Removal of the cortical projections alters expression of NOS in the different cell types of the superficial layers of the superior colliculus in rats

Removal of the cortical projections alters expression of NOS in the different cell types of the superficial layers of the superior colliculus in rats

FRANK TENÓRIO¹, ARTHUR GIRALDI-GUIMARÃES² and ROSALIA MENDEZ-OTERO²

¹Departamento de Farmacologia e Psicobiologia, Instituto de Biologia Roberto Alcântara Gomes, UERJ 20551-030 Rio de Janeiro, Brazil

²Instituto de Biofísica Carlos Chagas Filho, UFRJ, 21941-590 Rio de Janeiro, Brazil

Manuscript received on September 13, 2002; accepted for publication on September 20, 2002;

contributed by ROSALIA MENDEZ-OTERO^*

ABSTRACT

Nitric oxide has several biological roles and nitric oxide synthase (NOS) is expressed in the nervous system, and co-localizes with NADPH-diaphorase. The superficial layers of the superior colliculus (SC), which receive retinal and cortical inputs, present NADPH-d staining in a sub-population of neurons that include all cell types. We have previously shown, by NADPH-diaphorase, that eye enucleation alters the intracellular distribution of NOS. Here, we studied the effect of cortical ablation on NOS expression by neurons in collicular superficial layers. Our results show that cortical ablation alters the proportion of different NOS-positive cell types, but not the intracellular distribution of the enzyme.

Key words: superior colliculus, nitric oxide, visual cortex, NADPH-diaphorase.

1. INTRODUCTION

Nitric oxide (NO) is a gas that plays several roles in the central nervous system. Nitric oxide synthase (NOS) is highly expressed in the nervous system and NADPH-diaphorase staining is highly correlated with NOS activity (Bredt et al. 1991, Dawson et al. 1991, Hope et al. 1991, Matsumoto et al. 1993). Despite of being a constitutive enzyme, neuronal NOS activity and its sub cellular distribution appears to be actively regulated at multiple levels (Vincent 1995).

Results form different laboratories suggest that retinal projections and/or its neural activity might have a role in the expression of this enzyme in the visual system. Light deprivation enhances NOS activity in the lateral geniculate nucleus (LGN) of mice and rabbits (Ientile et al. 1996) and enhances NADPH-d staining in the LGN of cats (Gunluk et al. 1994). Monocular enucleation also alters NOS distribution in the monkey visual cortex (Aoki et al. 1993).

In previous studies we have shown in rats that the removal of the retinocollicular projections by enucleation lead to an alteration of intracellular distribution of NADPH-d staining in NOS positive neurons at the superior colliculus superficial layers (Tenório et al. 1998). The superficial layers of the SC also receive an input from cortical pyramidal cells of ipsilateral visual cortex (Bolz et al. 1991). To investigate whether cortical projections have some influence in NOS expression in the superior colliculus superficial layers, we studied the pattern of NADPH-d staining in neurons from these layers in animals in which the normal pattern of cortico-collicular projections was disturbed by removal of the visual cortex.

2. MATERIALS AND METHODS

Lister rats from our breeding colony were used. All chemicals were purchased from Sigma Chemical Co. (St. Louis, MO). Newborn animals (P0) were anaesthetized by hypothermia to be submitted to visual cortex removal surgery (n=5). An incision was made at the top of the head. The skull was opened and the region corresponding to the left occipital cortex was aspirated with a pipette tip attached to a pump. The left SC was the experimental one and the right SC of the same animal was used as the control. The animals were sutured and returned to their mother after recovering from the surgery. In addition, control animals (n=9) were used for comparison. At ages between P30 and P60, animals were anaesthetized with sodium pentobarbital (50mg/kg) and intracardially perfused with 0.9% saline solution followed by 4% paraformaldehyde (PF) in 100mM phosphate buffer (pH 7.4). After dissection, the brains were immersed in 100mM phosphate buffer containing 20% sucrose, overnight at 4°C and then sectioned at 60µm on a cryostat at -20°C in the coronal or parasagital planes. Sections containing the SC were collected in 50 mM Tris buffer (pH 7.4), washed twice in this buffer and submitted to NADPH-d histochemistry technique. After 1h at 37°C under constant shaking, reaction was interrupted, sections were washed twice in Tris buffer and mounted in gelatinized slides. The slides with sections were dehydrated and coversliped. Photomicrographs were made using a Zeiss Axioplan microscope. Cell counts were performed under a 40X objective. Four fields in sections of 4 animals of each group were used. Piriform and wide field vertical cells were included in the same group (ganglion cells type I and II). The differences between the control group and ablated animals were analyzed using a c² -test for k independent samples; critical values of p<0.001 were used for analyses.

