UNRAVELING PHYSIOLOGICAL TRAITS OF Jatropha curcas, A BIODIESEL PLANT, TO OVERCOME SALINITY CONDITIONS

Dry land systems spread all over the world and comprise 41.3% of the terrestrial area, which host 34.7% of the global population, so it is convenient to propose crops able to grow there. Jatropha curcas is a plant adapted to arid and semiarid regions as well as sub-humid conditions, being a potential source of biodiesel. The challenge is to understand the physiology of J. curcas, which enables it to live under saline and drought conditions. The seeds of J. curcas used came from Ciego de Ávila Province, Cuba. Seven-day-old seedlings were cultivated in 1.5 L pots with half strength Hoagland solution for 42 days under semi-controlled conditions. NaCl added to solutions in pots provided 75 or 150 mM treatments for 240 h before measurements. Leaf growth, net photosynthesis and stomatal pore area were affected by 150 mM NaCl. Non-photochemical quenching of leaves was only changed by 150 mM NaCl after 24 h; the electron transport rate had a tendency to decrease in leaves under saline conditions. The gene expression pattern changed for SOS1 and HKT1 according to the NaCl used in the medium, indicating active mechanism to deal with Na in the cell. In general, Cuban J. curcas plants were able to grow and perform photosynthesis under 75 mM NaCl, which represents 7 dS m, a condition that restricts growth for many plant species.


INTRODUCTION
It is estimated that approximately 20% of the cultivated land in the world and almost half of all irrigated land is affected by salinity (SHIRIVASTAVA; KUMAR, 2015). Soil salinity is a serious environmental problem especially in arid and semiarid areas (ALLBED; KUMAR, 2013), where evapotranspiration rate generally exceeds rainfall. Salt stress interferes with plant growth, changing metabolic, physiological and anatomical features, which contributes to the reduction of crop yield and causes enormous economic losses. High concentrations of Na + and Clions in leaves could affect the stomatal and non-stomatal components of photosynthesis. The accumulation of intracellular Na + during salt stress conditions could change the K + /Na + ratio, which seems to affect the photosynthetic process (BRUGNOLI; BJÖRKMAN, 1992).
Under environmental conditions that are unfavorable for the photosynthetic fixation of CO 2 , an excess of light energy absorbed by photosynthetic pigments accelerates photoinhibition; however, plants exhibit different strategies to protect themselves against photoinhibition and photosynthetic damage (TAKAHASHI; BADGER, 2011).
Jatropha curcas L. has been considered a promising agro-energy crop, mainly because it is a very resistant oilseed plant that grows naturally in non-cultivable areas and is often exposed to drought conditions (SAPETA et al., 2013), heavy metals (RAJ; MOHAN, 2016), high temperatures (SILVA et al., 2018) and salinity (DÍAZ-LÓPEZ et al., 2012). For this reason, the survival capacity of J. curcas L. plants to abiotic stress has been the subject of previous studies. Despite these studies, there have been few reports on the photosynthetic responses of J. curcas L. plant to salinity. In the present study, we analyzed the photosynthetic and respiratory metabolism of J. curcas L. exposed to salt stress conditions.
The quantification of gas exchange alone is not enough to conclude about the effect of salinity on the physiological response of a plant. However, if it is combined with other parameters like growth, membrane damage, electron flux and gene expression, all together could be a good tool to quantify the tolerance to different stress conditions. In addition, the capacity of some plants to modify the expression of genes involved in cell homeostasis, especially those including SOS family of Na + /H + exchangers and HKT, a Na + transporter, has been a key step to understanding the salt tolerance process.
Modification of the expression pattern of SOS genes was analyzed in this work.

