Wernicke ’ s encephalopathy in a patient with non-Hodgkin ’ s lymphoma post-Autologous HSCT

1. Doctor, Residence in Hematology, Bone Marrow Transplantation Service at Walter Cantidio University Hospital – Fortaleza (CE), Brasil. 2. Ph.D. in Pharmacy, Professor of Hematology, Federal University of Ceará – Fortaleza (CE), Brasil. 3. Ph.D. in Pharmaceutical Sciences, Federal University of Ceará Fortaleza (CE), Brasil. 4. Doctor, Residency in Radiology and Diagnostic Imaging of Walter Cantídio University Hospital Fortaleza (CE), Brasil. 5. Ph.D. in Surgery, Chief of Bone Marrow Transplantation Service at Walter Cantidio University Hospital – Fortaleza (CE), Brasil.

Wernicke's encephalopathy (WE) is an acute neuropsychiatric syndrome caused by thiamine deficiency and is associated with significant morbidity and mortality 1 .WE is a rare condition, characterized by altered mental status, as well as ocular, walking, and balance abnormalities.Although WE usually results from chronic alcohol dependence, nonalcoholic causes are reported in 20% to 50% of patients. There have been few case reports of WE in patients with malignant lymphomas 3 .The occur-rence of HSCT-related WE is also rare, mainly in autologous HSCT 4 .WE occurrence is associated with drug use, prolonged Total Parenteral Nutrition (TPN), vomiting, and malnutrition.A 36-year-old female, married, small farmer, with no history of alcoholism was diagnosed with a bulky inguinal diffuse large B-cell non-Hodgkin lymphoma IIIB -(10cm).She was admitted to the University Hospital to undergo an autologous HSCT.The mobilization was performed with Granulocyte Colony-Stimulating Factor, and 2.5 x 10 6 CD34 cells/kg

Wernick's Encephalopathy (WE) is an acute neuropsychiatric syndrome caused by thiamine deficiency post hematopoietic stem cell transplant (HSCT)
. WE is associated with high mortality and morbidity rates, but due to its rare occurrence, it is rarely considered in patients submitted to this procedure.Considering that, the manuscript reports the clinical characteristics and the possible factors that predisposed the occurrence of WE in a patient with non-Hodgkin's lymphoma post-Autologous HSCT.We conclude that WE should be considered in patients submitted to autologous HSCT associated with prolonged use of TPN and malnutrition.
were collected.The conditioning regimen for autologous TCTH was BEAC (BCNU, etoposide, cytarabine, cyclophosphamide).The infusion of hematopoietic stem cells was performed, and on D+2, she had a recurrence of fever, abdominal pain, and vomiting.On D+3 the patient's overall condition was worse, with contraction of diuresis, hypotension, and abdominal distension.Volume expansion with saline solution and vasoactive drugs were performed.On D+4, a large amount of gastric residue was observed after nasogastric intubation.On the D +5 there was an improvement of the patient's overall condition with weaning of the vasoactive drug and a gastric residue (GR) of 3650mL, followed by 1950mL on D+6, and 2,150mL on D+7.Abdominal pain and abdominal distension persisted, and TPN was introduced on D+7.On D+12 the nasogastric tube (NT) was closed, and a restricted liquid diet was allowed.She had difficulty in accepting the oral diet, and the NT was maintained.On D+18, recurrence of fever was observed, and the patient still had abdominal pain and distension and difficulty in walking.On D+22, weaning from TPN was performed, and there was an improvement in the abdominal condition.
Neutrophil grafting occurred only on D+23.On D+24 the patient partially tolerated oral diet but still required blood transfusions.She developed apathy and drowsiness.On D+30, a new febrile peak was observed, with the reintroduction of Meropenem.A clinical scenario characterized by sensorineural fluctuation, the absence of focal signs, difficulty in ambulation and horizontal nystagmus was observed.The electroencephalogram (EEG) did not show any specific findings.On D+35 cerebrospinal fluid cytology revealed negative findings for malignancy.There was no evidence of central nervous system infection.PCR tests for herpes, dengue, and chikungunya fever were negative.Magnetic resonance imaging (MRI) of the brain showed increased signal in the fluid-attenuated inversion recovery (FLAIR) sequences around the Sylvian aqueduct and in the medial parts of both the thalamus and mammillary bodies (Figures 1a and  1b).Encephalitis Protocol (Meropenem, vancomycin, sulbactam sodium / ampicillin sodium, acyclovir) was empirically introduced.Thiamine replacement was initiated with 1500mg IV for 3 days, followed by 900mg/day.Improvement in the level of consciousness and nystagmus was quickly observed, while she persisted with temporal/spatial disorientation and recent amnesia.She was afebrile at discharge with hematologic recovery.The patient continued with progressive improvement of disorientation and amnesia but had pain in the lower limbs (neuropathy).During the follow-up, neurological changes and oral ingestion gradually improved.
Neurologic complications are frequently observed in patients during HSCT, being reported in 30% to 39% of cases 5 .These complications may be infectious, cerebrovascular, toxic, immuno-mediated or metabolic 5 .The complications may be due to drugs, thiamine deficiency, among others.Wernicke's encephalopathy (WE) is an acute neuropsychiatric syndrome

