Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens

This survey aimed to evaluate the effects of dietary supplementation of different forms of savory essential oil (SEO) on growth performance, intestinal morphology and microbial population in broiler chickens. A total of 360 one-day-old male broilers were randomly allocated to 6 dietary treatment groups with 5 replicates per treatment and 12 birds per pen. The experiment consisted of a 2×3 factorial arrangement including two different forms [encapsulated SEO (ESEO) and nonencapsulated SEO (NSEO)] in three levels (0, 150, and 300 mg/kg diet) of SEO. Growth performance, jejunal morphology and intestine microbial population were examined. Our results revealed that feed intake was not influenced by the dietary treatments in different experimental periods. As well as, the experimental diets did not influence body weight gain (BWG) and feed conversion ratio (FCR) during the starter period. However, at the grower, finisher, and also whole rearing periods, broilers which received 150 mg/kg SEO had significantly higher BWG and lower FCR compared to the birds fed the control diet. The final body weight (FBW) was also higher in chickens fed with diet supplemented with 150 mg/kg SEO in comparison to the others. The results also revealed that 150 mg/ kg SEO, significantly increased the concentration of Lactobacillus and decreased the intensity of coliforms in the ileal digesta in comparison to the control diet. Furthermore, villus height was significantly lower in birds fed the control diet than in the birds that consumed different levels of SEO. Eventually, the findings of this experiment revealed that dietary supplementation of SEO, especially at 150 mg/kg level, was effective in raising the populations of beneficial microorganisms in the gastrointestinal tract as well as improving intestinal morphology and growth performance of broilers.


INTRODUCTION
From previous years, antibiotic growth promoters have been applied in the poultry industry to improve growth and health status in birds' diets. However, the expanding pressure on the poultry industry to clear feed-antibiotics as growth enhancers has commenced exquisite probing to find trusty and efficient alternatives to maintain intestinal health (Salajegheh et al., 2018). This novel generation of feed additives includes the herbs, spices and essential oils (EOs), which exhibits a wide range of attractive properties, such as antimicrobial, antioxidant, immune-modulatory and digestion stimulating properties (Brenes & Roura, 2010;Park & Kim, 2018;Oso et al., 2019).
Summer savory (Satureja hortensis L) is an aromatic and medicinal herb belonging to the Lamiaceae family. The essential oil of this herb (SEO) has distinct biological properties such as antimicrobial and antioxidant activities, which are associated with the existence of its major chemical compounds such as carvacrol and thymol (Vitanza et al., 2019). These properties aim to illuminate the improved performance observed in poultry. Anecdotally, EOs from various sources have been extensively expedited for their possible antimicrobial capabilities. Numerous studies in this field have stated that savory can also be included in the fowl nutrition as a feed additive to improve growth performance, blood indices, microflora population, intestinal morphology and even health status of birds (Ghalamkari et al., 2011;Zadehamiri et al., 2014;Rajaian et al., 2014;Yeganeparast et al., 2016;Dehghani et al., 2018).
The accomplished researches on the use of summer savory in poultry rations have produced conflicting results. One of the main reasons for this can be attributed to the fact that potency and bioavailability of these additives are mostly influenced by many variables such as intestinal pH, rate of absorption, dosage and chemical nature of the applied substance, animal species as well as the hygienic status of the production environment and buffering characteristics of dietary components (Wang et al., 2009;Yousaf et al., 2017). As well as, Lee et al., (2004) deduced that quality and efficiency of EOs might be reduced due to undesirable reactions, which can reduce palatability and feed consumption particularly when high levels of oil are available in the ration. Therefore, the use of these EOs primarily needs to find a proper method to reduce oxidative stability, control release, and improve the shelf life of these ingredients (Jafari et al., 2008).
Encapsulation is the operation of coating fragments or droplets of solid or liquid substances. In this procedure, a continuous film of polymeric material, like gums, chitosan, etc. is applied to produce capsules. Forasmuch as the effect of encapsulated SEO on broiler growth performance has not well been documented, we decided first and foremost to assess the chemical composition, in vitro antimicrobial and also antioxidant activities of SEO. Afterward, the efficacy of essential oil forms [encapsulated savory essential oil (ESEO) and non-encapsulated savory essential oil (NSEO)] on growth performance, intestinal morphology, and microbial population in broiler chicks was examined.

