Effect of breed ( wool and hair ) and sex on the carcass quality of suckling lambs under intensive management

Carcasses of twenty lambs (10 males and 10 females) from the Canaria Hair breed (CHB) and another twenty (10 males and 10 females) from the Canaria breed (CB) of 9.91±0.73 kg and 9.87±0.80 kg live weight, respectively, were studied. In the principal component (PC) analysis, the projection of carcass measurement data in the first PC allowed for clearly distinguishing between CB and CHB. In the analysis of variance, breed effect was observed in the morphology of the carcass: CB lambs showed the longest carcasses and CHB lambs showed the widest carcasses. Due to their wider rump and shorter legs, CHB lambs presented a more compact leg index. The carcasses from Canaria lambs had the highest fat score, the heaviest shoulder and the highest total fat percentage. The carcasses from CHB lambs showed a higher lean percentage. The carcass yield, conformation, degree of fatness and tissue composition were similar in both sexes. However, significant differences in the commercial cut yield were observed: males showed heavier shoulders than females. The effect of sex on carcass quality is unimportant. The breeds are different in important characteristics for the market, such as fatness. This fact is of significant interest because it diversifies the offer and means that different markets can be satisfied.


Introduction
In the Canary Islands (Spain), three local sheep breeds are considered native, and qualify for special protection (R.D. 2129/2008): the Canaria (wool), the Palmera (wool) and the Canaria Hair (CHB).Although the population of these sheep increased by 51.57% between 2000 and 2010, the total number of animals was still only 80,304.In particular, the hair sheep population has increased in recent years.There are several reasons that explain the increase in CHB, the only hair breed in Europe (Bermejo et al., 2010): management requirements of hair breeds are simpler than those of wool breeds because they do not require shearing; their resistance to certain parasites, such as Haemonchus contortus (González et al., 2008); and hair breeds also show good productive performance in hot climates with high humidity (Camacho et al., 2010).These characteristics and the availability of agricultural by-products like feed (Camacho et al., 2011) make it an interesting breed for many areas such as southern Spain, where greenhouse farming generates large amounts of byproducts.Contrary to hair sheep, wool breeds (Canaria and Palmera) have reduced their numbers in the recent years, especially the Palmera breed (Hernández-Castellano, 2010).Although these breeds are used mainly for milk production, lamb meat is an interesting complement to the farm economy.
A deficit in sheep products is often observed in the Canary Islands.Sheep meat production was 1,591 t live weight in 2010, an amount lower than the demand (GOBCAN, 2010).Traditionally, the type of lamb produced is suckling lamb because of the large number of dairy sheep (wool) (Cañeque et al., 2004).Local breeds could be the basis of a meat production of certified quality (Protected Designation of Origin, Protected Geographical Indication or similar); however, the quality of sheep products must first be characterised and evaluated.For some authors, breed (Civit et al., 2009) and sex (Rodríguez et al., 2008) affect the carcass quality of suckling lambs Therefore, the objective of the present study is to evaluate the effect of breed and sex on the growth and carcass characteristics of Canaria (dairy) and Canaria Hair (meat) suckling lambs slaughtered at the same live weight.

