Age-related changes in the Brazilian woman ’ s smile

The aim of this research was to evaluate age-related changes in the smile of Brazilian women. The sample consisted of 249 Brazilian women who had not undergone previous orthodontic treatment or facial surgery. They were divided into four groups, according to age: G1 (20-29), G2 (30-39), G3 (40-49) and G4 (50 or older). Standardized front view photographs were taken while smiling and at rest. Measurements were evaluated by ANOVA and post-hoc Tukey. The Chi-square test was applied for qualitative variables. Upper lip thickness at rest and exposure of upper incisors on smiling decreased with age. Most individuals (60.9%) exhibited a medium smile. High smiles were more often seen in G1 (45%) and less frequently in G4 (18.8%), whereas the opposite occurred with the low smile, i.e., G4 (21.9%) and G1 (6.7%). Variations among the groups were observed in the transverse exposure of the teeth on smiling. In G1 and G3, there was a balance between tooth exposures, so that the teeth were exposed as far as the premolars and/or molars. Most of the women (56.3%) in G2 exposed their teeth as far as the first molars on smiling, whereas most of those (40.6%) in G4 exposed their teeth only as far as the first premolars on smiling. As age increased, there was decreased exposure of the upper incisors, decreased upper lip thickness and lower exposure of teeth vertically and transversely.


Introduction
Encouraged by advances in technology and influenced by the media, people are increasingly seeking a good appearance, especially adult patients. 1The mouth and the teeth are the key components of facial beauty, and, just like all other body tissues, they change with age.Certain precautions should be adopted to preserve the youthfulness of a smile when planning and performing aesthetic treatment.
Smile aesthetics depends on the interaction of structures such as lips, teeth and cheeks.Professionals should focus their attention on the morphology, length, width, volume and exposure of the lips. 2,3he amount of incisor exposure when the lips are at rest or smiling can influence the perception of facial attractiveness, and should be part of any orthodontic evaluation. 4,5However, this is not a static relationship.Incisor exposure at rest is closely related to age, caused by decreasing muscle function over the years, which increases the length of the upper lip, consequently decreasing upper incisor exposure. 6,7Other factors such as intercommissural distance and interlabial distance should be also considered, since they may affect the smile throughout life. 8,9he literature reports different smile classification methods.One method involves the degree of awareness.Thus, a smile may be posed or voluntary, if not involving emotion, and, in this case, is easily reproducible.It may also be involuntary, when induced by pleasure; in this case, it is dynamic and cannot be sustained for a very long time. 8rom an anatomical point of view, a smile may be classified as high, medium or low, according to the location of the gingiva and the relationship between the lower border of the upper lip and the upper incisal edges. 8The ideal smile arc and the parallel smile arc are also important components, usually seen in younger patients. 10,11,12However, if the upper incisal margins are straight, the smile is called a straight smile, and if the incisal margins form a reverse curve, it is called a reverse smile. 13,14,15uring diagnosis and planning for treatment procedures, orthodontists should evaluate the facial hard and soft tissues in three dimensions, sagittal, vertical and transversal.A fourth dimension warranting evaluation is the time factor. 7he aim of this study was to assess some of the changes that have been occurring in the smile of Brazilian women over the years.Identification of the features likely to change as faces age is essential to preserving a youthful smile when setting treatment goals for adult patients.