Composition of the SEO
The savory plant was collected from Kashan, Iran, and air-dried at ambient temperature. The dried materials were distilled using Clevenger distillation (Zaouali et al., 2010). The prepared essential oil was stored at 4° C in dark glass vessels. The preparation process of savory essential oils was done in a pharmaceutical company (Barij Essence Ardehal-Mashhad, Co., Kashan., Iran). The constitution of the oil was analyzed using gas chromatography (GC; Agilent 7890B, Agilent technologies) interfaced with mass spectrometry (MS; Agilent 5977A, Agilent Technologies, USA). The applied capillary column was the HP-5MS (5% phenyl methyl polysiloxane). The carrier gas was helium at a flow rate of 1 ml/min. The temperature of the column was adjusted at 5 ºC /min in the beginning, then programmed to increase up to 280 ºC at 5 ºC /min. the split ratio was 1:50. The mass spectrometer was run in electron-impact (EI) mode, using ionization energy of 70 eV. The essential oil was diluted 1:100 in n-hexane, after that, 0.1 µl was injected into the GC system. The components were recognized by comparing their relative retention times and mass spectra with the standard database NIST 80 (NIST, 2008) ADAMS (Adams, 2007) and Willey MS libraries (Adams & Sparkman, 2007). The GC peak areas were applied to the determination of the relative percentage of the oil constituents.

Encapsulation of SEO
The medium molecular weight chitosan (CS) and sodium triphosphate pentabasic (sTPP) were obtained from Sigma-Aldrich. Other reagents exerted in the trial were of analytical grade. Encapsulation of the savory essential oil was accomplished by ionic gelation based on the procedure of Sawtarie et al. (2017). Briefly, after dissolving CS in 1% (w/v) acetic acid, the solution sonicated before it became transparent. The dropwise increase of 10 ml triphosphate pentabasic solution (1 mg/ml) to a 25 ml CS solution (pH equal to 5), under constant shaking at usual temperature, produced the composition of CS-TPP parts by ionic gelation. Before adding the TPP solution, 20 % (w/v) SEO was subjoined to the CS solution due to the procurement of CS-TPP particles loaded with SEO.

Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens
containing different concentrations (0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, 12.8, 25.6, and 51.2 mg/ml) of the antimicrobial agent (ESEO and NSEO) was prepared and used 2fold dilution technique. One μl of 0.5 McFarland was placed on each of the series of plates. Then, inoculated plates were incubated at 35 ºC for 24 h, and the MIC values were obtained after 24 h. The MIC is the minimum concentration of NSEO that will obstruct the growth of microbes after nocturnal incubation (Wiegand et al., 2008).

In vitro antioxidant activity
The evaluation of the antioxidants activity of SEO based on the free radical-scavenging activity of the oils was performed based on DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical method. This activity was assessed according to the procedure of Burits & Bucar (2000). A volume of 1ml of the dilution of each sample in methanol was mixed with 2.5 ml of DPPH methanolic solution (0.004%, w/v). Then 200 µl of each sample (at various levels) were added to the microplate cells. A DPPH solution was used as a blank sample. After that, the microplate was kept for half-hour at lab temperature, in the dark. The absorbance of the samples was determined at 517 nm using a Power Wave TM HT Microplate Spectrophotometer (Bio Tek Instruments, Winooski, VT). This trial was carried out in 3 replicates. The antioxidant activity of the samples, which were described as percentage inhibition of the DPPH free radicals was estimated using the following equation: I % = (A blank-A sample / A blank) × 100 Where I symbol is the DPPH radical inhibition %; A blank and A sample are the absorbance values of the control (blank) and test sample, respectively. The IC50 value is distinguished as the level of the antioxidant needed for the 50% inhibition of the DPPH activity. The synthetic antioxidant reagent BHT was applied as a positive control.

