ABSTRACT:
Constant monitoring of milk quality by the processing industries demands the use of methodologies that add agility and low-cost analysis, such as the use of electronic equipment based on infrared or ultrasonic spectroscopy. In the present study, quality of raw milk received by dairy farms from western region of Santa Catarina state, Brazil, was evaluated throughout two methodologies of analysis of chemical composition and compared, being that both equipment used standard procedures for calibration, considering as reference data from the classic analytical methods recommended in Brazilian legislation. Milk samples from 45 producers were analyzed for SCC, TBC and physicochemical analysis, performed at dairy’s laboratory and at the official laboratory. Infrared and ultrasound methodologies for chemical composition of fat, protein and lactose were compared and correlated. The comparison of the methods showed that there was no significant difference for the mean values of fat (P=0.06); however, they presented significant difference for protein (P=0.001), higher for IR (3.23 vs. 3.33), and lactose (P<0.0001), higher for US (4.47 vs. 4.83). Correlation was positive and significant for fat (r=0.73, P<0.0001), protein (r=0.47, P=0.001) and lactose (r=0.51, P=0.0003). Considering the minimal composition required, TBC and SCC levels, 95.5%, 84.1% and 64.4% of the milk producers met the current Brazilian legislation, respectively. It was concluded that the methodologies have affected results, which may have been influenced by the correction factor used for equipment calibration, specifically for analyzed samples during the study period.
Key words:
composition; fast methods; fat; milk quality; protein
RESUMO:
O monitoramento constante da qualidade do leite por parte das indústrias beneficiadoras demanda o uso de metodologias que agregam agilidade e baixo custo por análise, como a utilização de equipamentos eletrônicos que podem ter como princípio a espectroscopia por infravermelho ou por ultrassom. No presente estudo, avaliou-se a qualidade do leite recebido por um laticínio da região extremo oeste catarinense e comparou-se duas metodologias de análise de composição química desta matéria prima, sendo que ambos os equipamentos utilizaram procedimentos padrões para calibração, considerando como referência os dados oriundos dos métodos analíticos clássicos preconizados na legislação Brasileira. Foram comparadas amostras de leite de 45 produtores para análise físico-química, de CCS e CBT, realizadas no laboratório do laticínio e em laboratório oficial. As metodologias por infravermelho (IR) e ultrassom (US) foram comparadas e correlacionadas para composição química de gordura, proteína e lactose. Os resultados demonstraram que não houve diferença significativa para as médias de gordura (P=0,06), entretanto, apresentaram diferença para proteína (P=0,001), maior para IR (3,23 vs. 3,33), e lactose (P<0,0001), maior para US (4,47 vs. 4,83). A correlação foi positiva e significativa para gordura (r=0,73, P<0,0001), proteína (r=0,47, P=0,001) e lactose (r=0,51, P=0,0003). Os resultados das análises de qualidade, considerando composição, CBT e CCS, atenderam a legislação vigente em 95,5%, 84,1% e 64,4% dos produtores, respectivamente. Concluiu-se que as metodologias afetaram os resultados, e estes podem ter sido influenciados pelo fator de correção, utilizado para calibração, sendo específicos para as amostras analisadas durante o período do estudo.
Palavras-chave:
composição do leite; gordura; métodos rápidos; proteína; qualidade de leite
INTRODUCTION:
Use of electronic methodologies in milk quality monitoring has gained space in the dairy chain because it provides quick and efficient responses on milk composition (HOLROYD, 2013HOLROYD, S.E. The use of near infrared spectroscopy on milk and milk products. Journal of Near Infrared Spectroscopy. 21:311-322.2013. Available from: <Available from: http://journals.sagepub.com/doi/10.1255/jnirs.1055
>. Accessed: Mar. 24, 2018. doi: 10.1255/jnirs.1055.
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) either for the dairy producer (TSENKOVA et al., 2000TSENKOVA, R. et al. Near infrared spectroscopy for biomonitoring cow milk composition measurement in a spectral region from 1,100 to 2,400 nanometers. Journal of Animal Science, v.78, p.515-522.2000. Available from: <Available from: https://doi.org/10.2527/2000.783515x
>. Accessed: Mar. 26, 2018. doi: 10.2527/2000.783515x.
