Abstract
Electrochemical methods are powerful in the characterization and design of redox-modulating agents. We, herein, report the electrochemical investigation, in aprotic medium, of eleven synthetic 3-thio-substituted-nor-beta-lapachones, along with the determination of cytotoxic activity and correspondent selectivity index, against several cancer cell lines and one normal cell. Four of the quinones are novel compounds. The redox behavior is representative of two independent systems: the easy reduction of the quinone moiety and, at far more negative potential, the reductive cleavage of the C-S-C bonding; and the anodic part controlled by the oxidation of the sulfur moiety. The compounds have shown relevant cytotoxic activity, with emphasis on 3-phenyl-thio-2,2-dimethyl-2,3-dihydronaphtho[1,2-b]furan-4,5-dione (compound 2), which mechanism of molecular action was shown to be related to reactive oxygen species (ROS) release. Despite the absence of a linear correlation, there is a trend: the majority of the thionaphthoquinones, with values of first wave reduction potential, less negative than -0.65 V, were active. The less electrophilic compound (3-(cyclohexylthio)-2,2-dimethyl-2,3-dihydronaphtho[1,2-b]furan-4,5-dione, cyclohexyl derivative) is also the less cytotoxic toward cancer cells. Agents containing chalcogens and quinones can be used to attack entities with a disturbed redox balance.
Keywords:
thionaphthoquinones; electrochemical parameters; cancer; electrodic mechanism; ROS release; structure-electroactivity-biological activity relationship
Introduction
Redox processes, central to life, pervade practically all fundamental bioprocesses, from bioenergetics to metabolism and life functions.11 Sies, H.; Berndt, C.; Jones, D. P.; Annu. Rev. Biochem. 2017, 86, 715.
A disturbed intracellular redox balance, resulting in oxidative stress (OS), has been considered as the biochemical basis for various human diseases, including different types of cancer.22 Sies, H.; Jones, D. P. In Encyclopedia of Stress, vol. 3; Fink, G., ed.; Elsevier: Amsterdam, 2007, p. 45.,33 Valko, M.; Leibfritz, D.; Moncol, J.; Cronin, M. T. D.; Mazura, M.; Telser, J.; Int. J. Biochem. Cell Biol. 2007, 39, 44. OS is an imbalance between oxidants, mainly reactive oxygen species (ROS), and antioxidants, in favor of the oxidants, leading to a disruption of redox signaling and control and/or molecular damage.11 Sies, H.; Berndt, C.; Jones, D. P.; Annu. Rev. Biochem. 2017, 86, 715.,22 Sies, H.; Jones, D. P. In Encyclopedia of Stress, vol. 3; Fink, G., ed.; Elsevier: Amsterdam, 2007, p. 45.
Some cancer cells proliferate under conditions of high-intensity OS,11 Sies, H.; Berndt, C.; Jones, D. P.; Annu. Rev. Biochem. 2017, 86, 715. compared with normal cells, because of their accelerated metabolism. ROS may cause damage to biological macromolecules, such as DNA, lipids and proteins, with noxious consequences to cells. Compared to non-cancerous cells, ROS levels are considerably closer to the critical redox threshold at which cell death is induced.44 Pelicano, H.; Carney, D.; Huang, P.; Drug Resist. Updates
2004, 7, 97.
5 Viswanathan, U. M.; Burkholz, T.; Jacob, C. Z.; Phys. Chem.
2013, 227, 691.-66 Jamier, V.; Ba, L. A.; Jacob, C.; Chem. - Eur. J.
2010, 16, 10920. Normal cells often can tolerate a certain level of exogenous ROS, whereas cancer cells cannot.77 Trachootham, D.; Alexandre, J.; Huang, P.; Nat. Rev. Drug Discovery
2009, 8, 579. These pre-existing biochemical differences between healthy and malignant tissues are significant and are used in the design of selective redox chemotherapeutic drugs.66 Jamier, V.; Ba, L. A.; Jacob, C.; Chem. - Eur. J.
2010, 16, 10920.
7 Trachootham, D.; Alexandre, J.; Huang, P.; Nat. Rev. Drug Discovery
2009, 8, 579.
8 Cabello, C. M.; Bair, W. B.; Wondrak, G. T.; Curr. Opin. Invest. Drugs
2007, 8, 1022.
9 Wondrak, G. T.; Antioxid. Redox Signaling
2009, 11, 3013.
10 Fry, F. H.; Holme, A. L.; Giles, N. M.; Giles, G. I.; Collins, C.; Holt, K.; Pariagh, S.; Gelbrich, T.; Hursthouse, M. B.; Gutowski, N. J.; Jacob, C.; Org. Biomol. Chem. 2005, 3, 2579.-1111 Giles, N. M.; Gutowski, N. J.; Giles, G. I.; Jacob, C.; FEBS Lett. 2003, 535, 179.
Some anticancer redox drugs have the ability to alter cellular redox status, by redox cycling-mechanism related to the reversible single electron transfer from a cellular reducing agent (e.g., glutathione or nicotinamide adenine dinucleotide phosphate (NAD(P)H)) or other chemicals onto oxygen, with the production of ROS.1111 Giles, N. M.; Gutowski, N. J.; Giles, G. I.; Jacob, C.; FEBS Lett. 2003, 535, 179. After electron transfer to oxygen, the redox catalyst is regenerated by spontaneous or enzyme-driven redox reaction.1111 Giles, N. M.; Gutowski, N. J.; Giles, G. I.; Jacob, C.; FEBS Lett. 2003, 535, 179.
The impressive number of ongoing clinical trials that examine the therapeutic performance of novel redox drugs in cancer patients demonstrates that redox chemotherapy has attracted deep interest in the scientific community.88 Cabello, C. M.; Bair, W. B.; Wondrak, G. T.; Curr. Opin. Invest. Drugs 2007, 8, 1022.,99 Wondrak, G. T.; Antioxid. Redox Signaling 2009, 11, 3013.
Among these catalysts, compounds containing a chalcogen (ROS modulator) and a quinone (ROS generator), are particularly well suited to respond to the presence of OS,55 Viswanathan, U. M.; Burkholz, T.; Jacob, C. Z.; Phys. Chem.
2013, 227, 691.,1010 Fry, F. H.; Holme, A. L.; Giles, N. M.; Giles, G. I.; Collins, C.; Holt, K.; Pariagh, S.; Gelbrich, T.; Hursthouse, M. B.; Gutowski, N. J.; Jacob, C.; Org. Biomol. Chem. 2005, 3, 2579.
11 Giles, N. M.; Gutowski, N. J.; Giles, G. I.; Jacob, C.; FEBS Lett. 2003, 535, 179.
12 Shaaban, S.; Diestel, R.; Hinkelmann, B.; Muthukumar, Y.; Verma, R. P.; Sasse, F.; Jacob, C.; Eur. J. Med. Chem. 2012, 58, 192.
13 Doering, M.; Diesel, B.; Gruhlke, M. C. H.; Viswanathan, U. M.; Mániková, D.; Chovanec, M.; Burkholz, T.; Slusarenko, A. J.; Kiemer, A. K.; Jacob, C.; Tetrahedron
2012, 68, 10577.
14 Vieira, A. A.; Brandão, I. R.; Valença, W. O.; de Simone, C. A.; Cavalcanti, B. C.; Pessoa, C.; Carneiro, T. R.; Braga, A. L.; da Silva Jr., E. N.; Eur. J. Med. Chem. 2015, 101, 254.
15 Giles, G. I.; Tasker, K. M.; Johnson, R. J. K.; Jacob, C.; Peers, C.; Green, K. N.; Chem. Commun. 2001, 2490.
16 Giles, G. I.; Giles, N. M.; Collins, C. A.; Holt, K.; Fry, F. H.; Lowden, P. A. S.; Gutowski, N. J.; Jacob, C.; Chem. Commun.
