Initial growth of dipteryx alata plants... INITIAL GROWTH OF Dipteryx alata PLANTS UNDER WATER DEFICIT

1 Received on 28.08.2015 accepted for publication on 31.10.2017. 2 Universidade Estadual de Goiás, Departamento de Produção Vegetal, Ipameri, Goiás Brasil. E-mail: <fabio.agronomia@hotmail.com>. 3 Universidade Estadual de Goiás, Programa de Pós-Graduação em Produção Vegetal, Ipameri, Goiás Brasil. E-mail: <igor_alberto99@hotmail.com> and <l.villasboass@gmail.com>. 4 Universidade Estadual de Goiás, Graduado em Agronomia, Ipameri, Goiás Brasil. E-mail: <daniel_guimaraes14@hotmail.com>. 5 Universidade Estadual de Goiás, Graduado em Engenharia Florestal, Ipameri, Goiás -Brasil. E-mail: <patyagrovida@yahoo.com.br>. *Corresponding author.


1.INTRODUCTION
The competitiveness in the Brazilian forestry sector, arising from ideal climate conditions and high technology application, places the country in an outstanding position in the world scenario (Ferreira et al., 2012).Brazil is the world's third greatest timber producer and the fourth producer of paper cellulose (CEPEA, 2014).Planted forests account for 1.2% of GDP, 24% of the agriculture and livestock sector GDP, employ 4.5 million people, and take only 1% of Brazil's productive land (Amazônia, 2015).The Brazilian forest vegetation cover is approximately 463 million hectares, 98.5% of which is estimated to consist of native forests located mostly in the country's North region.The remainder (1.5%) corresponds to planted forests.The predominant tree species in Brazilian planted forests are eucalyptus and pine (ABIMCI, 2014).Notwithstanding its high potential in the forestry sector, the country can further increase that production and transfer wealth to other economy segments through the expansion of the agricultural frontier.
Such expansion depends on the tolerance of species to the abiotic stresses prevailing in several regions of the country, such as the Northeast.Climate changes have extended water deficit periods in many parts of the world, and abiotic stress is the leading cause of low crop productivity worldwide, reducing by over 50% the average yields of most cultivated plants.In forests, water deficit is the most limiting factor to agriculture, as it reduces plant growth and increases their mortality (Zang et al., 2014).Working with forest species that tolerate drought may expand commercial exploitation of planted forests in areas considered inept due to low water availability.
As water resources become increasingly scarce, the development of drought-tolerating plants figures as a priority in obtaining high productivity.And drought tolerance is a result of various characteristics (anatomic, morphologic, physiologic, and molecular ones), which are expressed in different and concurrent ways depending on the water deficit severity and rate.The cultivation of species tolerant to water deficit will ensure income to producers from regions with semi-arid climate (Matos et al., 2014).This way, the exploitation of northeastern areas with planted forests may increase the Brazilian regional agriculture GDP.However, it is important to explore forest species other than traditional ones (eucalyptus and pine) in order to render the sector less vulnerable to weather and environmental conditions.Dipteryx alata stands out among promising forest species for field cultivation in different regions of Brazil.It is a country species easily adaptable to diverse types of soil and figures among the ten most promising native plants from the Brazilian cerrado for commercial cropping as planted forest, given its high germination rate, good seedling establishment, nitrogen fixation, timber yield, as well as medicinal, ornamental and feeding potential (Pacheco, 2008;Soares et al., 2008).The Dipteryx alata almond presents protein content superior to that of leguminous plants, being therefore recommended for feeding (Togashi et al., 1994).
According to Mosquetta et al. (2011), the Dipteryx alata almond is a low-cost bioadsorbent for the extraction of nickel (nickel enhances ethanol corrosive properties and increases soil, air and water pollution) with hydrated ethyl alcohol.The plant is original from Brazil, grows throughout the entire cerrado biome (Ratter et al., 2003), and yields high quality timber and fruits of commercial value (Cruz et al., 2011;Magalhães, 2014).The tree can grow up to 25m tall and reach 70-cm diameter at chest height when mature (Carvalho, 2010).
Multiple-application forest species like Dipteryx alata may represent an important commercial alternative, as they allow for complementary income from the harvesting and processing of their fruits (Leite et al., 2010).Brazil has a number of native forest species with economic potential, however the lack of scientific information is a major barrier to commercial cropping.Existing information regarding the development of Dipteryx alata under water deficit stress is insufficient to ensure economic pay-off in the field.The development of research to evaluate the species physiologic performance under drought conditions is required to provide basic understanding of the plant development in the field.Aiming to provide part of the lacking knowledge on the physiology of Dipteryx alata plants, this study was designed to evaluate the effect of water deficit stress on the early growth of Dipteryx alata plants.

