Estudo da preservação do pulmão de cão mantido em autoperfusão

Fontes, Ronaldo D; Falzoni, Roberto; Magaldi, Ricardo; Kosai, Taro; Seguchi, Mário; Tarigoe, Marcelo; Stolf, Noedir A.G; Ramires, Antônio F; Jatene, Adib D

doi:10.1590/S0102-76381991000300009

Resumos

Foram estudados 14 blocos coração-pulmão (BCP), nos quais os pulmões foram mantidos em sistema de autoperfusão em período de duas até oito horas. Realizaram-se biópsias pulmonares a cada duas horas. Dez blocos mantiveram-se preservados até a quarta hora com pequena intensidade de alteração, demonstrada semiquantitativamente através de estudo estrutural. A partir desse tempo, edema intra-alveolar, hemorragia perivásculo-bronquial, hemorragia intra-alveolar, enfisema perivásculo-bronquia e rotura alveolar ocorreram com maior intensidade.

pulmão, preservação; sangue

Fourteen heart-lung blocks, kept in an autoperfusion system for a period of two to eight hours, were studied. Lung biopsies were performed each two hours. Ten lungs were preserved until four hours with slight degrees of alteration in the microscopic study. After the fourth hour, severe alterations developed and were represented by intra-alveolar edema, intra-alveolar haemorrhage, perivascular and peribronquial enfisema, and alveolar rupture.

lung preservation; blood

ARTIGOS ORIGINAIS

Estudo da preservação do pulmão de cão mantido em autoperfusão

Lung preservation in dogs kept under autoperfusion

Ronaldo D. Fontes; Roberto Falzoni; Ricardo Magaldi; Taro Kosai; Mário Seguchi; Marcelo Tarigoe; Noedir A.G. Stolf; Antônio F. Ramires; Adib D. Jatene

Do Instituto do Coração do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo

^{Endereço para separatas} Endereço para separatas: Ronaldo D. Fontes Divisão Cirúrgica Av. Dr. Eneas Carvalho de Aguiar, 44 05403, São Paulo, SP, Brasil

RESUMO

Foram estudados 14 blocos coração-pulmão (BCP), nos quais os pulmões foram mantidos em sistema de autoperfusão em período de duas até oito horas. Realizaram-se biópsias pulmonares a cada duas horas. Dez blocos mantiveram-se preservados até a quarta hora com pequena intensidade de alteração, demonstrada semiquantitativamente através de estudo estrutural. A partir desse tempo, edema intra-alveolar, hemorragia perivásculo-bronquial, hemorragia intra-alveolar, enfisema perivásculo-bronquia e rotura alveolar ocorreram com maior intensidade.

Descritores: pulmão, preservação; sangue, autotransfusão.

ABSTRACT

Fourteen heart-lung blocks, kept in an autoperfusion system for a period of two to eight hours, were studied. Lung biopsies were performed each two hours. Ten lungs were preserved until four hours with slight degrees of alteration in the microscopic study. After the fourth hour, severe alterations developed and were represented by intra-alveolar edema, intra-alveolar haemorrhage, perivascular and peribronquial enfisema, and alveolar rupture.

Descriptors: lung preservation; blood, autotransfusion.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Recebido para publicação em 13 de novembro de 1991.

Trabalho realizado no Instituto do Coração do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. São Paulo, SP, Brasil.