Animals and experimental design
The animal care and use protocol was approved by the Research Ethics Committee of the Shahid Bahonar University of Kerman, Iran (IR.UK.VETMED. REC.1398.020). A total of 360 one-day-old (Ross 308) male chicks were purchased from a commercial hatchery and subjected to 6 dietary groups. Then, all birds were weighed and randomly housed into 30 cages (each cage of dimension 120 × 120 cm), consisted of 6 treatments, 5 replicates and 12 birds in each replicate. During the whole feeding period, birds were allowed ad libitum access to feed and water. The experiment was conducted in a completely randomized design, using a 2×3 factorial arrangement with two different forms of SEO (ESEO and NESO) at three levels (0, 150, and 300 mg/kg diet), which were added to a corn-soybean meal-based diet as a basal diet. Also, a 3-phase feeding scheme was utilized with a starter (1-10 d), grower (11-24 d), and finisher (25-42 d) phases. The gross composition of the basal diets is presented in Table 1. Diets were adjusted according to the standards described in Ross 308 broiler nutrition specification (Aviagen, 2014).

Growth performance
The growth performance parameters were obtained in the starter, grower, finisher, and overall (1 to 42 d) periods. On days 1, 10, 24, and 42 of age, the birds were weighed, and the weight of feed residuals per pen was registered. These data were applied to measure BWG and FI. FCR was also determined by BWG and FI. The mortality rate was registered daily.

Intestinal microflora population
On day 42, two male chicks per replicate were randomly opted and slaughtered for intestinal microflora analyzes. The ileal contents of the birds eRBCA-2020-1284

Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens
were attentively depleted into a sterilized bottle and immediately transferred to the laboratory. After that, serial dilutions were performed during one hour of the collection (10¯¹ dilution as the primary dilution up to 10¯9 as the ultimate dilution). Optional agar media was employed for the enumeration of bacterial target populations: Lactobacillus bacteria (MRS agar-Merck, Darmstadt, Germany); and total coliform bacteria (Mac Conkey Agar-Merck, Darmstadt, Germany) (Yang et al., 2012). Incubated samples under anaerobic and aerobic conditions (37 ºC for 72 h), were applied for the determination of the total numbers of Lactobacillus and coliform respectively (Adaszynska-Skwirzynska & Szczerbinska, 2018). Obtained findings were expressed as log10 CFU/g ileal digesta.

Intestinal morphology
For intestinal morphology evaluation, two male birds per pen were killed by cervical dislocation at the end of the trial. The 2-cm segments of the jejunum were obtained and fixed in 10 % buffered formalin for 24 h, and then the 10 % buffered formalin was renewed. After dehydration, samples were placed into xylol and embedded in paraffin. A microtome was applied to make five cuts that were stained with hematoxylin-eosin. Villus height was determined from the apex of the villus to the valley in the middle of individual villi, and the crypt depth was measured from the valley between individual villi to the basolateral membrane. The values of the villus height (VH), crypt depth (CD), and villus width (VW) were determined 5 times from various villus and crypts per slide (Thompson & Applegate, 2006).

Statistical Analysis
The experimental design was a completely randomized design, using a 2×3 factorial arrangement with two forms of SEO and three levels of SEO. The data were analyzed by ANOVA using the GLM procedures of SAS statistical software (SAS, 2001). Treatments were compared applying the Tukey's test, and the differences were judged statistically significant at p≤0.05.

SEO Chemical characterization
The chemical composition of SEO was determined by a GC-MS instrument, in which the retention times and the mass spectra of oil ingredients accompanied by the data library, as exhibited in Table 2. According to the data, eighteen compounds identified by GC-MS, so that carvacrol (50.46%), γ-terpinene (16.91%), thymol (11.74%), and p-cymene (8.14%) were obtained as the major constituents of the SEO.