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), or for the industry, in the receiving platform, in order to reach the best destination of the stocked milk and also the adequate use of parameters for quality payment (PONSANO et al., 2007PONSANO, E.H.G. et al. Correlação entre métodos tradicionais e espectroscopia de ultrassom na determinação de características físico-químicas do leite. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.59, p.1052-1057. 2007. Available from: <Available from: http://dx.doi.org/10.1590/S0102-09352007000400035
>. Accessed: Mar. 25, 2018. doi: 10.1590/S0102-09352007000400035.
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).Besides that, they are non-destructive methods that allow a large amount of analysis in a short period of time (BUCKIN et al., 2003BUCKIN, V. et al. Ultrasonic spectroscopy for material analysis: recent advances. Spectroscopy Europe, 15:20-25. 2003., TSENKOVA et al., 2000TSENKOVA, R. et al. Near infrared spectroscopy for biomonitoring cow milk composition measurement in a spectral region from 1,100 to 2,400 nanometers. Journal of Animal Science, v.78, p.515-522.2000. Available from: <Available from: https://doi.org/10.2527/2000.783515x
>. Accessed: Mar. 26, 2018. doi: 10.2527/2000.783515x.
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). Rapid milk analysis equipment can be based on the methods of infrared and ultrasonic spectroscopy.
Ultrasonic spectroscopy has as its principle the application of high frequency sound waves that print intermolecular forces to the materials of the system. Compression and decompression oscillating in the ultrassonic wave cause oscillations in the molecular arrangement of the sample, which responds with attraction or repulsion of intermolecular forces (BUCKIN et al., 2003BUCKIN, V. et al. Ultrasonic spectroscopy for material analysis: recent advances. Spectroscopy Europe, 15:20-25. 2003.); therefore, analyzed by transmission or reflection of the signals generated (BHARDWAJ, 2002BHARDWAJ,M.C. Non contact ultrasound: the final frontier in non destructive analysis. Boalsburg: Second Wave Systems, 2002. 50p.). On the other hand, the infrared spectroscopy is based on the ability of the specific chemical groups of some milk components to absorb infrared radiation at different wavelengths, such as fat, protein and lactose (BIGGS et al., 1987BIGGS, D.A.et al.. Analysis of fat, protein, lactose and total solids by infra-red absorption. In: Monograph on rapid indirect methods for measurement of the major components of milk. Bulletin of International Dairy Federation, v.208, p.21-29.1987.). Infrared spectroscopy is widely used to determine milk composition (HOLROYD, 2013HOLROYD, S.E. The use of near infrared spectroscopy on milk and milk products. Journal of Near Infrared Spectroscopy. 21:311-322.2013. Available from: <Available from: http://journals.sagepub.com/doi/10.1255/jnirs.1055
>. Accessed: Mar. 24, 2018. doi: 10.1255/jnirs.1055.
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), and is the standard method adopted by the Brazilian laboratories government to analyzed milk quality, denominated “Rede Brasileira de Laboratórios de Controle de Qualidade de Leite” (RBQL), as well as the method being adopted for use in the identification of adulterated milks (POONIA et al., 2017POONIA, A. et al. Detection of adulteration in milk: A review. International Journal of Dairy Technology, v.70, p.23-42. 2017. Available from: <Available from: https://doi.org/10.1111/1471-0307.12274
>. Accessed: Mar. 25, 2018. doi: 10.1111/1471-0307.12274.
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).
With the challenges currently found in the milk chain, these and other analyzes are essential for determining the quality and safety of the end products. In addition, they should be able to identify fraud or milk quality problems. The objective of this study was to evaluate the quality of the milk received by a dairy, compare and correlate two methodologies for analyzing the chemical composition of this raw material. Secondly, verify if the milk samples collected complies the actual legislation of Brazil.