1 BLADES. B.; PIERPONT, A. C.; SANADI, A. - The effect of experimental lung ischemia on pulmonary function. Surg. Forum, 4:255-260, 1953.
2 BLUMENSTACIL, D.A.; HECHTMAN, H.B.; COLLINS, J.A. - Preservation of the canine lung. J. Thorac. Cardiovasc. Surg, 44:771-777, 1962.
3 BORRIE, J. & LICHTER, I. - Lung transplantation, technical problems. Torax, 19:383-385, 1965.
4 CONAUGHTON, P.L.; BANUTH, J.J.; LEWIS, F.J. - Lung, schemia up to six hours influence of local cooling in situ on subsequent pulmonary function. Dis. Chest, 41:400-404, 1962.
5 CRANE, R.; TORRES, M.; MAGSMON, J.W.C. - Twenty-four hour preservation and transplantation of the lung without functional impairment. Surg. Forum, 26:111-113, 1975.
6 FEELEY, T.W.; MIHN, F.G.; DOWNING, P.T.; SADEGHI, A.M.; BAUNGARTNER, W.A.; REITZ.; B.A.; SHUMWAY, N.E. Hypotermic preservation of the heart and lungs with Collins solution: effect on cardiorespiratory function in dogs. Ann. Thorac. Surg. 41: 301-307, 1986.
7 FONKALSRUD, E.N.; SANCHEZ, M.; LASSALETA, L. - Extendend preservation of the ichemic canine lung by ventilation with pump J. Surg. Res, 18:437-440, 1975.
8 GOLDSTEIN. S.; NOTILAGH, F.A.; TORRES, R.R. - Ventilatory and hemodynamic effects of long-term lung preservation and reimplantation. Surgery 19: 230-234, 1969.
9 HANKINSON, H.W. & EDWARDS, F.R. - The effect of pulmonary ischemic on lung function, Thorax, 14: 122-126, 1959.
10 HINO, K.; GROGAN, J.B.; HARDY, J.D. - Viability of stored lungs. Transplantation, 6: 25-28, 1968.
11 JOSEPH, W.L.; MORTHON, D.L.; ADKINS, P.C. - Influence of ischemia and hypotermia on the ability of the transplanted primate lung to provide immediate and total respiratory support. J. Thorac. Cardiovasc. Surg, 62: 752-756, 1971.
12 KONDO, Y.; TURNER, M.D.; COKRELL, J.V.; HARDY, J.D. - Ischemic tolerance of the canine allotransplanted lung. Surgery, 76: 147-149, 1974.
13 LARGIADER, F.; MANAY, W.G.; LYONS, G.W. - Exper. Homotransplant, 6: 25-29, 1968.
14 LARGIADER, F MANAY, W.G.; LYONS, G.W. - In vitro preservation of canine heart and lung. Arch. Surg, 91: 1-5, 1965.
15 LARGIADER, F.; MANAY, W.G.; LYONS, G.W. - Technical aspects of transplantation of preserved lungs. Chest, 49: 1-4, 1966.
16 LOWNSTEIN, E.; Preservation of excised canine lungs by ventilation with liquid fluorocarbon. Fed. Proc, 29: 1775-1779, 1970.
17 MODRY, D.L.; JIRSCH, D.W.; BOEHME, G. - Hypotermic perfusion of the isolated dog lung. Ann. Thorac. Surg, 16: 583-589, 1983.
18 OKANIWA, G.; NAKADA, T.; KAWAKANI, M. - Studies on preservation of canine lungs at subzero temperatures. J. Thorac. Cardiovasc. Surg, 65: 180-185, 1973.
19 STEVENS, G.H.; RANGEL, D.M.; YAKEISHI, Y. - The relationship of ventilation to preservation of the ischemic canine lung graft. Current Top. Surg. Res, 1: 51-57, 1969.
20 STEVENS, G.H.; SANCHES, M.N.; CHAPPEL, G.L. - Enhancement of lung preservation of lung collapse. J. Surg. Res, 14: 400-405, 1973.
21 TOLEDO-PEREYRA, L.H.; NORTHRUP, W.F.; HUMPHEREY, E.W. - Maintenance of lung viability for transplantation after long periods of hypotermic perfusion. J. Surg. Res, 18: 99-104, 1975.
22 VEITH, F.J.; DEYSINE, M.; NEHLSON, S.L. - Preservation of pulmonary function, hemodynamic and morphology in isolated perfused canine lungs. J. Thorac. Cardiovasc. Surg, 52: 37-41, 1966.
23 VEITH, F.J.; Do UGHERTY, J.C; ATTAI, L. - Lung, liver and Kidney preservation in transplantation. Transplant. Proc. 1: 808-812, 1969.
24 VEITH, F.J. & KOERNER, S.K. - The present status of lung transplantation. Arch. Surg, 109: 734-736, 1974.
25 VEITH, F.J.; KOERNER, S.K.; TORRES, M.; ATIAI, L.; GLIEDMAN, M.L. - Functional evaluation of lungs transplanted after varying ischemic intervals and after preservation by mechanical ventricular assistance. Ann. Thorac. Surg, 7: 446-449, 1969.
26 VEITH, F.J.; SIEGELMAN, S.; DOUGHERTY, J.C. - Long-term survival after lung autotransplantation and immediate contralateral pulmonary artery ligation. Surg. Gynecol. Obst, 133: 425-427, 1971.
27 VEITH, F.J.; SINHA, S.B.P.; GRAVES, J.S.; BOLEY, S.J.; DOUGUERTY, J.C. - Ischemic tolerance of the lung. J. Thorac. Cardiovasc. Surg, 61: 804-807, 1971.

Endereço para separatas:

Ronaldo D. Fontes

Divisão Cirúrgica

Av. Dr. Eneas Carvalho de Aguiar, 44

05403, São Paulo, SP, Brasil

Datas de Publicação

Publicação nesta coleção
17 Fev 2011
Data do Fascículo
Dez 1991

Histórico

Recebido
13 Nov 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1 BLADES. B.; PIERPONT, A. C.; SANADI, A. - The effect of experimental lung ischemia on pulmonary function. Surg. Forum, 4:255-260, 1953.

[2] 2 BLUMENSTACIL, D.A.; HECHTMAN, H.B.; COLLINS, J.A. - Preservation of the canine lung. J. Thorac. Cardiovasc. Surg, 44:771-777, 1962.

[3] 3 BORRIE, J. & LICHTER, I. - Lung transplantation, technical problems. Torax, 19:383-385, 1965.

[4] 4 CONAUGHTON, P.L.; BANUTH, J.J.; LEWIS, F.J. - Lung, schemia up to six hours influence of local cooling in situ on subsequent pulmonary function. Dis. Chest, 41:400-404, 1962.