Plant growth conditions
Seeds of J. curcas L. plants growing wild in the eastern-central region of Cuba (Ciego de Ávila province) were previously selected by size (2 ± 0.1 cm large) and were surface disinfected using commercial hypochlorite (0.5%, V/V) for 5 min. The disinfectant was removed by at least five washings with sterile distilled water for at least 1 min each.
Seeds germinated in paper towel roll system with a ratio of 1.5 mL of water per gram of paper. Seedlings of about 7 days old were transferred to 1.5 L pots containing Hoagland nutrient solution (HOAGLAND; ARNON, 1950), half strength, pH 6.0, where air was pumped to provide the required conditions for the respiration of the root system. The hydroponic cultivation was set under semi-controlled conditions (temperature: 28±1.5 °C, illumination: 100 µmol photons m -2 s -1 , 65% RH, 12h light/12h dark cycle).
Stress conditions were applied to 42-day-old plants. Two sets of plants stayed in half-strength Hoagland nutrient solution supplied with 75 or 150 mM NaCl for 10 days. One set of plants remained in half-strength Hoagland nutrient solution as control.

Growth rate and membrane damage
Relative Leaf Growth Rates (RLGR) and Absolute Leaf Growth Rates (ALGR) were determined by non-destructive analyses, using the equations: RLGR [cm 2 cm -2 day -1 ] = (Ln A 2 -Ln A 1 ) / (t 2 -t 1 ); ALGR [cm 2 day -1 ] = (A 2 -A 1 )/ (t 2 -t 1 ) according to Hunt (1990); A 2 represents the leaf area at t 2 and A 1 the leaf area at t 1 . Pictures of the leaf were taken with a commercial camera (Canon EOS 700D, 5184 x 3456) using a cardboard with scale as background for each leaf. The leaf area was determined using the images of all leaves and analyzed by ImageJ software (version 1.45).
The relative water content (RWC) was determined following Irigoyen, Emerich and Sanchez-Diaz (1992), calculated as RWC = (fresh weight -dry weight)/(turgid weight -dry weight). The complete leaf was weighed immediately after harvesting to obtain the fresh weight (FW). The same tissues were placed in water at 25 °C for 6 h, followed by weighing, and the turgid weights were calculated. The samples were dried in an oven at 75 ° C until constant weight to obtain dry weights.
Ion leakage, an indirect method for assessing damage to cell membrane was used. The selected leaves or roots were washed with water. The leaf was cut into discs of 2.5 cm diameter and the roots were cut into pieces of similar size and transferred to 100-mL flasks containing 25 mL of bidistilled sterile water at 25 °C. The flask was shaken on a gyratory shaker at 250 rpm for 4 h at RT. After incubation, initial electrical conductivity (Ci) was measured using a Hanna HI8633 conductivity meter. To obtain a reference value for the conductivity, the samples were boiled for 5 min and the final electrical conductivity (Cf) was measured. The leaf ion leakage was calculated as (Ci/Cf)*100 (GULEN; ERIS, 2004).

Gas exchange and chlorophyll fluorescence
To determine the quantity of stomata and stomatal opening, the methodology described by impressions and collection, with a clear tape, of the epidermal surface was applied (SLAVIK, 1974). Epidermal impressions of the abaxial and adaxial surfaces of leaves were taken. Samples were observed and photographed under an Olympus BX-40 optical microscope, coupled to a DP-70 camera using Stream Essentials software (v1.6.1.).
The photosynthetic and respiratory activity of plants was determined in the youngest fullyexpanded leaves attached to the plants (about 52 days old) using an open-system infrared gas analyzer (IRGA) (Qubit Systems Inc., Canada) with 200 mL min -1 air flux. Photosynthetic activity was measured at saturating light intensity of 620 µmol m -2 s -1 (PAR). Respiration measurements were done in the dark. Chlorophyll fluorescence was measured using modulated light fluorimeter (MINI-PAM) (Walz, Germany). Basic (background) chlorophyll fluorescence (Fo), maximal chlorophyll fluorescence (Fm), maximum quantum yield of PS II (Fv/Fm), PSII efficiency in light (Fv'/Fm'), photochemical quenching (qP), non-photochemical quenching (qN) and relative electron transport rate (ETR) were quantified.
The leaves were previously adapted to the dark for 30 minutes, enabling all PS II reaction centers and electron carriers of the PS II acceptor side to be re-oxidized. Fo was obtained with low intensity modulated light (<0.1 mmol m -2 s -1 ) to avoid any effect on the variable fluorescence. Fm was obtained by 0.6 pulses of saturating light at 10000 µmol m -2 s -1 . Fv was calculated from the difference Fm-Fo. Fm', Fv' and Fo' values were obtained in the above samples with application of actinic light of 190 µmol m -2 s -1 and pulses of saturating light at 10000 µmol m -2 s -1 . The air temperature and humidity during the experiment was about 28 °C and 59%, respectively.