2003, 2030.-1717 Jardim, G. A. M.; da Cruz, E. H. G.; Valença, W. O.; Lima, D. J. B.; Cavalcanti, B. C.; Pessoa, C.; Rafique, J.; Braga, A. L.; Jacob, C.; da Silva Jr., E. N.; da Cruz, E. H. G.; Molecules
2018, 23, 83. being interesting for the development of anti-cancer drugs. Their mechanisms of action depend on the environment, as they have the possibility to function as antioxidant in normal cells1111 Giles, N. M.; Gutowski, N. J.; Giles, G. I.; Jacob, C.; FEBS Lett. 2003, 535, 179. and as pro-oxidants in cells exposed to OS.1414 Vieira, A. A.; Brandão, I. R.; Valença, W. O.; de Simone, C. A.; Cavalcanti, B. C.; Pessoa, C.; Carneiro, T. R.; Braga, A. L.; da Silva Jr., E. N.; Eur. J. Med. Chem. 2015, 101, 254.,1717 Jardim, G. A. M.; da Cruz, E. H. G.; Valença, W. O.; Lima, D. J. B.; Cavalcanti, B. C.; Pessoa, C.; Rafique, J.; Braga, A. L.; Jacob, C.; da Silva Jr., E. N.; da Cruz, E. H. G.; Molecules
2018, 23, 83. If these agents are also able to adjust their activity in response to their redox environment in vivo, they might provide access to an important category of drugs.
Electrochemical methods are extraordinarily powerful and useful, in the characterization and design of redox-modulating agents.55 Viswanathan, U. M.; Burkholz, T.; Jacob, C. Z.; Phys. Chem.
2013, 227, 691.,1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun.
2008, 2612.
19 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.
20 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252.-2121 Ferreira, D. C. M.; Tapsoba, I.; Arbault, S.; Bouret, Y.; Moreira, M. S. A.; Pinto, A. V.; Goulart, M. O. F.; Amatore, C.; ChemBioChem
2009, 10, 528. They provide thermodynamic and kinetic molecular parameters, under different conditions that may be related to their biological activity in living cells and appear well adapted to explore redox pathways in vitro and the related in vivo studies.2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252.,2121 Ferreira, D. C. M.; Tapsoba, I.; Arbault, S.; Bouret, Y.; Moreira, M. S. A.; Pinto, A. V.; Goulart, M. O. F.; Amatore, C.; ChemBioChem
2009, 10, 528. Thus, they have high relevance in the field of redox medicine.
As such, the present work focused on the electrochemical investigation, in aprotic medium, of eleven synthetic 3-substituted thio-nor-beta-lapachones (2-12) (Figure 1), which syntheses have been reported previously,2222 da Silva Jr., E. N.; Souza, M. C. B. V.; Fernandes, M. C.; Menna-Barreto, R. F. S.; Pinto, M. C. F. R.; Lopes, F. A.; Simone, C. A.; Andrade, C. K. Z.; Pinto, A. V.; Ferreira, V. F.; Castro, S. L.; Bioorg. Med. Chem. 2008, 16, 5030. except for 6, 10, 11 and 12, which are novel compounds. We also, herein, present electroanalysis of their interaction with oxygen, along with the determination of cytotoxic activity against four cancer cell lines and a normal cell, in search for adequate selectivity index and aiming to correlating electrochemical and biological data. The mechanism of action based on ROS release is evaluated for the most active compound. The electrochemical features and biological activities of the precursor, nor-beta-lapachone (1), are included for comparison purposes.
Chemical structures of the nor-beta-lapachone (1), studied thionaphthoquinones (2-12) and beta-lapachone (13). Ac: acetyl.
Nor-beta-lapachone (1), a smaller homolog of beta-lapachone (13), can be easily prepared from nor-lapachol2222 da Silva Jr., E. N.; Souza, M. C. B. V.; Fernandes, M. C.; Menna-Barreto, R. F. S.; Pinto, M. C. F. R.; Lopes, F. A.; Simone, C. A.; Andrade, C. K. Z.; Pinto, A. V.; Ferreira, V. F.; Castro, S. L.; Bioorg. Med. Chem. 2008, 16, 5030. by acid catalysis.2222 da Silva Jr., E. N.; Souza, M. C. B. V.; Fernandes, M. C.; Menna-Barreto, R. F. S.; Pinto, M. C. F. R.; Lopes, F. A.; Simone, C. A.; Andrade, C. K. Z.; Pinto, A. V.; Ferreira, V. F.; Castro, S. L.; Bioorg. Med. Chem. 2008, 16, 5030.,2323 Cardoso, M. F. C.; da Silva, I. M. C. B.; dos Santos Jr., H. M.; Rocha, D. R.; Araújo, A. J.; Pessoa, C.; de Moraes, M. O.; Lotufo, L. V. C.; da Silva, F. C.; Santos, W. C.; Ferreira, V. F.; J. Braz. Chem. Soc. 2013, 24, 12. Sulfur has a variety of oxidation states and is highly versatile in function,2424 Jacob, C.; Giles, G. I.; Giles, N. M.; Sies, H.; Angew. Chem., Int. Ed. 2003, 42, 4742. thus, the interest in their synthetic coupling.
Other chalcogen-containing beta-lapachone's (13) scaffolds were synthesized and screened as cytotoxic compounds against several cancer cell lines, and some of them had been shown to be highly active and selective.1414 Vieira, A. A.; Brandão, I. R.; Valença, W. O.; de Simone, C. A.; Cavalcanti, B. C.; Pessoa, C.; Carneiro, T. R.; Braga, A. L.; da Silva Jr., E. N.; Eur. J. Med. Chem. 2015, 101, 254.,2525 da Cruz, E. H. G.; Silvers, M. A.; Jardim, G. A. M.; Resende, J. M.; Cavalcanti, B. C.; Bomfim, I. S.; Pessoa, C.; de Simone, C. A.; Botteselle, G. V.; Braga, A. L.; Nair, D. K.; Namboothiri, I. N. N.; Boothman, D. A.; da Silva Jr., E. N.; Eur. J. Med. Chem. 2016, 122, 1. Additional reports on antitumor thio-substituted quinones had appeared in the literature.1212 Shaaban, S.; Diestel, R.; Hinkelmann, B.; Muthukumar, Y.; Verma, R. P.; Sasse, F.; Jacob, C.; Eur. J. Med. Chem. 2012, 58, 192.,2626 Guimarães, E.; Hussene, C.; Dias, G. G.; Diogo, E. B. T.; de Melo, I. M. M.; Rodrigues, B. L.; Valença, W. O.; Camara, C. A.; da Silva, M. G.; de Oliveira, R. N.; de Paiva, Y. G.; Goulart, M. O. F.; Cavalcanti, B. C.; Pessoa, C.; da Silva Jr., E. N.; Bioorg. Med. Chem. 2014, 22, 1608.,2727 Bhasin, D.; Chettiar, S. N.; Etter, J. P.; Mok, M.; Li, P. K.; Bioorg. Med. Chem. 2013, 21, 4662. Antimalarial and trypanocidal activities for this class of hybrid compounds were also reported.2222 da Silva Jr., E. N.; Souza, M. C. B. V.; Fernandes, M. C.; Menna-Barreto, R. F. S.; Pinto, M. C. F. R.; Lopes, F. A.; Simone, C. A.; Andrade, C. K. Z.; Pinto, A. V.; Ferreira, V. F.; Castro, S. L.; Bioorg. Med. Chem. 2008, 16, 5030.,2828 Sharma, A.; Santos, I. O.; Gaur, P.; Ferreira, V. F.; Garcia, C. R. S.; da Rocha, D. R.; Eur. J. Med. Chem. 2013, 59, 48.,2929 da Silva, F. C.; Ferreira, V. F.; Curr. Org. Synth. 2016, 13, 334.
Up to our knowledge, there are few electrochemical reports on thionaphthoquinones,1515 Giles, G. I.; Tasker, K. M.; Johnson, R. J. K.; Jacob, C.; Peers, C.; Green, K. N.; Chem. Commun. 2001, 2490.,1919 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.,2828 Sharma, A.; Santos, I. O.; Gaur, P.; Ferreira, V. F.; Garcia, C. R. S.; da Rocha, D. R.; Eur. J. Med. Chem. 2013, 59, 48.,3030 Ibis, C.; Ayla, S. S.; Yavuz, S.; Phosphorus, Sulfur Silicon Relat. Elem. 2014, 189, 492.
31 Ibis, C.; Ayla, S. S.; Asar, H.; Synth. Commun. 2014, 44, 121.
32 Ibis, C.; Shntaif, A. H.; Bahar, H.; Ayla, S. S.; J. Serb. Chem. Soc
2015, 80, 731.-3333 Walsh, J. S.; Miwa, G. T.; Annu. Rev. Pharmacol. Toxicol. 2011, 51, 145. especially in aprotic medium.1919 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.,2828 Sharma, A.; Santos, I. O.; Gaur, P.; Ferreira, V. F.; Garcia, C. R. S.; da Rocha, D. R.; Eur. J. Med. Chem. 2013, 59, 48.,3232 Ibis, C.; Shntaif, A. H.; Bahar, H.; Ayla, S. S.; J. Serb. Chem. Soc
2015, 80, 731.
