Characterization of fractions of aqueoussoluble constituents of the mushroom Pleurotus ostreato-roseus Sing. and its effects on the serum protein profile of mice

^IDepartamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, 52171-900, Recife, PE

^IIDepartamento de Medicina Veterinaria, Universidade Federal da Paraíba, Campus VII, Patos, PB, Brazil

ABSTRACT

Various species of mushrooms belonging to genus Pleurotus (Fr.) Kumm. are known by its nutritional value and medicinal properties, particularly anti-tumor activity and immunomodulation, related to structural polysaccharides. In order to study the composition and the effects of aqueoussoluble constituents from Pleurotus ostreato-roseus Sing. on mice serum proteins, the P. ostreato-roseus strain POR-020 was cultured on powered grass-sugarcane bagasse-sawdust-wheat bran-substrate, and fruiting bodies were produced, milled, extracted with ethanol and hot water, and fractional precipitated with ethanol producing two fractions, FI-1 and FI-2. These fractions showed, respectively, 47.87 and 26.45% (d. m.) carbohydrate, and 2.07 and 1.97% (d.m.) protein composition. The fractions were administered i.p. in AJ male mice in a singledose of 257 mg/kg (FI-1) and 31.5 mg/kg (FI-2). After 15 days administration, electrophoretic analysis of serum proteins detected that FI-1 increased 50, 25, 0.35, and 28.6% in b-, a1-, a2- and g-globulin regions, and FI-2 increased 4.4 and 21 % in band a1-globulin, respectively.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

• 1 Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Current Medicinal Chemistry 2000; 7:715-29
• 2 Singer R. The agaricales in modern taxonomy. 4th ed. FDR: Koeltz Scientific Books, 1986
• 3 Zhang J, Wang G, Li H, Zhuang C, Mizuno T, Ito H, Suzuki C, Okamoto H, Li J. Antitumor polysaccharide from a Chinese mushroom, Yuhuangno, the fruiting body of Pleurotus citrinopileatus Bioscience and Biotechnological Biochemistry 1994. 58:1295-201
• 4 Zhuang C, Mizuno T, Shimada A, Ito H, Suzuki C, Mayazumi Y, Okamoto H, Ma Y, Li J. Antitumor protein-containing polysaccharides from a chinese mushroom Fengweigu or Houbitake, Pleurotus sajor-caju (Fr.) Sing. Bioscience and Biotechnological Biochemistry 1993. 57:901-6
• 5 Maeda YY, Chihara G, Ishimura K. Unique increase of serum protein and action of antitumor polysaccharides. Nature 1974. 252:250-2
• 6 Mizuno T, Ando M, Sugie R, Ito H, Shimura K, Sumiya T, Matsuura A. Antitumor activity of some polysaccharides isolated from an edible mushroom, Ningyotake, the fruiting body and the cultured mycelium of Polyporus confluens Bioscience and Biotechnological Biochemistry 1992. 56:34-41
• 7 Bradford MM. A rapid and sensitive method for the quantitation of micrograms of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976. 72:248-54
• 8 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry 1956. 28:350-6
• 9 Laemmli UK. Cleavage of structural protein during assembly of head of bacteriophage T-4. Nature 1970

Characterization of fractions of aqueoussoluble constituents of the mushroom Pleurotus ostreato-roseus Sing. and its effects on the serum protein profile of mice

Nerivan Barbosa de Lima^I; Maria Raquel Querino de Sousa^I; Onaldo Guedes Rodrigues^II; Marcos José Correia^II

Publication Dates

• Publication in this collection
  05 Oct 2009
• Date of issue
  2002