Impact of feeding cottonseed coproducts on reproductive system of male sheep

Cottonseed coproducts contain gossypol which can have specific effects upon the male reproductive system. We evaluated the reproductive development of male lambs close to puberty fed on three cotton coproducts. Twenty-four 5-month old male lambs received four diets: 20 % of dry matter intake of whole cottonseed (WCS), high oil cottonseed meal (CSC), cottonseed meal (CSM), and a control group (CTL) without any cottonseed coproducts. Free gossypol intake was 16.32, 6.98, 5.47 and 0 mg kg−1 BW−1 d−1 for WCS, CSC, CSM and CTL, respectively. Every 15 days, the animals were weighted and serum and semen samples were collected. After 95 days, testis samples were collected for analysis under light and transmission electron microscopes. The CTL group had higher testosterone concentrations than CSC at the end of the trial and lower total sperm defects, higher mass movement and higher scores for seminiferous epithelium than other treatments. The WCS and CSC groups showed higher levels of segmental aplasia lesion in sperm than other diets, which showed that high levels of gossypol led to higher occurrence of this lesion. Cottonseed coproducts had a negative impact on the reproductive system of pubertal lambs regardless of gossypol concentration. Therefore, the use of cottonseed coproducts to feed lambs earmarked for reproduction is not safe.


Introduction
Brazilian cottonseed production in 2014 was 1,672.300t and it is expected that production will grow at 3 % yr −1 until 2024 (MAPA, 2014), which indicates abundant availability of cottonseed coproducts.This stands out as an alternative source of protein and energy for animal diets, which can reduce cost.
However, a major limitation for using these coproducts in animal nutrition is high levels of gossypol.Studies on humans (Coutinho, 2002), monkeys (Shandilya et al., 1982) and rats (Chang et al., 1980;Oko andHrudka, 1982, 1984b) have indicated that gossypol can have particular effects upon the male reproductive system, resulting in impaired spermatogenesis and reduced sperm motility.The gossypol effect on the endocrine system is not clear and few studies have evaluated it (De Andrade et al., 2006).Furthermore, gossypol is considered a promising drug for male contraception in humans (Coutinho, 2002).
The toxicity level of gossypol is considered low in ruminants, because the rumen environment promotes binding of free gossypol to proteins, which renders it physiologically inactive (Reiser and Fu, 1962).However, studies with ruminants have shown a wide variety of results, ranging from minimal (Chase et al., 1994) to severe pathological effects (Randel et al., 1992;Chenoweth et al., 1994).For example, whole cottonseed fed to deer was considered an efficient male contraceptive due to low semen quality during treatment and full recovery the following year (Gizejewski et al., 2008).
Certain studies using cotton coproducts failed to demonstrate a relationship between higher gossypol concentration and more severe seminal pathologies.This may indicate that the availability of free gossypol may differ between feedstuffs (Chase et al., 1994).The majority of gossypol toxicity and cottonseed coproduct feeding trials used postpubertal males.Few studies, mainly using bulls and rats (Chase et al., 1994;De Andrade et al., 2006) and none with sheep, have evaluated the effect of feeding gossypol to prepubertal males.De Andrade et al. (2006) argued that the pubertal phase is especially vulnerable and gossypol may provoke more damage.On the other hand, Chase et al. (1994) showed that gossypol did not affect semen quality at puberty, which demonstrates that the effect of feeding gossypol to prepubertal males on subsequent reproductive development is not clear.Therefore, we evaluated the effect of feeding three cotton coproducts with different gossypol concentrations on reproductive development in lambs close to puberty.