33 Walsh, J. S.; Miwa, G. T.; Annu. Rev. Pharmacol. Toxicol. 2011, 51, 145.-3434 Ferreira, F. R.; Ferreira, S. B.; Araújo, A. J.; Marinho Filho, J. D. B.; Pessoa, C.; Moraes, M. O.; Costa-Lotufo, L. V.; Montenegro, R. C.; Silva, F. C.; Ferreira, V. F.; da Costa, J. G.; de Abreu, F. C.; Goulart, M. O. F.; Electrochim. Acta
2013, 110, 634. Most of the quinones and their intermediates, as well as reduced forms, exist inside hydrophobic cell membranes. As such, the aprotic polar dimethylformamide (DMF), dimethyl sulfoxide (DMSO) or acetonitrile are the solvents of choice, for electrochemical investigation. This is justified: (i) due to the necessity to mimic the nonpolar environment in the cell1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun.
2008, 2612.
19 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.-2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252.,3232 Ibis, C.; Shntaif, A. H.; Bahar, H.; Ayla, S. S.; J. Serb. Chem. Soc
2015, 80, 731.,3333 Walsh, J. S.; Miwa, G. T.; Annu. Rev. Pharmacol. Toxicol. 2011, 51, 145. and (ii) to allow a single-electron transfer to generate the semiquinone,1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun.
2008, 2612.
19 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.-2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252. which is better obtained in absence of protons.
Based on the ever-increasing interest on biologically active quinones, with new strategies to increase selectivity, directing the compound to the target,3535 de Abreu, F. C.; Ferraz, P. A. L.; Goulart, M. O. F.; J. Braz. Chem. Soc.
2002, 13, 19. the excitement toward their syntheses and knowledge of redox mechanisms continues. Theoretical and experimental approaches, together with evaluation of redox-based biological activity, in search for better prototypes, in the continuous fight against cancer and tropical diseases1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun.
2008, 2612.
19 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.-2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252. are welcome.
Experimental
Reagents
Extra-dry N,N-dimethylformamide (DMF, 99.8%) was acquired from Acros Organics (Geel, Belgium), tetrabutylammonium hexafluorophosphate (TBAPF6) and tetra-N-butylammonium fluoride (TBAP) and the other chemicals from Sigma-Aldrich (St. Louis, USA). TBAPF6 was purified by recrystallization from absolute ethanol (×3), and dried at reduced pressure, at 60 ºC. All the reagents were analytical grade. All the solutions were prepared using ultrapure water (18.2 MΩ cm) from Milli-Q (Millipore Inc., Ziefen, Switzerland).
Electrochemical studies
Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) experiments were performed with a conventional three electrode cell in an Autolab PGSTAT-30 potentiostat (Echo Chemie, Utrecht, The Netherlands) coupled to a PC microcomputer, using GPES 4.9 software.
Reduction and oxidation studies
The working electrode was a glassy carbon electrode (GCE) BAS (d = 3 mm), the counter electrode was a Pt wire and the reference electrode was an Ag|AgCl, Cl- (sat.). All electrodes were contained in a one-compartment electrochemical cell with a volumetric capacity of 10 mL. GCE was cleaned up by polishing with alumina on a polishing felt (BAS polishing kit). In CV experiments, the scan rate varied from 10 to 500 mV s-1. Electrochemical reduction/oxidation was performed in aprotic media (DMF + TBAPF6, 0.1 mol L-1), at room temperature (25 ± 2 °C). Each compound (1 × 10-3 mol L-1) was added to the supporting electrolyte, and the solution was deoxygenated with argon before each CV measurement. The quinones do not adsorb on the surface. It is only necessary to flush argon on the electrode surface between the runs. Different potential ranges were used from the cathodic to anodic scan. The most representative range was from 0 to -3.0 V vs. Ag|AgCl, Cl− (sat.), in the cathodic direction and 0 to +1.2 V, in the anodic direction. For the DPV measurements, the pulse amplitude was 50 mV, the pulse width, 70 ms and the scan rate, 5 mV s-1.2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem 2016, 3, 2252.,2626 Guimarães, E.; Hussene, C.; Dias, G. G.; Diogo, E. B. T.; de Melo, I. M. M.; Rodrigues, B. L.; Valença, W. O.; Camara, C. A.; da Silva, M. G.; de Oliveira, R. N.; de Paiva, Y. G.; Goulart, M. O. F.; Cavalcanti, B. C.; Pessoa, C.; da Silva Jr., E. N.; Bioorg. Med. Chem. 2014, 22, 1608.
Experiments in the presence of oxygen
To investigate the reactivity of the thionaphthoquinones (2-12) toward oxygen, electrochemical reductions, in aprotic medium (DMF + TBAP, 0.1 mol L-1), were performed in the presence and absence of oxygen. Oxygen was bubbled into the cell and its concentration was monitored by using an oxygen meter (Digimed DM-4, São Paulo, Brazil). CVs were recorded at different oxygen concentrations. The parameters analyzed were the anodic shift in the potential of the first reduction wave (EpIc) and the current increase at the same peak (IpIc) or decrease in the correspondent anodic peak (IpIa).2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252.,3434 Ferreira, F. R.; Ferreira, S. B.; Araújo, A. J.; Marinho Filho, J. D. B.; Pessoa, C.; Moraes, M. O.; Costa-Lotufo, L. V.; Montenegro, R. C.; Silva, F. C.; Ferreira, V. F.; da Costa, J. G.; de Abreu, F. C.; Goulart, M. O. F.; Electrochim. Acta
2013, 110, 634.
35 de Abreu, F. C.; Ferraz, P. A. L.; Goulart, M. O. F.; J. Braz. Chem. Soc.
2002, 13, 19.
36 Goulart, M. O. F.; Ossowski, T.; Pipka, P.; Liwo, A.; Bioelectrochemistry
2003, 59, 85.-3737 Goulart, M. O. F.; Lima, N. M. F.; Santana, A. E. G.; Ferraz, P. A. L.; Cavalcanti, J. C. M.; Liwo, A.; Falkowsky, P.; Ossowsky, T.; J. Electroanal. Chem.
2004, 566, 25. The apparent association constant for the reaction with oxygen (kap) can be determined by equation 1.3838 Bard, A. J.; Faulkner, L. R.; Electrochemical Methods: Fundamentals and Applications; Wiley: New York, 2001.
where, Ipc = catalytic current; IpR/Ip0 = normalized current; kap = apparent catalytic constant (s-1); n = scan rate (V s-1); ν = number of electrons; F = Faraday constant (96485 C mol-1); T = temperature (298 K) and R = 8.314 J mol-1 K-1.
Synthesis
The reagents were purchased from Sigma-Aldrich (St. Louis, USA) and were used without further purification. Column chromatography was performed with silica gel 60 (Merck, 70-230 mesh). Analytical thin layer chromatography was performed with silica gel plates (Merck, Darmstadt, Germany, TLC silica gel 60 F254), and the plates were visualized using UV light. The indicated yields refer to homogeneous materials purified by chromatography and confirmed by spectroscopic techniques. Melting points were obtained on a Thermo scientific 9100 apparatus (Waltham, MA, USA) and were uncorrected. Infrared spectra were collected using KBr pellets on a PerkinElmer model 1420 FTIR spectrophotometer (Palo Alto, USA), and the spectra were calibrated relative to the 1601.8 cm-1 absorbance of polystyrene. 1H and 13C nuclear magnetic resonance (NMR) were recorded at room temperature using a Varian Mercury 300 or Varian Mercury 400 MHz (Quebec, Canada), in DMSO-d6. The chemical shift data were reported in units of δ (ppm) downfield from solvent, and the solvent was used as an internal standard; coupling constants (J) are reported in hertz and refer to apparent peak multiplicities. High-resolution mass spectra (HRMS) were recorded on a MICROMASS Q-TOF mass spectrometer (Waters, Oconomowoc, WI, USA).
Compounds 6, 10-12 were synthesized for the first time and spectral data reported in the Supplementary Information section (SI, Figures S1-S4).