RNA Isolation and Quantification by RT-PCR
Total RNA was isolated using a Plant RNA Kit (Analytic Jena, Jena, Germany). Equal loading of RNA was visualized by EtBr stained rRNA. Aliquots of 2 μg RNA were treated with DNaseI prior to the reverse transcription reaction using RevertAidTM Reverse Transcriptase (Fermentas, Life Sciences) with Oligo(dT)18.
For semi-quantitative RT-PCR an equal concentration of cDNA (relative to JcActin, a housekeeping gene) was used for specific primer amplification. cDNA products were amplified with 0.5 unit of Dream Taq Green Polymerase (Fermentas) in the buffer provided by the manufacturer, which contains MgCl 2 , in the presence of the specific oligonucleotides and dNTPs. Reactions were carried out in the T3Thermocycler PCR system. A first denaturation step of 5 min at 95 °C was followed by a number of specific cycles of 30 s at 95 °C, 30 s at specific annealing temperature (according to the primer) and 30 s at 72 °C, followed by a final extension for 5 min at 72 °C.

Statistical analysis
The data met the criteria of normal distribution (chi-square) and homogeneity of variances (Levene Test) and were analyzed by parametric tests. The type of analysis of variance (ANOVA) used depended on the experimental design. One-way ANOVA was used to analyze designs with a single categorical independent variable (factor) and factorial ANOVA was used to analyze the higher order interactive effects of multiple categorical independent variables. The Range test (multi-stage) was performed using Tukey tests. When the results of statistical analysis were similar for α = 0.05 and α = 0.01, only difference for α = 0.01 is informed; if they were different, only α = 0.05 is informed. Data analysis was conducted using STATISTICA (data analysis software system), version 7 (www.statsoft.com) (StatSoft, Inc., 2004).

Membrane damage
The cell membrane of roots was affected within the first 6 h of 75 and 150 mM NaCl treatment ( Figure 1). Although differences in the percentage of ion leakage from stress treatments relative to control increased further after 240 h, the mean values of the percentage of electrolyte leakage decreased compared to the first 6 h of stress. Ion leakage in the leaf was not affected (data not shown). Electrolyte leakage is an important permeability index of the cell membrane. In the present study, the increase in electrolyte leakage suggests increases in membrane permeability, possibly due to the intensification of lipid peroxidation, common in plants subjected to salinity (JIANG et al., 2017). Nevertheless, an apoplastic accumulation of ions in salt-stressed samples will contribute to electrolyte leakage although they are not involved in cellular efflux. In any of the cases, the decrease of ion leakage as stress time increased could be an indication of less damage or less ion accumulation in the apoplast. This is evidence that some tolerant mechanism is functioning.