MATERIALS AND METHODS:
Samples were collected between September 11 to 14, 2016, from five municipalities of the extreme west of Santa Catarina state, Brazil, as a probabilistic sampling carried out in 45 dairy producers, which delivery milk to the same dairy company. Samples were collected by trained dairy company staff, directly from the milk cooling tank of each dairy producer, where the following aliquots were collected: a sample with 300 mL of milk for the composition analyzes in the dairy company laboratory; a sample with 40 mL of milk in a sterile vial containing Bronopol (2-bromo-2-nitropropane-1,3-diol concentration between 0.017% to 0.023% m/v, and, natamycin between 0.00060% to 0.00093% m/v) for analysis of somatic cell count (SCC), fat, protein and lactose; and a sample with 40 mL in a sterile vial containing Azidiol (chloramphenicol concentration between 0.00045% to 0.00051% m/v, and, sodium azide between 0.011% to 0.012% m/v)for analysis of total bacterial count (TBC).Both of 40 mL vials were sent for analysis at the Dairy Herd Analysis Service (SARLE), University of Passo Fundo, to a laboratory accredited by the Ministry of Agriculture, Livestock and Supply, integrant of the Brazilian Network of Laboratories for Quality Control of Milk (RBQL). The vials containing the conservants (Azidiol and Bronopol)were homogenized by inversion until complete dissolution. All samples were remained under refrigeration (1-10 °C) until analysis.
Additionally, in the samples sent to the SARLE, analyzes of composition (fat, protein, lactose and total dry matter) were carried out using the mid-infrared radiation method (IR) (INTERNATIONAL..., 2013INTERNATIONAL DAIRY FEDERATION. Milk and liquid milk products - Guidelines for the application of mid-infrared spectrometry. IDF Standard 141 Second Edition. Brussels: International Dairy Federation .2013.) and somatic cell count by flow cytometry (CF) (INTERNATIONAL..., 2006INTERNATIONAL DAIRY FEDERATION. Milk: enumeration of somatic cell. IDF Standard 148-2 Second Edition. Brussels: International Dairy Federation . 2006.), by means of the electronic equipment Bentley Combi System 2300 ® , consisting of Bentley 2000 (BENTLEY..., 1998BENTLEY INSTRUMENTS INC.Bentley 2000 operator’s manual. Chaska, MN, EUA: Bentley InstrumentsInc., 1998. 79p.) and SomaCount 300 (BENTLEY..., 1997BENTLEY INSTRUMENTS INC.Somacount 300 operator’s manual. Chaska MN, EUA: BentleyInstruments Inc., 1997. 116p.), respectively. The analysis of TBC was performed by the same laboratory, using the technique of flow cytometry (CF) (INTERNATIONAL..., 2004INTERNATIONAL DAIRY FEDERATION. Milk - Quantitative determination of bacteriological quality - Guidance for establishing and verifying a conversion relationship between routine method results and anchor method results. IDF Standard 196 First Edition. Brussels: International Dairy Federation.2004.), by means of the electronic equipment IBC BactoCount 150 (BENTLEY..., 2007BENTLEY INSTRUMENTS INC. Bentley Bactocount IBC - User manual. Chaska MN, EUA: Bentley Instruments Inc., 2007. 142p.).
In parallel, the replicate of each sample was used for determination of fat, protein, lactose and density by the ultrasound method (US) using the portable ultrasonic milk analyzer (Ekomilk Total ® ) at dairy company laboratory. In addition to these, titratable acidity tests (BRASIL, 2006BRASIL. Instrução Normativa no. 68, de 12 de dezembro de 2006. Oficializar os Métodos Analíticos Oficiais Físico-Químicos, para Controle de Leite e Produtos Lácteos, em conformidade com o anexo desta Instrução Normativa, determinando que sejam utilizados nos Laboratórios Nacionais Agropecuários. DOU, 13/12/2006, Seção 1, p.8.2006.) and pH (with bench potentiometer, AKSO ®) were performed.