[5] 5 CRANE, R.; TORRES, M.; MAGSMON, J.W.C. - Twenty-four hour preservation and transplantation of the lung without functional impairment. Surg. Forum, 26:111-113, 1975.

[6] 6 FEELEY, T.W.; MIHN, F.G.; DOWNING, P.T.; SADEGHI, A.M.; BAUNGARTNER, W.A.; REITZ.; B.A.; SHUMWAY, N.E. Hypotermic preservation of the heart and lungs with Collins solution: effect on cardiorespiratory function in dogs. Ann. Thorac. Surg. 41: 301-307, 1986.

[7] 7 FONKALSRUD, E.N.; SANCHEZ, M.; LASSALETA, L. - Extendend preservation of the ichemic canine lung by ventilation with pump J. Surg. Res, 18:437-440, 1975.

[8] 8 GOLDSTEIN. S.; NOTILAGH, F.A.; TORRES, R.R. - Ventilatory and hemodynamic effects of long-term lung preservation and reimplantation. Surgery 19: 230-234, 1969.

[9] 9 HANKINSON, H.W. & EDWARDS, F.R. - The effect of pulmonary ischemic on lung function, Thorax, 14: 122-126, 1959.

[10] 10 HINO, K.; GROGAN, J.B.; HARDY, J.D. - Viability of stored lungs. Transplantation, 6: 25-28, 1968.

[11] 11 JOSEPH, W.L.; MORTHON, D.L.; ADKINS, P.C. - Influence of ischemia and hypotermia on the ability of the transplanted primate lung to provide immediate and total respiratory support. J. Thorac. Cardiovasc. Surg, 62: 752-756, 1971.

[12] 12 KONDO, Y.; TURNER, M.D.; COKRELL, J.V.; HARDY, J.D. - Ischemic tolerance of the canine allotransplanted lung. Surgery, 76: 147-149, 1974.

[13] 13 LARGIADER, F.; MANAY, W.G.; LYONS, G.W. - Exper. Homotransplant, 6: 25-29, 1968.

[14] 14 LARGIADER, F MANAY, W.G.; LYONS, G.W. - In vitro preservation of canine heart and lung. Arch. Surg, 91: 1-5, 1965.

[15] 15 LARGIADER, F.; MANAY, W.G.; LYONS, G.W. - Technical aspects of transplantation of preserved lungs. Chest, 49: 1-4, 1966.

[16] 16 LOWNSTEIN, E.; Preservation of excised canine lungs by ventilation with liquid fluorocarbon. Fed. Proc, 29: 1775-1779, 1970.

[17] 17 MODRY, D.L.; JIRSCH, D.W.; BOEHME, G. - Hypotermic perfusion of the isolated dog lung. Ann. Thorac. Surg, 16: 583-589, 1983.

[18] 18 OKANIWA, G.; NAKADA, T.; KAWAKANI, M. - Studies on preservation of canine lungs at subzero temperatures. J. Thorac. Cardiovasc. Surg, 65: 180-185, 1973.

[19] 19 STEVENS, G.H.; RANGEL, D.M.; YAKEISHI, Y. - The relationship of ventilation to preservation of the ischemic canine lung graft. Current Top. Surg. Res, 1: 51-57, 1969.

[20] 20 STEVENS, G.H.; SANCHES, M.N.; CHAPPEL, G.L. - Enhancement of lung preservation of lung collapse. J. Surg. Res, 14: 400-405, 1973.

[21] 21 TOLEDO-PEREYRA, L.H.; NORTHRUP, W.F.; HUMPHEREY, E.W. - Maintenance of lung viability for transplantation after long periods of hypotermic perfusion. J. Surg. Res, 18: 99-104, 1975.

[22] 22 VEITH, F.J.; DEYSINE, M.; NEHLSON, S.L. - Preservation of pulmonary function, hemodynamic and morphology in isolated perfused canine lungs. J. Thorac. Cardiovasc. Surg, 52: 37-41, 1966.

[23] 23 VEITH, F.J.; Do UGHERTY, J.C; ATTAI, L. - Lung, liver and Kidney preservation in transplantation. Transplant. Proc. 1: 808-812, 1969.

[24] 24 VEITH, F.J. & KOERNER, S.K. - The present status of lung transplantation. Arch. Surg, 109: 734-736, 1974.

[25] 25 VEITH, F.J.; KOERNER, S.K.; TORRES, M.; ATIAI, L.; GLIEDMAN, M.L. - Functional evaluation of lungs transplanted after varying ischemic intervals and after preservation by mechanical ventricular assistance. Ann. Thorac. Surg, 7: 446-449, 1969.

[26] 26 VEITH, F.J.; SIEGELMAN, S.; DOUGHERTY, J.C. - Long-term survival after lung autotransplantation and immediate contralateral pulmonary artery ligation. Surg. Gynecol. Obst, 133: 425-427, 1971.

[27] 27 VEITH, F.J.; SINHA, S.B.P.; GRAVES, J.S.; BOLEY, S.J.; DOUGUERTY, J.C. - Ischemic tolerance of the lung. J. Thorac. Cardiovasc. Surg, 61: 804-807, 1971.