Plant growth and water content
The rates of leaf growth, represented by RLGR and ALGR, decreased after 240 h of exposure to salt stress in the treatment with 150 mM NaCl (Table 1). 1 The ALGR and RLGR of J. curcas L. leaves under salt treatment of 75 mM NaCl showed a similar response to those found in the control, indicating an acclimation to the saline conditions after 240 h. The reduction of ALGR and RLGR in plants growing under 150 mM of NaCl compared to control can be associated with senescence and leaf abscission processes. These two processes are caused by an excess of toxic ions such as Na + and Cl - (MUNNS, 2002). Díaz-López et al. (2012) reported an increase of Na + and Clin the leaves and roots of J. curcas L. growing under saline conditions for 40 days.
The presence of NaCl in the nutrient solution reduced the RWC in leaves of J. curcas for all exposure times under the highest salinity condition (150 mM NaCl), with significant differences compared to the control treatment level. Relative water content of plants with NaCl concentrations of 75 mM did not differ from those of the control and were different only from the higher salinity treatment at 24 h ( Figure 2). The lowest RWC values were obtained in the treatment of 150 mM. The reduction in RWC compared to control was not extremely strong because it remained between 93.33% and 89.39%; nevertheless, it could have an influence on plant growth and other physiological processes. The rate of irreversible cell volume increase is proportional to the rates of water uptake by the expanding cell. Thus, plant cell expansion depends on turgor pressure in a direct manner as well as on biochemical processes acting on the wall structure, such as increase in the activity of specific enzymes (MOORE et al. 2008). According to Rad et al. (2013), the RWC can be used as parameter to identify tolerant genotypes with high yield. In our case the lowest value of RWC (89.39%) obtained under the highest salinity stress (150 mM NaCl) is considered according to Moore et al. (2008) a mild degree of water deficit. Other effects of water deficit are modification in the synthesis and transport of growth regulators, stomatal behavior, respiration, photosynthesis, nutrient uptake, among others (BRADFORD; HSIAO, 1982). The presence of NaCl in the nutrient solution reduced the RWC of J. curcas L. leaf after 6 h of salt treatment (75 mM NaCl) and after 6 and 24 h of 150 mM NaCl ( Figure  2). The soil-root osmotic gradient is the main force responsible for water absorption by the plant. In saline soil the osmotic contribution to water retention is high, causing reduced water availability, hence reducing the absorption of water by the roots. Negative correlation between total water uptake and salinity has been reported by Pessarakli, Huber and tucker (1989). Under salt stress the plant root permeability (expressed as hydraulic conductivity of the root system) decreased significantly. This may explain the reduction in water absorption rate under salt stress conditions.

Rev
The cells appear turgid in control plants and even in the 75 mM NaCl treatment; in 150 mM NaCl treatment cells appear like wrinkled cells, especially observed after 24 h (Figure 3). Possibly, the loss of cellular turgor observed in leaf epidermal cells of plants exposed to 150 mM NaCl during the first 24 h may also be associated with a reduction in RWC because this phenomenon is directly related to the declining availability of water, which leads to a loss of cell turgor. It is made clear with the appearance of wrinkled cells (W) observed in the leaves of J. curcas L. plants after 24 h of saline treatment (Figure 3 B).
The low variations in the RWC of Jatropha curcas L., under 75 mM NaCl, corroborate studies by Díaz-López et al. (2012) that prove the species' moderate tolerance to salinity of up to 90 mM NaCl. The authors also suggest that the effects of ionic stress prevail over the effects of the physiological drought of salt stress, as evidenced by the low variation of RWC in the same study.
According to Wang and Nii (2015), the leaf area did not increase during the period of salinity stress in Amaranthus plants. However, salinity caused a reduction in the volume of epidermal cells in J. curcas leaves, especially in plants receiving the treatment of 150 mM NaCl after 24 hours of exposure (Figure 3 B). This volume reduction could be due to a reduction in cell turgor pressure, provoked by the osmotic effect of NaCl in the growing medium. This effect probably has a direct influence on the reduction of leaf growth rate, since the plant growth is the consequence of cell division and cell elongation. As leaves lose water, the cellular turgor pressure decreases and the cell membrane separates from the cell wall. After a critical point, the cell deformity results in a shrinkage of the leaf (KRAMER; BOYER, 1995) provoking leaf area reduction. Leaf initiation, which is governed by cell division, was shown to be unaffected by salt stress in sugar beet, but leaf extension was found to be a salt-sensitive process (BATOOL; SHAHZAD; NOSHIN, 2014). At the plant level, leaf expansion is one of the most sensitive processes to water stress (BOYER, 1970), one of the consequences of salt stress. Tardieu, Granier and Muller (2011) proposed a network of coordination for processes involved in the decrease of growth with water deficit. Three essentially independent macroscopic processes are affected in parallel, biomass accumulation, cell division and expansive growth, without central coordination with feedback between them.
A reduction in leaf area can be related to a strategy to decrease transpiration and thus to conserve the available water. Reduced leaf area is probably the more obvious mechanism by which the plants restrict the water loss in response to drought (CONNOR; JONES, 1985). However, the lower RLGR and ALGR values obtained after 240 h could be a result of the lower photosynthesis, influencing the growth of the plant. It may have been influenced by the limitation of CO 2 assimilation that occurs in plants under this condition.