2.MATERIAL AND METHODS
The research was carried out on a bench in full sun at the Goiás State University experimental unit in Ipameri Campus (17º43'19''S, 48º09'35''W, Alt.773m), Ipameri, Goiás.According to Köppen classification Initial growth of dipteryx alata plants... the region has tropical climate (Aw) with dry winter and rainy summer.Dipteryx alata seeds were collected from native plants in Ipameri municipality and sown in four-liter pots containing a mixture of soil, silt and cattle manure at ratio 3:1:0.5, respectively.Chemical analysis of the mixture showed the following values: pH 6.4; 19 g dm-3 of NOM; 2.4 mg dm-3 of P, 109 cmolc dm-3 of K, 1.5 cmolc dm-3 of H+Al, 3.2 cmolc dm-3 of Ca, 1.6 cmolc dm-3 of Mg, 27.7 mg dm-3 of Zn, 77.20% of BS, and 6.58 of CEC.After analysis of the mixture composition based on pH, availability of nutrients and organic matter, decision was made to not apply lime or carry out fertilization.
The experiment was set up according to the completely randomized design with five treatments (plants irrigated for 25 days with water volumes corresponding to 0%; 25%; 50%; 75%; 100% of daily evapotranspiration) and six replicates.The treatments were applied when the seedlings were 60 days old.Since the crop coefficient (kc) for Dipteryx alata has yet to be determined for the Ipameri region (GO), a kc of 1.00 was applied in accordance with the FAO-56 estimate (Allen et al., 1998) for a group of crops in the initial growth stage.The volume of water provided was estimated based on the reference evapotranspiration and crop coefficient.Crop evapotranspiration was determined using the equation:

ETc = ETo x kc
Where: ETc = crop evapotranspiration kc = crop coefficient ETo = Reference evapotranspiration Daily ETo was calculated by the FAO Penman-Monteith method (Smith et al., 1991) using daily data on minimum and maximum temperature, relative humidity, solar radiation and wind speed obtained from the INMET Weather Station located in the municipality of Ipameri, GO.
At 85 days after emergence the plants were assessed for: plant height, stem diameter, number of leaves, foliar chlorophyll concentrations (a+b) and total carotenoids, relative water content, transpiration, leaf, stem and root mass ratios, and total biomass.

2.1.Growth variables
The number of leaves, plant height and stem diameter were measured by counting, using a graduated ruler and a digital pachymeter.For destructive analyses, the leaves, roots and stems were extracted and ovendried at 72ºC to obtain constant dry mass.Then, they were weighed separately.The dry mass data allowed for the calculation of the leaf mass ratio (LMR), root mass ratio (RMR), stem mass ratio (SMR), and total biomass.

2.2.Relative water content and transpiration
The relative water content was determined by extracting ten 12-mm diameter foliar discs, which were weighed and saturated for four hours in Petri dishes with distilled water.Next, the discs were weighed again and dried at 70°C for 72 hours in order to obtain the dry weight in grams.Total daily plant transpiration was determined by the difference in weight of the pots.First, each pot was placed inside a plastic bag attached to the stem of the plant with a rubber band, leaving only the aerial parts (stem and leaves) exposed.Next, the pot (and bag) was weighed at 12pm (weight 1) and then again 24 hours later (weight 2).Total transpiration was estimated based on the difference between weights 1 and 2.

