Physical , chemical , microbiological and sensory carachterization of Manipuça jellies for municipal school meals of Fortaleza-CE Lidiane

This research aimed to characterize the physical, chemical, microbiological and sensory characterization of manipuçá (Mouriri cearensis Huber) jellies as an alternative to school meals of an elementary public school in Fortaleza-CE. The fruits, in maturation stages 4 (mature) and 5 (senescent), were collected in the Botanical Garden of São Gonçalo, in São Gonçalo do Amarante-CE. Initially, the fruits of manipuça were processed and to obtain the jelly five formulations were proposed (treatments) containing different proportions of water and pulp (4:1-F1; 4:2-F2, 4:3-F3: 4:4-F4; and 4:5-F5), which then they were subjected to physicochemical analysis (total soluble solids-TSS; total titratable acidity-TTA; pH; TSS/TTA ratio-SAR; vitamin C-CV; total soluble sugar-TSSu, reducing sugar-RS and non-reducing sugars-NRS), microbiological (coliforms at 35°C-CF) and sensory (aroma-A; color-C; appearance-AP; flavor-F; texture-T; and the purchaser intends-PI). We used the Assistat program in statistical analyzes. The results showed the following variation in the five formulations: TSS (°Brix) 72 (F1) to 85.12 (F5); TTA (%) 0.95 (F1) to 0.54 (F5); pH 4.09 (F1) to 4.30 (F5); SAR 75.22 (F1) to 156.20 (F5); CV (mg / 100g) 7.40 (F2) to 14.19 (F5); TS (%) 63.97 (F1) to 79.73 (F5); RS (%) 44.53 (F2) to 66.98 (F5); NRS (%) 12.47 (F1) to 32.98 (F4); absence of CF; for A, C, AP, F and T, F5 was greater than F2, F3, F4 and equals to F1; and for PI, F5 was superior to all. In conclusion: the F5 is sweeter and less acidic, however, the F1 is less sweet and more acidic; the F5 obtained greater acceptance among students, so this is the ideal formulation to use as an alternative to school meals for primary school students from public schools in Fortaleza-CE.

L. M. F. Azevedo et al.

Introduction
The fruit farming is present in all Brazilian states and as an economic activity employs around 5.6 million people, directly and indirectly.As a result, Brazil ranks as the third largest producer in the world, harvesting around 43 million tons of fresh fruit per year, 53% of which is destined for the fresh fruit market and 47% for processed fruit, but only 3% of the national harvest is exported in natura and 26% is exported processed, which shows the strong internal consumption (ANUÁRIO BRASILEIRO DE FRUTICULTURA, 2016).
On the coast of Ceará, the native fruits occupy a prominent place in the diverse ecosystems and, in general, their fruits are commercialized in the regional market with great popular acceptance, playing an important role in the population and native animal nutrition.One of these fruits is the manipuçá (Mouriri cearensis Huber).Also known as puçá, it belongs to the Melastomataceae family, which vegetates in Restinga environment and is native to Ceará.The fruits of the manipuçá are very appreciated, when consumed in natura, by the communities of the coast of Ceará (LUCENA et al., 2011b).
In the dynamic market economy, the development of new products is an essential factor for the survival of companies, especially those of food, which often need to release new products in order to remain ahead of an increasingly competitive market.Consumers today have raised their expectations for new products and have reduced their loyalty to traditional brands, which makes the food market even more competitive (DAMIANI et al., 2012).In this sense, the manipuçá is a good choice of raw material for the production of jelly, since the jellies constitute an important alternative for the use, processing and consumption of fruits (BASU et al., 2013).
The jelly of the manipuçá can be a viable alternative to complement the school meal of students of public schools, because according to Bezerra and Arimatea (2009), the soup, although it is a meal that the students can like, is a food served repeatedly, which the students dislike.This is due to the fact that the monetary value transferred to schools is too low for a diversified diet.
Given the above, this research aimed to make the physical, chemical, microbiological and sensorial characterization of manipuçá jelly as an alternative to school meal of elementary school of Fortaleza-CE municipal public schools.