General procedure for the preparation of 2-12
In a 125 mL flask containing a solution of nor-lapachol (14) (500 mg, 2.2 mmol) in 40 mL dry chloroform, externally cooled with ice and under an inert atmosphere, bromine (4.4 mL, 13.7 g, 8.59 mol) was added. A red solid precipitate of the cationic ortho-quinone methide intermediate immediately formed, and the reaction mixture was left, under stirring conditions, for additional 10 min. The excess bromine was removed under reduced pressure and 40 mL of chloroform was immediately added. The mixture was, then, externally cooled, in an ice bath. To this solution, a dry chloroform solution containing the appropriate thiol nucleophile (4.4 mmol in 25 mL), was slowly added. The reaction mixture was stirred for 3 h and, then, poured into 50 mL of distilled water. The phases were separated and the organic phase washed with saturated sodium bicarbonate solution (3 × 50 mL), distilled water (3 × 50 mL), dried with anhydrous sodium sulfate, filtered and evaporated under reduced pressure. The resulting red solid, which was purified by flash column chromatography on silica gel, was eluted with gradient mixtures of hexane and ethyl acetate. The protocols for preparing 2-5 and 7-9 and the physical and spectroscopic data were previously reported.2222 da Silva Jr., E. N.; Souza, M. C. B. V.; Fernandes, M. C.; Menna-Barreto, R. F. S.; Pinto, M. C. F. R.; Lopes, F. A.; Simone, C. A.; Andrade, C. K. Z.; Pinto, A. V.; Ferreira, V. F.; Castro, S. L.; Bioorg. Med. Chem. 2008, 16, 5030.
3-(4-(Methylthio)phenyl)thio-2,2-dimethyl-2,3-dihydronaphtho[1,2-b]furan-4,5-dione (6)
Isolated as an orange solid, in 83% yield; mp 201-203 ºC; IR (KBr) ν / cm-1 2923, 1658, 1645, 1614, 1570, 1476, 1400, 1251, 1224, 1093, 1009, 791 (Figure S1, SI section); 1H NMR (CDCl3, 500 MHz) 1.51 (3H, s, (C-2)-CH3), 1.81 (3H, s, (C-2)-CH3), 2.43 (3H, s), 4.47 (1H, s), 7.13 (2H, d, J 8.5 Hz), 7.47 (2H, d, J 8.5 Hz), 7.57-7.67 (1H, m), 7.57-7.67 (1H, m), 7.57-7.67 (1H, m), 8.08 (1H, d, J 7.0 Hz) (Figure S2, SI section); 13C NMR (CDCl3, 125 MHz) δ 15.7, 24.5, 28.7, 59.1, 95.9, 115.8, 125.1, 126.9, 127.6, 129.6, 131.1, 131.2, 132.4, 133.8, 134.6, 139.0, 168.5, 174.7, 180.7 (Figure S3, SI section); HRMS (electrospray ionization, ESI) m/z, [M + Na]+ calcd. for C21H18O3S2Na+: 405.0590; found: 405.0594. Δ = 1.0 ppm (Figure S4, SI section).
3-(Benzylthio)-2,2-dimethyl-2,3-dihydronaphtho [1,2-b]furan-4,5-dione (10)
Compound 10 was isolated as an orange solid in 88% yield; mp 186-188 ºC; IR (KBr) ν / cm-1 1655, 1644, 1610, 1585, 1568, 1403, 1247, 1223, 1082 (Figure S5, SI section); 1H NMR (CDCl3, 500 MHz) δ 1.38 (3H, s), 1.61 (3H, s), 3.99 (2H, d, J 10.2 Hz), 4.09 (1H, s), 7.21-7.40 (3H, m), 7.38 (2H, d, J 7.5 Hz), 7.55-7.65 (1H, m), 7.55-7.65 (1H, m), 7.55-7.65 (1H, m), 8.07 (1H, d, J 7.8 Hz) (Figure S6, SI section); 13C NMR (CDCl3, 125 MHz) δ 24.3, 28.5, 37.5, 53.1, 95.4, 117.8, 124.9, 127.3, 127.7, 128.6, 129.3, 129.5, 131.1, 132.5, 132.3, 134.6, 138.1, 168.2, 175.1, 180.9 (Figure S7, SI section); HRMS (ESI) m/z, [M + Na]+ calcd. for C21H18O3SNa+: 373.0869; found: 373.0883. Δ = 3.7 ppm (Figure S8, SI section).
3-(Cyclohexylthio)-2,2-dimethyl-2,3-dihydronaphtho [1,2-b]furan-4,5-dione (11)
Compound 11 was isolated as an orange solid, in 81% yield; mp 155-156 ºC; IR (KBr) ν / cm-1 2931, 2849, 1654, 1644, 1614, 1587, 1569, 1449, 1399, 1248, 1219, 1079 (Figure S9, SI section); 1H NMR (CDCl3, 500 MHz) δ 1.20-1.40 (6H, m), 1.56 (3H, s), 1.71 (3H, s), 1.74-1.80 (2H, m), 1.96-2.11 (2H, m), 3.06-3.15 (1H, m), 4.16 (1H, s), 7.55-7.65 (1H, m), 7.55-7.65 (1H, m), 7.55-7.65 (1H, m), 8.08 (1H, dt, J 7.3 and 1.1 Hz) (Figure S10, SI section); 13C NMR (CDCl3, 125 MHz) δ 24.4, 25.7, 25.9, 26.1, 28.4, 33.4, 34.5, 45.1, 52.2, 95.2, 118.3, 124.7, 127.7, 129.3, 130.9, 132.0, 134.4, 167.8, 175.0, 180.9 (Figure S11, SI section); HRMS (ESI) m/z, [M + Na]+ calcd. for C20H22O3SNa+: 365.1182; found 365.1193. Δ = 3.0 ppm (Figure S12, SI section).
S-(2,2-Dimethyl-4,5-dioxo-2,3,4,5-tetrahydronaphtho [1,2-b]furan-3-yl)ethanethioate (12)
Compound 12 was isolated as an orange solid in 85% yield; mp 211-213 ºC; IR (KBr) ν / cm-1 1656, 1645, 1612, 1586, 1569, 1492, 1400, 1223, 1080, 788 (Figure S13, SI section); 1H NMR (CDCl3, 500 MHz) δ 1.18 (3H, s), 1.75 (3H, s), 2.04 (3H, s), 5.16 (1H, s), 7.71-7.80 (1H, m), 7.71-7.80 (1H, m), 7.71-7.80 (1H, m), 8.18 (1H, d, J 8.3 and 1.5 Hz) (Figure S14, SI section); 13C NMR (CDCl3, 125 MHz) ゴ 20.9, 21.2, 27.8, 66.9, 96.0, 111.3, 125.7, 126.6, 130.0, 131.5, 133.5, 134.9, 170.9, 171.4, 174.6, 180.1 (Figure S15, SI section); HRMS (ESI) m/z, [M + Na]+ calcd. for C16H14O4SNa+: 325.0505; found 325.0515. ν = 3.1 ppm (Figure S16, SI section).
Evaluation of the cytotoxic activity by an in vitro assay
Cell lines and culture
All the compounds were evaluated in vitro using the MTT assay against four cancer cell lines: SF295 (human glioblastoma), HCT-116 (human colon carcinoma), PC3 (prostate) and K562 (leukemia). Doxorubicin was used as a positive control. The selectivity of the compounds toward a normal proliferating cell line was investigated using the Alamar Blue assay with human peripheral blood mononuclear cells (PBMC), after 72 h of compound exposure. All cancer cell lines and PBMC were maintained in RPMI 1640 medium. All culture media were supplemented with 20% (PBMC) or 10% (SF-295, HCT-116, PC3 and K562) fetal bovine serum, 2 mmol L-1 L-glutamine, 100 IU mL-1 penicillin, 100 µg mL-1 streptomycin, at 37 °C with 5% CO2. PBMC cultures were also supplemented with 2% phytohaemagglutinin. In the cytotoxicity experiments, cells were plated in 96-well plates (0.1 × 106 cells per well for PC3 and SF-295 cells, 0.3 × 106 cells per well, 0.7 × 105 cells per well for HCT-116 cells, and 1 × 106 cells per well for PBMC). All tested compounds were dissolved in DMSO. The final concentration of DMSO in the culture medium was kept constant (0.1%, v/v).