Minimum inhibitory concentration (MIC)
The results of the MIC method (as shown in figure 1) indicated that SEO has antibacterial activity against pathogenic bacteria, including E. coli and S. aureus. Both of them were more sensitive to the ESEO in comparison to the NSEO. The MIC value of ESEO against E. coli and S. aureus were 3.2 and 0.2 mg/ml, and for NSEO were 0.8 and 0.2 mg/ml, respectively. Among pathogenic bacteria, E. coli was the most sensitive to NSEO and ESEO, while S. aureus was the most resistant.

Antioxidant activity analysis
Antioxidants characteristics of SEO based on free radical-scavenging activity are depicted in figure 2. This activity was assessed according to the method of Burits & Bucar (2000). The values of IC50 for NSEO and ESEO and BHT were 68.895, 384.085, and 20.30 µg/ ml, respectively. The findings show that the antioxidant activity of ESEO was significantly higher than the NSEO.

Growth performance
The birds were healthy throughout the experiment, with mortality of <2% that was uncorrelated to the dietary treatments. The effects of the experimental treatments on broilers performance are demonstrated in Table 3. Feed consumption was not influenced by the dietary treatments in any of the various phases of the rearing (p>0.05). As well as, BWG and FCR were not affected by the experimental diets during the starter period (p>0.05). However, at the grower (p<0.05), finisher (p<0.01), and also the whole rearing period (p<0.01), the broilers which received 150 mg/ kg SEO had significantly higher BWG and lower FCR compared to the birds fed the control diet. Similarly, the birds which received 150 mg/kg SEO had a higher FBW in comparison to other birds (p<0.01). As reverse, throughout the different phases of the experiment, there were no differences between chicks fed 300 mg/kg SEO with those fed the control diet (p>0.05). Furthermore, the interaction between the SEO level and SEO form was not significant (p>0.05) except for BWG in the overall growing period and FBW (p<0.05). Regarding these interaction effects, the highest values were observed in birds fed 150 mg/kg NSEO and the lowest values related to those fed the control diet.

Ileal microbial population
The effects of the experimental diets on the ileal microbial population are illustrated in Table 4. Dietary inclusion of 150 mg/kg SEO, significantly increased

Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens
the concentration of Lactobacillus and decreased (p=0.002) the concentration of coliforms (p=0.010) in the ileal digesta in comparison to the control birds. SEO form and its interaction with the SEO level did not affect the microbial population of the ileum (p<0.05). SEM standard error of the means. SEO Level = Savory Essential Oil (0, 150 and 300 mg/kg). NE = Non-Encapsulated, E = Encapsulated.

Intestinal morphology
The effect of different treatments on the intestinal morphology indices at 42 d of age is summarized in Table 5. Unlike villus width, crypt depth, and villus height influenced by the dietary SEO level. The villus height was significantly lower in chicks fed the control diet than in the birds that consumed different levels of SEO (p<0.01). The crypt depth was also significantly higher in broilers fed 150 mg/kg SEO in comparison to those fed the control diet (p<0.05). SEO form, as well as the interaction between SEO level and SEO form, did not affect the morphological characteristics of the intestine (p<0.05).
It turns out that the concentrations of SEO components such as linalool, carvacrol, g-terpinene, p-cymene, and b-caryophyllene exhibit broad variations depending on environmental conditions, geographic origin and the stage of herb development (Milos et al., 2001).

