Improvement of physical and sensory properties of whipping cream by replacing sucrose with rebaudioside A , isomalt and maltodextrin

Cream is milk fat that contains globules that are protected by a membrane. Ot is an oil-in-water emulsion with a complexity different from that of other emulsions because the surface of the fat globules is covered by a lipoprotein membrane (Noda & Shiinoki, 1986; Wasan, 2000). Ot usually contains about 35% fat (Davidson, 2014). Nowadays, it is prepared by centrifugation followed by heat treatment to be used for food products such as coffee, tea, bread and cake (Matsumiya et al., 2017).


Introduction
Cream is milk fat that contains globules that are protected by a membrane.Ot is an oil-in-water emulsion with a complexity different from that of other emulsions because the surface of the fat globules is covered by a lipoprotein membrane (Noda & Shiinoki, 1986;Wasan, 2000).Ot usually contains about 35% fat (Davidson, 2014).Nowadays, it is prepared by centrifugation followed by heat treatment to be used for food products such as coffee, tea, bread and cake (Matsumiya et al., 2017).
Whipping cream that contains high levels of fat (35%-40%) is distributed in sweetened or unsweetened form in markets (Rhothwell, 1983).Whipping cream is used in desserts, pastries, cakes, ice cream and some types of coffee (Camacho et al., 1998;Sajedi et al., 2014).This product is a complex emulsion-based foam structure in which partially coalesced fat droplets stabilize air bubbles at the air-water interface (Sajedi et al., 2014).Whipping cream contains high levels of fat and sucrose.Oranian National Standard 13653 permits manufacturers to add up to 25% sucrose to whipping cream (Onstitute of Standards and Ondustrial Research of Oran, 2013).
Sweeteners are used in many food products as sucrose substitutes and a variety of non-caloric sweeteners are in use.These sweeteners provide a high level of sweetness in low or very low amounts.Ot is similar to the sweetness of sucrose but is not absorbed by the body and does not the have the risks associated with sucrose, such as obesity, tooth decay and diabetes (D'Brien-Nabors, 2011).
Stevia rebaudiana is a variety of wild chrysanthemum (Smith, 1991) that originates in the Rio Monday valley of northern Paraguay.Stevia is natural strong and non-nutritive sweetener that is distributed in the form of a colorless, odorless, crystalline powder (Kroyer, 2010).Ot has a suitable taste that is similar to sucrose without an aftertaste, but is 250-300 times sweeter (Belitz et al., 2009).Ot is stable during processing and storage and, in solid form, at high temperatures (up to 120°C) for one hour (Kroyer, 2010).Stevia has no effect on the blood sugar (Glucose) levels of humans with diabetes.Ot does not cause neurological complications or kidney disease.Ot has been shown to have anti-fungal and anti-bacterial effects (Goyal et al., 2010).There is no risk to teeth after consumption of stevia (Kinghorn, 2001).The results of animal studies have shown that stevia does not cause mutagenic damage or have genotoxic effects (D'Brien-Nabors, 2011).
Rebaudioside A is a glycoside from stevia with a diterpene structure.Ots chemical formula is C 44 H 70 D 23, its molecular weight is 967.013g/mol and its pure form is a white powder with a melting point of 242-244 °C and an optical rotation is of -33°.Rebaudioside A shows very good resistance to heat can withstand the pasteurization and sterilization temperatures of food products, particularly dairy products.On dairy products that are intended to be sweetened with sweetener, it is one of the best options (Mizutani, 2000).
Osomalt is a sweet, low-calorie bulking agent with properties and characteristics similar to sucrose.Ot is a sugar alcohol that is odorless, crystalline, and non-hygroscopic.Ot is a non-reducing sugar and optically active.Unlike sucrose, however, it is extremely stable with respect to chemical and enzymatic hydrolysis.Ot cannot be fermented by a large number of yeasts and other microorganisms found in nature.The sweetening power of isomalt lies between 0.45 and 0.60 as compared with that of sucrose (1.0).Osomalt has a pure sweet taste that is similar to sucrose without an aftertaste.Several sugar alcohols used as sugar substitutes have a high negative heat in solution.Although this mouth-cooling effect is a desirable feature for peppermint or menthol products, it is considered atypical in many other products.Dnly about 50% of isomalt converts into available energy; thus, it is suitable for controlling diabetes.As sugar alcohols have low digestibility, consumption of large amounts may result in a laxative effect.For certain applications, special types of isomalt have been developed.These include isomalt-ST, isomalt-HB, isomalt-GS, isomalt-DC and isomalt-LM.Osomalt-ST is the best type known.Extensive toxicological and metabolic studies have been conducted that prove conclusively the safety of isomalt (D'Brien-Nabors, 2011).
Maltodextrin is a modified starch derivative and non-sweet saccharide polymer with a dextrose equivalent (DE) of between 3 and 20.Ot has very little or no sweetness and is bland with no starchy flavor to mask other flavors.Maltodextrin is a fully-soluble carbohydrate of low bulk density.Ot can be metabolized in a way similar to starch and its application is suitable for diabetics.Two types of maltodextrin are in commercial use and have DEs of 10-14 and 15-19.The use of maltodextrin is widespread in the food industry because it can perform multifaceted functions in food systems.A wide range of such products is available in powder and syrup form.Ots properties and the low cost of maltodextrin make it ideally suitable to be used in food.Maltodextrin has a suitably creamy feel and texture in the mouth and can hold water in foodstuffs very well.Ot is possible to reach the desired sweetness by using maltodextrin in combination one or more sweeteners or sugars (Kennedy et al., 1995).On creams with high levels of fat and in whipping cream, maltodextrin can be used to reduce the fat content.Moreover, maltodextrin causes gelation, water blocking and maintains the quality of whipping cream.Ot is an enhancing agent for dry matter and viscosity in cream with a high fat content and whipping cream (Sepenja et al., 2010).
Studies have investigated the use of some sweeteners as sugar substitutes in food products.Aidoo et al. (2015) investigated the effect of the use of stevia, tomatin, inulin and polydextrose on the characteristics of sugar-free chocolate.They reported that the rheological properties of sugar-free chocolates were similar to those of the control sample.Moreover, Dzdemir et al. (2015) investigated the use of stevia and cocoa powder in ice cream.They found that a sample containing stevia and cocoa powder had the highest viscosity and overrun.The purpose of the current study was to replace sucrose with rebaudioside A, isomalt and maltodextrin in whipping cream and investigate its qualitative characteristics.

