Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase

Purpose: To investigate the effect of melatonin on uterine tissue in the ovariectomized rat model. Methods: Fourty Wistar albino rats were divided into four groups for histologic and immunohistochemical examination. The rats were first numbered randomly and then randomly divided into 4 equal groups: control (group 1), torsion (group 2), torsion+detorsion (group 3) and torsion+detorsion+melatonin (group 4) groups. In addition, four Wistar albino rats were used for western blot analysis in each group. And also, malondialdehyde (MDA) levels were measured biochemically in all rats. Results: The histopathological examination of the uterine tissue in rats ovarectomized showed a degeneration in uterine glands, dilation of blood vessels in the internal layer with a thrombosis and bleeding, abnormal nucleuses and vacuolated cytoplasm above and below the nucleus. In torsion group, the apoptotic cells increased in luminal epithelium and gland cells. In the melatonin group showed that the Bcl2 negative effect on the uterine epithelium and did not lead to apoptotic cells. Conclusion: The increase in vascular endothelial growth factor expression resulted in the rearrangement of endothelial cell growth and the induction of angiogenesis.

it was reported that the effects of melatonin on uterine structure and function [20][21][22] .Dair et al. 23 have reported that melatonin affects some morphological properties of the rat endometrium, particularly on the success rate of embryo implantation.It is explained that the underlying mechanisms of this effect may develop in two different ways.It has been reported that either directly acting on the melatonin receptors or indirectly due to a disorder of ovarian steroids.Similarly, melatonin has been reported to be effective on adrenal cortex in pinealectomized rats, especially in fascicular and reticular regions.In the pinealectomized group, VEGF-A expression was higher than the other groups.These results have revealed the increase in percentage of apoptosis 24 .Soares et al. 25 have reached the conclusion that the increase in the number and activity of interstitial cells may be a consequence of hormonal disturbances after pinealectomy and that it is responsible for increasing estrogen blood levels and delaying the transition to metaphores.Romeu et al. 26 have founded that pinealectomized samples presented signals of proliferation on ovarian surface epithelium and interstitial cells as well as high expressions of proliferating cell nuclear antigen and VEGF in rats.And, they concluded that melatonin may an important role in proliferation in ovarian structures and increase the number of luteal bodies as well as the levels of progesterone receptor.
Vascular endothelial growth factor is a multifunctional cytokine that stimulates angiogenesis and increases microvascular permeability through binding to specific receptors expressed on vascular endothelial cells 27,28 .The Bcl-2 gene encodes a membrane protein localized to the nuclear membrane, the inner surface of mitochondria, and the endoplasmic reticulum 29 .It is the important gene of the Bcl-2 family and has been shown to be an inhibitor of apoptosis 30,31 .
The purpose of this study was to

■ Introduction
Melatonin hormone secretion is produced by norepinephrine (NE)-driven pulses released by the intraparenchymal nerve fibers.This NE-releasing activity and therefore the working principle of the pineal gland is activated in the dark and inhibited by light.The dark-light conditions are transmitted to the suprachiasmatic nucleus, which is blocked by light by the eyes and actuated in the dark as a result of the absence of such an obstacle [1][2][3][4] .Melatonin and its metabolites are powerful free radical scavengers and broad spectrum antioxidants.Up to now, It has been investigated for the effects of melatonin on endometriosis in rats or mice in terms of expression of Bcl-2 and VEGF in recent years [5][6][7][8][9][10][11] .Studies in animals and human have shown a significant effect of melatonin on cell death in different tissues and systems, including the urogenital system.It appears that melatonin plays a modulatory role in apoptosis and may offer both pro-apoptotic and anti-apoptotic actions [12][13][14][15] .Several studies have indicated that antioxidant defences may be altered in endometriosis, as suggested by the aberrant expression of endometrial antioxidant enzymes and lower levels of the antioxidant vitamin E in peritoneal fluid 16,17 .Melatonin plays also a role in neuroendocrine regulation, the increase of immunity, the neutralization of free radicals, the reduction of angiogenesis, the increase of apoptosis, studies on animals and humans demonstrating that melatonin has important oncostatic properties.Blood melatonin levels are reversely correlated with the tumor proliferation index in patients with endometrial cancer 18 .The emphasis of immunological elements has also shown that melatonin can inhibit apoptosis by inducing the release of cytokines such as interleukin-4 19 .It is known that melatonin interacts with nuclear receptors.This interaction has been shown to alter the gene expression of the inducers or inhibitors of apoptosis.In several studies, evaluate immunoexpression of vascular endothelial growth factor and Bcl-2 in the endometrium of rats treated with melatonin in the estrus phase.