Materials and methods
The study was conducted at the Vegetable Ecophysiology Laboratory-ECOFISIO, of the State University of Ceará-UECE, at the Laboratory of Post-Harvest Physiology and Technology of Embrapa Tropical Agroindustry and at the Experimental Nucleus in Science and Technology of Regional Food of the UECE, all in Fortaleza-CE, from February 2015 to July 2016.
The fruits, characterized according to the peel color in stage 4 (ripe -90% orange and 10% light green) and 5 (senescent -100% orange) of maturation (GONÇALVES et al., 2017), were harvested manually and randomly from 10 matrices previously chosen in the São Gonçalo Botanical Garden, in São Gonçalo do Amarante-CE, located between the coordinates 3º 36' 26" south latitude and 38º 58' 06" west longitude, consisting of Restinga.Adult, healthy and uniform maturing plants were selected.A flow chart of the jelly processing can be seen in Figure 1.Physical, chemical, microbiological and sensory carachterization of Manipuça jellies...The fruits were transported to ECOFISIO, where they were received, manually selected, weighed and washed in chlorinated water at 20 ppm.The pulp extraction was carried out in an industrial blender for five minutes, using the entire fruit, that is, with peel, pulp and seed.The amount of water used for extraction was twice the mass of the fruits.50% of the fruits were harvested at stage 4 and 50% at stage 5.The sieving was then done in stainless steel sieves; the pulp was packed in low-density polyethylene bags and frozen in a freezer at -18°C for later use.
The jellies were packed in polypropylene jars, sealed and identified.The physical, chemical, microbiological and sensory analyzes of the jellies were then carried out.
The physical and chemical analyses realized were: total soluble solids (TSS), according to the methodology recommended by AOAC (2005); total titratable acidity (TTA), by titration, according to Brasil (2005a); pH, according to AOAC (2005); TSS/TTA relation (SAR), obtained by the quotient between the two variables; C Vitamin (CV), obtained by titrimetry, according to the methodology of Strohecker and Henning (1967); total soluble sugars (TSSu) by the antrona method, according to the methodology described by Yemn and Willis (1954); reducing sugars (RS), determined by the DNS method (dinitrosalicyclic), according to the methodology described by Miller (1959); non-reducing sugars (NRS), which were obtained by the difference between the TSSu and the RS, according to Lucena et al. (2011a).The presumptive test for coliforms was also necessary to carry out, that is coliforms at 35ºC (SILVA et al., 2007), in order to guarantee the safety of the tasters in the sensorial analysis.
The sensory evaluation of the jellies was carried out with 50 untrained elementary students from the José Valdevino de Carvalho Municipal School, located at Rua Guará, S/N, Itaoca Neighborhood, Fortaleza-CE, Municipal Education Department, District IV.The tasters assessed aroma, color, appearance, flavor and texture using the nine-point structured hedonic scale, the extremes being "extremely enjoyed" (9) and "extremely disagreeable" (1).For the purchase intention, we used the structured scale of five points, whose extremes were: "I would certainly buy it" (5) and "I certainly would not buy it" (1) (IAL, 2008).
The experimental design was completely randomized, with five treatments (formulations) and four or fifty repetitions, depending on the analysis (physical-chemical or sensorial, respectively).The results were submitted to the analysis of variance observing the significance by the F test and when significant, the Tukey test was carried out at the 5% probability level, using the Assistat 7.7 beta statistic program.

Results and discussion
The total soluble solids (TSS) in the manipuçá jelly increased with the increase of the formulation concentration (F), increasing from 72°Brix in formulation 1 (F 1 ) to 85.12°Brix in formulation 5 (F 5 ) (Table 2).Although the added sugar mass was calculated in order for all formulations to have proportionally the same amount of sugar (40%), the concentration time increased with the increase of the formulation concentration, since it took more time to evaporate the water and, consequently, promoted a higher concentration of TSS.

