Tomato growth analysis across three cropping systems

The objective of this work was to analyze the growth of the Upiã tomato cultivar in the Vertical, Crossed Fence and Viçosa cultivation systems, in order to obtain explanations for the productive gains achieved in the Viçosa system. The experiment was conducted in Viçosa, Minas Gerais State, Brazil, from August 21st to December 5th, 2012, in the scheme of subdivided plots, being the plots represented by the cultivation systems: Vertical, using tape, 1.0×0.5 m spacing; Crossed Fence, staked with bamboo, 1.0x0.5 m spacing; and Viçosa, using tape, 2.0x0.2 m spacing. The subplots were composed by the sampling times of the plants: 15, 30, 45, 60 and 75 days after transplanting. The experimental design was in randomized blocks, with four replications. Each plot was composed by three lines of 10 plants, making a total of 30 plants per plot, being evaluated the four central plants of each plot. We evaluated the dry matter of leaves (MSF), stem (MSC), inflorescences (MSI), fruits (MSFr) and total (MST). Using the foliar area index, measured by digital scanners and the previously obtained dry masses, we determined the physiological growth indices: foliar area index (IAF), specific foliar area (AFE), relative growth rate (TCR), and net assimilation rate (TAL). The Viçosa system altered the growth pattern of the tomato, quantified by the growth analysis, in comparison to Crossed and Vertical Fences. The prolongation of the second growth phase for the dry matter of fruits could possibly explain the productive gains obtained in the Viçosa system. Additional studies are required in order to clarify the relationship between the duration of the second phase of fruit dry matter growth, the physiological indexes AFE, IAF and TAL with the size and fruit yield of the tomato.

Received on December 18, 2015; accepted on November 7, 2016 is called Vertical, with bamboo or clips, which appeared in an attempt to increase the distribution of solar radiation in the canopy of the plant, to promote better ventilation, thereby reducing leaf wetting time, in addition to improving the efficiency of the methods of pest and disease control (Wamser et al., 2008).
Recently, the Viçosa system for tomato cultivation has been proposed (Almeida et al., 2015).This system brought together the best results from scientific research into tomato crop management and treatment.
The productive and economic efficiency of the Viçosa system was compared to that of the Vertical and Cross-Fence systems (Almeida et al., 2015), observing that the Viçosa system provided increases in productivity and production of larger fruit of up to 61 and 131%, respectively, however without affecting the taste of the fruits.In addition, the profitability was up to 223% higher than for the Crossed Fence system.
However, little or nothing is known about the physiological growth rates of tomatoes involved in the higher productivity scenario observed for the Viçosa system.In the present study, it was possible to evaluate the influence of the cultivation system on the assimilative capacity and the assimilation by the different organs of the plant (Heuvelink, 1999).
The greater productive gain achieved with the Viçosa system for tomato cultivation is still little understood today, from an ecophysiological point of view.Thus, the objective of the work was to analyze the growth of the tomato in the Vertical, Crossed Fence and Viçosa harvest systems, in order to obtain explanations for the productive gains achieved in the Viçosa system.
The distribution of fertilizers (via fertigation) and the cultural treatments carried out during the experiment followed the recommendations of Alvarenga (2004).
The Irriplus ® computational program was used to manage irrigation, by which the water demand of the tomato was determined by means of the coefficients of adjustment (crop coefficient -kc, irrigation location -kl and soil -ks) over the reference evapotranspiration (ETo), upon availability of daily information on: maximum, average and minimum temperatures ( o C), wind speed (m/s), relative humidity (%), precipitation (mm) and radiation (W/m 2 ), obtained from an automatic weather station installed in the experimental area.The total volume of precipitation and irrigation during the experiment was 323.7 and 162.9 mm, 323.7 and 159.2 mm, 323.7 and 142.2 mm, for the Viçosa, Vertical and Crossed Fence systems, respectively.The average maximum and minimum temperatures during the period were 27.1 and 14.6ºC, respectively.
The experiment was conducted in a split plot scheme, and plots were represented by the cropping systems (Figure 1): Vertical, staked with narrow ribbon and 1.0x0.5 m spacing; Crossed Fence, staked with bamboo with 1.0x0.5 m spacing; and Viçosa, staked with narrow ribbon and spacing of 2.0x0.2m(Almeida et al., 2015).The subplots were composed by the times of samplings of the plants: 15, 30, 45, 60 and 75 days after transplanting (DAT).The experimental design was a randomized block with four replications.Each plot consisted of three rows of 10 plants, with a total of 30 plants per plot.We evaluated the four main plants in each plot.
Four tomato plants were collected per treatment for each sampling period, always in the morning.The plants were fractionated into stems, leaves, inflorescences and fruits, and placed in a greenhouse with forced air circulation, at a steady temperature of 75ºC, until reaching constant mass.
The data were analyzed through analysis of variance and regression.For the qualitative factors the averages were compared using the Tukey test, adopting the level of 5% of probability.For the quantitative factors, the models were chosen according to the significance of the regression coefficients and the biological phenomenon.
For the case of non-linear regression the model used was: where: a, b and c are adjustment parameters, and e i is the error.
We also determined the minimum critical point (PC min ), which represents the moment in the accumulation curve Tomato growth analysis across three cropping systems in which expressive gains in dry matter start to occur, and the maximum critical point (PC max ), which represents the moment when the accumulation of the components begins to stabilize, as previously described (Alves et al., 2013):