Antibacterial activity
The results of this survey demonstrated that SEO has more antimicrobial activity on gram-negative bacteria

Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens
(E. coli) in comparison to the gram-positive bacteria (S. aureus). These activities have been ascribed to the presence of active volatile components in the SEO. Nowadays, it has become clear that thymol and carvacrol play a vital role in antimicrobial activities against tested strains. Many findings have shown that the important constituents of the Satureja spp, contain thymol and carvacrol, which are mostly accompanying γ-Terpinene, paracymene, and linalool. This class of phenolic compounds has antimicrobial activates (Mirjana & Nada 2004;Sefidkon & Jamzad, 2005). Although, it's usually expected that EOs should be more efficient against gram-positive bacteria because of the fundamental interactions between the cell membrane and hydrophobic ingredients of the EOs (Soković et al., 2010). However, in an experiment by Dorman & Deans (2000), phenolic compounds include thymol, and carvacrol reacted otherwise against grampositive and gram-negative bacteria, which was in line with the results of the present study. In this regard, Burt (2004) declared that thymol and carvacrol can lead to the collapse of the external membrane of gram-negative bacteria, releasing lipopolysaccharides and amplifying the permeability of the cytoplasmic membrane to ATP. Concerning the mechanism of action, it's believed that phenolic compounds interfere with the cell membrane role and eventually result in the leak of ions, and thus, they operate their bactericidal function. The chemical structure of herbal essential oil and its volatile compounds have a great impact on the antimicrobial activities of EOs. Bacterial count, type of culture medium, extraction manners, growth phase, pH, incubation time and also temperature are the main factors affecting the antimicrobial activities of the herbal EOs, so the findings obtained from different experiments are sometimes accompanying with differences (Burt, 2004).
On the other hand, the broilers receiving ESEO showed higher microbial activity against the E. coli population compared to birds that received NSEO. These data revealed that the supplementation of ESEO prevented more the growth of the pathogen in the broiler intestine. In line with our findings, Michiels et al., (2008) exhibited that carvacrol was wholly imbibed in the abdomen and the proximal small intestine of piglets after oral consumption, obviously stipulating the need for insulating carvacrol and efficient transport to the poultry intestine. Although, it seems synergism between EOs and different processing methods will also require to be more probed before they can be exerted commercially (Calo et al., 2015).

Antioxidant activity
The main constituents of the Satureja species such as carvacrol and thymol are extensively reported to possess high levels of antioxidants (Ćavar et al., 2008;Oke et al., 2009;Khoury et al., 2016). Moreover, the synergistic effects functions between the various molecules of the EOs and their monoterpenoid components have been published (Bakkali et al., 2008). Trifan et al., (2015) indicated that phenolic chemotype (carvacrol) existing in SEO was exhibited a considerable antioxidant activity with potential applicability in the preservation of sensitive matrices from free radicalmediated oxidative stress, comprising ionizing radiation-induced oxidative damage. Fazel et al., (2007) also declared the antioxidant activity of the essential oils of savory based on IC50 was 5.8 mg/ml, whereas IC50, 68 µg/ml in our survey showed good antioxidant activity. Our findings also demonstrated that ESEO had more antioxidant properties than the NSEO. It seems the antioxidant properties of essential oils linked to their capability to act as an anti-inflammatory factor. A large amount of reactive oxygen species (ROS) are produced by monocytes, neutrophils, eosinophils, and macrophages through the process of bacterial phagocytosis. ROS oxidative damage on biological macromolecules such as proteins, lipids, and DNA is considered as the initial phase of various diseases, aging, and cancer (Dickinson et al., 2011). It is proven that essential oils can scavenge ROS and thus decrease the oxidative damage of a tissue that has been linked to the reduction of inflammation (Miguel et al., 2010). The difference between these antioxidant capacities is probably due to the insolubility of ESEO in methanol.