The instruments calibration and operation for raw milk analisys were performed according to ABNT NBR 17025 (2005)ASSOCIAÇÃO BRASILEIRA DE NORMAS TÉCNICAS. NBR ISO/IEC 17025:2005. Requisitos gerais para competência de laboratórios de ensaio e calibração. Rio de Janeiro, 2005. standards and BENTLEY (1997BENTLEY INSTRUMENTS INC.Somacount 300 operator’s manual. Chaska MN, EUA: BentleyInstruments Inc., 1997. 116p., 1998BENTLEY INSTRUMENTS INC.Bentley 2000 operator’s manual. Chaska, MN, EUA: Bentley InstrumentsInc., 1998. 79p.) manufacturer’s instructions. The official laboratory calibrates its equipment twice a month, while the company laboratory calibrates its equipment weekly. Both laboratories calibrate the equipment more often when necessary. The lab has a testing routine for sample quantity obtained from the supplier, comparing to the data bank values, to determine the calibration coefficient according to the Ministry of Agriculture, Normative Instruction No. 68 (Brazil, 2006BRASIL. Instrução Normativa no. 68, de 12 de dezembro de 2006. Oficializar os Métodos Analíticos Oficiais Físico-Químicos, para Controle de Leite e Produtos Lácteos, em conformidade com o anexo desta Instrução Normativa, determinando que sejam utilizados nos Laboratórios Nacionais Agropecuários. DOU, 13/12/2006, Seção 1, p.8.2006.).
Data on milk quality were presented in a descriptive way. To perform the mean tests, Student’s T or WillCockson tests were used, according to the normality and homoscedasticity of the variances analyzed by the Shapiro-Wilk and Bartlett tests, respectively. When possible, data transformations were performed using quadratic or logarithmic transformations. Pearson correlation was performed between the fat, protein and lactose variables, considering the infrared and ultrasound methods of analysis.
RESULTS AND DISCUSSION:
Considering the milk physicochemical composition, the comparison between ultrasonic (US) and infrared (IR) analysis did not show significant difference for milk fat (P>0.05). However, there was a significant difference for protein and lactose (Table 1). The results showed positive and significant correlations for fat (r=0.73, P<0.0001), protein (r=0.47, P=0.001) and lactose (r= 0.51, P=0.0003).In the US method, the parameters used in the analysis of the variation of the components are the attenuation and the velocity of the emitted sound (DUKHIN et al., 2003DUKHIN, A. S. et al. Ultrasound for characterizing liquid based food products. 1 - acoustic spectroscopy. Mount Kisco: Dispersion Technology. 2003. Available from: <Available from: http://www.dispersion.com/ultrasound-for-characterizing-liquid-based-food-products
>. Accessed: Mar. 12, 2018.
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). The concentration of fat is directly related to attenuation property, which is determined by the dispersion of ultrasonic waves in non-homogeneous samples, such as emulsions and suspensions (BUCKIN et al., 2003BUCKIN, V. et al. Ultrasonic spectroscopy for material analysis: recent advances. Spectroscopy Europe, 15:20-25. 2003.). Besides the lipid concentration, the degree of homogenization also influences the attenuation of the ultrasound, due to thermal losses resulting from the transfer of heat from the aqueous medium to the fat globules and contrariwise (MILES et al., 1990MILES, C.A. et al. Attenuation of ultrasound in milks and creams. Ultrasonics, v.28, p.394-400. 1990. Available from: <Available from: https://doi.org/10.1016/0041-624X(90)90063-T
>. Accessed: Mar., 26, 2018. doi: 10.1016/0041-624X(90)90063-T.
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). On the other hand, by the IR method the amount of radiation absorbed in the transmittance at specific wavelengths is recorded in the mid-infrared region (INTERNATIONAL..., 2013INTERNATIONAL DAIRY FEDERATION. Milk and liquid milk products - Guidelines for the application of mid-infrared spectrometry. IDF Standard 141 Second Edition. Brussels: International Dairy Federation .2013.), in which the carbonyl groups (C=O) of the triglyceride ester bonds absorb radiation at the wavelength of 5.723 μm and the carbon-hydrogen groups (C-H) of 3.48 μm (BARBANO & CLARK, 1989BARBANO, D.M. & CLARK, J.L. Infrared milk analysis - Challenges for the future. Journal of Dairy Science, v.72, p.1627-1636, 1989. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(89)79275-4
>. Accessed: Mar. 18, 2018. doi: 10.3168/jds.S0022-0302(89)79275-4.
https://doi.org/10.3168/jds.S0022-0302(8...
