Bone biopsy in nephrology practice

Abstract Renal osteodystrophy (ROD), a group of metabolic bone diseases secondary to chronic kidney disease (CKD), still represents a great challenge to nephrologists. Its management is tailored by the type of bone lesion - of high or low turnover - that cannot be accurately predicted by serum biomarkers of bone remodeling available in daily clinical practice, mainly parathyroid hormone (PTH) and alkaline phosphatase (AP). In view of this limitation, bone biopsy followed by bone quantitative histomorphometry, the gold-standard method for the diagnosis of ROD, is still considered of paramount importance. Bone biopsy has also been recommended for evaluation of osteoporosis in the CKD setting to help physicians choose the best anti-osteoporotic drug. Importantly, bone biopsy is the sole diagnostic method capable of providing dynamic information on bone metabolism. Trabecular and cortical bones may be analyzed separately by evaluating their structural and dynamic parameters, thickness and porosity, respectively. Deposition of metals, such as aluminum and iron, on bone may also be detected. Despite of these unique characteristics, the interest on bone biopsy has declined over the last years and there are currently few centers around the world specialized on bone histomorphometry. In this review, we will discuss the bone biopsy technique, its indications, and the main information it can provide. The interest on bone biopsy should be renewed and nephrologists should be capacitated to perform it as part of their training during medical residency.

Renal osteodystrophy (ROD), a group of metabolic bone diseases secondary to chronic kidney disease (CKD), still represents a great challenge to nephrologists. Its management is tailored by the type of bone lesion -of high or low turnover -that cannot be accurately predicted by serum biomarkers of bone remodeling available in daily clinical practice, mainly parathyroid hormone (PTH) and alkaline phosphatase (AP). In view of this limitation, bone biopsy followed by bone quantitative histomorphometry, the gold-standard method for the diagnosis of ROD, is still considered of paramount importance. Bone biopsy has also been recommended for evaluation of osteoporosis in the CKD setting to help physicians choose the best anti-osteoporotic drug. Importantly, bone biopsy is the sole diagnostic method capable of providing dynamic information on bone metabolism. Trabecular and cortical bones may be analyzed separately by evaluating their structural and dynamic parameters, thickness and porosity, respectively. Deposition of metals, such as aluminum and iron, on bone may also be detected. Despite of these unique characteristics, the interest on bone biopsy has declined over the last years and there are currently few centers around the world specialized on bone histomorphometry. In this review, we will discuss the bone biopsy technique, its indications, and the main information it can provide. The interest on bone biopsy should be renewed and nephrologists should be capacitated to perform it as part of their training during medical residency.

