, p=0.002).The efficacies of lycopene and dexamethasone were comparable (p=0.409)(Figure 5).Lycopene, a natural dietary carotenoid, may reduce the risks of chronic diseases and various cancers (8,9) .It has been reported that lycopene reduces proinflammatory cytokine and chemokine expression in macrophages (11,12) , which may account, at least in part, for these health benefits.Accumulating evidence also suggests that modulation of intracellular gap junction communication, metabolic pathways, and other immune system functions contribute to lycopene efficacy (13,14) .Furthermore, lycopene suppresses inflammation in several tissues by inhibiting the formation of proinflammatory molecules such as NO, interleukins, and cyclooxygenase (9,11,12) .
Uveitis is an inflammatory intraocular disorder afflicting the uvea, a highly vascular tissue.Due to as yet unclear etiopathological factors for formation and progression, there is no definitive treatment for autoimmune uveitis.Corticosteroids are currently the primary treatment choice, but steroid use is associated with serious complications, including glaucoma and cataract (24,25) .The identification of new anti-inflammatory compounds is thus required to reduce the complications induced by corticosteroids.Accordingly, numerous clinical and experimental studies have been conducted evaluating nonsteroidal treatment strategies for uveitis (15,(26)(27)(28) .Endotoxin-induced uveitis is a useful model for this purpose.LPS stimulates the inflammatory response by activating expression and release of inflammatory cytokines such as TNF-α, IL-6, and monocyte chemoattractant protein-1, as well as NO (5) .In addition to direct activation of inflammatory cytokines by LPS, the resulting oxidative stress is known to modulate inflammatory signaling (6) .Furthermore, oxidative damage is exacerbated by NO ge ne ration.Thus, we speculated that lycopene, a known immunosuppressant and antioxidant, would suppress uveitis.
In the present study, we demonstrate for the first time that lycopene has significant anti-inflammatory efficacy in the rat EIU model.Cell count and protein concentration in the aqueous humor are useful indicators of blood-aqueous barrier integrity in EIU, and both were elevated by LPS.Also consistent with previous uveitis studies (5) , TNF-α, NO, and IL-6 concentrations were elevated in the aqueous humor of LPS-induced EIU rats, and the levels of all three factors were reduced by lycopene pretreatment.In fact, the magnitudes of these reductions were comparable to dexamethasone, the primary clinical treatment against uveitis.Consistent with suppression of inflammatory factors, the clinical severity and histological signs of inflammation were improved substantially by lycopene.While lycopene was not as effective as dexamethasone in reducing cell count and protein con centration in the aqueous humor, 10 mg/kg lycopene was as effective or better than 1 mg/kg dexamethasone for reducing histopathological signs (as evidenced by the slightly greater reduction in histopathological grade).Lycopene pretreatment lowered OSI in both the aqueous humor and ocular tissue, although the effect was significant only in the aqueous humor, possibly due to limited tissue access.Given that oxidative stress contributes to the pathogenesis of LPS-induced inflammation, these reductions may explain the improved clinical and histopathological signs of inflammation.
In mice, the anti-inflammatory effects of lycopene against LPS sti mulation depend on inhibition of NO and IL-6 production as well as suppression of NF-kB transcriptional activity and associated downstream pathways (29,30) .Our findings are consistent with these results, confirming that the anti-inflammatory activities of lycopene are mediated by inhibition of TNF-α, NO, and IL-6 production, resulting in reduced uveal inflammation.
Limitations of this study include the lack of pharmacokinetic, mechanistic, and functional analyses.We only assessed a brief three-day pretreatment protocol and did not measure intraocular lycopene levels.Such measurements could explain the differences in antioxidant efficacy between vitreous humor and tissue.We also did not evaluate the dose-response relationship, studies needed to define the optimal dosing protocol.While relief of oxidative stress is expected as carotenoids are free-radical scavengers, we did not assess specific mechanisms underlying the anti-inflammatory effects.In addition, we did not exa mine the reasons for greater dexamethasone efficacy against spe cific pathological indices, particularly blood-ocular barrier breach.Finally, functional recovery of vision was not assessed.
In conclusion, our study demonstrates for the first time that systemic administration of lycopene can attenuate inflammation and oxidative stress induced by LPS in a rat model of EIU.In addition, we show that most benefits of lycopene treatment are comparable to those of *p<0.05 and **p<0.01significantly different from the Vehicle + LPS group.
.01 significantly different from the Vehicle + LPS group.

Figure 1 .
Effects of lycopene on cell infiltration and protein accumulation in the aqueous humor following lipopolysaccharide (LPS)-in duced uveitis.Lycopene pretreatment (Lycopene + LPS group) reduced cell infiltration A) and mildly suppressed protein exudation B) in aqueous humor compared to vehicle (Vehicle + LPS group).Dexamethasone pretreatment was used as a positive control (DEX + LPS).All data are shown as mean ± standard deviation.

Figure 3 .
Figure 3.Effect of lycopene pretreatment on oxidative stress index (OSI) in the aqueous humor following LPS injection.Lycopene pretreatment caused a significant reduction in OSI in the aqueous humor (A) compared to vehicle (Vehicle + LPS).Lycopene also reduced OSI in ocular tissue (B) compared to vehicle but the effect did not reach significance (p>0.05).All data are shown as mean ± standard deviation.A B

Figure 4 .
Figure 4. Effect of lycopene on clinical manifestations of intraocular inflammation.Ocular inflammation was evaluated by mi croscopic examination 24 h after lipopolysaccharide (LPS) injection.No inflammatory reaction was observed in vehicle-treated controls (A).Anterior segment examination revealed an inflammatory reaction consisting of hyperemia and edema associated with miosis and fibril formation in the anterior chamber following LPS (B).The inflammatory reaction was suppressed by pretreatment with lycopene (C) and by pretreatment with dexamethasone (D).Lycopene pretreatment caused a significant improvement in clinical score compared to vehicle following LPS (E).All data shown as mean ± standard deviation.C 05 significantly different from the Vehicle + LPS group.

Figure 5 .
Figure 5.The effect of lycopene pretreatment on histopathological signs of intraocular inflammation following LPS.No inflammatory cells were observed in the Vehicle Control group (A).Numerous inflammatory cells (black arrows) infiltrated the extravascular uveal tissue in the Vehicle + LPS group (B).Inflamma tory cellular infiltration was reduced by pretreatment with lycopene (Lycopene + LPS) (C) or dexamethasone (DEX + LPS) (D).Hematoxylin and eosin staining, magnification ×200.Lycopene pretreatment significantly improved clinical scores compared to vehicle pretreatment (E).All data shown as mean ± standard deviation.