3. RESULTS AND DISCUSSION

Previously we have shown that eye enucleation do not interfere with the temporal sequence of NOS expression in the rat SC superficial layers, although it alters the intracellular distribution of this enzyme by these cells (Tenório et al. 1998). After removal of the retinal projections the SC superficial layers still receive projections from the ipsilateral visual cortex. The removal of these collicular afferents could origin different changes, from those seem in enucleated animals, in the pattern of NOS expression by neurons of the SC superficial layers.

Our results showed no significant difference in the general pattern of NADPH-d staining between the deafferented and the afferented SC (Fig.1). The staining pattern in these animals was also very similar to the pattern described in control animals SC (non operated) (data not shown). In normal animals we have demonstrated that (Tenório et al. 1996), a sub-population of all the different cell types described with the Golgi staining in the rat SC (Langer and Lund 1974) expressed NOS (Tenório et al. 1996). The same cell types were also stained in both deafferented and afferented SC of the ablated animals. However, cell counts in normal and ablated animals revealed that the proportion of NADPH-d stained cells was altered in relation to the different cell types (Table I). Narrow field vertical cells showed a marked decrease (54.6%) in their proportion in relation to other cells types when comparing control and ablated animals. On the other hand, stellate cells are the ones that shown the more pronounced increase in proportion to other cell types tripling their number. Type I and II ganglion cells and horizontal cells (deep and surface) double their number in ablated animals in comparison to control animals (232.18%; 171.43%; 200.00% respectively). Marginal, type III ganglion cells and non-classified cells showed no significant difference in their proportion in relation to the control group.

The difference in the results between the ablation of retinal afferents and cortical inputs shows that these projections might have different influences on the expression of NOS by SC cells and it is possible to suggest that this difference might be correlated with different functional roles of these afferents. In fact, it has been shown that the ablation of visual cortex facilitated induction of LTP in the SC superficial layers, indicating that the corticocollicular projection could have a modulatory function, blocking the enhancing of the synaptic transmission probably by activation of GABAergic neurons (Hirai and Okada 1993). In various mammalian species, the SC superficial layers contain the highest amount of GABA, and the main source of this neurotransmitter in these layers is the large number of GABAergic interneurons (Mize et al. 1982, Okada 1974). We have previously shown that 20% of all NOS-positive cells in the rat SC superficial layers also expressed GABA (Soares-Mota et al. 2001). It is difficult at this time to establish any correlation between these data, and work in our lab is in progress to determine which collicular cell types are double-stained by NOS and GABA, and what are the effects of the ablation of the visual cortical and retinal afferents in this co-expression. However, results from other laboratories allow us to make some inferences. In cats, the GABAergic neurons have the cortical projections as a very important synaptic source (Mize 1988). These cells were described as horizontal I, granule I and granule II and, by the morphological similarities, they probably correspond to the superficial horizontal and the ganglion cells, respectively, described in rats and found in our work. Our results showed that cortical ablation induces an increase in the proportion of the NADPH-d stained cells of these groups, in relation to control animals. Then it is possible to suggest that the absence of cortical projections, which is an important source of synaptic input to these cells, directly induces an increase in the NOS expression by them. However, there are a few points against this conclusion. First, the afferent inputs to the deep horizontal cells, the proportion of which also increases, are unknown. Second, although there are a large number of reports involving the study of the sensory processing in the SC, the complete synaptic circuitry underlying SC function remains not completely understood. So, it is also possible that the effects we described after removal of the cortical projection are due to indirect effects of the absence of these projections onto SC cells.

RESUMO

O óxido nítrico apresenta diversos papéis biológicos e a óxido nítrico sintase (ONS) é expressa no sistema nervoso, co-localizando-se com a NADPH-diaforase. As camadas superficiais do colículo superior (CS), as quais recebem aferências retinianas e corticais, possuem marcação para NADPH-diaforase numa sub-população de neurônios que inclui todos os tipos celulares. Previamente demostramos, por NADPH-diaforase, que a enucleação ocular altera a distribuição intracelular da ONS. Neste trabalho estudamos o efeito da ablação cortical na expressão da ONS por neurônios das camadas superficiais coliculares. Nossos resultados demonstraram que a ablação cortical altera as proporções dos diferentes tipos celulares ONS positivos mas não a distribuição intracelular da ONS.

Palavras-chave: colículo superior, óxido nítrico, córtex visual, NADPH-diaforase.

Correspondence to: Frank Tenório

E-mail: [e-mail]

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*

Member of Academia Brasileira de Ciências

Publication Dates

• Publication in this collection
  24 Jan 2003
• Date of issue
  Dec 2002

History

• Received
  13 Sept 2002
• Accepted
  20 Sept 2002