Métodos de cultura de algas do plâncton marinho: estudos realizados nas regiões de Cananéia e de Ubatuba, SP

Vieira, Armando A. H.

doi:10.1590/S0373-55241977000200008

Resumo

The study of algae culture collected in Brazilian waters was proposed due to the lack of literature on the subject: an attempt was made to develop a methodology to obtain algae culture and its purification. Several species were isolated from samples collected a Ubatuba and Cananéia (São Paulo) (Lat. 23º30'S - Long. 45º06'W and Lat. 25º01'S - Long. 47º54'W) by means of phytoplankton net and Van Dorn bottle. The following isolation techniques were employed after washing and concentration: glass capillary, inoculation of mixed cultures on Petri dishes with solid medium and positive phototropism. The isolated algae were kept in five different mediuns. Washing techniques were tested (centrifugation, "filter tubes" and re-isolation) , antibiotics and ultra violet radiation to obtain unialgal bacteria-free cultures were employed to obtain pure cultures. Five différents antibiotics, mixed in différents concentrations, were employed during several periods of expositions. The re-isolation proved to be the best method to obtain the purification of the cultures.

Métodos de cultura de algas do plâncton marinho: estudos realizados nas regiões de Cananéia e de Ubatuba, SP

Culture methods of planktonic marine algae: studies in Cananéia and Ubatuba regions

Armando A. H. Vieira^* * Contratado pelo FINEP para o "Projeto Integrado para Uso e Exploração Racional do Ambiente Marinho".

Instituto Oceanográfico da Universidade de São Paulo

SYNOPSIS

The study of algae culture collected in Brazilian waters was proposed due to the lack of literature on the subject: an attempt was made to develop a methodology to obtain algae culture and its purification.

Several species were isolated from samples collected a Ubatuba and Cananéia (São Paulo) (Lat. 23º30'S - Long. 45º06'W and Lat. 25º01'S - Long. 47º54'W) by means of phytoplankton net and Van Dorn bottle.

The following isolation techniques were employed after washing and concentration: glass capillary, inoculation of mixed cultures on Petri dishes with solid medium and positive phototropism.

The isolated algae were kept in five different mediuns. Washing techniques were tested (centrifugation, "filter tubes" and re-isolation) , antibiotics and ultra violet radiation to obtain unialgal bacteria-free cultures were employed to obtain pure cultures. Five différents antibiotics, mixed in différents concentrations, were employed during several periods of expositions. The re-isolation proved to be the best method to obtain the purification of the cultures.

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AGRADECIMENTO

Agradeço à Direção do Instituto Oceanográfico da Universidade de São Paulo, pelo apoio e pelas facilidades dispensadas na utilização de suas instalações, e à FAPESP, pelo apoio financeiro de parte deste trabalho.

BIBLIOGRAFIA

BALANTINE, D. 1953. Comparison of the different methods of estimating nannoplankton J. mar. biol. Ass. U.K., 32(1):129-142.

CASTELLVI, J. 1971. Contribución a la biologia de S. costatum (Grev.) Cleve. Investigación pesq., 35(2):365-520 .

CHU, S. P. 1947 . Note on the technique of making bacteria free cultures of marine diatoms. J. mar. biol. Ass. U.K., 26:296-302.

DROOP, M. R. 1967. A procedure for routine purification of algal cultures with antibiotics. Br. phycol. Bull., 3(2):295-297.

DROOP, M. R. 1969. Algae. In: Norris, T. R. & Ribbons, D. W., ed.- Methods in microbiology. London, Academic Press, p.270-308.

FISH, G. R. 1950. A method of obtaining bacteria-free cultures of a marine flagellate and Enteromorpha intestinalis using penicillin. Acta Horti gothoburg, 18:82-89.

GERLOFF, G. C; FITZGERALD, P. & SKOOG, F. 1950. The isolation, purification, and culture of blue-green algae. Am. J. Bot., 37:216-218.

GREEN, B. R. 1974. Nucleic acids and their metabolism. In: Stewart, W.D.P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.281-313.