Determination of cytotoxicity
The cell viability was determined by the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H-tetrazolium bromide (MTT) to a blue formazan product, as described.3939 Mosmann, T.; J. Immunol. Methods 1983, 65, 55. At the end of the incubation time (72 h), the plates were centrifuged and the medium was replaced with fresh medium (200 mL) containing 0.5 mg mL-1 MTT. Three hours later, the MTT formazan product was dissolved in DMSO (150 µL) and the absorbance was measured using a multiplate reader (DTX 880 Multimode Detector, Beckman Coulter, Inc. Fullerton, California, EUA). The compound effect was quantified as the percentage of control absorbance of the reduced dye at 595 nm. The viability for PBMC cell was determined by the method of Alamar Blue. This incorporates a fluorometric/colorimetric growth indicator based on detection of metabolic activity.4040 Hamid, R.; Rotshteyn, Y.; Rabadi, L.; Parikh, R.; Bullock, P.; Toxicol. In Vitro 2004, 18, 703. After 24 h, compounds were added to each well, and the cells were incubated for 72 h. Twenty four hours before the end of the incubation, 10 µL of stock solution (0.312 mg mL-1) of Alamar Blue were added to each well. The absorbance was measured at 570 (reduced) and 595 nm. All cell treatments were carried out with two replicates performed in triplicate. Values were computed using GraphPad Prism® 5.0 program.4141 GraphPad Prism 5.0; GraphPad Software Inc., San Diego, USA, 2007.
Measurement of reactive oxygen species generation
Intracellular reactive oxygen species (ROS) accumulation was monitored using 5-(6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H2DCFDA), which is converted to the highly fluorescent dichlorofluorescein (DCF) in the presence of intracellular ROS.4242 LeBel, C. P.; Ischiropoulos, H.; Bondy, S. C.; Chem. Res. Toxicol. 1992, 5, 227. HCT-116 cells were pre-loaded with 10 µmol L-1 CM-H2DCFDA and incubated for 40 min in the dark at 37 °C/5% CO2. After that time, the medium containing CM-H2DCFDA was removed and the cells were washed twice with phosphate-buffered saline (PBS) buffer. From this stage, the cells were always protected from light. Fresh medium containing the compounds was then added and incubated at the times of interest (30 min, 1 and 3 h). Cells were then harvested, washed and re-suspended in PBS and immediately analyzed by flow cytometry, using excitation and emission wavelengths of 490 and 525 nm, respectively. Menadione (2-methyl-1,4-naphthoquinone) (20 µmol L-1) and hydrogen peroxide (H2O2, 100 µmol L-1) were used as positive controls. A total of 5,000 events were analyzed per sample. Data are expressed as mean ± standard error of mean (SEM), from two independent experiments.
Results and Discussion
Chemistry
Initially, the synthesis of thionaphthoquinones 2-12, previously explored by our research group,2222 da Silva Jr., E. N.; Souza, M. C. B. V.; Fernandes, M. C.; Menna-Barreto, R. F. S.; Pinto, M. C. F. R.; Lopes, F. A.; Simone, C. A.; Andrade, C. K. Z.; Pinto, A. V.; Ferreira, V. F.; Castro, S. L.; Bioorg. Med. Chem. 2008, 16, 5030. involved the formation in situ of ortho-quinone methide (o-QM) by reaction between nor-lapachol (14), in dry chloroform, and bromine under low temperature (ice bath) and nitrogen atmosphere. A red solid precipitate of the cationic .-QM intermediate immediately formed was reacted with R-SH compound, resulting in a red solid, which was purified by flash column chromatography on silica gel (Figure 2). The structures of the synthesized compounds were confirmed by spectroscopic techniques (see SI section), such as 1H and 13C NMR, infrared spectroscopy and HRMS-ESI.
Scheme and chemical yields for the synthesis of thionaphthoquinones 2-12. (a) Br2, CHCl3; (b) R−SH. Bn = benzyl; Cy = cyclohexyl; Ac = acetyl.
The analysis of 1H NMR spectrum of compound 6 shows a signal at 4.47 ppm from the methine hydrogen and the typical benzoaromatic pattern of 1,2-naphthoquinone isomers as three signals at 7.57-7.67 (1H, m, H-7 and H-8), 7.57-7.67 (1H, m, H-9), 8.08 (1H, d, J 7.0 Hz, H-6). On the other hand, the 13C NMR spectrum displayed the signals at 174.7 and 180.7 ppm for carbonyl groups.
Electrochemical studies
The reduction pathway is a function of parameters that may be directly associated with the acceptor molecule, such as the nature of the cleaving bond, the properties of substituents, and the redox potential of the leaving group, or externally driven by factors such as temperature, solvent, and the electrode potential (for heterogeneous reductions).4343 Lehmann, M. W.; Evans, D. H.; J. Electroanal. Chem. 2001, 500, 12.
The electrochemical reduction of quinones had been extensively studied and was shown to depend strongly on the reaction media.1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun. 2008, 2612.,1919 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.,4444 Meneses, A. B.; Antonello, S.; Arevalo, M. C.; Maran, F.; Electrochim. Acta 2005, 50, 1207.
In typical measurements, CVs were recorded in aprotic medium (DMF + TBAPF6, 0.1 mol L-1), at a scan rate of 100 mV s-1. This enabled the determination of the electrochemical reduction (from +0.5 up to -3.0 V) and oxidation (from -0.5 up to +1.5 V) behaviors of each compound. Cathodic and anodic peak potentials for each compound are listed in Table 1.
Electrochemical parameters for CV (DMF + TBAPF6, 0.1 mol L-1), GCE, ν = 0.100 V s-1 vs. Ag|AgCl|Cl−. QS represents the thionaphthoquinones
The electrochemical study of the precursor, nor-beta-lapachone (1), was already reported3535 de Abreu, F. C.; Ferraz, P. A. L.; Goulart, M. O. F.; J. Braz. Chem. Soc. 2002, 13, 19. in slightly different conditions (DMF + TBAP, 0.1 mol L-1). In the present case, its CV, in DMF + TBAPF6, shows the presence of two sequential mono-electronic, diffusional-controlled (EpIc α ν1/2), quasi-reversible waves (EpIc = -0.646 V; EpIIc = -1.180 V). They correspond, as already known, to the generation of the semiquinone (Q•), followed, in the second step, to the dianion (Q2-) formation (Figure 3a). DPV experiments were also performed, showing two peaks (Figure 3b).
(a) Cyclic voltammetry (CV) of nor-beta-lapachone (1) (1 mmol L-1) in DMF + TBAPF6 (0.1 mol L-1), GCE, ν = 100 mV s–1. Several inversion potentials in the CV of 1; (b) differential pulse voltammetry (DPV) curve of 1 (1 mmol L-1) at the same conditions, during reduction process. Potential range from 0 to −2.0 V, with a pulse amplitude of 50 mV and a sample pulse width of 70 ms, ν = 10 mV s-1. Arrow indicates initial scan direction.
The CV of the thionaphthoquinones, represented by compound 2, displayed peaks in the cathodic (Figure 4a) and anodic regions (Figure 4b). Figure 4c is a combination of both regions and Figure 4d shows the scan rate effect on the several reductions and oxidation waves, proving the diffusional and quasi-reversible nature of the electrochemical process. The CVs of the other thionaphthoquinones (2-8, 11) are presented in the SI section (Figure S5) and are similar.
Cyclic voltammetry (CV) of 2 (1 mmol L-1) in DMF + TBAPF6 (0.1 mol L-1), GCE, ν = 100 mV s–1. (a) Cathodic and (b) anodic direction. Several inversion potentials in the CV of 2; inserts: successive CVs; (c) cathodic direction (black line) and anodic direction (red dashed line); (d) different scan rates: 10, 20, 35, 50, 75, 100, 200, 300, 400 and 500 mV s-1.
For the sake of simplicity, in the electrodic mechanism rationalization, the compounds are represented by [Q]-spH-SR, being spH a spacer, in this case, a dihydrofuranic ring, with a methynic carbon in the junction with the thioaromatic or thioaliphatic or thioacetate group; SR is an aromatic sulfide in the majority of cases and non-aromatic in three compounds (10-12). It is important to emphasize that both redox moieties (Q and SR) are not resonance-conjugated (the reason for writing spH, between them), and behave as independent redox functions.
