Abstract
Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of cardiovascular diseases, especially in myocardial infarction and ischemia/reperfusion (I/R). However, the underlying molecular mechanism remains unclear. In this study, we determined the role and the possible underlying molecular mechanism of lncRNA-ROR in myocardial I/R injury. H9c2 cells and human cardiomyocytes (HCM) were subjected to either hypoxia/reoxygenation (H/R), I/R or normal conditions (normoxia). The expression levels of lncRNA-ROR were detected in serum of myocardial I/R injury patients, H9c2 cells, and HCM by qRT-PCR. Then, levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were measured by kits. Cell viability, apoptosis, apoptosis-associated factors, and p38/MAPK pathway were examined by MTT, flow cytometry, and western blot assays. Furthermore, reactive oxygen species (ROS) production was determined by H2DCF-DA and MitoSOX Red probes with flow cytometry. NADPH oxidase activity and NOX2 protein levels were measured by lucigenin chemiluminescence and western blot. Results showed that lncRNA-ROR expression was increased in I/R patients and in H/R treatment of H9c2 cells and HCM. Moreover, lncRNA-ROR significantly promoted H/R-induced myocardial injury via stimulating release of LDH, MDA, SOD, and GSH-PX. Furthermore, lncRNA-ROR decreased cell viability, increased apoptosis, and regulated expression of apoptosis-associated factors. Additionally, lncRNA-ROR increased phosphorylation of p38 and ERK1/2 expression and inhibition of p38/MAPK, and rescued lncRNA-ROR-induced cell injury in H9c2 cells and HCM. ROS production, NADPH oxidase activity, and NOX2 protein levels were promoted by lncRNA-ROR. These data suggested that lncRNA-ROR acted as a therapeutic agent for the treatment of myocardial I/R injury.
lncRNA; Ischemia/reperfusion (I/R); Hypoxia/reoxygenation (H/R); Cell viability; Apoptosis
Weiwei Zhang1, Ying Li2, Peng Wang1
1Department of Cardiology, Dezhou People’s Hospital, Dezhou, China
2Interventional Center, Dezhou People’s Hospital, Dezhou, China
Retraction for: Braz J Med Biol Res | doi: http://10.1590/1414-431X20186555 | PMID: 29694511 | PMCID: PMC5937723
The authors would like to retract the article “Long non-coding RNA-ROR aggravates myocardial ischemia/reperfusion injury” that was published in volume 51, no. 6 (2018) (Epub April 23, 2018) of the Brazilian Journal of Medical and Biological Research.
After the publication of this study, the corresponding author requested its retraction due to “the identification of data fabrication”. The Editors decided to retract this paper to avoid further damage to the scientific community.
The Brazilian Journal of Medical and Biological Research remains vigilant to prevent misconduct and reinforces the Journal’s commitment to good scientific practices. We regret the unprofessional behavior of the authors involved.
Correspondence: Weiwei Zhang: <zhangweiwei3832@126.com>
Publication Dates
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Publication in this collection
23 Apr 2018
History
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Received
15 Aug 2017 -
Accepted
25 Jan 2018