Growth performance
As noted in Tables 3, except for the starter phase, in other periods, broilers which received 150 mg/kg SEO had significantly higher BWG and lower FCR compared to the birds fed the control diet. The FBW was also higher in chicks which received 150 mg/kg SEO in comparison to the others. Whereas, all of these parameters for chicks treated by 300 mg/kg SEO were similar to those fed the control diet.
Although sufficient information on the encapsulated SEO and its possible use as an alternative to antibiotics for poultry is not available, few studies on savory utilize in poultry nutrition have been carried out in recent years. The obtained findings of this experiment in regard to using 150 mg/kg SEO, were in agreement with the results of Zamani Moghaddam et al., (2007), and Goodarzi et al., (2014)

Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens
improvements in FCR, BWG and FBW of broilers, due to the utilization of SEO. Rajaian et al., (2013) also stated that Satureja hortensis powder has a positive effect on FBW and no significant impact of FI and FCR. In another experiment whose results were the opposite of Rajaian et al., (2013), it was concluded that, unlike BWG that was not impressed by savory extract, FCR was significantly improved when birds fed 400 mg/kg savory extract, compared to control birds (Movahhedkhah et al., 2019). This part of our results also disagreed with the experiments carried out by Ghalamkari et al., (2011), andYeganeparast et al., (2016), who claimed that SEO did not have a positive effect on the growth performance of broilers. However, these reports are consistent with our data regarding the use of 300 mg/kg SEO, which did not make any significant difference with the control group. Similarly, Zamani Moghaddam et al., (2007) concluded that although the use of 3 g/kg savory had an advantageous effect on growth performance in broilers, higher dosage had a detrimental impact on performance. In another experiment similar to ours, Azarbad et al., (2019) reported that adding various levels of Satureja essential oil (400 and 500 mg/ kg) in conventional and microencapsulated forms to the diet of broilers did not affect feed consumption and FCR of broilers. These authors also showed that experimental treatments containing conventional and microencapsulated types of Satureja essential oil caused a significant decrease in the final body weight of the birds relative to the control treatment. These researchers attributed these findings to the presence of polyphenolic compounds, such as carvacrol, which is known as a bitter-tasting substance. It has also been claimed this active compound can modulate appetite and reduce FI by affecting the central nervous system (Brenes & Roura, 2010;Azarbad et al., 2019).
These differences in the mentioned results could be attributed to the combined effect of variations in the sample type and dose, duration and processing of the herb, EOs, different dietary compositions, environment, management, and age differences (Zeng et al., 2015). Despite all these contradictions, it is generally believed that EOs have numerous lucrative functions in poultry diet, involving increased FI (Jamroz et al., 2005;Jang et al., 2007), digestibility improvement (Lee et al., 2003), secretion of digestive enzymes (Jamroz et al., 2005;Lee et al., 2003), and modification of gastrointestinal microflora (Liolios et al., 2009). Also, other reasons, such as growth-stimulating of broilers via enhancement of glucose influx into the tissues (Goodarzi et al., 2013), and elevation of serum testosterone concentration (Khosravinia, 2014), have been mentioned in the interpretation of improved bird performance. These factors appear to be able to conspicuously vindicate the performance improvement of the chicks which received 150 mg/kg SEO. The utilization of 300 mg/ kg SEO appear to have had no significant effect on performance indices, according to Zamani Moghaddam et al., (2007). As mentioned earlier, carvacrol found in SEO can modulate appetite and decrease the growth performance of birds by affecting the central nervous system. Also, with the increase in SEO content in the diet, the relative decline in broilers performance due to the bitter taste of carvacrol will not be surprising (Brenes & Roura, 2010;Azarbad et al., 2019).