As expected, the overall CV profiles, in the first part of the cathodic region, for the thionaphthoquinones, are similar to the one reported for compound 1 (Figure 2) and other quinones (Figure S17, SI section).1919 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.,2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem 2016, 3, 2252.,4343 Lehmann, M. W.; Evans, D. H.; J. Electroanal. Chem. 2001, 500, 12. Two couples of cathodic and anodic peaks are monoelectronic, diffusional (EpIc α ν1/2) and have quasi-reversible nature (Ic/Ia). As well-known, the first pair is related to the anion-radical (semiquinone) formation, and the second pair of peaks is broader and less-defined (IIc/IIa), as observed before,4343 Lehmann, M. W.; Evans, D. H.; J. Electroanal. Chem. 2001, 500, 12. due to possible chemical reactions of the ortho-semiquinones. A number of deviations from ideal behavior have been identified, some readily interpretable and others of completely unknown origin.4343 Lehmann, M. W.; Evans, D. H.; J. Electroanal. Chem. 2001, 500, 12.
The values of EpIc varied from -0.556 (9, pentafluorobenzene derivative) up to -0.655 V, for the cyclohexyl one (11) (Table 1).
The CVs of compounds 9 and 12, which substituents are a pentafluorobenzene and a non-aromatic thioacetate, respectively (Figure 5), display a different profile (Figures 5a and 5b). Wave IIc for compound 9 is broader than for compounds 1-8, with an additional shoulder, which always appears in the presence of halogenated compounds. For compound 12, several other reduction waves appear, being the first wave more prominent than the other ones. The complexity of the CV profile may indicate bond cleavage, generating other electroactive systems2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem 2016, 3, 2252. and lack of stability. In the oxidation region, for compound 12, the behavior is similar to the other thionaphthoquinones, while for 9, the anodic wave is smaller than in all other cases, suggesting a mono-electronic oxidation leading to a cation-radical, which suffers dimerization, due to the electron-withdrawing characteristics of the perfluorinated ring.
Cyclic voltammetry (CV) of 9 (left) and 12 (right) (1 mmol L-1) in DMF + TBAPF6 (0.1 mol L-1), GCE, ν = 100 mV s–1. (a) Cathodic and (b) anodic direction-several inversion potentials in the CVs of 9 and 12; inserts: successive CVs; (c) cathodic direction (black line) and anodic direction (red dashed line).
For the complete set of thionaphthoquinones, a third irreversible reduction wave appears, with more negative potentials (Figure S5, SI section) (Table 1) and more intense current, related to the capture of 2 electrons. These peaks are irreversible at all scan rates investigated and broaden as n increases. Previous works by Maran and co-workers4444 Meneses, A. B.; Antonello, S.; Arevalo, M. C.; Maran, F.; Electrochim. Acta 2005, 50, 1207.,4545 Meneses, A. B.; Antonello, S.; Arvalo, M. C.; Gonzalez, C. C.; Sharma, J.; Wallette, A. N.; Workentin, M. S.; Maran, F.; Chem. - Eur. J. 2007, 13, 7983. reported similar behavior for diphenylmethyl para-methoxyphenyl sulfide, with occurrence of C-S bond cleavage and self-protonation mechanisms.4646 Viertler, H.; Gruber, J.; Pardini, V. L. In Organic Electrochemistry; Lund, H.; Hammerich, O., eds.; Marcel Dekker Inc.: New York, 2001, p. 621 (and included references). In our case, the last mechanism does not occur, due to the lack of dissociable acidic functions, however, protonation is possible in the generated carbanion function, due to residual water or proton capture from the supporting electrolyte, by Hoffman elimination.
A possible mechanism is represented by equations 2-4. The thiophenolate ion (-SR) can be oxidized at IIIa, a small shoulder present in all the CVs, which only appear at potential reversal close to IIIc (equations 5-6).
As the S-phenyl moiety is not resonance-conjugated with the reducible naphthoquinone moiety, there is only a slight change in the reduction potentials for the majority of compounds. The difference of 100 mV was observed between compound 9 (-C6F5), the most easily reduced and the aliphatic S derivative (11), reduced at more negative potentials.
The electron withdrawing substituents are Cl (σCl = 0.22), F (σF = 0.06), while electron donating ones are para-methyl (σCH3 = -0.17), meta-methyl (σCH3 = -0.06) and methoxy (σOCH3 = -0.28), among others.
The electrophilicity order for the present compounds, represented by EpIc values, is: 9 (-0.556 V) ca. 12 (-0.562 V) < 8 (-0.588 V) ca. 7 (-0.590 V) ca. 2 (-0.594 V) ca. 4 (-0.598 V) ca. 6 (-0.604 V) < 3 (-0.614 V) ca. 5 (-0.620 V) ca. 10 (-0.624 V) < 11 (-0.655 V).
In the anodic region of all the quinones (Figures 4 and 5, Figure S5, SI section), a sharp, irreversible peak (IVa) appears. The currents of these anodic peaks vary with the structure.
In the case of compound 2, the intensity of the anodic peak (IVa) is, at least, twice higher, in relation to the first reduction peak (Ic) (Figure 4c). In the reversed cycle, two new reduction waves (IVc and Vc) are observed close to 0 V; in the successive scan, it is observed that the reduced product formed at IVc can be re-oxidized (IVa') (Figure 4b, insert).
The quite high oxidation potential of the 3-phenylthio-dihydronaphtho[1,2-b]furan-4,5-diones attests that the sulfur atom is the first part of the molecule to be oxidized to mono- and di-positive ions, which interact further with anions or with the starting materials.4646 Viertler, H.; Gruber, J.; Pardini, V. L. In Organic Electrochemistry; Lund, H.; Hammerich, O., eds.; Marcel Dekker Inc.: New York, 2001, p. 621 (and included references).
47 Dakova, B.; Lambews, I.; Evers, M.; Electrochim. Acta
1994, 39, 2363.-4848 Baciocchi, E.; Rol, C.; Scamosci, E.; Sebastiani, G. V.; J. Org. Chem.
1991, 56, 5498. The phenyl moiety is involved in charge delocalization only if it is substituted with strong electron donating groups,4747 Dakova, B.; Lambews, I.; Evers, M.; Electrochim. Acta
1994, 39, 2363.,4949 France, R. R.; Rees, N. V.; Wadhawan, J. D.; Fairbanks, A. J.; Compton, R. G.; Org. Biomol. Chem. 2004, 2, 2188. as is the case of compound 5, in which, the anodic peak IVa is followed by a more positive one (Va) (Figure S16, SI section).
The products of the electrochemical oxidation of alkylarylsulfides (RSAr) are strongly dependent upon the structure of the alkyl/aryl group linked to it, as well as by the nature of the electrolysis medium and the water content of the solvent.4848 Baciocchi, E.; Rol, C.; Scamosci, E.; Sebastiani, G. V.; J. Org. Chem. 1991, 56, 5498.,4949 France, R. R.; Rees, N. V.; Wadhawan, J. D.; Fairbanks, A. J.; Compton, R. G.; Org. Biomol. Chem. 2004, 2, 2188.
The first mono-electronic capture leads to the formation of the corresponding S radical-cation, for which four reaction paths are generally available: deprotonation; nucleophilic attack at sulfur; irreversible C-S bond cleavage and nucleophilic attack at the aromatic ring. The relative weight of each of these reaction pathways is governed by the facts already mentioned.4747 Dakova, B.; Lambews, I.; Evers, M.; Electrochim. Acta
1994, 39, 2363.
48 Baciocchi, E.; Rol, C.; Scamosci, E.; Sebastiani, G. V.; J. Org. Chem.
1991, 56, 5498.-4949 France, R. R.; Rees, N. V.; Wadhawan, J. D.; Fairbanks, A. J.; Compton, R. G.; Org. Biomol. Chem. 2004, 2, 2188. It shows a simple CV for a very complex mechanism. It will be further explored, but in summary, it can be represented by the usual pathways4949 France, R. R.; Rees, N. V.; Wadhawan, J. D.; Fairbanks, A. J.; Compton, R. G.; Org. Biomol. Chem. 2004, 2, 2188.
50 Sawyer, D. T.; Velentine, J. S.; Acc. Chem. Res.
1981, 14, 393.-5151 Vasudevan, D.; Wendt, H.; J. Electroanal. Chem. 1995, 192, 69. (Figure 6), as discussed in the previous paragraph.
The electrochemical oxidation profile, based on the values of EpIVa (Table 1, column 9), is related to effects of the substituents, with oxidation facilitated by electron donating ones.