; LYNCH et al. 2006LYNCH,J.M.;et al. Precalibration evaluation procedures for mid-infrared milk analyzers. Journal of Dairy Science, v.89, p.2761-2774. 2006. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(06)72353-0
>. Accessed: Mar., 18, 2018. doi: 10.3168/jds.S0022-0302(06)72353-0.
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). Despite the possible influence of the homogenization of the sample on the results, there was no significant difference (P>0.05) between the methods for the means of fat (P=0.06), indicating absence of the influence of this factor in the present research. The results of the comparison of the evaluated methods for fat disagree with the results obtained by PINTO et al. (2008PINTO, A.T. et al. Correlação entre os métodos infravermelho e ultrassom na determinação da composição química do leite das vacas do concurso leiteiro da Expointer 2007. Acta Scientiae Veterinariae, v.36, p.273-276. 2008. Available from: <Available from: http://hdl.handle.net/10183/20719
>. Accessed: Mar. 25, 2018.
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), who found a significant difference in the comparison of the same methods (P=0.0005). On the other hand, its correlation was strong and positive (r=0.98) for this component, which agrees with the similar positive and significant correlation obtained in this study (r=0.73, P<0.0001).Similarly, in another study by MELO et al. (2018MELO, W. O. et al. Ultrasound spectroscopy as an alternative method to measure the physical-chemical constituents of buffalo milk. Ciência Rural, v.48, n.2, p.1678-1684. 2018. Available from: <Available from: http://www.scielo.br/scielo.php?pid=S010384782018000200753&script=sci_abstract&tlng=en
>. Accessed: Nov. 01, 2018. doi: 10.1590/0103-8478cr20170447.
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), using buffalo milk, it was possible to identify that there was also a significant difference in the comparison between the infrared and ultrasound in fat analysis, however, the correlation was also high and positive (r=0.84108, P<0.0001), which determines that ultrasound spectroscopy, can also be used in the evaluation of this component.
For protein, in the ultrasound spectroscopy the concentration of colloids is determined from the evaluation of the mechanical disturbances caused by the sound waves when passing through the sample (MILES et al., 1990MILES, C.A. et al. Attenuation of ultrasound in milks and creams. Ultrasonics, v.28, p.394-400. 1990. Available from: <Available from: https://doi.org/10.1016/0041-624X(90)90063-T
>. Accessed: Mar., 26, 2018. doi: 10.1016/0041-624X(90)90063-T.
https://doi.org/10.1016/0041-624X(90)900...
; BUCKIN et al., 2003BUCKIN, V. et al. Ultrasonic spectroscopy for material analysis: recent advances. Spectroscopy Europe, 15:20-25. 2003.; PONSANO et al., 2007PONSANO, E.H.G. et al. Correlação entre métodos tradicionais e espectroscopia de ultrassom na determinação de características físico-químicas do leite. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.59, p.1052-1057. 2007. Available from: <Available from: http://dx.doi.org/10.1590/S0102-09352007000400035
>. Accessed: Mar. 25, 2018. doi: 10.1590/S0102-09352007000400035.
http://dx.doi.org/10.1590/S0102-09352007...
). Somehow, it can be affirmed that the ultrasound is scattered and absorbed while propagates through the heterogeneous system (DUKHIN et al., 2003DUKHIN, A. S. et al. Ultrasound for characterizing liquid based food products. 1 - acoustic spectroscopy. Mount Kisco: Dispersion Technology. 2003. Available from: <Available from: http://www.dispersion.com/ultrasound-for-characterizing-liquid-based-food-products
>. Accessed: Mar. 12, 2018.
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), since milk is characterized as an emulsion of fat globules and a suspension of casein micelles in an aqueous phase, along with lactose, serum proteins and solubilized minerals (GONZÁLEZ, 2001GONZÁLEZ,F.H.D.. Composição bioquímica do leite e hormônios da lactação. In: GONZÁLEZ, F.H.D.; DÜRR, J.W.; FONTANELI, R.S.(Eds.) Uso do leite para monitorar a nutrição e o metabolismo de vacas leiteiras. Gráfica da Universidade Federal do Rio Grande do Sul, Porto Alegre. p.5-22.2001.). In the IR method, the amide groups (CONH) of the peptide bonds of the proteins absorb radiation at the wavelength at 6.465 μm (BARBANO & CLARK, 1989BARBANO, D.M. & CLARK, J.L. Infrared milk analysis - Challenges for the future. Journal of Dairy Science, v.72, p.1627-1636, 1989. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(89)79275-4
>. Accessed: Mar. 18, 2018. doi: 10.3168/jds.S0022-0302(89)79275-4.
https://doi.org/10.3168/jds.S0022-0302(8...
