First case report of cryptococcosis due to Cryptococcus decagattii in a pediatric patient in Argentina

We report the first case of cryptococcosis due to Cryptococcus decagattii in an immunocompetent pediatric patient from an indigenous community in Argentina with a successful outcome. Two isolates (blood, cerebrospinal fluid) were genotyped by restriction fragment length polymorphism of the orotidine monophosphate pyrophosphorylase (URA5) gene as VGIV and identified by multi-locus sequence typing as C. decagattii. Matrix-assisted laser desorption/ionization time of flight mass spectrometry identification indicated genotype VGIII. The minimum inhibitory concentration of amphotericin B, fluconazole, itraconazole, and voriconazole was determined (cerebrospinal fluid: 0.25, 16, 0.12, and 0.12, blood: 0.25, 4, 0.12, and 0.06, respectively, all in mg/L).


CASE REPORT
A seven-year-old female of the indigenous community Wichi (Salta Province, Argentina) was admitted to a local medical institution on July 17, 2017 with postprandial vomiting, abdominal pain, and constipation. On July 19, 2017 (day 0) she began to show clinical signs of meningoencephalitis and was referred to a public hospital. The girl presented with a depressed level of consciousness, lethargy, psychomotor excitation, stiff neck, a cardiac frequency of 115 bpm, a respiratory frequency of 16 bpm, and a Glasgow scale of 10/15 (motor response: 4, eye response: 3, verbal response: 3). Ophthalmological assessment revealed pupils with slight anisocoria, strabismus and left-sided nystagmus, and left unilateral papilledema grade II. Signs of cerebral edema were observed in a computed tomography scan.
Blood (BS) and cerebrospinal fluid (CSF) samples were obtained and empirical antimicrobial treatment with ceftriaxone (1 g/12 h), amphotericin B lipid complex (2.5 mg/kg/d), and acyclovir (60 mg/kg/d) was initiated on day 0. Her body weight was 21.5 kg and her blood glucose level 90 mg/dL.     The abdominal ultrasound and chest radiograph were normal, as were blood cell counts and liver and kidney function tests. Electrolyte levels in the blood were also relatively normal with a slight hyponatremia and elevated C-reactive protein (23.1 mg/dL). The erythrocyte sedimentation rate was determined as 7 mm/h, white blood cell (WBC) count as 7,300 cells/μL (68 % neutrophils, 21 % lymphocytes), the hematocrit as 37 %, and hemoglobin as 121 g/L. Microscopic observation of the CSF specimen after staining with India ink showed the presence of rounded encapsulated yeasts consistent with Cryptococcus. Isolates obtained from the initial CSF and BS cultures were sent to the National Reference Laboratory for further studies. They showed mucoid colonies in yeast medium agar plates and rounded budding yeasts in 5% malt extract, growing well at 37ºC. Urease, phenoloxidase, and canavanine-glycinebromothymol blue (CGB) tests were positive. Identification by MALDI-TOF MS was performed as described previously 4 . This technique identified both isolates as C. gattii s.l. Best match results were obtained with the main spectra (MSP) of genotype VGIII strains. Molecular characterization by RFLP of the URA5 gene was performed as described previously 2 and both isolates showed a genotype VGIV profile. MLST was performed according to the International Society for Human and Animal Mycology (ISHAM) consensus scheme 5 . MLST clustering analysis showed that our isolates represent a novel sequence type related to the genotype VGIII that clustered with the strains WM1802 (ST100) and WM 1804 (ST101) from Mexico (Figure 1). These strains are related to the previously named "VGIIIc" strain 7685027 from Southern California 6 and to the AFLP10 strain CBS11687 isolated in Spain from a Mexican patient that is the type strain of C. decagattii 3 . The alleles and sequence types were: ST=545, CAP59=95, GPD1=61, IGS1=110, LAC1=41, PLB1=45, SOD1=131, and URA5=57. All alleles except for LAC1 represent a new allele type and result in a new sequence type entry in the MLST database. The URA5 sequence showed a single nucleotide polymorphism (SNP) at position 528, which is the restriction site of the SAU96I enzyme, leading to a VGIV genotype profile.
Antifungal susceptibility tests were carried out by determining the minimum inhibitory concentration (MIC) according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) Definitive Document (E. Def) 7.3.1 reference method 7 . Isolates were tested against amphotericin B, fluconazole, itraconazole, and voriconazole (Merck, Buenos Aires, Argentina). The MIC values for the CSF isolate were 0.25 mg/L amphotericin B, 16 mg/L fluconazole, 0.12 mg/L each itraconazole and voriconazole, and for the BS isolate were 0.25 mg/L amphotericin B, 4 mg/L fluconazole, 0.12 mg/L itraconazole, and 0.06 mg/L voriconazole. Current methodologies to determine the MIC do not include clinical breakpoints for C. gattii s.l., and hence no categorical interpretation of results was possible. We used the epidemiological cutoff value (ECV) proposed by Espinel Ingroff et al. to interpret our results [8][9] . Both isolates were categorized as wild-type (WT) for all antifungals tested, except for non-WT categorization of the CSF isolate for fluconazole (MIC=16 mg/L).

DISCUSSION
Previous studies have reported the presence of C. gattii genotypes VGI, VGII, and VGIII in clinical and environmental sources in Argentina and other South American countries, but genotype VGIV has only been isolated in Colombia 10-11 . C. decagatti has been rarely isolated and with only 5 reports worldwide 3 . We present here the first case report of cryptococcosis caused by C. decagattii in Argentina and also in South America in a patient belonging to the indigenous community Wichi whose people live in huts made of branches in close physical contact with nature and domestic animals. This extensive exposure to nature may be a potential source of infection 1 . On the other hand, the patient presented with signs of malnutrition that may also have been a predisposing factor.
Meningoencephalitis caused by C. gattii s.l. usually requires aggressive management of the elevated intracranial pressure and extensive antifungal treatment 1 . In the present case, several therapeutic CSF drains were required to alleviate the intracranial pressure and the antifungal treatment regimen included administration of amphotericin B and fluconazole twice for an extensive period of time. Rapid differentiation between C. gattii s.l. and C. neoformans s.l. was possible with the CGB test and MALDI-TOF MS. This differentiation is important because these complexes differ in their clinical presentation and management 1,3 . Molecular characterization of C. gattii species is important for ecological and epidemiological purposes. In the present case, RFLP of the URA5 gene misidentified the isolates as genotype VGIV, but the URA5 sequence contained the same SNP described previously for two isolates of the VGIII serotype C from Mexico 12 . MALDI-TOF MS identification of the isolates showed the best match results with the MSP of the genotype VGIII strains. This is in agreement with previous studies demonstrating a high potential of this technique to correctly differentiate between the major genotypes 4 .