IntRoductIon
Abnormalities in circulating parameters of mineral and bone metabolism may appear early in the course of chronic kidney disease and they have been associated to increased mortality and morbidity, and decreased quality of life. 1,2 In 2006, the term chronic kidney disease-mineral and bone disorders (CKD-MBD) was implemented by the Kidney Disease Improving Global Outcomes (KDIGO) working group 3 to refer to the systemic disorder of mineral and bone metabolism due to CKD; it is manifested by either one or a combination of: 1) abnormalities of calcium (Ca), phosphorous (P), PTH and vitamin D metabolism; 2) abnormalities of bone turnover, mineralization, volume (TMV), linear growth or strength; and 3) vascular or other soft tissue calcification. There are many studies showing that these disorders are related to adverse clinical outcomes, in particular cardiovascular disease, fractures, and mortality, reaffirming the importance of this systemic pathological process. [4][5][6][7] In 2009, KDIGO published clinical guidelines for the diagnosis, prevention, and treatment of CKD-MBD. Renal osteodystrophy (ROD), a group of metabolic bone diseases that occurs through the evolution of CKD, is part of the manifestations of CKD-MBD. The term 'renal osteodystrophy' has been limited to the histologic analysis of bone lesions, requiring the use of bone biopsy. 8,9 Bone biopsy is considered the gold-standard method for histological classification of ROD. 3,[8][9][10] It is performed in the iliac crest, after double tetracycline labeling, allowing that the different histological alterations of bone tissue that make up the ROD spectrum can be diagnosed and classified by quantitative histomorphometry. This analysis consists on the evaluation of structural and dynamic parameters, such as trabecular bone volume, bone cell numbers, bone formation, and mineralization. ROD may be classified according to TMV criteria into one of the following patterns: (i) low-turnover bone disease, which includes adynamic bone disease (ABD) and osteomalacia (OM); or (ii) high-turnover bone disease, which encompasses bone disease related to secondary hyperparathyroidism (SHPT), or osteitis fibrosa, and mixed uremic osteodystrophy (MUO). 3,11,12 Figure 1 shows the different types of ROD and their characteristics. High-turnover bone disease are exemplified in figures 1A, 1B, 1C, and 1D, and low-turnover bone disease in figures 1E, 1F, 1G, and 1H.
Another important analysis is the thickness and porosity of cortical bone. About 80% of all fractures occurs at non-vertebral sites, at regions comprising large amounts of cortical bone. In the past, trabecular bone and vertebral fractures had been the hallmarks of osteoporotic fractures, but recently the role of cortical bone in bone fragility has gained a growing interest. 5,13 In the general population, cortical lesions have been consistently associated with increased fracture risk, as is observed in post-menopausal women. Bjornerem et al 14 in a high-resolution peripheral CT study, showed that cortical porosity and thickness are the main factors determining the bone fragility that underlies non-vertebral fracture risk.
CKD is associated with higher risk of fractures, being 4x greater in these patients than in the general population, increasing mortality significantly. [15][16][17] One possible explanation is that in CKD there is a decrease in bone mass with impaired bone strength and a high risk of fall, predisposing to fractures. 18 Taking these data into account, information obtained through quantitative histomorphometric analysis of bone tissue may be help us to understand the mechanisms behind the assoication of bone abnormalities and clinical outcomes, which may be crucial to improve the manangement of bone disease. 9,19 Table 1 shows characteristics of ROD according to histomorphometric parameters. Osteoporosis might be present in any type of bone disease, with the exception of osteomalacia.
Bone biopsy is an invasive and costly procedure that requires specialized centers for histomorphometric analysis. Therefore, it has not been recommended as part of the routine evaluation of CKD-MBD. 9,13 The biomarkers of bone remodeling PTH and AP, both total and the fraction, have been considered a helpful tool for predicting ROD type. 8,20 Otherwise, some studies have reported that PTH is a poor biomarker of bone disease due to its high biological variability. 20,21 In dialysis patients, PTH levels < 100 and > 450 pg/mL have been associated with low-and high-turnover bone disease, respectively, The predictive value of PTH is not considered ideal especially when its level is between 150 -450 pg/ mL, a gray zone where any type of bone histology may occur, including normal bone. 22 Serum AP is a marker of bone formation. In general, its variation parallels that of PTH, providing additional information for predicting the type of ROD. 23,24 In some circumstances, the values of these markers may be divergent, such as high PTH and low total AP levels, which suggests the presence of SHPT associated with a low-turnover bone disease. This apparent paradox can be secondary to aluminum intoxication or over suppression of bone remodeling by active vitamin D therapy. Bone biopsy may be fundamental for clarifying this blurred scenario. 25 It is worth mentioning that bone biopsy may also provide a complementary view of bone tissue alterations, such as the detection of aluminum and/or iron intoxication and the presence of low bone volume, a finding suggestive of osteoporosis. 26,27 Bone biopsy can also provide bone tissue sample for immunohistochemical analysis, molecular studies, data on efficacy and safety of different drugs, among other advantages; [28][29][30][31] its main indications are listed in Table 2.
Finally, in view of the limitations of the serum biomarkers, other diagnostic methods have been used for the analysis of bone diseases. 32 Dual energy X-ray absorptiometry (DXA), 33 quantitative computerized tomography (QCT), 34 high resolution-peripheral QCT (HR-pQCT) 35 and micro magnetic resonance imaging (MRI) 36 are imaging methods that quantify bone mass and structural aspects of bone quality. Importantly, they do not measure neither and mineralization nor determine ROD type. Thus, currently the non-invasive assessment of bone turnover and mineralization is still suboptimal. 20,37    the costly histopathological analysis, lack of histopathological expertise, and no established reimbursement in several countries. 10 In March 2016, the European Renal Osteodystrophy (EU-ROD) initiative was created to revitalize bone biopsy as a clinically useful tool in the diagnosis of CKD-MBD to improve outcomes in CKD patients. 10 In Brazil, Oliveira RB et al created the Brazilian Registry of Bone Biopsy (REBRABO), a national registry that serves as a research platform to expand the knowledge about CKD-MBD. 38 In the next sections of article, we will overview the protocol of tetracycline labeling, and the technique to perform bone biopsy, its indications and complications. Finally, the main information provided by bone histomorphometric analysis will be discussed.