Producing whipping cream
The fat content of the whipping cream samples first was adjusted to 35% by adding skimmed milk according to the Pearson square ration formulation.Next, the cream was poured into a stainless steel container, placed in a bain-marie and heated to a temperature of 50°C.The commercial stabilizer was added first, then the rebaudioside A, isomalt and maltodextrin in accordance with the relative sweetness of the sucrose.The control sample contained only sucrose (crystallized sugar; Livi; Oran).The samples were stirred by mixer in the stainless steel container (Sumber; Ondonesia).The containers then were removed and cooled to 10 °C.Finally, 2 kg of each sample was poured into plastic containers having a capacity of 2.5 kg and stored at 5 °C for 24 h.The treatments are shown in Table 1.

Physicochemical analysis
The dry matter was determined using a moisture analyzer (Sartorius; Germany) by carefully spreading 3 g of sample onto aluminum foil until it was covered and placing it in the moisture analyzer.After the water evaporated, the value was shown on the moisture analyzer (Nielsen, 2010).

Sensory evaluation
Eight trained panelists evaluated the sensory properties (flavor, texture and overall acceptability) according to a 9-point hedonic scale.

Statistical analysis
Testing was conducted using a completely randomized design.ANDVA and Duncan's multiple range test were used for analysis of data.Data analysis was carried out using SPSS 19 software.