Ileal microbial population
Consistent with the in vitro findings, our results, confirmed that all the bacteria were susceptible with different degrees to SEO. Our data were at variance with the outcomes detailed by Yeganeparast et al., (2016), revealing the ineffectuality of 150 mg/kg SEO on the intestinal microflora population. Although there are not many reports on the effect of this essential oil on the intestinal microflora, other ESOs have registered impacts in this area. Many researchers believed that phytogenic additives cause positive changes in gut microbiota by restricting the growth of harmful bacteria, reduction of their metabolites, growth improvement of the beneficial bacteria, enhancement of the feed breaks down, and increasing nutrient availability to the host animal (Jamroz et al., 2003;McReynolds et al., 2009;Tiihonen et al., 2010;Mountzouris et al., 2011;Oso et al., 2019). However, other studies have not exhibited significant effects of herb-byproducts on the gut microbiota of birds (Cross et al., 2007;Cao et al., 2010;Kirkpinar et al., 2011).
SEO and its active constituents (especially carvacrol and thymol) have been shown to exhibit a wide spectrum of activity against microorganisms (Mirjana & Nada, 2004). The antimicrobial mode of action is mainly attributed to ESO's potential to entrance into the bacterial cell membrane, collapse these constructions, and cause intracellular materials leakage. These additives were also claimed to stimulate intestinal secretion of mucus in broilers, which was supposed to reduce the adhesion of pathogens (Jamroz et al., 2006). These interpretations support the theory that phytogenic additives may positively modify gut functions, howbeit the number of in-vivo studies with birds is still quite finite.

Efficacy of Savory Essential Oil Utilization in Conventional and Encapsulated Forms on Performance of Broiler Chickens
On the other hand, unlike the in vitro data, in vivo results demonstrated that SEO form had no in vivo effects on the viability of Lactobacillus spp. and E. coli in the ileum of the bird.

Intestinal morphology
For years, there has been a consensus that, mucosa status and their microscopic anatomy can be proper indices of the intestinal tract reaction to active substances in diets. As depicted in Table 5, villus height was significantly higher in birds consumed different levels of SEO in comparison to the birds fed the control diet. The crypt depth was also significantly higher in broilers fed 150 mg/kg SEO in comparison to those fed the control diet.
In a similar experiment, Azarbad et al., (2019) illuminated that adding different levels of Satureja essential oil (400 and 500 mg/kg) in conventional and capsulated forms (0.5 and 1%) to the diet of broiler chickens had no effect on the width of villi, depth of crypt and length of villi to depth of crypt ratio was reported. These authors also remarked the level of 0.5 percent capsulated Satureja essential oil caused a significant decrease in length to width of the villi ratio. Although the reason for that is not yet fully understood, these authors attributed the lack of efficacy to factors such as inadequate or incorrect use of active plant ingredients, and specific conditions and different responses in the animals tested.
Insomuch, increased villus height is associated with improved gut health, the diets containing 150 and 300 mg/kg SEO, offer a relative privilege over the control diet in modifying the gut health status of the broilers in the present study. As was the case with performance, almost the same trend was observed, and there was a reasoning match in this regard. It's supposed that an elevated villus height is collimated by an enhanced absorptive and digestive activity of the intestine as a consequence of increased absorptive surface area, activation of brush border enzymes, nutrient transfer systems and a subsequently improved performance (Caspary 1992;Iji et al., 2001;Montagne et al., 2003;Maneewan & Yamauchi, 2004;Oso et al., 2019).
Although encapsulation has been proposed as an impressive approach for use in targeted delivery to the gastrointestinal tract, our data failed to evidence any positive effect of this process on growth performance, intestinal morphology, and microbial population in broilers. If we want to summarize, this ineffectiveness related to factors such as inadequacy of the active plant material, incorrect utilizes of the samples, inappropriate concentration of the applied materials, specific conditions, and different responses of the treated animals and other things like that. More research is needed to prove the efficacy of this essential oil and its encapsulated form in poultry nutrition.

CONCLUSIONS
It can be concluded that dietary supplementation of SEO, especially at 150 mg/kg level, was effective in raising the populations of beneficial bacteria in the gastrointestinal tract as well as improving intestinal morphology and growth performance of broilers. These facts may have a prominent influence on the physiology and biochemistry of the intestine. However, our data failed to demonstrate any positive effect of ESEO in comparison to NSEO form on growth performance, intestinal morphology, and microbial population in broilers. Subsequent animal surveys will supply better insight into the potential of these bioactive substances to operate as prebiotics in the host's intestinal tract.