Methododology
The sample consisted of 249 adult Brazilian women, randomly selected from the general population.All individuals who agreed to participate in this study signed an informed consent form that explained the research objectives.The research project was approved by the Research Ethics Committee of the Universidade Sagrado Coração -USC, under protocol number 118/11.
Inclusion criteria included women no younger than 20 years of age, no skeletal discrepancy, and willingness to participate in the study.Exclusion criteria were individuals with missing teeth, previous orthodontic treatment and/or facial surgery, and facial asymmetry.
The sample (minimum age of 20 and maximum age of 69) was divided into 4 groups according to age: G1 (20-29 years), G2 (30-39 years), G3 (40-49 years), and G4 (50-69 years) (Table 1).In regard to the sample size, at least 30 individuals were required to achieve a 0.80 effect size with a power of 80% among the age groups (significance level set at 5%).
Two photographs were taken of each individual in front view, during the posed (voluntary) smile and at rest.The posed smile is voluntary and static, in the sense that it can be sustained.In orthodontic smile analysis, the posed smile is commonly evaluated because it is reproducible. 8hotographs were acquired using a Canon EOS Rebel XTi digital camera with Macro 100/35 mm lens and a Canon circular flash.The camera was adjusted to an exposure time of 1/60 second and aperture of 5.6.
A black #5 string with a weight on one end was hung from the ceiling and served as a plumb line, simulating the true vertical line.Thus, throughout the snapshots, the patient's head was positioned parallel to this line (Figure 1).The subjects remained in a standing position, at a distance of 1.27 m from the camera.The individuals from the sample were asked to keep their teeth in occlusion for the rest capture, spontaneously revealing a competent or incompetent lip seal.The posed smile is voluntary and need not be elicited or accompanied by emotion; it was obtained asking the patient to smile as for school picture photographs 8 (Figures 2, 3, 4 and 5).
The camera was screwed onto a professional Vivitar tripod with vertical and horizontal leveling.A mirror was placed behind the camera to induce the individual to look directly at her reflected image, in order to determine her natural head position (NHP) 16 (Figure 1).All measurements were performed with Radiocef Studio 2 software.
The following structures were measured at rest: (1) upper lip length, distance from the subnasal point to the stomion, (2) upper lip thickness, vertical distance from the uppermost point of the cupid's bow on the midline and (3) intercommissural width (Figure 6).The following structures were evaluated on smiling: (1) upper lip length, (2) upper lip thickness, (3) exposure of upper incisors, from the stomion superius to the incisal edge of the upper incisor, (4) interlabial gap, distance between the upper and lower stomion, (5) outer intercommissural width, (6) distance between the incisal edge of the upper incisors and the lower lip, (Figure 7), ( 7) type of smile, rated as high, medium or low, 17 (8) smile arc, rated as straight, reverse or parallel, (9) transverse exposure of teeth, showing the most posterior teeth exposed on smiling.

Statistical Analysis
The data were described by means and standard deviations.In examining reliability, the same examiner (LNALC) measured the first 50 subjects at two periods (1 month apart).Accordingly to the Pearson correlation test, the intraexaminer error was highly acceptable, i.e., from 0.86 to 0.98.
Analysis of variance with one criterion and the post-hoc Tukey test were used to compare the groups using quantitative measurements.The Chi-square test was used to compare the groups using qualitative variables.A level of significance of 5% (p < 0.05) was adopted for all the tests.All statistical procedures were performed with Statistica version 5.1 software (StatSoft Inc., Tulsa, USA).

Results
The study results are shown in Tables 2-6.The upper lip length at rest tended to increase with age; however, no significant differences were found among the groups (p > 0.05) (Table 2).
A decrease in the upper lip thickness at rest was found.Post-hoc analyses showed a significant difference in upper lip thickness between G1 and G4, and between G2 and G4 (Table 2).
At rest, the intercommissural distance tended to increase from G1 to G2 and from G3 to G4.However, this change was not statistically significant (Table 2).
Interlabial gap while smiling increased when comparing G1 with G2.Furthermore, there was a decrease from G2 to G3 and to G4.However, no statistically significant differences were noted across the groups (p = 0.362) (Table 3).
Regarding the exposure of upper incisors, a statistically significant decrease (p = 0.034) was observed among the groups.However, this finding was statistically significant only when comparing G1 to G4 (Table 3).
When assessing a variable called upper incisor edge, no statistically significant differences were found among the groups (p = 0.411).The upper lip length showed a progressive increase, although this difference was not statistically significant (p = 0.769) (Table 3).
On smiling, the upper lip thickness varied and the outer intercommissural distance widened, among the groups, both results having no significance (p = 0.491 and p = 0.636, respectively) (Table 3).
In relation to the smile arc, most individuals showed parallel arcs.G3 presented the highest percentage of individuals with a straight smile arc, and G2, with a harmonious smile arc; however, no statistical differences were found (Table 4).
The highest percentage of subjects evaluated had a medium smile.Most of the individuals who had a high smile were in G1 (45%), and the least number were in G4 (18.8%) (Table 5).Assessing the transverse exposure of teeth on smiling, G1 had only 3.3% of subjects with exposed upper canines, whereas the exposure of first premolars, second premolars and first molars was balanced among the subjects in this group.(Table 6).