FIGURE 1. AXIAL FLAIR IMAGES OF THE BRAIN DEMONSTRATING AREAS OF SIGNAL CHANGE CHARACTERISTIC OF WERNICKE'S ENCEPHALOPATHY: IN (A), SYMMETRIC HYPERINTENSITIES IN THE MAMILLARY BODIES (ARROW) AND PERIAQUEDUCTAL GRAY MATTER; IN (B), SYMMETRIC HYPERINTENSITIES IN THE MEDIAL REGIONS OF THE THALAMUS.
caused by thiamine deficiency that causes mental alterations, ocular, and balance abnormalities.Reports of WE cases in the literature associated with HSCT, mainly allogeneic transplantations, have been poorly described.Among the indicators of predisposition to WE in HSCT is prolonged total parenteral nutrition (TPN), since the latter is thiamine-deficient. 5,6Some authors have considered the prolonged use of TPN as the main risk factor for HSCT associated with Wernicke's Encephalopathy, but the duration of TPN required for the disease to manifest is unknown. 7atients receiving long-term TPN, and intravenous solutions require higher amounts of thiamine to metabolize carbohydrate intake, which may rapidly consume thiamine stocks. 3Studies show that a state of depletion can develop within 18-20 days in patients receiving a strict diet without thiamine. 5Most of the reports concluded that prolonged TPN was the primary risk factor for HSCT-associated WE. 4 Our patient with NHL underwent an autologous HSCT.Busulfan is not used in the conditioning regimen.She received TPN for approximately 2 weeks associated with episodes of vomiting.TPN includes multivitamin and mineral supplementation.The patient received prolonged TPN without thiamine, and WE symptoms appeared on day +35.Wernicke's encephalopathy was diagnosed based on the history of consistent use of TPN and CNS symptoms with symmetrical T2/FLAIR hypersignal in the hypothalamic region, mammillary bodies and walls of the 3 rd ventricle, medial region of the thalamus (Figure 1), although the level of thiamine was not assessed.
Several drugs routinely used in HSCT are associated with neurological abnormalities, including busulfan 4 cyclosporine A 5 and tacrolimus. 6We must consider that patients with HSCT are at high risk of acute encephalopathy due to chronic malnutrition, nausea induced by chemotherapy and vomiting 8 , by neurological alterations, including disorientation, mental state alteration, visual disturbances, and coma. 5However, it is yet unclear whether the use of these drugs during HSCT are risk factors for triggering such complications.
The consensus of the European Federation of Neurological Societies (EFNS) is that whole-blood thiamine (vitamin B1) measurement should be performed immediately prior to the administration of thiamine to confirm suspected or manifested WE, and MRI should be used to support the diagnosis. 9e conclude that WE should be considered in patients submitted to autologous HSCT associated with prolonged use of TPN and malnutrition.