Animals, feeds and experimental design
Twenty-four Santa Inês male lambs with mean body weight (standard error) equal to 20.6 (± 1.9) kg and initial age of 5 months were housed in individual covered pens with a concrete floor.Animals were divided equally into four groups and fed the following diets (Table 1): whole cottonseed (WCS); pressure-extracted cottonseed meal with high oil (CSC); solvent-extracted cottonseed meal with low oil (CSM) and control (without cottonseed co-products, CTL).The study was carried out in Brasília, Federal District, Brazil (15°47' S; 47°56' WGr, altitute of 1172 m).The University Animal Ethics Committee (protocol 33/2009) approved this experiment.The experimental period lasted 95 days and was preceded by an adaptation period of 14 days.The diets were elaborated according to NRC (2007) for a daily body weight gain of 200 g d −1 (Table 1).The ratio concentrate:forage was 50:50 in dry matter basis and Coast cross (Cynodon dactylon (L.) Pers) hay was used as forage.Mineral salt and urea were added to concentrate in the same amount for all groups.According to bromatological analysis of each diet ingredient, soybean oil was added to the concentrate in CSC, CSM and CTL, aiming to equalize the ether extract.The determination of free gossypol concentrations in the diet were achieved through the UV-VIS spectrophotometry method adapted from Wang (1987).

during peripubertal period
The animals were fed twice daily, morning (8h00) and afternoon (17h00).The amount of feed offered was adjusted according to animal consumption, to achieve leftovers equal to 10 % of the feed offered.Animals were weighed fortnightly after 10 hours fasting.

Hormone assays
Fortnightly at 9h00, blood samples were collected to determine cortisol and free testosterone concentrations employing radioimmunoassay commercial kits (DPC Medlab Coat-A-Count).The raw data in cpm (counts per minute) obtained by gamma counter were analyzed by the logit-log plot develop by Rodbard and Lewald (1970) cited by Geiger (1992).All samples and standards were measured in duplicates.The ranges of calibration curves were 0.63 to 52 pg mL −1 for free testosterone and 1 to 46 µg 100 mL −1 for cortisol.The average intra-assay coefficient of variation was 10 % for testosterone and 5 % for cortisol, and inter-assay coefficients were 18 % for testosterone and 5 % for cortisol.

Seminal parameters
The scrotal size (circumference, length and width) was measured fortnightly.At the same time, semen samples were collected through eletroejaculation and seminal parameters evaluated according to the Manual for Andrological Examination and Semen Evaluation (BCAR − Brazilian College of Animal Reproduction).The volume, mass movement (0 to 5) and semen aspect were determined by observation in the collector tube.Then, under light microscopy, fresh sperm drop was evaluated for progressive motility (%) and vigor (0 to 5).Semen samples were fixed in buffered formal-saline (1 %) to evaluate sperm morphology and concentration under a contrast phase microscope (wet solution, 1000x).Sperm concentration was performed using a Neubauer chamber, adjusting the result to the dilution used.Morphology was evaluated by looking at 200 spermatozoa per sample to determine the proportion of abnormal spermatozoa: head shape defects (underdeveloped, irregular contour, neck, small and large heads), acrosome defects (reacted, vesicular, detached and bent), midpiece (cracked, gap, bent, thick, notch, broken, abaxial, irregular and pseudodroplet), and tail defects (simple and strongly bent, coiled, proximal and distal droplet as well as coiled around head) (Larsen and Chenoweth, 1990;Chenoweth et al., 1994).

Light microscopy analyses
At the end of the experimental period, the animals were slaughtered and samples of testis were collected.Four samples from each testis were stored in formaldehyde solution (10 %), then embedded in paraffin, cut at a thickness of 3 µm and stained with hematoxylin and eosin.Digital capture of images was performed under microscope coupled with a colored digital CCD camera.A total of five images were taken per animal and, from each image, five randomly chosen seminiferous tubules were analyzed, totaling 25 tubules analyzed per animal (McManus et al., 2010).The diameter of tubule and lumen were measured at two points of each seminiferous tubule, using the MICAM ® software (version 1.4).The seminiferous epithelium thickness was obtained by the difference between the lumen and tubule diameters.

