Available space in feeders for housed sheep : social behavior and performance

This study was designed to assess the effects of feeding space availability on social behavior and performance of housed crossbreed sheep through the use of three different types of feeders. Thirty-six crossbreed ewes (Santa Inês × Dorper; 2-3 years old) with initial body weight of 34.99±4.28 kg were randomly assigned to a 3 × 3 (feeder type × available space at the feeder) factorial arrangement among nine pens. Pens had one of three feeder types (concrete bunk, plastic, or wood barrel) and one of three amounts of available space in the feeder (0.33, 0.25, or 0.20 m animal−1). Total dry matter intake/pen, dry matter intake per animal, total weight gain/pen, daily weight gain/animal, and food waste were evaluated. Percentage of time spent at feeding and frequency of aggressive behaviors/12 h were recorded. We observed a higher total daily weight gain in ewes with a feeding space of 0.33 and 0.25 m animal−1. The wastes were essentially insignificant for wood feeders in all feeding space treatments. The average number of aggressive behaviors was lower (P< 0.05) for animals kept in pens with feeding space of 0.33 m animal−1 than for those maintained in pens with feeding space of 0.20 and 0.25 m animal−1. Ewes with feeding space of 0.33 m animal−1 fed simultaneously 54% of the time, while among pens with feeding spaces of 0.25 or 0.20 m animal−1, this period was reduced to 41 and 30%, respectively. An available feeding space of 0.33 m animal−1 is recommended for adult housed ewes, once it favors simultaneous access, lower competitive interactions at the feeder, and less variation in terms of individual weight gain among ewes.


Introduction
Brazilian livestock practices of domestic sheep (Ovis aries) can differ greatly, ranging from extensive pasture to intensive indoor system, differing in quantity and quality of space, which largely determine sheep behavior (Sevi et al., 1999;Estevez et al., 2007;Stafford and Gregory, 2008).Sufficient available space is essential to assure welfare of livestock species (Averós et al., 2014;Averós et al., 2016).
In confinement system, food can be a limiting resource when it is provided in a restricted way or when the available space in the feeder is not sufficient for all animals to feed simultaneously (Estevez et al., 2007).The effects of space restriction in feeders have been approached in broiler chickens and laying hens (Estevez et al., 1997;Hughes et al., 1997), dairy and beef cattle (Olofsson, 1999;Miranda-De La Lama et al., 2013), goats (Jorgensen et al., 2007; Van et al., 2007), and sheep (Henderson, 1985;Boe et al., 2006;Boe and Andersen, 2010).These surveys have reported changes in group behavior, increase in aggressive incidents, and reduction in feed intake and weight gain when evaluating animals with reduced feeding spaces.
In Brazilian semiarid region, goat and sheep farmers use feeders made of wood, plastic barrels, or concrete bunks.The affordable price of the materials is a determining factor for their choice (Barros et al., 2006;Silva et al., 2010).However, physical aspects, including feeding space, width, and depth should be considered, since they can affect feed waste (Martinson et al., 2012;Buskirk et al., 2003) and competition amongst animals.Feeder recommendations may vary according to breed, sex, and age of animal, and type of food or supplement that is offered (Arnold and Maller, 1974;Ítavo et al., 2009;Jorgensen et al., 2007;Boe and Andersen, 2010;Averós et al., 2014).Ítavo et al. (2009) suggested a feeding space of 0.10 to 0.15 m animal −1 for adult sheep in confined areas to allow simultaneous access for all animals.Boe and Andersen (2010) recommended 0.35 to 0.45 m animal −1 as the ideal space for housed ewes; Stevens et al. (2005) suggested 0.30 m of depth for adult sheep feeders.
The effects of available space and other physical characteristics of the feeders on social behavior and performance of housed sheep are topics that should be better investigated.Thus, the objective of this study was to assess the effects of feeding space availability on social behavior and performance of housed crossbreed sheep through the use of three types of feeders (wood, plastic barrel, and concrete bunk).We had predicted that a reduction of feeding space would increase competitive interactions among sheep and amount of feed waste in the feeders; ewes could have difficulties to feed simultaneously when the space in the feeder was less than 0.33 m animal −1 .Moreover, feeders of concrete bunk and plastic barrel would favor greater food waste and competitive interactions among ewes.Results of this study have valuable implications on the management of housed sheep, especially regarding animal welfare and economic efficiency.

