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Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria

Avaliação de marcadores fenotípicos associados à patogenicidade no gênero Listeria

Abstracts

A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produce keratoconjunctivitis in guinea pigs. L. monocytogenes cultures presented 51.8% and 88.8% positivity rates for Congo red adsorption and lecithinase production, respectively, whereas 80.8% and 100% for L. innocua cultures were negative for the two test, respectively

Listeria monocytogenes; Hemolysin; Lecithinase; Congo red adsorption; Keratoconjunctivitis


Com propósito de avaliar a produção de lecitinase e a capacidade de adsorção do corante vermelho Congo como marcadores de patogenicidade, foram estudadas 130 amostras de Listeria. Estas amostras foram identificadas segundo a produção de ácido a partir de açúcares aliada ao teste CAMP, correlacionando-se estes dados à capacidade de produção de ceratoconjuntivite em cobaio. As culturas de L. monocytogenes apresentaram taxas de positividade para a adsorção do corante e produção de lecitinase de 51,8 e 88,8%, respectivamente, enquanto 80,8% e 100% das culturas de L. innocua foram negativas para os referidos testes.


MICROBIOLOGY

Evaluation of phenotypic markers associated with pathogenicity in the genus Listeria

Avaliação de marcadores fenotípicos associados à patogenicidade no gênero Listeria

Zilma das Graças Nunes; Ernesto HoferI

IDepartment of Bacteriology, Oswaldo Cruz Institute, FIOCRUZ, Av. Brasil, 4365; Manguinhos; 21045-900 Rio de Janeiro, RJ, Brasil

SUMMARY

A total of 130 Listeria strains were tested in order to evaluate lecithinase production and capacity for Congo red adsorption as markers of pathogenicity. The strains were identified according to acid production from sugars and by the CAMP test and the data were correlated with the ability to produce keratoconjunctivitis in guinea pigs.

L. monocytogenes cultures presented 51.8% and 88.8% positivity rates for Congo red adsorption and lecithinase production, respectively, whereas 80.8% and 100% for L. innocua cultures were negative for the two test, respectively.

Keywords:Listeria monocytogenes; Hemolysin; Lecithinase; Congo red adsorption; Keratoconjunctivitis.

RESUMO

Com propósito de avaliar a produção de lecitinase e a capacidade de adsorção do corante vermelho Congo como marcadores de patogenicidade, foram estudadas 130 amostras de Listeria. Estas amostras foram identificadas segundo a produção de ácido a partir de açúcares aliada ao teste CAMP, correlacionando-se estes dados à capacidade de produção de ceratoconjuntivite em cobaio.

As culturas de L. monocytogenes apresentaram taxas de positividade para a adsorção do corante e produção de lecitinase de 51,8 e 88,8%, respectivamente, enquanto 80,8% e 100% das culturas de L. innocua foram negativas para os referidos testes.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

ACKNOWLEDGMENT

We thank Rosmery Ribeiro for the technical assistance.

Recebido para publicação em 08/10/1993

Aceito para publicação em 17/05/1994

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Publication Dates

  • Publication in this collection
    20 Sept 2006
  • Date of issue
    Aug 1994

History

  • Accepted
    17 May 1994
  • Received
    08 Oct 1993
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