Transmission electron microscopy analyses
Two testis samples (1 mm thick) from each animal were fixed in Karnovisky's fixative (2.5 % glutaraldehyde, 2 % paraformaldehyde, 0.1 M phosphate buffer, pH 7.2) and stored at 4 °C.Samples were rinsed in cacodylate buffer, cut into smaller pieces and post-treated in 1 % osmium tetroxide for 1 h, and uranyl acetate overnight at 4 °C.Tissue blocks were then dehydrated by exposure to graded concentrations of acetone (30, 50, 70, 90 and 100 %) and embedded in low viscosity resin.The ultrathin sections (80 nm) were placed on uncoated copper grids (200 mesh), counterstained with uranyl acetate and lead citrate.The sections were examined under a transmission electron microscope at 80 kV.Image analysis was carried out whereby three scores for each grid were given, without previous knowledge of animal identification to avoid bias.Sertoli cells were scored (1 to 10) according to shape, size, vacuolization degree, mitochondria and other abnormalities.Spermatogonial cell lineage (spermatogonia, spermatocytes and spermatids) received a score (1 to 10) which considered the presence of layers representing the seminiferous cycle stages and the integrity of cells (Wrobel et al., 1995;Steger and Wrobel, 1996).Lower scores meant a high number of defects.The spermatozoa in tubule lumen were evaluated for presence or absence (1 or 0) of segmental aplasia of the mitochondrial sheath (often abbreviated to SAMT) which is considered a gossypol-induced sperm abnormality (Oko and Hrudka, 1982;Chenoweth et al., 2000).
Semen from the last collection in the experimental period was also collected for evaluation by transmission electron microscopy (TEM).The preparation procedure was similar to that used for the testis samples, except that a prior step was carried out where semen was embedded in a polymer.Spermatozoa were given a score (1 to 10) according to the number of abnormalities found (named forward as SIS).The highest score (10) meant that no sperm defects had been found.The mitochondrial sheaths were analyzed to identify the segmental aplasia (named forward as SAMS), so if this lesion was identified the grid received "1" and, if not, "0".

Statistical analyses
The average daily weight gain (ADG) was obtained by linear regression of weight per day of experiment for each animal and angular coefficient (b) was used in the analysis of variance using the MIXED procedure from SAS ® (Statistical Analysis System, version 9.3).ADG was submitted to a regression analysis considering the gossypol level, testing linear and quadratic effects.
The hormone results were analyzed as repeated measures using the MIXED procedure.Treatment (t), days of experiment (d) and interaction between them (t*d) were used as fixed effects and animal as a random variable.
Vigor and mass movement were analyzed by the Kruskal-Wallis test using the NPAR1WAY procedure of SAS ® .The sperm pathologies were analyzed by MIXED procedure fitting as fixed effects: t, d and t*d; and animal (inside treatment) as random effect.Sperm volume, motility, concentration and scrotal size (circumference, length and width) were analyzed as repeated measures using the MIXED procedure.Regression analysis between these traits and the gossypol level in diet (linear and quadratic effect) was carried out.Finally, a factor analysis was used to verify the relationship between these variables.
Analysis of variance for seminiferous tubules measures was carried out using treatment, photo, tubule inside photo, and final weight of animal as fixed effects.Correlations, regression with the gossypol level in diet, and factor analysis were carried out.The scores obtained in testis and semen samples observed in TEM were evaluated using a logistic regression testing effect of treatment, using contrast between each pair of treatments, and also effect of the gossypol level in diet (linear, quadratic and cubic regression).Correlation and factor analysis also were applied to this data.
Normality of residuals and homogeneity of variance tests were performed on raw data.Transformations were carried out where necessary.The results of sperm pathologies and motility (percentages) were transformed using the arc sine of the square root.In all mixed model analyses, the covariance structure that best adjusted to data was chosen according to the lowest Akaike Information Corrected Criterion (AICC) and Bayesian Information Criterion (BIC).Means were compared by least square means when p < 0.05, using Tukey adjustment with PDIFF statement in SAS ® .