Material and Methods
The present experiment complied with the norms of the Committee of Animal Research Ethics and Welfare of the University of La Laguna (R.D., 1202/2005; L., 14/2007).Twenty Canaria Hair (CHB) lamb carcasses (10 males and 10 females) and twenty Canaria (CB) lamb carcasses (10 males and 10 females) were studied.The animals were reared in the same conditions on a farm located in Tenerife (Canary Island, Spain) (28.07 N and 16.35 W), where the average annual temperature is 20.2 ºC and the relative humidity is 72%.Three rams were used in each breed group and the breeding period began simultaneously in all groups.The average prolificacy was similar in both breeds.
The lambs were weighed at birth and were identified with a numbered collar.During the study, they were kept with their dams.The lambs were individually weighed each week until reaching their slaughter weight (9.91±0.73kg live weight and 47.9 days for CHB and 9.87±0.80kg live weight and 51.1 days for CB).The lambs were reared following a traditional production system.During the first week of their life, all received vitamins A, D 3 and E and were vaccinated against enterotoxemia.The lambs received maternal milk until they reached 15 days of age.After day 15, milk-feeding was continued and free access to forage, concentrate (1,530 kcal of net energy and 18% crude protein) and water was initiated.Dams were fed ray-grass hay ad libitum, and a concentrate (1,638 kcal of net energy and 19% crude protein).When the lambs reached slaughter weight, they were transported to the slaughterhouse, according to EU regulations (Council Regulation (EEC) Nº 1 /2005, 2004).All of the animals were weighed twice, first at the farm, and then at the slaughterhouse, to identify losses due to transport.In the slaughterhouse, the animals were fasted (10-12 hours), and only water was provided (R.D. 54/1995).After measuring slaughter live weight (SLW), the lambs were electrically stunned and slaughtered according to standard commercial procedures.
The head, feet, skin, liver, heart, lung-trachea, spleen, thymus, testicle-penis, ovary-uterus and full and empty gastrointestinal tract of each lamb were weighed.The carcass included the kidneys, pelvic-renal fat and meaty portion of the diaphragm; however, the head (cut at occipitalatlantoidal articulation) and the limbs (cut at the carpometacarpal and tarso-metatarsal joints) were not included.The carcasses were weighed (hot carcass weight, HCW) and hung by the Achilles tendon after processing.Subsequently, the carcasses were cooled to 4 ºC for 24 hours.After chilling, the carcasses were weighed again (cold carcass weight, CCW), and the carcass yield (CCW/SLW × 100) and shrink losses (calculated as the difference between the HCW and CCW and expressed as a percentage of the HCW) were determined.
The data were analysed using SPSS (SPSS System, Inc., Chicago, USA, version 15.0).Principal components analysis was performed for carcass measurements (K, L, F, G, B and Wr variables) and tissue composition (L, TF, SF, IF and B variables).This analysis resulted in a graphical representation of the location of the observations in space made up of the functions obtained (component 1 and 2).The means were analysed by conducting an analysis of variance (ANOVA).The fixed effects of the model included the breed, sex and interaction effects of breed and sex.Differences among means were evaluated by conducting Scheffé's test.

Results
A principal component (PC) analysis performed on carcass measurements found that the first, two components explain 65.8% of total variation (Table 1).Maximum width at rib level (Wr), rump width (G) and leg length (F) were the measurements with highest values in the components matrix (0.862, 0.796 and -0.733, respectively).The two breeds are separated by 90º in the first component (Figure 1).The sexes are located in the four quadrants (Figure 2).
A Principal component analysis carried out on tissue composition of the carcass showed that the first two components explain 80% of total variation (Table 3).Total fat (TF) and subcutaneous fat (SF) were the variables with the highest values in the components matrix (0.929 and 0.864, respectively).There are no differences in the location of the breeds and sexes (Figure 3 and 4).The carcasses of CB lambs are located only in the positive zone of the first component (Figure 3).