Discussion
The smile is the best known facial expression of the human being, and every individual has the innate ability to recognize its beauty. 7,8,9,18,19,20Improving one's smile is the key purpose compelling patients to seek orthodontic treatment.Since the aging process can change the characteristics of the smile, this process should be evaluated to provide adequate treatment planning for adult patients.
In this study, the parameters of Brazilian women's faces were measured to evaluate whether or not aging would influence the smile.Since the sample comprised only women, gender differences were not considered in this study.
Regarding the length of the upper lip at rest (Table 1), an increase of 0.24 mm from the youngest to the oldest group was observed, but it was not statistically significant (p > 0.05).These results corroborate those by Formby et al., 21 (0.83 mm from 18 to 42 years old), and by Desai et al., 9 who noted an increase of 1.11 mm in the older group vs. the younger group, also with no statistical significance.Upper lip length on smiling increased progressively with age (Table 3), although this difference was not statistically significant (p = 0.769).Similar results showed that lip length tends to increase with age. 9oreover, aging leads to loss of muscle tone and increasingly greater lip sagging.This probably increases the length of the lips, causing less exposure of the upper anterior teeth.
The relationship between youth, beautiful faces and plumper lips has been highlighted by the media.A significant decrease in upper lip thickness at rest was found among the groups (p = 0.020).However these differences were not significant among G1, G2 and G3, or between G3 and G4.Post hoc analysis showed that upper lip thickness decreased by a significant 1.15 mm between G1 and G4, and 1.09 mm between G2 and G4.A similar finding on lip thinning between young and older groups was also reported. 9hese results lend further credibility to the empirical observation that older people develop thinner lips.The literature reports that the intrinsic fibers of the labial muscles, responsible for maintaining upper lip thickness, are affected by age. 21This measurement is different when individuals with a voluntary smile were assessed (Table 2).The data regarding upper lip thickness on smiling showed an increase among G1, G2 and G3, and a decrease only for G4.However the   differences among the groups were not statistically significant (p = 0.491).
Initially, the goal was to evaluate changes in the intercommissural distance in relation to perioral muscles affected by age.As the skin loses volume and elasticity, it can contribute to increasing the wrinkles at the corners of the mouth, thus masking comissures. 22onetheless, the intercommissural distance was measured at rest and showed an increasing trend from G1 to G2 and from G3 to G4, but this change was not statistically significant across the groups.Similar findings reported an increase of 2.71 mm. 9 On the other hand, the outer intercommissural distance on smiling increased among the groups, with no significant difference (p = 0.636) (Table 3).
The interlabial gap on smiling tended to increase with age (Table 3), but this was not considered a statistically significant finding.These results do not support those that reported a significant age-related decrease in interlabial gap height, a widening of the smile in the transverse direction and a narrowing of the smile in the vertical direction. 9he literature emphasizes that the upper incisor display is directly related to age. 4,6,7,8,9,13,21,22,23,24,25,26,27,28,29his feature is important not only for the aesthetics of the smile, but also for the attractiveness of the face. 25Regarding the exposure of upper incisors while smiling (Table 2), a statistically significant decrease was found (p = 0.034).However, a decrease in this variable was statistically significant only when Group 1 was compared with Group 4 (1.86 mm).Vig and Brundo 13 reported similar results; they noted a decrease of approximately 3.41 mm in the incisor exposure in younger patients (29 years old) compared with those over 60 years of age.Dong et al. 26 described a 2 mm reduction in the exposure of upper incisors between the groups aged 20-29 years and 60-69 years.These results are also consistent with previous studies, 6,13,24,25,26,27 thus confirming that young people expose their incisors more prominently when smiling than older people do.These changes are not determined by teeth position variation but rather by the impact of aging on facial tissues, and the effect of gravity on the lips. 24,28These data are of utmost importance for orthodontic diagnosis and planning, in order to fulfill patient expectations regarding the youthfulness of the smile in the long term.