The order of oxidation facility is: 11 (1.133 V) > 5 (1.138 V) > 3 (1.187 V) > 7 (1.200 V) ca. 4 (1.204 V) > 10 (1.211 V) > 12 (1.250 V) > 6 (1.299 V) ca. 2 (1.300 V) > 8 (1.340 V) > 9 (1.350 V).
Reactivity with oxygen
The investigation of the interaction of oxygen with quinones and respective radical anions can be performed through cyclic voltammetry, in aprotic media.1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun.
2008, 2612.
19 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.-2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252.,3636 Goulart, M. O. F.; Ossowski, T.; Pipka, P.; Liwo, A.; Bioelectrochemistry
2003, 59, 85.
37 Goulart, M. O. F.; Lima, N. M. F.; Santana, A. E. G.; Ferraz, P. A. L.; Cavalcanti, J. C. M.; Liwo, A.; Falkowsky, P.; Ossowsky, T.; J. Electroanal. Chem.
2004, 566, 25.-3838 Bard, A. J.; Faulkner, L. R.; Electrochemical Methods: Fundamentals and Applications; Wiley: New York, 2001. It mimics one of the most important mechanisms of molecular action of quinones, particularly, the generation of ROS after reduction and electron transfer to oxygen.1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun.
2008, 2612.
19 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.-2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem
2016, 3, 2252.,3636 Goulart, M. O. F.; Ossowski, T.; Pipka, P.; Liwo, A.; Bioelectrochemistry
2003, 59, 85.,3737 Goulart, M. O. F.; Lima, N. M. F.; Santana, A. E. G.; Ferraz, P. A. L.; Cavalcanti, J. C. M.; Liwo, A.; Falkowsky, P.; Ossowsky, T.; J. Electroanal. Chem.
2004, 566, 25.
Non-aqueous polar solvents are chosen as better models of a membrane environment, in which peroxidation processes take place. Both the superoxide anion radical and its conjugated acid, the hydroperoxyl radical, are long-lived species in aprotic media5050 Sawyer, D. T.; Velentine, J. S.; Acc. Chem. Res. 1981, 14, 393.,5151 Vasudevan, D.; Wendt, H.; J. Electroanal. Chem. 1995, 192, 69. and unstable in water and other protic solvents, owing to their fast disproportionation.5252 Antunes, F.; Salvador, A.; Marinho, H. S.; Alves, R.; Pinto, R. E.; Free Radical Biol. Med. 1996, 21, 917.,5353 Song, Y.; Buettner, G. R.; Free Radical Biol. Med. 2010, 49, 919. In DMF or DMSO, O2•- is a long-lived species and stable even at low-scan-rate voltammetry.
The electrochemical behavior of compound 2, in presence of oxygen is displayed in Figure 7. All the thionaphthoquinones have a similar feature, with different catalytic constants. Additional graphs are presented in the SI section (Figure S18).
CV for 2, in DMF + TBAP (0.1 mol L-1), GCE, ν = 50 mV s-1, cathodic direction. CV in absence (red line) and in presence (black lines) of different concentrations of oxygen. Potential range: from 0.5 V up to −0.7 V. Insert: Linear portion of IpR/IpO in function of oxygen concentration.
With the successive bubbling of oxygen, the voltammetric profile relative to the first monoelectronic reduction wave changes. A positive slight shift of the cathodic potential and an increase of the reduction current occur as the oxygen concentration increases, with a concomitant disappearance of the anodic peak. This finding evidences reaction of the semiquinone with oxygen, in a homogeneous catalytic electron transfer (Figure 7, equations 2 and 7).
Data obtained from the addition of different concentrations of oxygen (Figure 7 and insert) allow to determine the apparent association constants between the electrogenerated semiquinones and O2 from the graph Ip1c/IpO1 vs. kap[O2]RT/nFv, considering that the maximum solubility of oxygen in DMF is 1.85 mmol L-1 at 25 ºC.
The kap obtained can be related to the capacity of generation of superoxide anion radical. The respective constants of interaction with oxygen, together with the compound number, are listed in increasing order of reactivity: 3 (0.06 s-1) < 4 (0.10 s-1) ca. 2 (0.11 s-1) ca. 8 (0.12 s-1) < 6 (0.18 s-1) < 5 (0.32 s-1) < 7 (0.69 s-1).
Comparing with other quinones,3434 Ferreira, F. R.; Ferreira, S. B.; Araújo, A. J.; Marinho Filho, J. D. B.; Pessoa, C.; Moraes, M. O.; Costa-Lotufo, L. V.; Montenegro, R. C.; Silva, F. C.; Ferreira, V. F.; da Costa, J. G.; de Abreu, F. C.; Goulart, M. O. F.; Electrochim. Acta 2013, 110, 634. using the same method, the present values, except for 7 (4-Cl derivative), are much lower than the ones obtained for arylated and dihydrofuran naphthoquinones, which showed values in the range of 0.45 up to 1.03 s-1,3434 Ferreira, F. R.; Ferreira, S. B.; Araújo, A. J.; Marinho Filho, J. D. B.; Pessoa, C.; Moraes, M. O.; Costa-Lotufo, L. V.; Montenegro, R. C.; Silva, F. C.; Ferreira, V. F.; da Costa, J. G.; de Abreu, F. C.; Goulart, M. O. F.; Electrochim. Acta 2013, 110, 634. and correlated well with ROS release in cancer cells. This may be a hint about the mechanism, not entirely controlled by oxidative stress, like reported before for the pterocarpanequinone LQB 118 ((7aS*,12aS*)-7,7a-dihydro-5H-benzo[g]benzofuro[3,2-c]chromene-5,13(12aH)-dione),2020 Silva, T. L.; Ferreira, F. R.; Vasconcelos, C. C.; da Silva, R. C.; Lima, D. J. P.; Costa, P. R. R.; Netto, C. D.; Goulart, M. O. F.; ChemElectroChem 2016, 3, 2252. which presented kinetic data similar to the present ones (0.17 s-1).
Biological results
Eleven thionaphthoquinones were assayed against four human tumor cell lines, SF-295 (human glioblastoma), HCT-116 (human colon carcinoma), PC3 (prostate) and K562 (leukemia) and towards PBMC (peripherical blood mononuclear cell), using effective concentration that leads to the death of 50% of the cells (IC50).
The compounds were classified according to their activity as highly active (IC50 < 2 µM, moderately active (2 µM < IC50 < 10 µM) or inactive (IC50 > 10 µM).5454 Perez-Sacau, E.; Diaz-Peñate, R. G.; Estevez-Baun, A.; Ravelo, A. G.; Garcia-Castellano, J. M.; Pardo, L.; Campillo, M.; J. Med. Chem. 2007, 50, 696.
Table 2 lists the values of the cytotoxic activity (IC50) in µg mL-1 (mM) of nor-beta-lapachone (1), already described,5555 da Silva Jr., E. N.; de Deus, C. F.; Cavalcanti, B. C.; Pessoa, C.; Costa-Lotufo, L. V.; Montenegro, R. C.; Moraes, M. O.; Pinto, M. C. F. R.; Simone, C. A.; Ferreira, V. F.; Goulart, M. O. F.; Andrade, C. K. Z.; Pinto, A. V.; J. Med. Chem. 2010, 53, 504. doxorubicin, along with new data from the 11 thionaphthoquinones, with the respective 95% confidence interval. Figure 8 allows a better comparative analysis.
Cytotoxic activity (IC50) of 1-12 and doxorubicin against human tumor cell lines and towards PBMC
Graph showing IC50 values (μM) of the thionaphthoquinones ([Q]-spH-SR) (1-12), for the 4 cancer lines studied, in comparison with doxorubicin (13).
Analysis of Table 2 and Figure 8, with the results of IC50, allows to evidence that all the hybrid compounds have shown cytotoxicity against various cell lines with a similar profile. In general, the majority of the thionaphthoquinones had shown cytotoxic activity lower than 5 mM, except for compound 11, with higher cytotoxicity against normal cells than for cancer cell lines.
K562 was the most sensitive cell line, with several values of IC50 lower than 2 μM, reaching the lowest value of 0.9 μM for compound 2, while SF-295 was the most resistant, allowing to discriminate compound 8 as the less active compound in the series. The cytotoxicity of the compounds toward SF295 was lower than 5 μM, except for compounds 8 and 11.