Material and Methods
The study was carried out in São João do Cariri, PB, Brasil (7°23' S, 36°32' W, 449 m altitude).Thirtysix crossbreed ewes (Santa Inês × Dorper; 2-3 years old) with initial body weight of 34.99±4.28kg were placed in confinement from November 27, 2013 to January 12, 2014.The animals were randomly assigned to a 3 × 3 factorial arrangement (feeder type × available space at the feeder).Before the beginning of the experimental phase, ewes were subjected to an adaptation and handling period at the facility for ten days.Nine pens were used, each containing one of three feeder types (concrete bunk, plastic barrel, or wood) and three, four, or five ewes with fixed stocking rate of 4.1 m 2 animal −1 for all pens.
Pens were built on compacted soil and had an area covered with clay tile (3.75 m 2 , 4.95 m 2 , and 6.15 m 2 for pens with three, four, and five ewes, respectively).Pens contained drinkers made from tractor tires (cut in half) and were separated with wood posts and galvanized wire mesh.The feeders had the following dimensions: 1 × 0.30 × 0.30 m for wood feeders, 1 × 0.30 × 0.17 m for concrete bunks, and 1 × 0.30 × 0.16 m for plastic barrels (Figure 1).Therefore, the amounts of space in the feeders were 0.33, 0.25, and 0.20 m animal −1 in pens with three, four, and five ewes, respectively (Figure 2).Except for the wood, models of feeder were similar to those used by sheep and goat farmers in the semiarid region of Brazil (Silva et al., 2010).We tried to represent a recommended design of wood feeder based on Stevens et al. (2005).
Food was offered ad libitum both at 06:30 and 12:30 h, and intake was regulated to allow 20% food residue; water was also provided ad libitum.The experimental diet was formulated to meet the requirements of adult sheep in the breeding season for a daily gain of 150 g animal −1 (NRC, 1985).The roughage:concentrate ratio was 50:50, and these were offered as total mixed ration (Tifton hay, corn meal, soy and wheat, and mineral and vitamin supplementation).Leftovers in the feeders and part of the feed that fell on the ground were removed and weighed on an electronic balance for waste (%) estimations, being determined as the ratio between the leftovers collected outside the feeder and total residues.A 2.0 × 2.0-m rubber mat was placed on the ground under the feeder, which reached approximately 0.5 m around the feeder (front and sides).The food that fell on the ground and surrounded the feeder was collected daily at 06:00 and 12:00 h.
Initial and final body weights were determined (kg animal −1 ) in the beginning and end of the confinement period, respectively.Total dry matter intake (kg pen day −1 ), estimation of individual dry matter intake (kg animal day −1 ), total weight gain (kg animal −1 ), and daily weight gain (g animal day −1 ) were quantified.Total dry matter intake was obtained by subtracting collected food residues from the amount of offered food daily, in each pen; individual dry matter intake was estimated from the ratio between total intake and the number of animals in each pen; total weight gain was calculated as the difference between the final and initial weights of the animals; and daily weight gain was obtained from the ratio between total weight gain and number of days that animals were confined.
Behavioral observations were performed directly and continuously by focal animal sampling, from 06:00 to 18:00 h (Martin and Bateson, 1986).Ewes were individually tagged with non-toxic dye (ORBI-6694, Brazil) on both sides for identification.Nine observations were made in the initial (December 7, 8, and 9), middle (December 17, 18, and 19), and final (January 6, 11, and 12) confinement phases, totalizing 108 h of records.Platforms that were 4 m above the ground were used to observe the animals from 6 m away.Observations were performed by twelve trained people.Before the beginning of the experiment, all observers were subjected to a reliability test.
In this test, observers watched videos of the animals that were taken during adaptation period and recorded animal activities.At the end of the test, the records from all observers were compared.The procedure was repeated until a correlation of at least 95% between observations were obtained (Fonsêca et al., 2014).Recorded behaviors included percentage of time that ewes spent feeding in each observation and frequency of agonistic behaviors (calculated as number of times or incidents/12h), which comprised physical, passive displacements, butting, and frontal clashing (Table 1).All behaviors were recorded when animals were at the feeder area.
The normality distribution was checked by examining skewness and kurtosis coefficients.Thus, a general linear model (GLM proc.) was used to test the fixed effects (space available and type of feeder) on ewe performance and food waste, using Tukey's test to verify differences between treatments following the model:

