Effects of tin caulis dendrobium polysaccharide on the prevention of non-alcoholic fatty liver disease in rats

We aimed to investigate the effects and mechanisms of tin caulis dendrobium polysaccharide on body weight and non-alcoholic fatty liver disease in Sprague-Dawleg rats. The rats were fed a high-fat diet (HFD) with or without tin caulis dendrobium polysaccharide for eight weeks. Body weight, liver function tests, serum and liver lipids, liver morphology, and the expression of PPARγ and UCP2 were assessed. Rats fed HFD exhibited more obvious weight gain, increased serum, and hepatic lipids. But the more tin caulis dendrobium polysaccharide they fed, the lower increase in weight, serum, and hepatic lipids of rats were found. Furthermore, the expression of PPARγ and UCP2 decreased in rats fed tin caulis dendrobium polysaccharide compared with HFD-fed rats. Tin caulis dendrobium polysaccharide may downregulate the expression of PPARγ and UCP2 in liver and protect against body weight gain and liver fat deposition.


Introduction
Non-alcoholic fatty liver disease (NAFLD) has been a disturbing clinical problem.In recent decades, the prevalence of NAFLD has significantly increased (Fan et al., 2005) and will replace viral hepatitis as the most common liver disease in China.Non-alcoholic fatty liver disease is closely related to obesity.Studies have found that fat metabolism in liver is associated with energy metabolism; many kinds of metabolites, hormones, cytokines, and neurotransmitters play an important part, such as tumor necrosis factor, leptin, and adiponectin.It is not only the result of dysbolism, but also the flare factor to normalize the liver fat metabolism.This course can change the activity of the above-mentioned regulators in other tissues and lead to insulin resistance, metabolic syndrome, tumors, and so on (Furukawa et al., 2004;Shiomi et al., 2013).
With the development of modern medical technology, many natural pigments from vegetables and fruits interest scientists and their non-toxicity and beneficial health effects attract people for prophylaxis or treatment, such as purified mulberry (Wu et al., 2013a), blueberry (Wu et al., 2013b), and resveratrol (Heeboll et al., 2015).Tin caulis dendrobium is widely used for antipyretic, immunomodulatory, and cardiovascular protection in traditional Chinese medicine, known as "Shihu" or "Fengdou".But whether tin caulis dendrobium can prevent the development of fatty liver disease is not clearly.In this study, we fed rats with tin caulis dendrobium polysaccharide and investigated its effect on the development of NAFLD.

Material and Methods
Ninety-five percent of dendrobium polysaccharide were obtained from Pro Tianhong Biotech Co., Ltd.(Hangzhou, P.R.China).Cholesterol, bile salts, egg yolk powder, edible lard, and the basal diet were purchased from Suyisi Biological Pharmaceutical Co., Ltd.(Jiangsu, P.R.China).Hematoxylin and eosin staining materials were purchased from Health Engineering Limited (Shanghai, P.R.China).
The experimental protocol was approved by the local Experimental Animal Ethics Committee (Case no.ZJU201308-1-10-072) and performed under the National Institutes of Health Guide for Care and Use of Laboratory Animals.
All animals were maintained under standard conditions of temperature (22±1 °C) and light (12 h light and 12 h dark cycle) and fed standard laboratory chow and tap water ad libitum.All procedures were in strict accordance with the PR China legislation on use.After 1-week adaptation, all experimental rats (n = 72) were randomly divided into six groups and fed specific diets for eight weeks.According to the daily feed intake, the groups included: the control group fed low-fat diet (LFD, 10% calories from fat); the control group fed LFD plus dendrobium polysaccharide (DEN, 500 mg per kg of feed); the control group fed a high-fat diet (HFD, 45% calories from fat); DL -the test group fed HFD plus low dose (250 mg) of DEN per kg of feed); DM -the test group fed HFD plus medium dose (500 mg) of DEN per kg of feed; and DH -the test group fed HFD plus high dose (1000 mg) of DEN per kg of feed.Body weight and the feed intake were measured once a week.
At week 8, blood samples were collected.After standing at room temperature, blood samples were centrifuged at 3000 revolutions/min for 15 min.Then, the serum was isolated and stored at −20 °C.Concentrations of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol (TC) were determined by biochemical auto analyzers.
At week 8, the rats were sacrificed by suffocation in nitrogen gas.After death, the abdominal cavity was opened and liver was isolated.Then liver was fixed with 4% paraformaldehyde for 2 h.Liver was washed in PBS three times (5 min at every turn).Specimens were embedded in paraffin, then cut (4-7-μm thickness), stained with hematoxylin/eosin, and examined under light microscopy.
Results were expressed as the means ± SEM (standard error of means).The statistical evaluation of the differences were compared between the different groups by one-way ANOVA.All data were processed and analyzed by SPSS13.0Data Editor (SPSS Inc., Chicago, IL, USA).The results in comparisons between groups were considered different if P<0.05.