; LYNCH et al., 2006LYNCH,J.M.;et al. Precalibration evaluation procedures for mid-infrared milk analyzers. Journal of Dairy Science, v.89, p.2761-2774. 2006. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(06)72353-0
>. Accessed: Mar., 18, 2018. doi: 10.3168/jds.S0022-0302(06)72353-0.
https://doi.org/10.3168/jds.S0022-0302(0...
). The mean protein levels differed (P=0.001) between the two methods, but the correlation was positive and significant (r=0.47, P=0.001. As in fat, the mean protein content was higher in IR method compared to the US method. According to DÜRR et al. (2001DÜRR, J.W.et al. Determinação laboratorial dos componentes do leite. In: GONZÁLEZ, F.H.D.; DÜRR, J.W.; FONTANELI, R.S.(Eds.) Uso do leite para monitorar a nutrição e o metabolismo de vacas leiteiras. Gráfica da Universidade Federal do Rio Grande do Sul, Porto Alegre. p.23-29.2001.), in the determination of protein by the IR method, the lactic acid present in the sample is interpreted as protein, which may cause an overestimation of this total component, and therefore explain the significant difference found in the present research.
As well as the protein, the lactose is the dispersed phase in aqueous medium, being evaluated in the same way in the US method. But by the IR method, the hydroxyl groups (OH) of lactose absorb radiation at wavelengths of 9.610 μm (BARBANO & CLARK, 1989BARBANO, D.M. & CLARK, J.L. Infrared milk analysis - Challenges for the future. Journal of Dairy Science, v.72, p.1627-1636, 1989. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(89)79275-4
>. Accessed: Mar. 18, 2018. doi: 10.3168/jds.S0022-0302(89)79275-4.
https://doi.org/10.3168/jds.S0022-0302(8...
; LYNCH et al., 2006LYNCH,J.M.;et al. Precalibration evaluation procedures for mid-infrared milk analyzers. Journal of Dairy Science, v.89, p.2761-2774. 2006. Available from: <Available from: https://doi.org/10.3168/jds.S0022-0302(06)72353-0
>. Accessed: Mar., 18, 2018. doi: 10.3168/jds.S0022-0302(06)72353-0.
https://doi.org/10.3168/jds.S0022-0302(0...
). There was also a significant difference (P<0.0001) between the methodologies evaluated for this component, being the parameter that had the largest difference between the means and the only one that there was a decrease in the percentage in the IR in relation to the US. In fact, lactose is the component that is most altered with storage time due to its depletion in lactic acid, because of the action of microorganisms (DÜRR et al., 2001DÜRR, J.W.et al. Determinação laboratorial dos componentes do leite. In: GONZÁLEZ, F.H.D.; DÜRR, J.W.; FONTANELI, R.S.(Eds.) Uso do leite para monitorar a nutrição e o metabolismo de vacas leiteiras. Gráfica da Universidade Federal do Rio Grande do Sul, Porto Alegre. p.23-29.2001.). However, in this study the correlation between the methods was moderate (r=0.51, P=0.0003).
According to MARTINS et al. (2009MARTINS, M.E.P. et al. Conservantes bronopol e azidiol: influência do binômio tempo/temperatura na contagem bacteriana total do leite cru. Ciência Animal Brasileira, v.10, p.627-633.2009. Available from: <Available from: http://repositorio.bc.ufg.br/handle/ri/84
>. Accessed: Mar., 14, 2018.