Results
The estimated free gossypol (FG) intake per day was 16.32, 6.98, 5.47 and 0 mg kg of Body Weight −1 (BW) for WCS, CSC, CSM and CTL, respectively.Average daily weight gain (ADG) was 138 ± 8.0 g d −1 , which did not differ between treatments (p > 0.05).Average dry matter intake (DMI) was 906 ± 14, 883 ± 14, 895 ± 14 and 843 ± 14 g d −1 for WCS, CSC, CSM and CTL, respectively.The DMI for CTL was lower (p < 0.05) than for other groups.Free testosterone concentrations increased with time as expected, because the animals were in the pubertal period.At 90 days into the experiment, the CTL group had higher testosterone concentration than the CSC group (Figure 1A).Cortisol concentrations decreased with days into the experiment and there was no treatment effect (Figure 1B).
The volume ejaculated, sperm concentration and motility increased as animals grew, but was not affect-ed by treatments (Figure 2).The scrotal size measures (circumference, length and wide) also increased according to time without treatment effect.The regressions of these traits with the gossypol level in the diet were not significant.The factor analysis also showed that the gossypol level was not related to these traits.
Sperm vigor was not affected by treatments.However, the animals from the CTL group showed higher scores for mass movement than the others.Animals   from CSM and CSC groups had more head defects and WCS and CSM groups had higher number of coiled tail compared to CTL group.CTL group had the lowest total sperm defects (Table 2).Days of experiment and interaction (t*d) did not have significant effect on sperm pathologies.The factor analysis showed that the gossypol level in the diet was not related to any sperm pathologies (Figure 3).The seminiferous tubule measures showed a significant treatment effect.The CSM group had the thickest seminiferous epithelium (Table 3).The regression and factor analyses showed that the gossypol level in the diet did not affect it.The factor analysis demonstrated that thicker seminiferous epithelium determine higher sperm concentrations in the ejaculated semen.There was a significant and positive correlation between sperm concentration and seminiferous tubule measures (0.27 < r < 0.44).
In relation to spermatozoa observed in TEM, the WCS and CSC group had lower scores for SIS than the CTL and CSM groups (odds ratios equal to 3.9 between WCS and CSC vs CTL, and odds ratios equal to 18.6 between WCS and CSC vs CSM).The logistic regression analyzing the SAMS (Figures 4A, B, C and  D) showed that the CSM group differentiated from the WCS group (odds ratio = 14, modeling for non lesion probability, and p = 0.04 in contrast test).The contrast between CTL and CSM vs CSC and WCS also was significant.The contrast test analyzing effects of the gossypol level in the diet showed a linear trend Different letters in the same row mean significant statistical differences (p < 0.05).SD: standard deviation.(p = 0.08).Sixty-seven percent (67 %) of sperm evaluated from the WCS group had SAMS which were 13 % in the CSM group (Figure 4D).
Treatments and the gossypol level in diets did not influence the Sertoli cells score.The scores for seminiferous epithelium were higher in the CTL group compared to others (odds ratios = 2.96 to WCS vs CTL, 2.49 to CSC vs CTL and 2.20 to CSM vs CTL).The main alterations observed in spermatogonial lineage cells were vacuolization inter and intra cellular and lower cell size with decrease in cytoplasmic ground substance, which is similar to that found by Hoffer (1983).The SAMT were not affect by treatments.
The scores for SIS and SAMS showed significant correlations with the seminiferous epithelium thickness (0.62; -0.49, respectively).The factor analysis (Figure 5) showed a group of variables formed by seminiferous tubule measures, sperm concentration, Sertoli and SIS scores, opposing SAMS.This demonstrated that the thicker epithelium led to higher scores for SIS and lower levels of mitochondrial sheath aplasia.