When evaluating the prostate cancer cell line (PC3), IC50 values < 5 μM were obtained for the majority of compounds, except for compounds 11 and 12, being compound 6, highly active. Toward the colon carcinoma HCT-116, a similar profile is observed, with IC50 values close to 2 μM for 2, 3, 5, 6, 8 and 10.
Table 3 lists the selectivity index (SI) (SI = IC50 PBMC/IC50 cancer line) for all the quinones, concerning each cancer line. SI allows the evaluation of the selectivity of each compound toward cancer cells and could indicate the potential for further pre-clinical studies.5454 Perez-Sacau, E.; Diaz-Peñate, R. G.; Estevez-Baun, A.; Ravelo, A. G.; Garcia-Castellano, J. M.; Pardo, L.; Campillo, M.; J. Med. Chem. 2007, 50, 696.
Figure 9 allows a better visualization, for comparison purposes, showing the threshold of 2, as the limit to be considered significant in terms of selectivity index.
Graph showing selectivity indexes, for the 4 cancer cell lines studied for the 12 thionaphthoquinones, using 2 as a referenced selectivity index.
Suffness and Pezzuto5858 Suffness, M.; Pezzuto, J. M. In Methods in Plant Biochemistry: Assays for Bioactivity, vol. 6; Hostettmann, K., ed.; Academic Press: London, 1990, p. 71. considered SI significant for values greater than or equal to 2.0, i.e., that value means that the compound exhibits twice more activity toward the neoplastic cell line than in normal cells. SI values lower than 2.0 indicate low selectivity. Only three quinones had SI values higher than 2, being: 5, in which the SI values are 2.3 and 3.14 against K562 and HCT-116, respectively; compound 2 shows an SI of 2.11 against K562 and 2.96 against HCT-116 and finally, 6, with SI of 2.34 toward HCT-116.
These differences in IC50 values are minor, yet noticeable. Still, there is no significant “selective cytotoxicity” against one particular cell line, which is somewhat disappointing yet not entirely, unexpected.
Compound 2 increases intracellular ROS levels
Generation of intracellular ROS is considered one of the key mediators of quinone's anticancer effects. Therefore, the redox status of treated HCT-116 cells was monitored using the oxidation-sensitive fluorescent dye CM-H2DCFDA after 30 min, 1 and 3 h of incubation. Compound 2 induced an increase in intracellular ROS levels in both concentrations tested. In the lower concentration (2.4 µM), an increase in DCF fluorescence was detected in a time-dependent manner, with the fluorescence being more intense at 3 h. In the higher concentration (4.8 µM), the DCF fluorescence was similar among the periods of time tested, being more intense in the first 30 min (Figure 10). As expected, menadione and hydrogen peroxide, used as positive controls, increased ROS generation in treated cells, with a peak of production at 1 h.
Effect of compound 2 (C2) on ROS production in HCT-116 cells, determined by flow cytometry using CM-H2DCFDA, after 30 min, 1 and 3 h incubation. Menadione (MEN, 20 μM) and hydrogen peroxide (H2O2, 100 μM) were used as positive controls and C- is the negative control (buffer alone). A total of 5,000 events were analyzed per sample. Data are expressed as mean ± SEM from two independent experiments.
As several other clinically used quinones, compound 2 was able to increase intracellular ROS levels over a critical threshold. The high ROS content in cancer cells, due to their accelerated metabolism, renders them more susceptible to oxidative stress-induced cell death, and can be exploited for selective cancer therapy.
In the class studied, the insertion of a sulfur atom increased the polarizability, conferring an increase in lipophilicity, which suggests a change in the profile related to this aspect in relation to nor-beta-lapachone (1).
The activity of biologically active compounds is related to their physicochemical properties, since they must cross membrane barriers and reach their receptor site (s) in order to perform their action, thus triggering the biological response. In electrochemistry, the main thermodynamic parameter of correlation with the biological activity of a specific group is the potential of the first reduction wave (EpIc).1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun. 2008, 2612. A graph between IC50 and EpIc of the quinones was built (Figure 11). Although the data available to date is limited, hampering a definite structure-reactivity relationship, there is some support for this notion.
Relationship between EpIc (potential of the first reduction peak), in V, and IC50 (µM), in 4 cancer cell lines and PBMC, with colors shown inside the figure. Numbers are related to the compounds. Compounds 8, 11 and 12 were less active. Compound 1 (nor-beta-lapachone), the standard, is not toxic toward PBMC.
Despite the absence of a linear correlation in the graph, there is a trend and an optimal range for the reduction potential. All the compounds, in aprotic medium, with values of first wave reduction potential (EpIc), larger (less negative) than -0.65 V, were active (IC50 lower than 5 µM). Compounds 8 and 11, referring to the SF-295 lineage, were outside this biological activity region. This trend was evidenced in some articles,1818 Hillard, E. A.; de Abreu, F. C.; Ferreira, D. C. M.; Jaouen, G.; Goulart, M. O. F.; Amatore, C.; Chem. Commun. 2008, 2612.,1919 de Paiva, Y. G.; Ferreira, F. R.; Silva, T. L.; Labbé, E.; Buriez, O.; Amatore, C.; Goulart, M. O. F.; Curr. Top. Med. Chem. 2015, 15, 136.,5656 da Silva Jr., E. N.; de Souza, M. C. B. V.; Pinto, A. V.; Pinto, M. C. F. R.; Goulart, M. O. F.; Barros, F. W. A.; Pessoa, C.; Costa-Lotufo, L. V.; Montenegro, R. C.; de Moraes, M. O.; Ferreira, V. F.; Bioorg. Med. Chem. 2007, 15, 7035.,5757 Cavalcanti, B. C.; Barros, F. W. A.; Cabral, I. O.; Ferreira, J. R. O.; Magalhães, H. I. F.; Júnior, H. V. N.; da Silva Júnior, E. N.; de Abreu, F. C.; Costa, C. O.; Goulart, M. O. F.; Moraes, M. O.; Pessoa, C.; Chem. Res. Toxicol. 2011, 24, 1560.,5959 Koyama, J.; Morita, I.; Yamori, T.; Molecules 2010, 15, 6559. despite the complexity of cell chemistry, redox medicine and cancer disease.
Conclusions
The electrochemical results have confirmed the presence of two distinct and independent redox couples in this series: the quinone and the thio component. In the biological perspective, agents containing chalcogens and quinones can be used to attack entities with a disturbed redox balance, while protecting healthy cells, working as antioxidants and leading to highly selective redox therapeutic agents.
The work presented here should be seen as providing the impetus for follow-on investigations, including synthetic chemistry and cell biology. These molecules are not only intriguing from a purely synthetic point of view. They also provide several interesting leads for possible drug development, which are not limited to cancer research, but also appear to include lesser exposed, yet equally important areas of pharmaceutical research, such as antimalarial and anti-fungal therapies, being a fertile ground for various multidisciplinary follow-on projects.6060 Mecklenburg, S.; Shaaban, S.; Ba, L. A.; Burkholz, T.; Schneider, T.; Diesel, B.; Kiemer, A. K.; Röseler, A.; Becker, K.; Reichrath, J.; Stark, A.; Tilgen, W.; Abbas, M.; Wessjohann, L. A.; Sasse, F.; Jacob, C.; Org. Biomol. Chem. 2009, 7, 4753.
These studies are, of course, only an entry point for considerably wider and more in-depth investigations, which ultimately may also consider mechanistic aspects and biochemical mode(s) of action.6060 Mecklenburg, S.; Shaaban, S.; Ba, L. A.; Burkholz, T.; Schneider, T.; Diesel, B.; Kiemer, A. K.; Röseler, A.; Becker, K.; Reichrath, J.; Stark, A.; Tilgen, W.; Abbas, M.; Wessjohann, L. A.; Sasse, F.; Jacob, C.; Org. Biomol. Chem. 2009, 7, 4753.
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Supplementary InformationSupplementary data (NMR, FTIR, HRMS, cyclic voltamograms) are available free of charge at http://jbcs.sbq.org.br as a PDF file.
Acknowledgments
The authors wish to thank the Brazilian financial agencies: CNPq, CAPES, FAPEAL, FAPESP and INCT in Bioanalytics (FAPESP No. 2014/50867-3 and CNPq No. 465389/2014-7, 458114/2014-6). M. F. C. C., V. F. F. and F. C. S. thank to FAPERJ for the grants and fellowships.
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Publication Dates
-
Publication in this collection
Mar 2019
History
-
Received
11 Aug 2018 -
Accepted
18 Dec 2018