double tetRAcycline lAbeling
Patients should take tetracycline previously to the bone biopsy for an adequate evaluation of the type of bone disease. Tetracycline, a macrolide antibiotic, deposits on bone surface allowing the assessment of 1. Persistent bone pain; 2. Unexplained hypercalcemia and/or hypophosphatemia; 3. Fragility fracture; 4. Discrepancy between serum biomarkers and clinical presentation; 5. Suspicion of aluminum and/or iron intoxication; 6. Before using anti-osteoporotic drugs, such as bisphosphonates and denosumab.

tAble 3
AdvAntAges And limitAtions of diffeRent methods foR the evAluAtion of bone tissue dynamic parameters of bone formation and mineralization. The tetracycline administration scheme we have been using in our services is the following: first course of tetracycline for 3 days, 500 mg twice a day; a tetracycline-free interval of 10 days; a second course of tetracycline for 3 days, 500 mg twice a day. Bone biopsy must be performed from 3 to 5 days after the end of the second course of tetracycline. [9][10][11] bone biopsy mAteRiAl The material required for performing bone biopsy is (i) a minor surgery pack containing 1 Kelly thin straight tweezer, 1 rat tooth tweezer, 1 small bowl, 1 medium straight scissor, 1 needle holder, 1 syringe (20 mL); 1 suture thread (3.0); 1 bistoury blade; (ii) 1 set of trephine needle for bone biopsy; and (iii) 1 electric drill. Figure 2 illustrates these materials.