2.3.Photosynthetic pigments
In order to determine the total concentrations of chlorophylls and carotenoids (Cl a+b) two 1.0-cm foliar discs were extracted (third pair of fully expanded leaves) and placed in dishes containing dimethyl sulfoxide (DMSO).Subsequently, extraction was carried out in 65ºC water bath for three hours when the discs were completely discolored.Aliquots were extracted for spectrophotometric analysis at 490, 646 and 663 nm.Chlorophyll a and chlorophyll b contents were determined through the equation proposed by Wellburn (1994).

2.4.Statistic procedures
The experiment followed the completely randomized design, with five treatments and six replicates.The data were submitted to regression analysis using software R (R Core Team, 2015).

3.RESULTS
Analysis of the results showed little variation among treatments for foliar total carotenoids and root mass ratio.These variables did not present significant difference by F test, and the data did not conform to any significant regression model at 5% probability (results not shown).

MATOS FS et al.
Foliar concentration of chlorophylls (a+b), total transpiration and relative water content varied significantly among treatments (Figure 1).These variables presented values directly proportional to water availability and, consequently, they fit into the crescent linear regression model.
Growth variables -plant height, number of leaves, stem diameter and biomass -varied significantly among treatments (Figure 2).Vegetative growth was directly proportional to the water volume applied, and so variables showed crescent linear regression.
Stem and leaf mass ratios also varied significantly among treatments.The values for both variables increased according to the applied water volume (Figure 3).

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Initial growth of dipteryx alata plants... in regions with semi-arid climate.Resistance to abiotic stress is key to the survival and establishment of forest species in tropical ecosystems (Worbes et al., 2013).
It is important to note that regardless of the drought level rate, the plant Dipteryx alata survived.The significant reduction in transpiration and relative water content indicates a change in the hydration status of Dipteryx alata plants.The drastic reduction in the relative water content indicates that even under high stomatal control, the plant was unable to maintain high tissue hydration (as is the case with succulent stem species).

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to low water supply (Allen et al., 2010).The results corroborate those found by Hommel et al. (2014), who studied stomatal and mesophilic conductance in forest species and concluded that stomate closing in response to water deficit affects water use efficiency and decreases the photosynthesizing rate.Transpiration and biomass accumulation in Eucalyptus urogradis and other woody plants significantly decrease under water deficit (Pereira et al., 2006;Albuquerque et al., 2013).
T h e r e d u c t i o n i n f o l i a r c h l o r o p h y l l c o n c e n t r a t i o n m a y b e a s s o c i a t e d t o morphophysiological adjustment of the species in order to decrease the absorption of light energy, produce less photochemical energy and generate l es s re a c ti ve s p eci e s.It i s a ph ot op ro te c t io n mechanism of the species, since under water deficit stress the formation of free radicals that damage membranes and proteins is common (Matos et al., 2009).
In addition, it is noted that there is a strong relationship between the availability of nitrogen and activity of the reductase enzyme nitrate and this is extremely sensitive to water stress (Reis et al., 2007), thus less nitrogen may have been made available for the synthesis of pigments.Such alte rati o ns m ay be re l ate d to t he p he no ty p e plasticity of Dipteryx alata, since Brazilian cerrado species are naturally and constantly subjected to diverse combinations of climate and soil, and possibly some evolutionary need might have favored the species survival through morphophysiological adjustments.According to Palhares et al. (2010), the development of certain species under limiting conditions was due to evolutionary pressure that caused an important selection of plants in the Brazilian cerrado.
The results indicate that at the early development stage the vegetative growth of Dipteryx alata plants is highly decreased under water deficit stress.However, further studies with adult plants in the field are required to determine potential tolerance to drought, since recent research has found that drought tolerance may be associated to low tree growth rates (Rose et al., 2009;Taeger et al., 2013).