Discussion
To the best of our knowledge, this is the first study to evaluate the effect of feeding cottonseed co-products during the pubertal phase of male lambs.This study shows that cottonseed co-products can impair reproductive development in male lambs, regardless of gossypol concentration in diet.Feeding up to 16.32 mg kg BW −1 d −1 of free gossypol did not impair lamb growth.Chase et al. (1994) showed that bulls fed whole cottonseed (60 mg kg BW −1 d −1 of free gossypol) from weaning through puberty had lower body weight gain and reached puberty at an older age than bulls fed cottonseed meal (6 mg kg BW −1 d −1 ) or soybean meal.These authors argue that, as puberty was reached at similar body weight and scrotal circumferences among treatments, delayed puberty in bulls fed whole cottonseed may have been due to a lower energy balance and it was not related to gossypol.Therefore, the main reason for the difference to the present study may be related to diet constitution.Our diets had an ether extract (EE) maximum equal to 8 %, while these other authors utilized close to 10 % of EE in a whole cottonseed diet, which may impair fiber digestibility and decrease forage consumption (NRC, 2007).
The cottonseed diets did not affect scrotal size, sperm volume, motility, vigor and concentration in the present study, and these measures increased over time.In similar studies (Chase et al., 1994;Chenoweth et al., 1994), gossypol also did not affect scrotal size, sperm quantity or quality.Cunha et al. (2012) found that progressive motility and vigor of sperm cells were influenced by whole cottonseed, with a downward linear trend as the gossypol level in diets increased (6.8, 9.2 and 11.5 mg kg BW −1 d −1 of free gossypol).Dabrowski et al. (2000), studying gossypol toxicity in fish, stated that the antifertility effect of gossypol is related to how efficiently gossypol crosses a general circulation-gonadal barrier.Therefore, different permeability of reproductive organs due to species, age and metabolic rate may explain different results of experiments using a similar gossypol dose.
The CSM group had greater seminiferous tubule diameter, low lumen and, consequently, thicker seminiferous epithelium than others.The gossypol concentration in diets did not influence the seminiferous tubule measures observed under light microscope.In a similar study (Chase et al., 1994), bulls fed cottonseed meal (6 mg kg BW −1 d −1 of free gossypol) had greater luminal diameters, thinner epithelial walls, and fewer germ cell layers than bulls fed whole cottonseed (60 mg kg BW −1 d −1 of free gossypol), which was unexpected due to higher free gossypol concentration in whole cottonseed than meal.In gossypol treated rats, sperm morphology was severely compromised, but the epithelium in testis appeared morphologically normal (Hoffer et al., 1987;De Andrade et al., 2006).Some authors argue that gossypol targets the epididymis, disturbing both the structure and function of this organ, and presumably disrupts sperm maturation without alterations in testis (De Andrade et al., 2006).Therefore, there are controversial results for the impact of gossypol on seminiferous tubule measures.Moreover, the impact of these measures on sperm production and quality are not clear.In the present study, sperm concentration was higher with thicker seminiferous epithelium as shown by factor analysis.
The animals from the CTL group had a higher free testosterone concentration at 90 days than the CSC group.The gossypol level in diets was not related to free testosterone concentration because some animals fed diets with a higher level of gossypol also had a higher level of free testosterone (e.g.WCS opposite CSC).In a similar study, Chase et al. (1994) did not observe differences in testosterone concentrations between pubertal bulls fed diets containing gossypol and those fed soybean meal.
The effect of gossypol on the endocrine system is controversial.Several studies in monkeys and rats showed that gossypol reduced fertility without changes in testosterone, or other androgens, or luteinizing hormone (Shandilya et al., 1982;Wang et al., 1984;Soufir et al., 1989).However, a number of studies in rats and deer indicated that gossypol has an inhibitory effect on testosterone production by the Leydig cell via a subsequent lesion in pregnenolone formation (Oko and Hrudka, 1984a;Gizejewski et al., 2008).In these studies, the antifertility effect of gossypol appears secondary to the decrease in testosterone synthesis (Oko and Hrudka, 1984a;De Peyster and Srebnik, 1988;Dabrowski et al., 2000;El-Sharaky et al., 2010).Udoh et al. (1992) reported that Sertoli and Leydig cells showed progressive regression due to gossypol administration.El-Sharaky et al. (2010) showed significant increases in the activities of testicular 17b-HSD and 17-ketostroid reductase in gossypol treated groups compared to the control group, which may lead to increased degradation of testosterone which may explain the reduction of serum testosterone concentrations.Moreover, it has been shown that gossypol interferes with key steroidogenic enzymes such as 5α-reductase and 3α-hydroxysteroid dehydrogenase in rat testis (Moh et al., 1993).Randel et al. (1992), in a comprehensive review, affirmed that the effects of gossypol on testosterone concentrations are not consistent (i.e., no effect or decreased).According to De Andrade et al. (2006), the great contradiction in the endocrinal effects of gossypol may be due to different animal species, different doses and times of treatment, or due to different administration routes.