Gas exchange
Net photosynthesis decreased significantly in the leaves of plants growing under the salt conditions of 150 mM NaCl after 6 h of exposure and did not recover after 240 h (Figure 4). The photosynthesis in plants maintained at 75 mM NaCl concentration was similar to that of control plants for the three exposure times. Respiratory activity was lower in plants in the presence of NaCl 75 and 150 mM NaCl relative to the control for the first 6 hours, but maintained similar values to those of control plants after 24 and 240 h (Figure 4). The extreme photosynthetic dysfunction observed in plants under 150 mM NaCl can be induced by different causes at different levels: a low capacity of photochemical reactions that lead to loss of efficiency in the transformation of light energy, a low CO 2 diffusion into the intercellular spaces due to decrease in the size of stomatal opening, or limitations by metabolism inhibition in biochemical reactions, among others.
After prolonged periods of low photosynthesis, starch turnover mechanism (STITT; ZEEMAN, 2012) could operate to maintain the growth and respiration in J. curcas L. plants under 150 mM of NaCl treatment.
The net photosynthesis in J. curcas L. plants subjected to 75 mM NaCl ( Figure 4B) did not show significant difference relative to control for any of the evaluated times. This indicates that 75 mM NaCl concentration could be considered a moderate salinity condition for this plant. J. curcas L. may be an appropriate species for the semi-arid regions where the availability of water in the soil and in the air is low.
The inhibition of photosynthesis during salinity could be related to a reduction in CO 2 exchange rate. It can affect the diffusion of CO 2 to the chloroplast by changes in mesophyll structure, which decreases the conductance to CO 2 diffusion within the leaf, as suggested by Delfine et al. (1999). CO 2 exchange depends on the stomatal opening degree and intracellular resistance in moving it from the intercellular air spaces to the carboxylation sites. Decrease in photosynthetic process due to the intracellular resistance depends on the species but, according to Mederski, Chen and Curry (1975), it is little or no affected when the RWC is within the range of 60%-70% in soybean plant. Thus, the decrease in photosynthetic process obtained in this work seems to be more related to stomatal than to nonstomatal regulation.
On the other hand, many enzymes involved in carbon metabolism are susceptible to salt stress. Miteva, Zhelev and Popova (1992) suggested that, in barley plants, NaCl salinity inhibited the synthesis of total soluble protein with a more pronounced synthesis inhibition of Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, EC 4.1.1.39), the key enzyme in the photosynthetic carbon reduction. The level and the carboxylating capacity of RuBisCO decreases in plants subjected to salt stress, reducing the photosynthetic capacity regardless of stomatal closure in Phaseolus vulgaris (SEEMANN;SHARKEY, 1986). The reduction in the amount of RuBisCO protein under salt stress might imply modification at different levels from transcription to post-translation level. Salt stress led to several photosynthesis-related (ATP synthesis coupled to proton transport, light reaction and xanthophyll cycle) gene down-regulation (CHAVES; FLEXAS; PINHEIRO, 2009). According to Lawlor (2002), one of the most important factors which regulate photosynthesis under salt stress is the inhibition of ATP synthesis, which limits the synthesis of ribulose bisphosphate.
Plants under various stress conditions may be affected in their photochemical reactions. (Table 2) shows the results obtained by studying some chlorophyll fluorescence components of J. curcas L. under salt stress. qN: non-photochemical quenching; ETR: electron transport rate (µmol electrons m -2 s -1 ). Different letters in each column denote significant difference between samples by Tukey test; P < 0.05; n=5; ns: non-significant difference.