Frontal position
Ewe with the body axis directed perpendicularly to the feeder.

Lateral position
Ewe with the body axis directed parallel to the feeder.

Agressive behaviors
Physical displacements at the feeder Ewe is forced to leave the feed barrier because another ewe is butting or pushing it.Passive displacement at the feeder Ewe leaves the feed barrier because another ewe is approaching it, but without physical contact.

Frontal clashing
The agent is rearing onto the hind legs with the head and torso twisted followed by descending forcefully onto the hind legs delivering a powerful strike forward and downwards reaching the head of the receiver.

Butting
Head towards the head or shoulders of another ewe.
maximum likelihood (Boykin et al., 2010;Fonsêca et al., 2016) to examine the effects of different types of feeders and available space in the feeders on animal behavior.Mean value per group was used as statistical unit.We used a lognormal response distribution for data recorded as time (time spent feeding) and negative binomial for frequency (aggressive behaviors) data.All analyses were performed using SAS (Statistical Analysis System, version 9.3) statistical software.

Results
Interaction between feeding space and feeder type for performance data was not observed.Initial and final body weights of the sheep, as well as total and individual dry matter intake, were similar across all feeder types (all P>0.05) (Table 2).Higher and lower final body weights were observed in ewes with feeding spaces of 0.33 and 0.20 m animal −1 , respectively.Dispersion was greater (CV, %) among groups with less available space in the feeder (Table 3).We observed a higher total daily weight gain in ewes with a feeding space of 0.33 and 0.25 m animal −1 .Additionally, weight gain variation of ewes in pens with feeding space of 0.20 m animal −1 was more pronounced, as it was seen by the higher coefficient of variation.Animals with a greater amount of available feeding space (0.33 m animal −1 ) consumed more (P<0.05)food.
The amount of total residue was the same in all pens, no matter the feeder type (P = 0.1542; F (2.20) = 1.32) or amount of feeding space (P = 0.5212; F (2.32) = 1.01).Total daily residue ranged from 0.823 to 1.010 kg/pen.On the other hand, greater percentage of waste was observed in concrete feeders followed by plastic barrel feeders (P = 0.0001; F (2.18) = 4.32) (Table 4).Waste was essentially negligible for wood feeders in all feeding space treatments.Furthermore, when feeding space was reduced in pens with concrete and plastic barrel feeders, there was a largest quantity of food waste (P = 0.0050; F (2.78) = 3.38).
Neither the type of feeder (P = 0.1011; F (0.83) = 0.30) nor the amount of feeding space (P = 0.1450; F (0.90) = 0.45) influenced the time spent at feeding.The average percentage of time spent at feeding was 29.33% (2.9 h), 32.66% (3.2 h), and 29.66% (2.96 h) for treatments with feeding space of 0.33, 0.25, and 0.20 m animal −1 , respectively.However, animals with feeding space of 0.33 m animal −1 fed simultaneously 54% of the time, while this period was reduced to 41 and 30% in groups with feeding space of 0.25 and 0.20 m animal −1 , on average (Table 5).
Interaction between feeder type and available space was evidenced through aggressive behaviors (P = 0.0234;  F (0.92) = 2.64 (Figure 3).The average number of aggressive behaviors was lower (P = 0.0123; F (0.88) = 2.30) for animals kept in pens with feeding space of 0.33 m animal −1 than for those maintained in pens with feeding space of 0.20 and 0.25 m animal −1 .In pens with plastic barrel feeders, the frequency of aggressive behaviors was greater in groups with feeding space of 0.33 m animal −1 than in groups with 0.20 m animal −1 .In pens with concrete feeders, ewes competed more when feeding space was decreased to 0.25 and 0.20 m animal −1 .