■ Methods
All animal experiments and the consequent care and healing of the animals utilized as a part of this investigation were in strict understanding with the National Institutes of Health (NIH Publications No. 8023, revised 1978) rules for animal care.All procedures performed in this experiment were approved by the Ethics Committee for the Treatment of Experimental Animals (University of Dicle, Faculty of Medicine, Turkey, Protocol number: 2017-12).
Totally fifty-six Wistar albino rats (180-210 g, 12 weeks old) were kept up under 22±1°C and 12 hrs light/gray cycles with ad libitum access to standard pelleted nourishment and water during 7 days.All rats at the end of experiment were healthy and no difference in food/water consumption and body weight gain between experimental and control rats were observed.Totally fifty rats were numbered and then divided into 4 equal groups randomly (n=10): Control (Group 1), Torsion (Group 2), Torsion+Detorsion (Group 3) and Torsion+Detorsion+Melatonin (Group 4) groups for histologic and immunohistochemical examination.In addition, four rats in each group were used for Western blot analysis.Following acclimation, the stage of the estrus phase of the rats was evaluated by performing daily vaginal smears.The rats determined to be in estrous were weighed and prepared for the surgical procedure.Each rat was administered intramuscular ketamine hydrochloride (50 mg/kg Ketamin hydroxide) and xylazine hydrochloride (10 mg/kg Rompun; Bayer, Istanbul, Turkey) for anesthesia 32 .1ml preoperative blood sample was drawn from the tail of each rat for the measurement of hormone levels.In all of the groups, a midline abdominal incision of 2,5 cm (laparotomy) was performed under sterile conditions.In the control group, the uterine horns and adnexa were observed for 1 minute.In the torsion+detorsion group, bilateral adnexal torsion (3-hour ischemia) was induced.The adnexal torsion procedure was carried out as follows: the adnexal was rotated 360°in a clockwise direction, including tubal and ovarian vessels and then fixed to the abdominal wall.In the detorsion group, detorsion (3-hour reperfusion) was performed following the 3-hour torsion 32 .The uterus tissues were fixed in 10% neutral buffered formalin solution for 24 h, dehydrated, cleared and embedded in paraffin as usual.Serial tissue sections at a thickness of 4-5 μm were cut using the microtome for the histopathological examinations.

Western blot analysis
The protein expression levels of VEGF and Bcl-2 were examined by Western blotting.Briefly, proteins were extracted from uterine tissue, separated on 10-12% SDS-PAGE gels (40 mg/lane) and then transferred to nitrocellulose membrane (Bio-Rad, Hercules, CA).Four uterine tissue were blocked with 5% non-fat milk in TBST buffer (10 mmol/L Tris-HCl, 0.15 mol/ L NaCl, and 0.05% Tween 20, pH 7.1) for 2 h and incubated with primary antibodies overnight at 4 C in all groups 33 .Primary antibodies used here were monoclonal mouse antibodies against VEGF (1:100, lot#MA5-12184, Thermo Fischer, Fremont, CA, USA), and Bcl-2 (1:1000 dilution; Cell Signaling Technology, Boston, MA).After extensive washing with TBST buffer, the blotted membranes were then incubated for 1 h at room temperature with an HRPconjugated secondary antibody directed against rabbit IgG (1:2,000; Cell Signaling).The proteins were detected using an enhanced chemiluminescence system (ECL kit, Pierce Biotechnology, Beijing, China) and captured on light sensitive X-ray film (AGFA, Belgium).
Optical densities were detected using ImageJ software.β-actin was used as the loading control (Figure 1).

Analysis of MDA in rat tissues
Malondialdehyde levels (MDA) determine levels of deterioration of lipid peroxidation mechanism.MDA is usually measured by derivation with thiobarbituric acid to give a red compound with an absorption maximum at 532 nm.The reaction was performed at pH 2-3 and 900°C for 15 min.The sample was mixed with two volumes of cold 10% (w/v) trichloroacetic acid to precipitate the protein.The precipitate was pelleted by centrifugation and a portion of the supernatant was reacted with an equal volume of 0.67% (w/v) TBA in a boiling water bath for 10 minutes.Ovarian tissue samples were homogenized with super cold 150 mMKCl for the assurance of MDA levels.After cooling, the absorbance was read at 532 nm.The MDA levels were tested and the outcomes are expressed as nmol MDA/g tissue (Table 1) 34 .