GROSS, F. 1937. Notes on the culture of some marine organisms. J. mar. biol. Ass. U.K., 21(2):753-768.

GUILLARD, R. R. L. 1973. Methods for microflagellates and nannoplankton. In: Stein, J. R., ed.- Handbook of phycological methods. Culture Methods and Growth Measurements. Cambridge, University Press, 69-85.

______ & RYTHER, J. H. 1962. Studies of marine plankton diatoms. I. Cyclotella hustedt and Detonula oonfervacea (Cleve) Gran. Can. J. Microbiol., 8:229-239.

HARVEY, H. W. 1955. The chemistry and fertility of sea waters . London, Cambridge University Press, viii + 224p.

KAIN, J. M. & FOGG, G. E. 1960. Studies on the growth of marine phy top lank ton. III. Prorocentrun micans Ehrengerg. J. mar. biol. Ass. U.K., 39:33-50.

KUMAR, H. D. 1964. Streptomycin and penicillin induced inhibition of growth and pigment production in blue-green algae and production of strains of Anacystis nidulans resistant to these antibiotics. J. exp. Bot., 15:232-250.

KUTNER, M. B. B. 1972. Variação estacional e distribuição do fitoplâncton na região de Cananéia. Tese de doutoramento. São Paulo, Instituto de Biociencias da Universidade de São Paulo, 104p.

LEWIN, R. A., ed. 1962. Physiology and biochemistry of algae. New York, Academic Press, 929p.

LLOYD, D. 1974. Dark respiration. In: Stewart, W.D.P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.505-529.

LORENZEN, H. & HESSE, M. 1974. Synchronous cultures. In: Stewart, W. D. P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.894-908.

MINGOIA, Q. 1967. Química farmacêutica. Sao Paulo, Melhoramentos/ EDUSP, 787p.

PAREDES, J. F. 1967-1968. Some methods used to obtain bacteria-free cultures of marine phytoplankton. Mems Inst. Invest, cient. Mogamb., 9, (A), (Ciências Biológicas), p.127-141.

PRAKASH, A.; RASHID, M.A.; JENSEN, A. & SUBRA RAO, D.V. 1973. Influence of humic substances on the growth of marine phytoplankton: diatoms. Limnol. Oceanogr., 18(4).516-524.

PRINGSHEIM, E. G. 1946. Pure cultures of algae. Cambridge, Univ. Press, London, 119p.

PROVASOLI, L.; McLAUGHLIN, J. J. A. & DROOP, M. R. 1957. The development of artificial media for marine algae. Arch. Mikrobiol., 25:392-425.

QURAISHI, F. O. 1968. The reactions of phytoplankton to different illumination regimes. Ph. D. thesis. Univ. Wales (Bangor).

SAGER, R. 1974. Nuclear and cytoplasmatic inheritance in green algae. In: Stewart, W. D. P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p. 314-345.

SPENCER, C. P. 1952. On the use of antibiotic for isolating bacteriafree cultures of marine phytoplankton organisms. J. mar. biol. Ass. U.K., 21:87-106.

STEEMANN-NIELSEN, E. 1955. The production of antibiotics by plankton algae and its effects upon bacterial activities in the sea. Pap. in Mar. Biol, and Oceanogr. Deep Sea Res., 3Suppl.,281p.

______ 1964. On a complication in marine productivity work due to the influence of ultraviolet light. J. Cons. perm. int. Explor. Mer, 29(2):130-135.

UMEBÁYASHI, O. 1972. Preservation of some culture diatoms. Bull. Tokai reg. Fish Res. Lab., (69):55-62.

ZEHNDER, A. & HUGHES, E. O. 1958. The antialgal activity of actidione. Can. J. Microbiol., 4:399-408.

ZOBELL, C. E. & LONG, J. H. 1938. Studies on the isolation of bacteria-free culture marine phytoplankton. J. mar. Res., 1:328-333.

