Sex Identification Based on Tooth Crown Trait Analysis Among the Mongoloid Race

based on tooth crown trait analysis among the Mongoloid race. Abstract Objective: To determine whether anterior and posterior tooth crown traits exhibit sexual dimorphism and identify traits characteristic to the Mongoloid race, especially among the Indonesian population. Material and Methods: This study cross-sectional study analyzed 108 dental casts from 36 males and 72 females. The traits analyzed included winging, shoveling, double shoveling, canine mesial ridge, canine distal accessory ridge, hypocone, metaconule, Carabelli’s cusp, protostylid, metaconulid, enteconulid, and hypoconulid. All tooth crown traits were scored based on the ASUDAS scoring system. Descriptive statistics were used to calculate the absolute and relative frequencies. The Chi-square tests was used to determine significant differences in anterior and posterior tooth crown traits between males and females. Level of significance was set at 5%. Results: None of the traits showed sexual dimorphism. Moreover, the most common traits among the Mongoloid race were hypocone (94.4%) and shoveling (86.1%). Conclusion: Although none of the traits exhibited sexual dimorphism, most of them had a higher incidence among females than males. Nonetheless, further research including adequate samples and a similar number of females and males, is needed, especially for population studies. The included winging (upper central incisor), shoveling (upper right central incisor), double shoveling (upper right central incisor), canine mesial ridge (upper canine), canine distal accessory ridge canine), hypocone molar), metaconule Carabelli’s protostylid tooth crown based on ASUDAS scoring system.


Introduction
Recent mass fatalities due to natural or man-made disasters still remain a considerable problem.
Moreover, such disasters have resulted in a significant number of unidentified victims, the identification of whom has become an important area of investigation. Accordingly, the identification process involves establishing biological profiles, such as age, sex, stature, and race [1,2]. Sex has generally been the first individual component to be analyzed given that other biological profiles, like age and stature, depend on sex determination. Like most other primates, humans exhibit differing morphological patterns between males and females, which is called sexual dimorphism [3].
During the past decade, considerable research has been conducted on the teeth as a means of establishing a biological profile. Human teeth have been considered one of the reliable bodily components that can be used for identifying individuals who, due to disasters or other injuries, can no longer be identified. The teeth are composed of calcified biological material that is hard, stable, and able to withstand environmental changes [8], making them suitable for forensic identification. The morphology of both anterior and posterior teeth is rather unique with some varying characteristics [9].
In fact, the Arizona State University Dental Anthropology System (ASUDAS) has described several morphological dental characteristics, a project that had been initiated by Christy Turner II in 1970 and continue by G. Richard Scott and Christian R. Nichol in 1991. The aforementioned project aimed to standardize graded guidelines that other studies worldwide can compare their scoring system [3,9]. Some of the morphological characteristics introduced by Turner and Scott include winging, shoveling, double shoveling, canine mesial ridge, canine distal accessory ridge, hypocone, metaconule, and Carabelli's cusp for anterior teeth and protostylid, metaconulid, enteconulid, and hypoconulid for posterior teeth.
Previous research involving the frequency of nonmetric traits among a Brazilian sample showed that Carabelli's cusp exhibited sexual dimorphism given is a higher incidence among males (27.1%) than females (12.3%) [3]. Another study revealed that females had significantly greater double shoveling and shoveling grades than males [10]. Besides showing sexual dimorphism, tooth crown traits can also be used as a marker for certain populations. Accordingly, shoveling has been most frequently observed among Mongoloid groups, especially Asian populations, while Carabelli's cusp has been most commonly detected among Caucasoid groups, especially European populations [11].
The present study aimed to determine whether anterior and posterior tooth morphological characteristics exhibit sexual dimorphism and identify tooth morphological characteristics among the Mongoloid race, especially in the Indonesian population.

Study Design and Sample
This cross-sectional study included 108 dental casts from 36 males and 72 females. Only dental casts with complete data, such as sex (male and female), age (12-60 years old), and race, were included. Moreover, dental casts had to include a complete set of teeth with an intact crown, especially for the upper incisors, upper canines, and upper and lower molars.

Data Collection
The dental casts were collected from the Department of Prosthodontics, Faculty of Dentistry, The measurements were carried out by three observers independently. From the dental casts that were collected for the study, 20 samples were taken for conformity testing to prove the reliability and validity of the measurements. First, the observers' perceptions were synchronized to apply the same scoring system used in similarly perception, so that the scoring system used is appropriate and has the same understanding.
Then, several exercises were carried out to equalize the data collection process. The outcome was subjected to intra-and inter-observer Kappa testing which showed a good match between measurements tested.

Results
The analysis showed that all of the traits were higher among females. However, no significant difference in each trait was observed between both sexes. The trait most common among the Mongoloid race was hypocone (94.4%) followed by shoveling (86.1%), whereas the least common trait was metaconule (4.6%), followed by Carabelli's cusp (14.8%) ( Table 1).

Discussion
The present study scored anterior and posterior tooth crown traits based on guidelines established by the ASUDAS, with the breakdown of each trait presented in the following paragraphs.
Although no sexual dimorphism has been observed for winging, its incidence was higher among females than males. Among the study population, 35.2% exhibit winging. It was revealed moderate frequencies of winging among the East and Central Asian, American Arctic, and Sunda-Pacific populations [12], with the Northeast Siberian and North and South American populations having the highest frequencies of winging [13].
The present study found no sexual dimorphism for shoveling, althea its incidence was higher among females (66.7%) than males (33.3%). Similarly, previous studies on shoveling have shown no sexual dimorphism for this trait [12]. Research carried out with Asian individuals revealed that females had a significantly greater shoveling grade than males [10], a finding consistent with that presented herein where females had higher incidences of shoveling than males. Moreover, the frequency of shoveling among the Indonesian population had been found to be rather high (86.1%) [13] published the Asian population dental complex termed "Mongoloid Dental Complex." Accordingly, one characteristic of this complex was the high frequency of shovel-shaped upper incisors [14].
Although no sexual dimorphism had been found for double shoveling, its incidence was higher among females (63.2%) than males (36.8%). A previous study revealed that females had a significantly greater double shoveling grade than males [10]. Meanwhile, Turner who studied the broad Mongoloid dental complex, showed that double shoveling was more frequent than single shoveling in the Sinodont dental complex (one division of the Mongoloid dental complex) [14].
The Mongoloid race had been found to have a low frequency of canine mesial ridge (Bushmen Canine) (28.7%), a trait first observed in 1975. This condition occurred when a strongly developed mesiolingual marginal ridge of the upper canine fused with the tuberculum dentale. The term "Bushman canine" was established due to its high occurrence among the Bushmen and other Sub-Saharan African groups, suggesting that this is one of the characteristic traits of the Negroid race [13].
The present study found no sexual dimorphism for canine distal accessory ridge, a result inconsistent with that revealed by previous author [12]. Canine distal accessory ridge was the most sexually dimorphic crown trait, with males exhibiting higher expression and frequencies [13]. Similarly, although the present study found no sexual dimorphism for Carabelli's cusp, the Brazilian study's found that Carabelli's cusp did show sexual dimorphism [3]. However, Hanihara observed no significant sexual dimorphism for Carabelli's cusp in either the frequency or expression of the structure [3].

Conclusion
Although none of the traits exhibited sexual dimorphism, most of them had a higher incidence among females than males. Nonetheless, further research, including adequate samples and a similar number of females and males, is needed, especially for population studies.