The cortisol concentration did not differentiate between treatments and was also not related to testosterone.The hormone cortisol is the end product of the activation of the hypothalamus-pituitary-adrenal (HPA) axis and the hormone testosterone is secreted by activation of the hypothalamus-pituitary-gonadal (HPG) axis (van der Meij et al., 2012).Zilioli and Watson (2012) commented on a significant interaction between the HPG and HPA axes status, so a high cortisol level can inhibit the release of testosterone.In this study, cortisol was used as an indicator of stress condition and to identify possibly high-cortisol animals, which could affect testosterone concentrations.Gossypol did not affect the cortisol concentrations, so the interaction between the HPG and HPA axes did not affect the results of the study.
Sperm pathologies showed that animals from the CTL group had a lower total of spermatozoa defects.The Sci. Agric.v.73, n.6, p.489-497, November/December 2016 main sperm defects found in animals that receive cotton coproducts compared to the control group were isolated (detached) heads, coiled tail and proximal cytoplasmic droplet.In another study (Cunha et al., 2012) using crescent concentrations of whole cottonseed fed to sheep (6.8, 9.2 and 11.5 mg kg BW −1 d −1 of free gossypol), the percentage of total defects increased linearly: for each 1 % increase of whole cottonseed in the diet, there was an increase of 0.2 % in total defects.In the morphological analyses, a greater occurrence of defects such as broken acrosome, folded tail, and strongly folded tail were seen (Cunha et al., 2012).Chenoweth et al. (1994), studying bulls fed with whole cottonseed (16.4 mg kg BW −1 d −1 of free gossypol), observed an increased proportion of sperm midpiece abnormalities, which stabilized at 52 to 62 %.In rats that received gossypol daily (15 mg kg BW −1 d −1 ) from weaning through puberty, there was a significant increase in sperm with abnormal morphology in the vas deferens of treated animals, with the most frequent abnormality being isolated sperm heads (De Andrade et al., 2006).
The sperm pathology and ultrastructural results of this study agree with Oko and Hrudka (1982) who stated that there is a common pathogenic path linking many of the major sperm midpiece abnormalities with known spermatoxic effects of gossypol on the structural organization of the sperm midpiece in late spermatogenesis.Gossypol appears to cause damage to the mitochondrial sheath of cells during the latter phases of spermatogenesis, with lesions being first detected in elongating spermatids.Therefore, the types of lesion first observed at extragonadal sites suggested structural failure in already weakened structures, possibly exacerbated by the onset of sperm motility.
In ultrastructural studies, segmental aplasia of the mitochondrial sheath has been consistently identified with gossypol treatment in rats, monkeys, rabbits and bulls (Oko andHrudka, 1982, 1984b;Chenoweth et al., 2000).This lesion was considered to be pathognomonic for gossypol spermatoxicity (Oko and Hrudka, 1982), which allows it to be differentiated from other causes of sperm axonemal disruption.In the present study, there was a linear trend between gossypol level in diet and segmental aplasia lesion in sperm.The WCS and CSC group showed higher levels of SAMS than CSM and CTL.However, we do not agree on categorization of this lesion as pathognomonic for gossypol spermatoxicity, because we found it in spermatozoa from the CTL group (Figure 4D).Others authors have also occasionally observed this lesion in bulls that did not receive cotton products (Burgess and Chenoweth, 1975).
In summary, the CTL group had the lowest total sperm defects and better seminiferous epithelium structure.When compared to the CTL group, CSM had more isolated head defects; WCS and CSM had more coiled tail defects, and CSC had more proximal cytoplasmic droplets.Animals from the CSC group had lower testosterone concentrations.WCS and CSC had lower SIS and a higher number of SAMS.Therefore, the overall reproductive status of CTL group was better than the others.Consequently, the cotton coproducts had a nega-tive impact on it.However, the increase in gossypol concentration in the diet did not demonstrate a proportional increase in injuries.Probably, different feedstuffs (gossypol sources) led to different rumen functioning which can affect the free gossypol bioavailability, as described by Chase et al. (1994).
In ruminants, there are difficulties in controlling the amount of bioavailable gossypol.In studies where total gossypol concentrations in plasma were checked and adverse effects were found, the concentrations varied from 26.2 to 73.0 µg g −1 in treated bulls and no gossypol was detected in the plasma of control bulls (Chenoweth et al., 1994).In contrast, another study on bulls where no qualitative or quantitative semen changes were detected following the feeding of cottonseed meal for 132 days, gossypol was not detected in the plasma nor in the other tissues of treated bulls (Jimenez et al., 1989).Moreover, there was a variation between individuals.In studies on humans, 15 % of men failed to suppress spermatogenesis, although they had similar plasma gossypol levels to the others who had sperm suppression (Coutinho, 2002).