(Received July 21/1976)

BALANTINE, D. 1953. Comparison of the different methods of estimating nannoplankton J. mar. biol. Ass. U.K., 32(1):129-142.
CASTELLVI, J. 1971. Contribución a la biologia de S. costatum (Grev.) Cleve. Investigación pesq., 35(2):365-520 .
CHU, S. P. 1947 . Note on the technique of making bacteria free cultures of marine diatoms. J. mar. biol. Ass. U.K., 26:296-302.
DROOP, M. R. 1967. A procedure for routine purification of algal cultures with antibiotics. Br. phycol. Bull., 3(2):295-297.
DROOP, M. R. 1969. Algae. In: Norris, T. R. & Ribbons, D. W., ed.- Methods in microbiology. London, Academic Press, p.270-308.
FISH, G. R. 1950. A method of obtaining bacteria-free cultures of a marine flagellate and Enteromorpha intestinalis using penicillin. Acta Horti gothoburg, 18:82-89.
GERLOFF, G. C; FITZGERALD, P. & SKOOG, F. 1950. The isolation, purification, and culture of blue-green algae. Am. J. Bot., 37:216-218.
GREEN, B. R. 1974. Nucleic acids and their metabolism. In: Stewart, W.D.P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.281-313.
GROSS, F. 1937. Notes on the culture of some marine organisms. J. mar. biol. Ass. U.K., 21(2):753-768.
GUILLARD, R. R. L. 1973. Methods for microflagellates and nannoplankton. In: Stein, J. R., ed.- Handbook of phycological methods. Culture Methods and Growth Measurements. Cambridge, University Press, 69-85.
______ & RYTHER, J. H. 1962. Studies of marine plankton diatoms. I. Cyclotella hustedt and Detonula oonfervacea (Cleve) Gran. Can. J. Microbiol., 8:229-239.
HARVEY, H. W. 1955. The chemistry and fertility of sea waters . London, Cambridge University Press, viii + 224p.
KAIN, J. M. & FOGG, G. E. 1960. Studies on the growth of marine phy top lank ton. III. Prorocentrun micans Ehrengerg. J. mar. biol. Ass. U.K., 39:33-50.
KUMAR, H. D. 1964. Streptomycin and penicillin induced inhibition of growth and pigment production in blue-green algae and production of strains of Anacystis nidulans resistant to these antibiotics. J. exp. Bot., 15:232-250.
KUTNER, M. B. B. 1972. Variação estacional e distribuição do fitoplâncton na região de Cananéia. Tese de doutoramento. São Paulo, Instituto de Biociencias da Universidade de São Paulo, 104p.
LEWIN, R. A., ed. 1962. Physiology and biochemistry of algae. New York, Academic Press, 929p.
LLOYD, D. 1974. Dark respiration. In: Stewart, W.D.P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.505-529.
LORENZEN, H. & HESSE, M. 1974. Synchronous cultures. In: Stewart, W. D. P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.894-908.
PRAKASH, A.; RASHID, M.A.; JENSEN, A. & SUBRA RAO, D.V. 1973. Influence of humic substances on the growth of marine phytoplankton: diatoms. Limnol. Oceanogr., 18(4).516-524.
PRINGSHEIM, E. G. 1946. Pure cultures of algae. Cambridge, Univ. Press, London, 119p.
PROVASOLI, L.; McLAUGHLIN, J. J. A. & DROOP, M. R. 1957. The development of artificial media for marine algae. Arch. Mikrobiol., 25:392-425.
QURAISHI, F. O. 1968. The reactions of phytoplankton to different illumination regimes. Ph. D. thesis. Univ. Wales (Bangor).
SAGER, R. 1974. Nuclear and cytoplasmatic inheritance in green algae. In: Stewart, W. D. P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p. 314-345.
SPENCER, C. P. 1952. On the use of antibiotic for isolating bacteriafree cultures of marine phytoplankton organisms. J. mar. biol. Ass. U.K., 21:87-106.
STEEMANN-NIELSEN, E. 1955. The production of antibiotics by plankton algae and its effects upon bacterial activities in the sea. Pap. in Mar. Biol, and Oceanogr. Deep Sea Res., 3Suppl.,281p.
______ 1964. On a complication in marine productivity work due to the influence of ultraviolet light. J. Cons. perm. int. Explor. Mer, 29(2):130-135.
UMEBÁYASHI, O. 1972. Preservation of some culture diatoms. Bull. Tokai reg. Fish Res. Lab., (69):55-62.
ZEHNDER, A. & HUGHES, E. O. 1958. The antialgal activity of actidione. Can. J. Microbiol., 4:399-408.
ZOBELL, C. E. & LONG, J. H. 1938. Studies on the isolation of bacteria-free culture marine phytoplankton. J. mar. Res., 1:328-333.