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), the effect of the Bronopol can be influenced by the level of contamination of the sample, because according to its study, the conserve has as much bacteriostatic effect in samples of milk with TBC below 105 UFC/mL and bactericide in samples with TBC higher than 106 UFC/mL. Physiologically, the concentration of lactose is constant because it acts in the control of water inflow, due to its role in the maintenance of osmotic balance between milk and mammary gland (GONZÁLEZ, 2001GONZÁLEZ,F.H.D.. Composição bioquímica do leite e hormônios da lactação. In: GONZÁLEZ, F.H.D.; DÜRR, J.W.; FONTANELI, R.S.(Eds.) Uso do leite para monitorar a nutrição e o metabolismo de vacas leiteiras. Gráfica da Universidade Federal do Rio Grande do Sul, Porto Alegre. p.5-22.2001.), due to this fact, a low variation around the mean of the analyzes already corresponds to the statistical difference.
It is important to note that in ultrasonic spectroscopy it is possible to verify that the measurement of the transmitted energy is not affected by the multiple scattering, which occurs in infrared spectroscopy, for example. The forward ultrasonic propagation pulse precedes multiple scattering, however, it only decays (attenuation) due to single scattering, completely eliminating the multiple scattering problem, being one of the major advantages of ultrasound based techniques in relation to light, such as the infrared (DUKHIN et al., 2003DUKHIN, A. S. et al. Ultrasound for characterizing liquid based food products. 1 - acoustic spectroscopy. Mount Kisco: Dispersion Technology. 2003. Available from: <Available from: http://www.dispersion.com/ultrasound-for-characterizing-liquid-based-food-products
>. Accessed: Mar. 12, 2018.
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).
The chemical composition analyzes performed in the study (Table 2) showed that the results met the minimum parameters required by current legislation (BRASIL, 2011BRASIL. Instrução Normativa no. 62, de 29 de dezembro de 2011. Aprova o Regulamento Técnico de Produção, Identidade e Qualidade do Leite tipo A, o Regulamento Técnico de Identidade e Qualidade de Leite Cru Refrigerado, o Regulamento Técnico de Identidade e Qualidade de Leite Pasteurizado e o Regulamento Técnico da Coleta de Leite Cru Refrigerado e seu Transporte a Granel, em conformidade com os Anexos desta Instrução Normativa. DOU, 30/12/2011, Seção 1, p.6.2011.) in 95.5% (43) of the producers. In TBC, it was observed that the median was below the mean, which justifies the high standard deviation. However, the results were satisfactory, since 84.1% (37) of the samples were within the parameters required by the legislation (<300000 UFC/mL) and only 15.9% (7) of the samples were above the permitted level (>300000 UFC/mL) (BRASIL, 2016BRASIL. Instrução Normativa no. 7, de 03 de maio de 2016. Altera o Regulamento Técnico de Produção, Identidade e Qualidade do Leite tipo A, o Regulamento Técnico de Identidade e Qualidade de Leite Cru Refrigerado, o Regulamento Técnico da Coleta de Leite Cru Refrigerado e seu Transporte a Granel. DOU, 04/05/2016, Seção 1, p.11.2016.).
Physical-chemical and microbiological quality of milk, according to analyzes performed in an official laboratory of the Brazilian Network of Laboratories for Quality Control of Milk (RBQL, by the mid-infrared radiation method) and in a laboratory of a commercial dairy (by ultrasound method).
According to TRONCO (2010TRONCO, V.M. Manual de Inspeção da Qualidade do Leite. 4ª ed. Santa Maria: Editora UFSM. 2010.), for a normal udder state, the ideal is less than 250000 SC/mL. Only 15.6% of the samples could be considered inside the ideal state standards for the mammary gland. When the individual values are compared with current legislation, 64.4% (29) samples were inside the allowable parameters (<500000 SC/mL) (BRASIL, 2016), 26.7% (12) between 500000 and 750000 SC/mL, and only 8.9% (4) were above 750000 SC/mL. According to VOGES et al. (2015VOGES, J.G. et al. Qualidade do leite e a sua relação com o sistema de produção e a estrutura para ordenha. Revista Brasileira de Ciência Veterinária, v.22, p.171-175.2015. Available from: <Available from: https://doi.org/10.22409/rbcv.v22i3-4.728
>. Accessed: Mar. 25, 2018. doi: 10.22409/rbcv.v22i3-4.728.
https://doi.org/10.22409/rbcv.v22i3-4.72...