tRAnsiliAc bone biopsy pRoceduRe
Transiliac bone biopsy can be performed in an ambulatory surgical center in most cases. Mild sedation with midazolam, administered by intravenous or intramuscular route, should be used. Performing bone biopsy in obese patients and in children may require in-hospital surgical center and the presence of anesthesiologist.
The first step of bone biopsy procedure is to define the correct site on the iliac crest. The superior iliac crest should be located and imaginary lines drawn 2 cm below and 2 cm inwards toward the contralateral shoulder, forming a 90 o angle with the iliac bone.
Asepsis should be made before starting the procedure with, for example, alcoholic chlorhexidine. For local anesthesia, a button on the skin of around 2 cm should be made in the incision site. The anesthetic should be injected along the biopsy path and into the periosteum of the external cortical. The internal cortical bone may also be anesthetized by entering with a needle 2 cm below and posterior to the iliac crest. In the previously defined point, a 2 cm incision should be made. Thereafter, the muscle plans should be gently divulged to avoid bleeding, using a straight Kelly to make a tunnel from the skin until the periosteum. Once the tunnel is made, the Rochester Bone Biopsy kit that includes a pointed obturator (number 87410), a guide sleeve (number 87420), a drive adaptor (number 87450), a Trephine cutter (number 87401) and a blunt extractor (number 87440) ( Figure 2) will be used for the following steps: 1-The pointed obturator should be inserted through the incision until reaching the surface of the ilium to further localize the biopsy site. 2-The pointed obturator should be removed and connected to the guide sleeve. The assembly should be reintroduced into the tunnel until the previously determined site of biopsy. The guide sleeve should be held firmly while the pointed obturator is withdrawn. 3-The Trephine needle should be connected to the drive adaptor and coupled to the drill, being firmly fastened to the drill. 4-The assembly should be inserted into the guide sleeve, which acts as a catch lock. the Trephine must not exceed the guide sleeve more than 3 cm to assure that it will not excessively overpass the ilium. 5-The drill should be driven forward rotating in the clockwise direction. The Trephine needle should pass through the external cortical, trabecular, and internal cortical bone, to obtain an adequate bone sample. Thereafter, the direction of the drill rotation should be set to counterclockwise and the drill driven backwards. 6-The bone fragment should be removed from the trephine needle using the blunt extractor. 7-The incision wound should be compressed for 5 minutes to stop bleeding. 8-The incision should be cleaned and sutured using Donati technique. 9-A compressive dressing should be made to prevent bleeding. The bone fragment size should be variable, depending on factors such as gender and patient size. It is considered adequate when the fragment is composed of two cortical (internal and external) bones at the extremities and trabecular bone in the middle. Importantly, the bone specimen should not be fractured during the procedure. The sample should be stored in a vial containing 70% alcohol and protected from light exposure. Formalin should not be used because it may damage the bone tissue.

complicAtions of bone biopsy
The transiliac bone biopsy has been proved to be safe and associated with minor complications, which incidence is of 0.63%. 39,40 The most common complications are bleeding, hematoma, infection, superficial nerve injury, and pain. Routine prophylactic antibiotic administration is not recommended. Antibiotics should be reserved for the cases of excessive manipulation or accidental contamination. The main recommendations for patient after bone biopsy are listed in Table 4.

histomoRphometRic AnAlysis
The bone biopsy result is reported according to the TMV classification. Briefly, it indicates if bone turnover (T) and volume (V) are low, normal, or increased, and if the mineralization (M) is normal or abnormal. The high-turnover bone diseases comprise SHPT-related bone disease ( Figure 1A and 1B) or MUO ( Figure 1C). Both are characterized by an increase in the number and activity of bone cells (osteoblasts and osteoclasts) and, consequently, higher bone formation and resorption. (Table 1) The presence of bone marrow fibrosis is a common finding in high-turnover state ( Table 1). The main difference between these two patterns of high-turnover bone disease is the presence of abnormal mineralization in MUO (Figure 1 D; Table 1). Bone volume may be decreased in any type of ROD (excepted osteomalacia), indicating a predominance of bone resorption over bone formation (Table 1) and greater brittleness of bone tissue. Low-bone turnover disease is characterized by low cellularity (reduced number of osteoclasts and osteoclasts). Low bone formation and mineralization ( Figure 1G and 1H; Table 1) are the main finding of ABD while a greater impairment of bone mineralization, leading to the accumulation of the osteoid matrix, is the main finding of osteomalacia ( Figure 1F; Table1). Finally, it should be • The patient must take a day off • The patient should not perform heavy tasks for the following 3 days.
• The dressing should be removed 24 hours after the procedure.
• The surgical wound can be washed with soap and water.
• A new dressing or a Band-Aid may be used.
• Painkillers, such as dipyrone or paracetamol, for alleviating pain.
• Heparin should be avoided during the hemodialysis session following the bone biopsy.
• The stitches should be removed 7 -10 days after the procedure.

conclusIon
Bone biopsy is an important procedure that provides helpful information on bone microarchitecture, turnover and, ultimately, on the type of ROD. This information may be critical for choosing the most appropriate therapy for CKD-MBD and other findings as osteoporosis, aluminum and/or iron intoxication. Nephrologists should be capacitated to perform bone biopsy as part of their training during medical residency.