Statistical analysis
In this study, descriptive statistical average, the standard error values are given with standard deviations and averages.The normal distribution assumption Kolmogorov-Smirnow test fit of the data, while the homogeneity was examined by Levene test.The comparison of the average Intergroup One-way analysis of variance (ANOVA) test was used.Intergroup for multiple comparisons test, Dunnett and Tukey HSD tests were used.All statistical tests used is the 95% confidence interval; p value of <0.05 were considered statistically significant.

Biochemical findings
MDA levels were compared in control and experimental groups.The comparison of all groups with each other in terms of MDA (tissue and plasma) was statistically significant (Table 1).Groups were compared in terms of length of the uterine epithelium in all groups (Table 2).The average length in parameters showed statistically significant difference (F = 38.847,*P <0.001).

Histomorphometric analysis
In our study, we observed that the length of epithelium and the diameter of uterinal glands were decreased bilaterally in torsion group (Tables 2 and 3).The length of the uterine luminal epithelium and diameter of the uterine glands in melatonin administrated group were similiar as in control group.In terms of the average diameter of uterine gland parameter has changed in a statistically significant manner (F = 29.186,*P <0.001).Multiple comparison values between the groups is given in Table 3.

Histological findings
The morphologic characteristics of the uterus tissues were normal in the control group.
The uterus from sham operation group were normal in histopathological appearance.In the torsion group; Light microscopic examination of cervical degeneration mostly occupied endometrial uterine lumen and the lamina propria, revealed the significant proliferation.An increase in inflammatory cells in the lamina propria, vascular dilatation and hemorrhage, degeneration and apoptotic cells in the uterine glands was observed.Lamina propria and between the muscle layer showed a dilatation and hemorrhage increase in irregular veins.In the ovariectomized rats, reductions in the sizes of both the uterine epithelium and the endometrial glands were observed, as well as a loss of connective tissue.

Immunohistochemical findings
In the torsion group, increased VEGF expression in vascular endothelial and inflammatory cells.Torsion and detorsion group showed VEGF-positive reaction.In torsion group , the apoptotic cells increased in luminal epithelium and gland cells.Apoptotic Bcl2 cells were stained as positive.Bcl2 negative expression in endometrial epithelium, Bcl2 positive reaction showed in the cells around uterinal glands.In the uterine epithelium, it was observed in the protection effect of melatonin.