Conclusions
The cottonseed coproducts did not impair lamb growth.The cotton coproducts have a negative impact on the reproductive system of male pubertal lambs regardless of gossypol concentration (up to 16.32 mg kg BW −1 d −1 ).Therefore, we do not recommend feeding cotton coproducts to prepubertal male lambs if these animals are to be used for reproduction in the future.

Figure 1 −
Figure 1 − Free testosterone (A) and Cortisol (B) concentrations in serum from Santa Inês lambs fed with whole cottonseed (WCS), cottonseed meal with low oil (CSM), cottonseed meal with high oil (CSC) and a control without use of cottonseed (CTL).Different letters between treatments in the same experimental day means statistically significant difference between treatments (p < 0.05).

Figure 2 −
Figure 2 − Least square means of volume ejaculated, sperm motility, sperm concentration and scrotal circumference from lambs receiving: whole cottonseed (WCS), high oil cottonseed meal (CSC), low oil cottonseed meal (CSM) and control (CTL) without cottonseed co-products.There was no difference in means between treatments (p > 0.05).

Figure 3 −
Figure 3 − Factor analysis of scrotal size measures, sperm pathologies, gossypol level in diet and immediate sperm traits from Santa Inês lambs fed with whole cottonseed, cottonseed meal with low oil, cottonseed meal with high oil and a control without use of cottonseed for 95 experimental days.The values between parentheses show the amount of variance explained by each factor.CONC: sperm concentration; MOT: sperm motility; SL: scrotal length; SW: scrotal width; SC: scrotal circumference; DAYS: days into experiment; GOS: gossypol level in diets; CRACKED: cracked midpiece; IRREG: irregular midpiece; PN, TN: partially and totally notch midpiece, respectively; COILED: coiled tail; CTH: coiled tail around head; TAIL: total tail defects; DEFECTS: total number of spermatozoa with defects.

Figure 4 −
Figure 4 − Electron micrographs showing aplasia of mitochondrial sheath (arrows) in spermatazoa from lambs fed with whole cottonseed (A), cottonseed meal with high oil (B), cottonseed meal with low oil (C), and a control without use of cottonseed (D) for 95 experimental days.

Figure 5 −
Figure 5 − Factor analysis of sperm and testis traits observed in light and transmission electron microscopy from lambs fed with whole cottonseed, cottonseed meal with low oil, cottonseed meal with high oil and a control without use of cottonseed for 95 experimental days.The values between parentheses show the amount of variance explained by each factor.SIS: spermatozoa integrity in semen; SAMS: Segmental aplasia of mitochondrial sheath in spermatozoid observed in semen; Sertoli: score for Sertoli cells in testis; Epithelium: scores for seminiferous epithelium observed in transmission electron microscopy; SAMT: Segmental aplasia of mitochondrial sheath in spermatozoid observed in testis; TD: seminiferous tubule diameter; TL: seminiferous tubule lumen; SE: seminiferous epithelium thickness; SC: sperm concentration.

Table 1 −
Ingredients (g kg −1 of dry matter of concentrate), chemical composition (on dry matter basis) of each ingredient of diet and free gossypol content in the experimental concentrates.

Table 2 −
Least square means of sperm defects* (%) from Santa Inês lambs that received whole cottonseed (WCS), high oil cottonseed meal (CSC), low oil cottonseed meal (CSM) and control diet without cotton co-products (CTL).*Showedonly sperm defects (pathologies) that had differences between treatments or had means above 1 %.Different letters in the same row me statistical difference (p < 0.05).SD: standard deviation; cyt.: cytoplasmic.MP defects: total midpiece defects.

Table 3 −
Least square means of tubule diameter, lumen diameter and seminiferous epithelium thickness (µm) of lambs fed with whole cottonseed (WCS), cottonseed meal with low oil (CSM), cottonseed meal with high oil (CSC) and a control without use of cottonseed (CTL).