Discussion
The two breeds are separated by 90º in the first component (Figure 1).Canaria-Hair-breed lambs were located mostly on the positive x-axis (component 1), because maximum width at rib level (Wr) and rump width (G) were the variables with the highest positive values.Therefore, CHB is the breed that produces best carcass quality because the carcasses are short, wide and compact and are, therefore, the more valued by the market (Diaz et al., 2006).There were no differences in the location of the breeds (Figures 3 and 4), regarding tissue composition.The carcasses of the CB lambs are located in the positive zone of the first component and only some carcasses of CHB are located in the negative zone of the first component.These correspond to carcasses with low lean and bone tissue, because these tissues showed high negative values in the components matrix (−0.761 and −0.750, respectively).
In the analysis of variance, breed did not have any effect on carcass yield in 10 kg lambs.This result agrees with Pérez et al. (2007), who evaluated four different pure and crossed breeds (Merino Precoz Aleman, Suffolk, Merino Precoz Aleman × Suffolk, and Suffolk × Corriedale), and demonstrated that the genotype did not affect the carcass yield at 10 kg slaughter weight.The carcass yield found by these authors was higher (52.6%) than that of CB and CHB lambs, perhaps because they are more specialised meat breeds.
Breed effect on carcass measurements was also observed by Miguélez et al. (2006) in suckling lambs of the Churra (dairy), Castellana (dairy-meat) and Ojalada (meat) breeds; and by Marino et al. (2008) in the Altamurana (dairy-meat) and Trimeticcio (meat) breeds.In agreement with Martínez-Cerezo et al. (2002), the CB lambs presented poor morphology for meat production (long carcass and narrow leg rump).Due to the wider rump and smaller leg length, CHB lambs presented carcasses with a more compact leg.Martínez-Cerezo et al. (2002) demonstrated that Churra lambs presented longer carcasses than the Rasa Aragonesa and Merino Español breeds, and these authors suggested that the morphology of the carcass in Churra lambs could be attributed to the dairy purpose of the breed.The differences observed in this study between breeds in carcass morphology could also be due to the different purposes of the breeds.
Canaria-breed lambs showed higher fatness scores.Differences between breeds in fatness have been attributed to differences in precocity or maturation of the breeds (Juárez et al. 2009).In the breed × sex interaction, it was observed that the higher fatness scores of CB were due to the high fat content of females.Díaz et al. (2006) also noted greater fatness in the females as a result of early maturing.Thus, the findings in relation to carcass fatness would indicate different precocity between breeds.
Canaria-breed lambs showed the highest shoulder percentage.Sanz et al. (2008), in Churra Tensina lambs, studying the evolution of different cuts during the growth period, conclude that the shoulder is a precocious joint, obtaining higher proportion at early ages.Thus, significant differences obtained in the proportion of second commercial cuts may be attributed to the precociousness of CB lambs.Canaria-Hair-breed lambs showed higher lean content, while CB lambs presented a higher total fat percentage.These results are in agreement with the results obtained in the subjective scoring of fatness (Table 1).Some authors have observed an increase in carcass fatness in dairy (Laborde et al., 2001) and precocious breeds (Ripoll et al., 2006).The higher total fat percentage of CB could, therefore, be due to their dairy purpose and precocity.
Sex did not affect any of the aforementioned variables (Table 2).Similarities in the growth rates of males and females explain these findings.Sex effect was only found in second-category joints.Male lambs showed the highest shoulder percentage.Díaz et al. (2006) obtained similar results and reported that male lambs presented greater development of neck and shoulder due to the response of certain muscles in these joints to the action of testosterone.
Lamb gender did not affect the tissue composition of the carcasses.In contrast, Díaz et al. (2006) observed significant differences between sexes in all tissues: males presented more bone and lean tissue than females, but less fat.These differences could be due to the fact that the slaughter age in this case was not the same between sexes.In this study, there were no differences in the slaughter age between males and females.
Gender did not affect L/TF, L/B or SF/IF ratios because males and females presented carcasses of similar tissue composition.Differences in the slaughter age between sexes could explain the results found by others authors, such as Santos et al. (2007), who observed, in suckling lambs, that there were significant differences between sexes in lean content and L/B ratio and demonstrated that females presented higher values.Similarly, Díaz et al. (2003) found that females presented higher L/B and SF/IF ratios and lower L/TF ratios than males because the females showed higher total fat and subcutaneous percentage and lower bone percentage.The PC analysis did not show gender differences in the measurements and tissue composition of the carcass.These results agree with the analysis of variance.

Conclusions
Despite the short life span of the lambs studied, there are significant differences between the breeds, which are important features for the market, such as fatness and carcass compactness.Although fatness is essential for maintaining moisture, consumers prefer meat with a lower fat content; therefore, hair breed carcasses are more suitable for Mediterranean markets than Canaria breed carcasses.

Figure 3 -
Figure 3 -Projection of the tissue composition data of the two breeds studied (Canaria Hair: ▲; Canaria: ○) in the plane defined by the two principal components.

Figure 4 -
Figure 4 -Projection of the tissue composition data of the two sexes (Male: ▲; Female: ○) in the plane defined by the two principal components.

Figure 2 -
Figure 2 -Projection of the carcass measurement data of the two sexes (Male: ▲; Female: ○) in the plane defined by two principal components.

Figure 1 -
Figure 1 -Projection of the carcass measurement data of the two breeds studied (Canaria: ▲; Canaria Hair: ○) in the plane defined by the two principal components.

Table 1 -
Results from the principal component analysis for the six principal components of the carcass measurements

Table 2 -
Means (±standard deviation) of carcass traits of suckling lambs (n = 40) of the Canaria and Canaria Hair breeds NS -non-significant (P>0.

Table 3 -
Results of the principal component analysis for the four principal components of the tissue composition

Table 4 -
Means (±standard deviation) of the different categories of joints, percentages and ratios of the main tissues of lamb carcasses (n = 40) of Canaria and Canaria Hair suckling lambs