■ Discussion
The present experimental study aimed to investigated whether melatonin administration is useful or not in the prevention of endometrial damage subjected to ovarian torsion in rats followed by the histological, immunohistochemical and western blotting analysis.For the preservation of the ovarian function, it is important that the ovarian torsion and subsequent detorsion be done surgically quickly.Detoxification applied to protect the ovaries leads to biochemical and histopathological changes in the ovarian tissue.Because ovaries do not determine the degree of ischemia in the intraoperative procedure, detorsion is recommended as conservative treatment in all patients with ovarian torsion 35.Ovarian torsion causes congestion, arterial thrombosis and necrosis.Blood flow to the ovaries is supplied through the vascular structures on the supportive tissues and the torsion occludes these vascular structures, leading subsequently to ischemic damage on the ovarian cells 36 .Murray et al. 37 showed that OVX reduces the cell heights of the luminal and glandular epithelium in the sheep uterus.They also demonstrated that these changes are ameliorated by the coadministration of estrogen and progesterone after ovariectomy.Melatonin is a broad-spectrum antioxidant and therefore may interfere with the oxidative stress seen in endometriosis 38 .Melatonin is a powerful antioxidant with a influence on the endogenous antioxidant system.Concurrently, melatonin administration decreases lipid peroxidation and increases the levels of antioxidants 39 .MDA is a secondary product of oxidative stress formed during lipid peroxidation and is significantly increased by ıschemia-reperfusion injury.Melatonin also has an antiadhesive effect and decreases leukocyte-mediated endothelium injury 20 .The examination of the uterus for rat ovarectomized showed a degeneration in uterine glands; dilation of blood vessels in the internal layer with a thrombosis and bleeding; abnormal nucleuses and vacuolated cytoplasm above and below the nucleus VEGF has direct autocrine or paracrine effects on the luminal epithelial cells of the uterus during endometrial repair in the macaque monkey and mice 40,41 .Alterations in the expression of VEGF and its receptors are associated with disruption of ovarian and uterine functions 42 .In a study in mice, endometrial expression of VEGF mRNA changed throughout the estrous cycle, shifting from the luminal epithelium during the proliferative phase to the luminal stroma during the secretory phase, again indicating that ovarian steroids modulate endometrial VEGF expression 43 .Damous et al. 44 have investigated the safety and efficacy of treatment of adipose tissue stem cells (ASCs) in newly vaccinated ovaries 30 days after the injection.They found that the estrogen phase started earlier in the grafted ovaries treated with rat ASCs obtained from transgenic mice that overexpress the green fluorescent protein (p<0.05).And they also observed that increased VEGF-A expression (11-fold in grafted ovaries and 5-fold in topic ovaries vs. control) and an increased number of blood vessels (p<0.05) in ovarian tissue without leading to apoptosis or cellular proliferation (p>0.05).In the torsion group in our study, the endometrium and myometrium vascular endothelial cells in the VEGF showed positive reaction.VEGF showed a significant increase in inflammatory cells.In detorsion group, torsion VEGF expression showed more regular distribution of the group, while in the melatonin group showed VEGFpositive reaction in the small blood vessels in the endometrium.It is an important marker on a powerful endothelial mitogen VEGF angiogenesis regulation.We suggest that VEGF are a regulator of the microvascular response of the endometrium Sapmaz-Metin et al. 45 observed apoptotic cell number increased significantly in ovaries after IR.An experimental IR study of Yigiter et al. 46 showed that the apoptotic cell number increased after ischemia but decreased significantly in a growth hormone treatment group.Ferreira et al. 47 have reported that melatonin affects the mechanism and reduces the apoptosis of the uterus of rats exposed to continuous light.After the administration of melatonin, they observed the regulation of important anti-apoptotic genes such as Api5, Aven and Bcl2.In according to their result, these genes support the results of DNA fragmentation by the TUNNEL analysis method and they suggested that melatonin influences the mechanism of apoptosis.In our study, apoptosis was evaluated by Bcl2 and Western blot analysis.In the torsion group, apoptotic cells increased in the uterine epithelium.In the melatonin group showed that the Bcl2 negative effect on the uterine epithelium and did not lead to apoptotic cells.Melatonin inhibition of cellular apoptosis by ischemia/reperfusion has been an effect on the uterine tissue.
In the torsion+detorsion group, significant damage was observed in endothelial cell damage in the deteriorating vessel wall.It was also found that basal membrane structure deteriorated and inflammatory cell infiltration increased.It was found that the basal membrane structure was organized in the melatonin-treated group, the endothelial cells were composed of the normal appearance and the flat nuclei.As shown in the immunohistochemical and Western blot analyses, an increase in VEGF expression resulted in the rearrangement of endothelial cell growth and the induction of angiogenesis.Apoptotic changes were decreased in epithelial cells in endometrium, mitosis was increased in cells, Bcl-2 expression was negative.Bcl-2 expression was found to be positive in some uterinal glands located near the myometrium of the uterus.Depending on the effect of melatonin, apoptotic changes were observed in the cells of the surface epithelium, but apoptosis did not decrease with the positive expression of Bcl-2 in the uterine glands.

■ Conclusion
Melatonin-bearing receptors are induced by exposure of cells in uterine endometrial and myometrial layers to melatonin administration due to torsion and detorsion model.But, it was thought that the application of melatonin in the torsion-torsion models did not show the same characteristics in each layer of the uterus in terms of apoptosis and VEGF, Bcl-2 expressions in estrus phase.

Figure 1 -
Figure 1 -The expression of Bcl-2 and VEGF on uterine tissue was seen in estrus phase.Equal amounts of total proteins were run on the gel and analysed by Western Blotting using anti-Bcl-2, anti-VEGF and anti-β-actin antibodies.β-actin was used as a loading control (n=4).

Figure 2 -
Figure 2 -Histogram showing the length of the uterine luminal epithelium and diameter of uterine glands in all groups.

Figure 3 -
Figure 3 -a.Control Group.Normal appearance of the uterine mucosa HE staining, Bar 100µm.b.Torsion Group.Uterine epithelial desquamation (red arrow), an increase in inflammatory cells in the lamina propria, vascular dilatation and hemorrhage, degeneration and apoptotic cells in the uterine glands (yellow arrow), HE staining, Bar 100µm.c.Torsion+Detorsion Group.Uterine epithelial desquamation (yellow arrow), lamina propria inflammation and edema (blue arrow, hemorrhage, vascular degeneration and apoptotic cells in the uterine glands (green arrow), HE staining, Bar 100µm.d.Torsion+Detorsion+Melatonin Group.Regular cylindrical epithelium (red arrow), mild hemorrhage in small vessels of lamina propria (yellow arrow), in view close to normal cells in the uterine glands, HE staining.Bar 100µm.

Table 1 -
MDA concentrations in all groups.

Table 2 -
The length of the uterine luminal epithelium.

Table 3 -
The diameter of the uterine glands.