RESULTS AND DISCUSSION
There was no significant effect of the harvest systems on the dry matter data, and the interaction between season and cropping systems happened only for MSF.The MSF, MSC, MSI, MSFr and MST characters showed sigmoid behavior across all the tomato growing systems (Figure 2).
In general, PC min for the MSC (35 and 33 DAT), MSI (45 and 45 DAT), MSFr (51 and 52 DAT) and MST (39 and 38 DAT) characters were similar for the Vertical and the Crossed Fence systems, respectively (Figure 2).On the other hand, PC min for MSF was anticipated in six days in the Crossed Fence system in comparison to the Vertical and Viçosa systems, which occurred at 34 DAT for both systems.In the Viçosa system, PC min was observed at 42, 48, 53 and 45 DAT for MSC, MSI, MSFr and MST, respectively.
During the period of assessment (75 DAT), it was not possible to determine PC max in the Viçosa system for dry matter.On the other hand, in the Crossed Fence system it was possible to determine PC max for MSF (57 DAT) and MSFr (70 DAT); in the Vertical system, PC max occurred at 57, 70, 72, 67 and 68 DAT for MSF, MSC, MSI, MSFr and MST, respectively (Figure 2).
Based on PC min and PC max , we were able to establish three growth phases for the evaluated characters of the tomatoes.The first phase of growth was slow with limited PC min ; the second phase of intense growth showed limits between PC min and PC max , and finally, the third phase of maturation, initiated in PC max .
The interaction between systems and times was not significant for the physiological growth characters of AFE, IAF, TAL and TCR.However, there was a significant difference between the cultivation systems specifically for the IAF at 75 DAT, when the Viçosa system was superior to the Crossed and Vertical Fence systems, the latter two having behaved very similarly to one another.
When analyzing the behavior of the growth curves, we have observed variations in growth as a response to the cultivation system for AFE, IAF, TAL and TCR (Figure 3).We have observed that while AFE showed exponential quadratic behavior, IAF was sigmoid, TAL was asymptotic in Crossed Fence and Viçosa, and cubic in Vertical systems, and TCR showed cubic behavior across all systems.
Regardless of the harvest system, AFE was reduced throughout the evaluations (Figure 3a), which was expected as AFE relates the leaf area with the leaf dry mass.Thus, selfshading increases as the plant grows, hampering leaf area expansion but without change to the patterns of leaf and total dry mass gain (Benincasa, 2003).In addition, AFE reduction was observed in plants growing under low light intensity as an adaptive mechanism to maximize available light capture and to meet photosynthetic demand (Fan et al., 2013).
PC min for IAF occurred near 30 DAT for the Crossed Fence and Viçosa systems, and later (at 36 DAT) for the Vertical systems.PC max was close to 52 DAT for Crossed and Vertical Fence systems, and at 58 DAT for the Viçosa systems (Figure 3b).The PC max values obtained for IAF are in agreement with those obtained by Fayad et al. (2001) in the same location as in this study, who observed maximum IAF at 58 days, reaching the end of the cycle at 0.17, which the authors attributed to the period of senescence and foliar abscission.The extension of the second phase for the IAF delayed the onset of senescence and foliar abscission of the tomatoes cultivated in the Viçosa system in comparison to the other cultivation systems, a fact that would explain, at 75 DAT, the highest IAF for the tomatoes cultivated in the Viçosa system.
The TAL presented similar behavior in the Crossed Fence and Viçosa systems at 75 DAT; in contrast, in the Vertical system TAL dropped sharply, representing half of the value observed in the Viçosa system (Figure 3c).It should be noted that the reductions in MST and IAF at 75 DAT may have potentiated reductions in TAL in the Vertical system, provided that they are parameters used to determine it.This was not observed for TAL in the Crossed Fence system, because it presented MST similar to the Viçosa system.In addition, PC max for MST and MSF in the Vertical system were reached at 68 and 57 DAT respectively, a fact that reinforces the behavior of TAL in this system.The decline in TAL with increasing plant age seems to be common in short-cycle plants, since TAL represents the dry matter gain per area unit within the time unit, and provides inference to the photosynthetic efficiency (Farias & Saad, 2011).
The TCR showed maximum growth across all cultivation systems, close to 38 DAT when, from then on it decreased until the end of the evaluations (Figure 3d).Decreases in TCR values throughout the cycle are common for crops such as tomatoes and peppers.
The tomatoes cultivated in the Viçosa system presented a longer second Tomato growth analysis across three cropping systems stage and consequently, by inference the maturation of the evaluated characters (PC max ) occurred later in comparison to the other systems.Thus, the fruiting period was prolonged in this particular system, which would explain the gains of up to 61 and 131% respectively, in terms of productivity and production of large tomato fruits when grown in the Viçosa system, as observed in another study (Almeida et al., 2015).
Although no significant differences have been observed, additional studies are required to elucidate evidence of the possible relationship between the greater extent of the second stage of MSFr growth of tomatoes cultivated in the Viçosa area and greater AFE, IAF and TAL during this phase in this system.In addition, one must study the environmental factors that favor this evidence.The Crossed Fence system promotes the convergence of cauline apices, which can accelerate selfshading and consequently, reduce AFE, LAI, TAL, as well as the duration of the second phase of MSFr growth and fruit size in this system in comparison to the Viçosa system.Inference could explain the higher production of average tomato fruits in the Crossed Fence system in comparison to the Viçosa system as observed by Almeida et al. (2015).
In light of the above, and according to the results obtained, it is possible to conclude that the Viçosa system altered the growth pattern of the tomatoes, quantified by the growth analysis, in comparison to the Crossed and Vertical Fence systems.The second growth phase for the dry matter of the fruit would possibly explain the productive gains obtained by Almeida et al. (2015).Further studies are required to clarify the relationship between the duration of the second phase of fruit dry matter growth, as well as the relationship between the physiological indexes AFE, LAI and TAL and the size and fruit yield of the tomatoes.