L. M. F. Azevedo et al.
The titratable total acidity (TTA) in the manipuçá jelly was decreasing with the increase of the formulation concentration, decreasing from 0.95% of citric acid in F 1 to 0.54% of citric acid in F 5 (Table 2).This behavior occurred because the amount of pulp added in the formulations was increasing, while the citric acid was constant, therefore, the higher dilution caused the reduction of the acidity.
The highest TTA value in the jelly of this research was higher than those found in murici jelly, with 0.50% of citric acid (MONTEIRO; PIRES, 2016), in mixed pineapple peel with peach pulp jelly, with 0.25% of citric acid (VIEIRA et al., 2017), in the umbu-cajá Suprema genotype jelly with 0.73% of citric acid and in the Preciosa genotype, with 0.66% of citric acid (VIANA et al., 2015).On the other hand, it is inferior to that found in extra graviola jelly with pepper, with 1.65% of citric acid (TELES et al., 2017) and cagaita jelly (Eugenia dysenterica DC.), with 1.28% of citric acid (SANTOS et al., 2012).
The pH in the manipuçá jelly increased with the increase in the formulation concentration, increasing from 4.09 in F 1 to 4.30 in F 5 (Table 2).This is probably due to the amount of pulp added in the formulations have been increasing while the citric acid was constant, therefore, the higher dilution caused the acidity reduction and consequently the pH rise.
The pH values obtained in the manipuçá jelly of this research were higher than those found in conventional jelly of umbu-cajá Suprema genotype, with pH of 2.36 and in the Preciosa genotype, with pH of 2.42 (VIANA et al., 2015), in murici jelly stored at room temperature, with pH of 3.43 (MONTEIRO; PIRES, 2016), in extra graviola jelly with pepper, with pH of 3.69 (TELES et al., 2017) and mixed pineapple and peach pulp jelly, with pH 3.50 (VIEIRA et al., 2017).
The TSS/TTA ratio (SAR) in the manipuçá jelly was increasing with the increase in formulation concentration, increasing from 75.22 in F 1 to 156.20 in F 5 (Table 2).The TSS/TTA ratio is more representative than the isolated measurement of sugars or acidity, since this ratio, besides giving a good idea of the balance between these two components, indicates the degree of sweetness.
The highest value above the SAR in the manipuçá jelly was higher than that found in murici jelly stored at room temperature with SAR of 129.60 (MONTEIRO; PIRES, 2016), in a conventional umbu-cajá jelly Preciosa genotype with SAR of 98.48 in the Suprema genotype, with SAR of 89.17 (VIANA et al., 2015) and in extra graviola jelly with pepper, with SAR of 39.39 (TELES et al., 2017).On the other hand, it is inferior to that found in mixed pineapple peel with peach pulp jelly, with SAR of 260.00 (VIEIRA et al., 2017).
The C Vitamin (CV) in the manipuçá jelly was increasing with the increasing formulation concentration, increasing from 10.72 mg of ascorbic acid/100 g of jelly in F 1 to 14.19 mg ascorbic acid/100 g of jelly in F 5 (Table 2).Although there is no recommended value for CV for jellies in Brazilian legislation, the values obtained in this research partially meet the need for daily recommended intake of CV for adults, which is 45 mg according to RDC n. 269 (BRASIL, 2005b) because they contain this amount in more than 100 g of jelly.Therefore, if we consider the lowest and the highest value of CV found in this study, 608.10 g of F 2 and 317.12 g of F 5 of manipuçá jelly must be consumed to meet this need.
The results of CV in the manipuçá jelly observed in this study were significantly lower than that found by Assis et al. (2007), in cashew jelly (Anacardium occidentale L.) at time zero, which was 184.01 mg of ascorbic acid/100 g of the sample and in cagaita jelly, in which Santos et al. (2012) obtained 31.22 mg of ascorbic acid/100 g of the sample.On the other hand, they are superior to that found in extra graviola jelly with pepper, which was 0.889 mg of ascorbic acid/100 g of the sample (TELES et al., 2017).
The total soluble sugars (TSSu) in the manipuçá jelly were increasing with the increase in formulation concentration, increasing from 63.97% in F 1 to 79.73% in F 5 (Table 2).According to the increase of TSSu in the formulations, we found that although the proportional amount of sugar added was the same, the added pulp volume was increasing, thus, it took longer to evaporate the water and consequently provided a concentration of TSSu.
The lowest value of TSSu (63.97%) in the manipuçá jelly presented in this study was similar to the results found by Souza et al. (2015) in jellies obtained from blackberry fruits harvested at 3 different harvest stages, which were 60.08% with 100% red fruits, 61.84% with 50% red fruits and 50% black, 59. 54% with 100% black fruits.On the other hand, it was superior to that obtained in mixed pineapple peel with peach pulp jelly, with 44.56% (VIEIRA et al., 2017), in conventional umbu-cajá jelly Preciosa genotype, with 56.28 and in the Suprema genotype, with 57.05% (VIANA et al., 2015).
The reducing sugars (RS) in the manipuçá jelly decreased from F 1 (51.49%) to F 2 (44.53%) and then increased up to F 5 (66.98%) (Table 2).The increase of RS in the formulations was expected because the longer the concentration time is, the greater the inversion will be, the hydrolysis of sucrose into glucose and fructose.