The quantum yield of PSII, as indicated by Fv/Fm, was not affected by any of the salt treatments applied. The Fv'/ Fm' values, indicators of the proportion of the absorbed energy that is used in photochemical reactions and qP values, which are related to the proportion of reaction centers that are open under lighting conditions, were not significantly different between treatments for the tree times analyzed. However, qP showed differences related to time of exposure to stress conditions. Their values decreased by 31% in 150 mM NaCl treatment after 240 h compared to 6 h of treatment. It demonstrates a greater sensitivity of qP compared to Fv'/Fm'. Therefore, this damage was only important for high levels of salt in the medium during longterm stress. The non-photochemical quenching has only significant changes after 24 h between the treatment of 150 mM of NaCl and control. Although the differences obtained in ETR between control and salt stress treatments occurred only after 24 hours of stress, the tendency of this parameter was to decrease with time under saline conditions.
The capacity of photochemical reactions was evaluated through different indicators related to chlorophyll fluorescence ( Table 2).
The Fv/Fm value, indicator of the maximum potential capacity of the efficiency of PS II in darkadapted leaves, was not affected by any salt treatment applied. This indicator does not appear to be affected in some plant species subjected to various kinds of stress according to Delfine et al. (1999). Therefore, Roháček, Soukupová and Barták (2008) suggested that the Fv/Fm ratio should never be the only chlorophyll fluorescence parameter taken as an indicator of the stress status-impact to the plant tissue. Other parameters, related to the steady-state photosynthesis, are necessary to describe the status of the photosynthetic apparatus.
The lower CO 2 fixation could provoke an accumulation of ATP, NADPH and excited chlorophyll as the light reactions continue. Among the routes for energy dissipation by nonphotochemical quenching that involves the transfer of absorbed light energy away from electron transport are the heat production and the xanthophyll cycle. The salt stress conditions of our experiment seems to have activated the energy dissipation mechanisms through the qN (Table 2). After 24 h of higher salinity conditions, the xanthophyll cycle could be functioning in J. curcas L. plants.
Our results show that after 24 h of salt stress, J. curcas L. leaves decreased the electron transport rate (ETR). The lower flow of energy and its adequate consumption together with an increase of energy dissipation system were operating to prevent further damage through the qN. A better condition was maintained after 240 h of stress, with no difference in ETR values between control and both salt stress treatments. It is a presumed recuperation of the photochemistry under this condition.
Additionally, the ETR/qN ratio, taken as an estimate of the use of electrons in other processes not related to the photosynthetic CO 2 assimilation, was similar in plants under control, 75 and 150 mM NaCl at all times of stress. This suggests a balance between the photochemical and biochemistry phases, indicating that salinity did not represent a limitation of light reactions. Therefore, we suggest that leaf photochemistry in J. curcas L. is rather resistant to salt stress.
As noted in this study, the contribution of respiratory changes under salt stress seems to be a side effect compared to photosynthetic contribution. Changes in respiratory rates occur in a relatively narrow range compared to the changes in photosynthesis, which reduces the net carbon balance in the plant.
In this study, it was observed that the stomatal density is about five times higher in the abaxial (57±13 cells per mm 2 ) than in the adaxial (11±2 cells per mm 2 ) surface, and the epidermal cells quantity is 1.5 times higher in the abaxial (822±12 cells per mm 2 ) than in the adaxial (534±49 cells per mm 2 ) surface. Additionally, the stomata in the adaxial region of J. curcas L. leaves are concentrated in areas close to the leaf veins, while in the abaxial region, they are spread over the entire leaf surface (data not shown).
The number of leaf epidermal cells in J. curcas L. leaves did not change due to the saline conditions in the abaxial nor in the adaxial surface (Table 3). The stomatal density did not change in the adaxial surface but rather in the abaxial surface, in which it increased after 24 and 240 h in 75 and 150 mM NaCl treatments (Table 3)