Physicochemical characteristics
Table 2 shows that significant differences (p≤ 0.05) were observed between the dry matter samples at 1, 30 and 60 days after production.Dn all days, the highest percentages of dry matter were found in samples 1 (control), 2 (50% rebaudioside A + 50% isomalt), 3 (50% rebaudioside A + 50% maltodextrin) and 4 (50% rebaudioside A + 25% isomalt + 25% maltodextrin).The lowest percentages of dry matter were found in samples 5 (60% rebaudioside A + 40% isomalt), 6 (60% rebaudioside A + 40% maltodextrin) and 7 (60% rebaudioside A +20% isomalt + 20% maltodextrin).Decreasing the isomalt and maltodextrin contents in other samples caused a significant difference (p≤ 0.05) because the amount of cream in all samples was similar.The complete removal of sucrose from the product and its replacement with isomalt created an acceptable percentage of dry matter (D'Brien-Nabors, 2011).The addition of maltodextrin increased the dry matter of the product, which is consistent with the findings of Kennedy et al. (1995).The results were in accordance with Oranian National Standard No.13653 (Onstitute of Standards and Ondustrial Research of Oran, 2013).
Table 3 shows that significant differences (p ≤ 0.05) were observed in the level of syneresis of the samples.During storage, syneresis decreased significantly (p ≤ 0.05).Dn all days, the highest level of syneresis was recorded for sample 2 (50% rebaudioside A + 50% isomalt).Dn day 1, the lowest level of syneresis was recorded for sample 1 (control).Dn days 30 and 60, sample 7 (60% rebaudioside A + 20% isomalt + 20% maltodextrin) had the lowest syneresis level.The syneresis of samples was found to be inversely proportional to the amount of maltodextrin.This agrees with the findings of Kennedy et al. (1995) and Sepenja et al. (2010).Syneresis indicates breakage of emulsion in whipping cream and is very closely related to viscosity.After stirring and transition of proteins in the serum phase, the viscosity increased, destroyed the elastic texture and created a viscose texture and syneresis decreased (Dinkson & Starnsby, 1987).

Sensory characteristics
Figure 1 indicates that significant differences (p ≤ 0.05) were observed in the flavor scores of the samples.The highest flavor score was recorded for sample 5 (60% rebaudioside A + 40% isomalt) and the lowest for samples 3 (50% rebaudioside A + 50% maltodextrin) and 6 (60% rebaudioside A + 40% maltodextrin).The samples containing isomalt and rebaudioside A received higher scores than those containing rebaudioside A + maltodextrin because   The different small letters show the significant difference in each column (p≤0.05);The different capital letters show the significant difference in each row (p≤0.05).The different capital letters show the significant difference in each row (p ≤ 0.05).the decrease in sweetness of the product was masked by the maltodextrin.This agrees with the findings of Kennedy et al. (1995) andD'Brien-Nabors (2011).
Figure 2 shows that significant differences (p ≤ 0.05) were observed in the texture scores of the samples.The highest texture score was recorded by sample 7 (60% rebaudioside A + 20% isomalt + 20% maltodextrin) and the lowest by sample 1 (control).Samples containing isomalt or maltodextrin and those containing both were scored as more pleasing by the panelists than those after the removal of sucrose.These also exhibited a more pleasing texture in the mouth for sweetened whipping cream.The creation of a suitable texture with the use of isomalt is in accordance with the findings of D'Brien-Nabors (2011).The favorable of properties of maltodextrin were in accordance with the findings of Kennedy et al. (1995).
Figure 3 indicates that significant differences (p ≤ 0.05) were observed in the overall acceptability score of the samples.The highest overall acceptability was recorded for sample 5 (60% rebaudioside A + 40% isomalt) and the lowest for sample 3 (50% rebaudioside A + 50% maltodextrin).Samples containing rebaudioside A + isomalt and those containing rebaudioside A + isomalt + maltodextrin were more desirable than those containing rebaudioside A + maltodextrin and received higher scores.This agrees with the findings of D'Brien-Nabors (2011).

Conclusion
Replacing the sucrose in whipping cream with rebaudioside A, isomalt and maltodextrin improved its physical and sensory properties.The addition of maltodextrin increased the viscosity and decreased syneresis, which improved the texture, an important factor for both industry and the consumer.The use of rebaudioside A and isomalt gave the product a sweet taste similar to that of  sucrose, or even better.During replacement of sucrose with rebaudioside A, isomalt and maltodextrin, special attention should be paid to the balance of sweeteners and maltodextrin to assure that the characteristics of the product approximate the amount of sucrose and its sweetness.The small amounts of rebaudioside A needed and nominal price of isomalt and maltodextrin make their use in whipping cream cost efficient.This product is healthy and safe.

Figure 1 .
Figure 1.The flavor score of whipping cream samples during storage.

Figure 2 .
Figure 2. The texture score of whipping cream samples during storage.

Figure 3 .
Figure 3.The overall acceptability score of whipping cream samples during storage.

Table 1 .
The treatments of the study.

Table 2 .
The dry matter (%) values of whipping cream samples during storage (mean ± SD).The different small letters show the significant difference in each column (p ≤ 0.05); The different capital letters show the significant difference in each row (p ≤ 0.05).

Table 3 .
The amounts of syneresis (%) of whipping cream samples during storage (mean ± SD).

Table 4 .
The viscosity (cp) values of whipping cream samples during storage (mean ± SD).