Results
All rats fed HFD added with dendrobium polysaccharide for eight weeks were healthy.Initial average body weight of rats were 104.8 g, 105.5 g, and 106.7 g, which showed no significant difference from HFD group.After eight weeks, rats fed only HFD gained greater body weight and the weight growth rate was 280% (Table 1).Intake of DEN prevented body weight increase for the HFD-fed rats.High dose of DEN had the most significant effect, the effect of medium dose was the second most significant (P<0.05)(Table 1).
Rats fed LFD showed lower lipid accumulation (Figure 1A), while rats fed HFD showed intense lipid accumulation in the liver (Figure 1C).In contrast, rats fed HFD added with DEN had significantly alleviating lipid accumulation (Figure 1D-F).Furthermore, the protective action strength of DEN on rats was in direct ratio to the dosages of DEN.Group of medium and high dose of DEN showed morphology change similar to LFD group.
Serum ALT, AST, TG, and TC levels elevated compared with other groups.Rats fed DEN showed that the levels of serum parameters decreased slightly compared with HFD group.High doses of DEN significantly reduced AST by 12.3%, TC by 34.2%, and TG by 38.8% (P<0.05).Medium dose of DEN significantly reduced AST by 9.8%, TC by 33%, and TG by 38.9% (P<0.05).Low dose of DEN showed no significant effect on serum parameters (Table 2).
Relative mRNA expression levels of PPARγ and UCP2 in the liver increased in rats fed HFD compared with those fed LFD.Peroxisome proliferator-activated receptorgamma and UCP2 mRNA expression showed a significant decrease in rats fed HFD added with high dose and medium dose of DEN compared with HFD group, while similar to LFD group (Figure 2).

Discussion
With the development of modern medical technology, extraction of natural function factors from plants have interested scientists.As reported, dendrobium polysaccharide extracted from tin caulis dendrobium have biological effectiveness, such as cardiovascular protection and tumor inhibition (Gordon and Roedig-Penman, 1998).
However, it is unknown whether dendrobium polysaccharide can protect liver from non-alcoholic fatty liver disease.In this study, we fed rats a high-fat diet or low-fat diet, added with or without dendrobium polysaccharide, and observed the effect of dendrobium polysaccharide on obese rats.
Sprague-Dawleg rats are particularly sensitive to diet-induced obesity and are widely used in studies of metabolic disease, which is associated with obesity.As expected, in our study, the rats fed HFD had significant increase in body weight and serum analysis showed that glucose and lipid metabolism were affected, as well as the elevation of alanine aminotransferase and aspartate aminotransferase.These findings were in agreement with previous reports (Gordon and Roedig-Penman, 1998    On the other hand, diet added with different doses of dendrobium polysaccharide can reduce the increase of ALT, AST, TG, and TC levels in obese rats.The liver and adipose tissue analysis showed that dendrobium polysaccharide can also improve fat tissue hypertrophy and excessive fatty deposition in liver.Dendrobium polysaccharide showed the effect on preventing body weight and there was a positive correlation between the action strength and the dose of DEN.This result showed a dose-dependent manner (McClung et al., 2004).However, the mechanism of this increase tendency with the higher dose of DEN is still unclear.Peroxisome proliferator-activated receptors (PPAR) belong to ligand-activated transcription factors, a member of the metabolic nuclear receptor superfamily that regulate the activation of a variety of important target genes, and can be divided into three subtypes named PPARα, PPARβ, and PPARγ.The PPARγ-mediated target genes are closely associated with adipocyte differentiation, obesity, and insulin resistance (Vitale et al., 2016).During differentiation of preadipocytes and adipocytes, PPARγ is an important positive regulatory factor (Huan et al., 2003;Qi et al., 2015).On the other hand, the main part of body heat production is in the mitochondria and the quantity of heat depends on uncoupling protein (UCP) of mitochondria.In mammals, there are five types of uncoupling protein: UCP1, UCP2, UCP3, UCP4, and UCP5.The gene of UCP2 is located on human chromosome 11 and murine chromosome 7, associated with obesity (Zhang et al., 2014).Our study showed that the expression of PPARγ and UCP2 in liver fat tissue were significantly lower in obesity-resistant rats than in obesity-prone rats, which may be related to reducing steatosis, insulin resistance, and leptin resistance.It is a possible molecular mechanism of diet-induced obesity resistance (Steppan et al., 2001;Qi et al., 2015).

Conclusions
Dendrobium polysaccharide could significantly protect liver from fat deposition.Dendrobium polysaccharide may reduce the expression of PPARγ and UCP2, then improve lipid peroxidation and the development of obesity and fatty liver.Our results can provide the scientific base for intervention of nonalcoholic fatty liver by Dendrobium diet.Although the signaling pathway of how dendrobium polysaccharide effects on obesity is still unknown, dendrobium polysaccharide could be used to improve the development of non-alcoholic fatty liver disease in clinical.
ALT -alanine aminotransferase; AST -aspartate aminotransferase; TG -triglyceride; TC -total cholesterol; LFD -rats fed low-fat diet; DEN -rats fed LFD plus dendrobium polysaccharide (DEN, 500 mg per kg feed); HFD -rats fed high-fat diet; DL -rats fed HFD plus low dose (250 mg) of DEN per kg of feed; DM -rats fed HFD plus medium dose (500 mg) of DEN per kg of feed; DH -rats fed HFD plus high dose (1000 mg) of DEN per kg of feed.Different letters indicate a significant difference among columns (P<0.05).A: rats fed low-fat diet; B: rats fed low-fed diet plus dendrobium polysaccharide (500 mg per kg of feed); C: rats fed high-fat diet; D: rats fed high-fat diet plus low dose (250 mg) of dendrobium polysaccharide per kg of feed; E: rats fed high-fat diet plus medium dose (500 mg) of dendrobium polysaccharide per kg of feed; F: rats fed high-fat diet plus high dose (1000 mg) of dendrobium polysaccharide per kg of feed.