1
In the abaxial leaf surface it was observed that the 150 mM NaCl treatment promoted the closure of the stomata at the beginning (6 h) ( Figure 5). After 24 hours and 240 hours of exposure, no significant differences were observed between saline treatments and the control group. Reduction in the size of the stomata increases stomatal resistance, which limits the excessive water loss by transpiration. J. curcas L. plant controls the loss of water through the stomata by closing them during the first 6 hours of stress, but with the longterm salt stress the control is obtained with the modification of stomatal density. The much higher stomatal density indicates that salt stress induces the formation of leaves with an altered anatomy. More stomata in the abaxial surface of the leaf permit a tighter regulation of water loss. In this way, the J. curcas L. plant can maintain the water supply to the cells and the RWC even after 240 h of salt stress.
According to our results, the reduction in photosynthetic rate could not be due to stomatal limitation because there was no stomatal closure in response to salinity ( Figure 5). The reduction in photosynthetic rate could be related to other factors as mentioned previously.

Effects of Salinity Stress on SOS and HKT1 Genes
Information related to salt tolerance of J. curcas L. at molecular level is insufficient. We analyzed, in J. curcas L. submitted to saline conditions for 6 h, the gene expression pattern of key genes involved in salinity tolerance ( Figure 6). SOS1 expression in root was repressed under 75 and 150 mM of NaCl while in leaf its expression was not modified. Amounts of mRNA increased for HKT1 gene in root and leaf of plants at 75 mM of NaCl, but decreased at 150 mM of NaCl in both organs compared to control. Gene expression profile for SOS3 gene was not modified in leaf and root as well as SOS2 in leaf. Figure 6. Semi quantitative RT-PCR analysis showing differential gene expression depending on the salt level for HKT1 gene of Jatropha curcas L. The expression of each gene was compared relative to its expression in control gene (ACT). DNA samplings were carried out at 24 and 240 h after treatments with 0, 75 and 150 mM NaCl.
According to Zhang et al. (2014), 1504 and 1115 genes modified their expression in root and leaves, respectively, in J. curcas L. under 100 mM of NaCl treatment, but none of them belong to the SOSs and HKT1 genes. In our work we found that mainly SOS1 and HKT1 modified the expression pattern depending the NaCl concentration. Up and down regulation of HKT1 at 75 mM and 150 mM respectively indicate the tolerance of the J. curcas L. plant to salt stress. At 75 mM of NaCl, the Na + could enter into the cell, as was detected by Díaz-López et al. (2012) using 60 mM of NaCl, and the plant has mechanisms to deal with it. At higher Na + concentrations in the medium (90-150 mM), there was no increase in the Na + concentration in the root, indicating some efficient mechanism for salt exclusion, as suggested by Díaz-López et al. (2012). Our result supports the idea that the plant activates the mechanism to avoid the entry of Na + , inhibiting the HKT1 transport, detected in our work as less HKT1 transcript, and activating the Na + exclusion, detected in our work as higher SOS1 transcript at 150 mM than at 75 mM of NaCl in root.

CONCLUSIONS
Photosynthesis activity of J. curcas L. was not affected by moderate salinity levels of 75 mM NaCl. Additionally, other physiological and morphological traits such as stomatal opening and membrane damage in leaves were not affected by 75 mM NaCl nor even by the highest salt level applied (150 mM NaCl). As a consequence, leaf growth (RLGR and ALGR) was not affected by a salinity of 75 mM NaCl, equivalent to approximately 7 dS m -1 . Modified expressions of SOS1 and HKT1 genes, different for 75 and 150 mM of NaCl, indicates that the plant is able to tolerate Na + in the tissue and has efficient mechanism to avoid damage. These results suggest that J. curcas L. is an appropriate species to be cultivated in semi-arid regions.