Discussion
The objective of this work was to assess the effects of feeding space availability on social behavior and performance of crossbreed housed sheep with the use of three types of feeders (wood, plastic barrel, and concrete bunk).As we predicted, space availability in feeders made of concrete, plastic barrel, and wood influenced social behavior and performance of housed ewes.Furthermore, the design of feeders affected the frequency of aggressive behaviors among ewes and the amount of food waste.Based on our results, feeders with feeding space smaller than 0.33 m animal −1 led to higher levels of aggressive behaviors and lower time spent at simultaneous feeding for all animals.When the space in feeders was decreased to 0.20 m animal −1 , a greater variation in terms of individual weight gain and final weight and overall decline in performance were observed among animals.Moreover, a minimum percentage of food waste was found in wood feeder, considering all the feeding spaces tested.
Surveys with farmed species have previously documented increases in frequencies of aggressive behaviors, changes in the amount of ingested food, and a decline in performance among animals when space in feeders was reduced (sheep: Henderson, 1985;Boe and Andersen, 2010;goats: Loretz et al., 2004;Jorgensen et al., 2007;cattle: DeVries et al., 2004;Miranda et al., 2013).In contrast with these findings, the design and amount of space in feeders of this study did not affect the time that ewes spent at feeding.For instance, Boe and Andersen (2010) verified that time housed ewes spent at feeding declined approximately 27.5% when space in the feeder decreased.The average percentage of time spent at feeding was 29.33 (2.9 h), 32.66 (3.2 h), and 29.66% (2.96 h) for treatments with feeding spaces of 0.33, 0.25, and 0.20 m animal −1 , respectively.
In a competitive situation, animals of lower rank may prefer to feed when others are not, thus avoiding consequences of agonistic encounters when food is given.Shinde et al. (2004) and Olofsson (1999) reported that the easiest way to maintain feeding time when feeding space is reduced would be to consume the feed at different times throughout the day.Certainly, this may explain the results of the present investigation.Such changes could be inferred from the percentage of time that all animals fed simultaneously.According to our findings, animals with feeding space of 0.33 m animal −1 fed simultaneously 54% of the time.This percentage decreased to 41 and 30% in groups with feeding spaces of 0.25 and 0.20 m animal −1 ,    respectively.Similarly, in studies with dairy cattle, Friend and Polan (1974) and DeVries et al. (2004) also observed an increase in simultaneous feeding time when more space was offered in the feeder line.The results of this study do not support the technical recommendation of Ítavo et al. (2009), who suggested that a feeding space for adult sheep ranging from 0.10 to 0.15 m −1 is sufficient to allow simultaneous access by all animals.Conversely, the results are consistent with the findings of Boe and Andersen (2010), which recommended 0.35 to 0.45 m animal −1 as the ideal feeding space for housed ewes.
In the moment of offering feed, animals that have prior access tend to select more digestible and nutritious parts, while subjects that prefer to feed at other times can ingest insufficient amounts and more indigestible portions of food (Shinde et al., 2004), resulting in lower and more variable performance in the group.In a work with housed dairy goats, Jorgensen et al. (2007) observed that lowstatus animals spent less percentage of total observations feeding and more percentage of total observations queuing than high-status goats.This effect was more evident as the feeding space was restricted.In agreement, our findings showed lower and more variable weight gain among ewes when space in the feeder decreased from 0.33 m animal −1 to 0.20 m animal −1 .It is likely that dominant animals had prior access to the feeder at the time of offering feed.Van et al. (2007) stated that the coefficient of variation for final weights of lambs and goats were higher under spacerestricted conditions in the pen.