), there is a positive relationship between TBC and SCC, which determines that the same factors that affect the microbiological quality of milk affect the health of the mammary gland, justifying that properties with hygiene problems are very likely to have problems also with subclinical mastitis. It can be confirmed by the CHRAMOSTOVÁ et al (2016CHRAMOSTOVÁ, J. et al. Proteolysis in raw milk in relation to microbiological indicators. Czech Journal of Food Science, v.34, p.306-312. 2016. Available from: <Available from: https://www.agriculturejournals.cz/publicFiles/64_2016-CJFS.pdf
>. Accessed: Nov. 10, 2018. doi: 10.17221/64/2016-CJFS.
https://www.agriculturejournals.cz/publi...
) study, that found a positive and significant correlation (P<0.05) between the total mesophilic bacteria count and somatic cell count (SCC) (r=0.3126).
In this sense, of the 7 producers with high TBC, all of them also presented SCC above adequate levels for a healthy mammary gland, which according to TRONCO (2010TRONCO, V.M. Manual de Inspeção da Qualidade do Leite. 4ª ed. Santa Maria: Editora UFSM. 2010.), 250000 SC/mL. In addition to the sanitary parameters involved in high SCC, it also influences the accuracy of determination by infrared spectroscopy, where studies by TSENKOVA et al. (2001TSENKOVA R. et al. Near-infrared spectroscopy for biomonitoring - influence of somatic cell count on cow’s milk composition analysis. International Dairy Journal, v.11, p.779-783.2001.Available from: <Available from: https://doi.org/10.1016/S0958-6946(01)00110-8
>. Accessed: Mar. 25, 2018. doi: 10.1016/S0958-6946(01)00110-8.
https://doi.org/10.1016/S0958-6946(01)00...
) concluded that the use of high SCC milk samples in a dataset for calibration or prediction strongly influenced the accuracy of fat, protein and lactose determination, increasing the standard error of prediction and significantly reducing the correlation coefficient. Therefore, it is necessary to use milk with low SCC for calibration of equipment based in IR method, situation which suggests research on the influence of SCC on the analysis also by US.
MELO et al., (2018MELO, W. O. et al. Ultrasound spectroscopy as an alternative method to measure the physical-chemical constituents of buffalo milk. Ciência Rural, v.48, n.2, p.1678-1684. 2018. Available from: <Available from: http://www.scielo.br/scielo.php?pid=S010384782018000200753&script=sci_abstract&tlng=en
>. Accessed: Nov. 01, 2018. doi: 10.1590/0103-8478cr20170447.
http://www.scielo.br/scielo.php?pid=S010...
) indicate that the differences between results of the determination of protein and lactose of buffalo milk for the infrared method and ultrasonic analyser is possibly due to calibration, use of preservatives and time between the collection and samples analysis. In this way the observed difference between the results obtained by the infrared ultrasonic methodology can be related to the calibration correction factor, used for each method, considering each analytical procedure, as well as, the samples used to compose this factor, which are not necessarily from the same group
CONCLUSION:
The comparison of the ultrasonic and infrared methodologies in the milk analysis showed differences in the results, and the positive correlation between the methodologies is regular. Considering that two out of three evaluated parameters differed (protein and lactose).
The ultrasonic and infrared methodologies comparison in the milk analysis showed differences in the results, and the positive correlation between the methodologies is regular. Considering that two out of three evaluated parameters differed (protein and lactose), it is suggested that the processing industries use only one of the methodologies for decision-making process. Yet more research about the influence of composition, time of the year and frequency of collection for analysis on both methods, is required.
ACKNOWLEDGEMENTS
We thank the “Programa de Bolsas Universitárias de Santa Catarina UNIEDU/Pós Graduação” and the “Programa de Bolsas de Monitoria de Pós-Graduação - PROMOP” for supporting master’s degree of the first author of this paper through the Scholarship Program.
This project was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brasil - Finance code 001.
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CR-2018-0510.R1
Publication Dates
-
Publication in this collection
04 July 2019 -
Date of issue
2019
History
-
Received
25 June 2018 -
Accepted
04 Apr 2019 -
Reviewed
17 June 2019