The RS values in the manipuçá jelly verified in this research are superior to those found in jellies obtained from blackberry fruits harvested in 3 different stages of harvest, that were 36.09% with 100% red fruits, 37.74% with 50% red fruits and 50% black, 34.94% with 100% black fruits (SOUZA et al., 2015) and mixed pineapple peel with peach pulp jelly, with 18.90% (VIEIRA et al., 2017).
Rev. Bras. Frutic., Jaboticabal, 2018, v. 40, n. 1: (e-728) Physical, chemical, microbiological and sensory carachterization of Manipuça jellies...The NRS results of F 1 and F 5 in the manipuçá jelly of this research were lower than that found in jellies obtained from blackberry fruits harvested at 3 different harvest stages, which were 23.99% with 100% red fruits, 24.10% with 50% red and 50% black fruits, 24.60% with 100% black fruits (SOUZA et al., 2015) and mixed pineapple peel with peach pulp jelly, with 25.66% (VIEIRA et al., 2017).Only the F 4 was superior to the results obtained by the aforementioned authors.
For all samples of the presumptive test for coliforms, the results were negative for coliforms at 35ºC.Although the RDC n. 12 (BRASIL, 2001) does not mention targets to be reached in the research of coliforms for jelly, they may indicate deficiencies in the heat treatment (HAJDENWURCEL, 1998).The results suggest that there were good procedures in the processing of jellies, such as adequate sanitation of fruits and equipment used, as well as the effectiveness of the conservation methods used.
In the sensory acceptance test (Figure 2) for aroma, color, appearance, taste and texture, the F 5 was significantly higher than F 2 , F 3 and F 4 , while F 5 did not differ significantly from F 1 .Only for the intention of purchase test, there was a significant superiority of F 5 in relation to the other formulations (F 1 , F 2 , F 3 and F 4 ).
According to Assis et al. (2007), studying three formulations of cashew jellies with clarified juice concentrations of 20, 15 and 10ºBrix, we found that there is a difference in flavor among the formulations tested, with 10º Brix being the most preferred followed by 15º Brix and the less preferred with 20ºBrix.These results are contrary to those of this research with manipuçá jelly, in which the formulation that presented the best sensory results was the one with the highest TSS content (F 5 -85, 12ºBrix).
On the other hand, Viana et al. (2015) evaluated the intention to buy of four formulations of conventional and dietary umbu-cajá jellies, they verified that 70% of the consumers indicated that they would buy the conventional jelly formulation elaborated with the Suprema genotype, that is, the formulation of jelly with higher TSS (61.10%) and TSSu (57.05%) was preferred among tasters, such as the F5 in this study with jelly, with levels of TSS (85.12%),TSSu (79.73%) and RS (66.98%) higher than the other formulations (F 1 , F 2 , F 3 and F 4 ).Santos et al. (2012), investigating four formulations of cagaita jelly through the use of two types of pulp (filtered or unfiltered) and two proportions of pulp:sucrose:pectin (50:50:0.2and 60:40:0.1),they found that there was no significant difference between the jelly formulations for flavor, color, aroma, texture and overall impression attributes (p>0.05),although in this study with manipuçá jellies, significant differences were obtained for the aroma, color, appearance, taste, texture and purchase intention attributes.Vieira et al. (2017), studying the acceptability of mixed pineapple peel with peach pulp jelly, found that all the attributes evaluated in the acceptability test obtained average scores between 8 (I liked it very much) and 9 (I liked it a lot), indicating high acceptance of the product, which was corroborated by the alleged intention to buy, which was 91%.These results were similar to those of this study for the F 5 of manipuçá jelly, because for aroma, color, flavor and texture, the acceptability was also between 8 (I liked it very much) and 9 (I liked it a lot), which was corroborated by the intention to buy of 4.02 (I would probably buy it).
L. M. F. Azevedo et al.

Conclusions
The total soluble solids (TSS), the pH, TSS/ TTA ratio (SAR), C vitamin (CV), total soluble sugars (TSSu) increased with the increase in the concentration of manipuçá jelly formulation (F), however, the titratable total acidity (TTA) was decreasing.On the other hand, reducing sugars (RS) and non-reducing sugars (NRS) showed fluctuations with the increase in the concentration of the manipuçá jelly formulation, with a tendency to increase for both variables.
The formulation 5 (F5) is the sweetest and least acidic; however, the formulation F 1 is the least sweet and more acidic, of the tested formulations.
The processing of the jelly was done in good hygienic conditions, since no coliforms were found in the presumptive test.
In general, the formulation 5 (F 5 ) was more widely accepted among students, therefore, this is the ideal formulation to be used as an alternative in school meals for elementary school students in Fortaleza-CE municipal schools.

Table 1 -
Quantitative of the components in the formulations of the manipuçá jelly (Mouriri cearensis Huber).

Table 2 -
Physical and chemical characteristics of manipuçá jellies (Mouriri cearensis Huber).Averages followed by the same letter in line do not differ from each other by Tukey test at 5% probability of error. *