When enough space is provided in the feeder, animals avoid violating individual space, which decreases aggressive interactions.In this study, ewes competed less when they had more space in the feeders.Our results indicated that the average number of aggressive behaviors was lower for animals kept in pens with feeding space of 0.33 m animal −1 than for those maintained in pens with feeding space of 0.20 and 0.25 m animal −1 .The increase in agonistic interactions on feeding in space-restricted conditions is the main cause of changes in the amount of ingested food in some animals in the group (Estevez et al., 2007).Previous findings with ewes and goats have also demonstrated an increase in aggressive interactions when space availability in feeders and resting areas was decreased (Lynch et al., 1989;Boe et al., 2006;Jorgensen et al., 2007;Boe and Andersen, 2010).
Besides the feeding space effect on social behavior and performance of confined ewes, results of this work also showed that feeder design led to differences in the frequency of aggressive behaviors and percentage of food waste.As we hypothesized, when feeding space was reduced in pens with concrete and plastic barrel feeders, there was a great amount of food waste and aggressive interactions.Tested feeders were manufactured from wood, concrete bunks, and plastic barrel.The main difference between them lies in the depth.Wood feeder had 0.30 m against 0.16 and 0.17 m for concrete bunk and plastic barrel, respectively.Our findings showed that higher frequency of aggressive behaviors and greater percentage of waste were observed at feeders made of concrete followed by plastic barrels, especially when feeding space was reduced.On the other hand, even under reduced feeding space, wood feeders led to practically no food waste and lower levels of aggressive interactions.Daily percentage of waste ranged from 35 to 46% for plastic barrel feeders and 45 to 70% for pens with concrete feeders, on average.In absolute terms, considering a mean daily waste of 0.500 kg/pen, total losses would reach 180 kg of food at the end of the confinement period.
This is a relevant concern, because it directly affects payback time and, consequently, the economic efficiency of the system.Obviously, a refinement in the cost-benefit ratio of each feeder needs to be included in the economic analyses.For this, longevity and purchase price of feeders should be accounted.Presently, we are unaware of any study on housed sheep that has specifically examined the effects of different feeder designs on behavioral patterns and percentage of feed waste.Previous investigations determined hay waste and economics of nine round-bale feeder designs used in horse feeding (Martinson et al., 2012).According to these authors, hay waste differed between round-bale feeder designs.Feeders designed to provide greater physical restrictions resulted in less hay waste compared with feeders that provided easier access to hay.Results of the present study certainly should be attributed to the physical characteristics of the tested feeders, knowing that the depth of wood feeder contributed to lower waste percentage.Thus, such investigation support technical recommendation made by Stevens et al. (2005), who suggested a depth of 0.30 m for feeders for adult sheep in confinement.

Conclusions
An available feeding space of 0.33 m animal −1 is recommended for adult housed ewes, once it favors simultaneous access, lower competitive interactions at the feeder, and less variation in terms of individual weight gain among ewes.
Food waste differs among the tested feeders.Nevertheless, we suggest that plastic barrel, concrete, and wood feeders can be used in confinement systems for adult sheep, as long as space and depth requirements are met.

Figure 2 -
Figure 2 -Layout of the experimental treatments.

Figure 3 -
Figure 3 -Frequency of aggressive behaviors (number of times/10 h) in the experimental treatments.

Table 1 -
Behavioral categories

Table 2 -
Performance of ewes (±SD) fed in wood, concrete bunk, and plastic barrel feeders i -individual; t -total.

Table 3 -
Performance of ewes (±SD) in the different feeding space treatment SD -standard deviation; CV -coefficient of variation; i -individual; t -total.Values with different letters differ significantly (P<0.05).

Table 5 -
Number of ewes feeding at the same time (mean±SE; % of total observation) SE -standard error.

Table 4 -
Estimation of feed waste (%) as a function of feeder type and available space (mean±SE) Percentage of waste was determined as the ratio of the leftovers collected outside the feeder and the total residuals. 1