Changes in the distance from the upper incisor edge to the lower lip were not statistically significant among the groups (p = 0.411) (Table 3).There was an increase in this distance from G1 to G4.Although not statistically significant this finding conflicts with that of other studies. 9,17,26In the latter, the authors curiously suggested that people become more aware of their smile as they mature, and shy away from showing their teeth if they do not like how they look, by covering the incisal edges with the lower lip.
In this study, smile arc assessment showed that most of the individuals in all the groups displayed parallel or harmonious arcs (Table 4).This observation agrees with that of other authors 9,17,26 who evaluated the smile arc, except for Maulik and Nanda, 29 who reported a straight smile in most subjects (49%).The percentage of subjects with reverse smile arcs was lower in all the groups; this finding corroborates other studies (3.6%, 9 1.32%, 17 and 5% 26 ).Although bearing no statistical significance, the highest percentage of harmonious smiles was found in G2 (56.6%); this finding is in agreement with other studies, and shows that young patients usually have parallel arcs. 2,10,29n relation to the gum line, the smile can be classified as high (100% of upper incisors plus gingiva are exposed), medium (75 to 100% of upper incisors are exposed) or low (less than 75% of upper incisors are exposed). 13,17,19,29This information is of great clinical relevance, since a high smile undergoes a certain degree of spontaneous correction over time. 9In general, the highest percentage of subjects evaluated exhibited a medium smile (60.9%).These data are consistent with those of other authors, who found 73.8%, 9 56.9%, 29 68.9% 17 and 56%. 26Most individuals with a high smile were from G1 (45%), whereas the least were from G4 (18.8%).These findings suggest that the upper lips increasingly cover the upper incisors with age.
In assessing the transverse exposure of the teeth on smiling, it was noted that only 3.3% of the subjects in G1 exposed their upper canines, whereas the exposure of first premolars, second premolars and first molars was balanced.Most of the individuals from G2 (56.3%) exposed their teeth as far as the first molars on smiling.In G3 there was a balance between the exposures of the different teeth, that is, the subjects exposed their teeth as far as the premolars and/or molars.Most of the individuals in G4 (52.1%) exposed their teeth only as far as the first premolars on smiling.When investigating changes in the intercommissural distance, a decrease was found that could explain the exposure of lower teeth in older women.It is also speculated that changes in muscle tone may occur; this could interfere in the ranges of this distance.Dental casts plus photographs are strongly advised in order to obtain more accurate assessments.This procedure would allow more reliable conclusions to be drawn regarding this specific feature of the smile.This is important, bearing in mind that the aging process affects the perioral tissues and, consequently, the smile.The differences in sociocultural backgrounds and racial origin traits support the importance to study the age-related changes in different populations.
The present study showed that changes do occur as Brazilian women age; thus, a better understanding of this process and an individual approach are necessary to provide cost-effective treatment.The limitation of this study was the static protocol of the smile evaluation.An ideal situation would be to capture and analyze the smile with videography and computer software, as reported in other studies.The advantage of the smile evaluation conducted in this study regards the picture captured from digital videos of the best snapshots. 9,12

Conclusions
The major changes observed in the smile of aging Brazilian women were: a. Reduced upper lip thickness at rest -from G2 to G4, respectively (0.6%, 4.7%, 11.8%) b.Reduced exposure of the upper incisors on smiling -from G2 to G4, respectively (4.7%, 6.3%, 13.3%).c.Less exposure of the teeth in the transverse and vertical directions.

Figure 1 . 27 .
Figure 1.27.Patient positioned in front of light box with camera placed on the tripod.

Figure 6 .
Figure 6.Measurements taken at rest.Figure 7. Measurements taken on smiling.

Figure 7 .
Figure 6.Measurements taken at rest.Figure 7. Measurements taken on smiling.

Table 1 .
Description of the sample (age groups).

Table 3 .
Measurement results obtained by age group on smiling -Mean (M), Standard Deviation (SD), p-value (p).
*Statistically significant difference (p < 0.05).Groups with the same letter do not differ significantly.

Table 2 .
Measurement results obtained by the group at rest, Mean (M), Standard Deviation (SD), p-value (p).

Table 4 .
Percentages of arc types by group.

Table 6 .
Percentages of the transverse smile by group.p =0.004 (significant).