*

Contratado pelo FINEP para o "Projeto Integrado para Uso e Exploração Racional do Ambiente Marinho".

Datas de Publicação

Publicação nesta coleção
06 Jun 2012
Data do Fascículo
1977

Histórico

Recebido
21 Jul 1976

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] BALANTINE, D. 1953. Comparison of the different methods of estimating nannoplankton J. mar. biol. Ass. U.K., 32(1):129-142.

[2] CASTELLVI, J. 1971. Contribución a la biologia de S. costatum (Grev.) Cleve. Investigación pesq., 35(2):365-520 .

[3] CHU, S. P. 1947 . Note on the technique of making bacteria free cultures of marine diatoms. J. mar. biol. Ass. U.K., 26:296-302.

[4] DROOP, M. R. 1967. A procedure for routine purification of algal cultures with antibiotics. Br. phycol. Bull., 3(2):295-297.

[5] DROOP, M. R. 1969. Algae. In: Norris, T. R. & Ribbons, D. W., ed.- Methods in microbiology. London, Academic Press, p.270-308.

[6] FISH, G. R. 1950. A method of obtaining bacteria-free cultures of a marine flagellate and Enteromorpha intestinalis using penicillin. Acta Horti gothoburg, 18:82-89.

[7] GERLOFF, G. C; FITZGERALD, P. & SKOOG, F. 1950. The isolation, purification, and culture of blue-green algae. Am. J. Bot., 37:216-218.

[8] GREEN, B. R. 1974. Nucleic acids and their metabolism. In: Stewart, W.D.P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.281-313.

[9] GROSS, F. 1937. Notes on the culture of some marine organisms. J. mar. biol. Ass. U.K., 21(2):753-768.

[10] GUILLARD, R. R. L. 1973. Methods for microflagellates and nannoplankton. In: Stein, J. R., ed.- Handbook of phycological methods. Culture Methods and Growth Measurements. Cambridge, University Press, 69-85.

[11] ______ & RYTHER, J. H. 1962. Studies of marine plankton diatoms. I. Cyclotella hustedt and Detonula oonfervacea (Cleve) Gran. Can. J. Microbiol., 8:229-239.

[12] HARVEY, H. W. 1955. The chemistry and fertility of sea waters . London, Cambridge University Press, viii + 224p.

[13] KAIN, J. M. & FOGG, G. E. 1960. Studies on the growth of marine phy top lank ton. III. Prorocentrun micans Ehrengerg. J. mar. biol. Ass. U.K., 39:33-50.

[14] KUMAR, H. D. 1964. Streptomycin and penicillin induced inhibition of growth and pigment production in blue-green algae and production of strains of Anacystis nidulans resistant to these antibiotics. J. exp. Bot., 15:232-250.

[15] KUTNER, M. B. B. 1972. Variação estacional e distribuição do fitoplâncton na região de Cananéia. Tese de doutoramento. São Paulo, Instituto de Biociencias da Universidade de São Paulo, 104p.

[16] LEWIN, R. A., ed. 1962. Physiology and biochemistry of algae. New York, Academic Press, 929p.

[17] LLOYD, D. 1974. Dark respiration. In: Stewart, W.D.P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.505-529.

[18] LORENZEN, H. & HESSE, M. 1974. Synchronous cultures. In: Stewart, W. D. P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p.894-908.

[21] PRAKASH, A.; RASHID, M.A.; JENSEN, A. & SUBRA RAO, D.V. 1973. Influence of humic substances on the growth of marine phytoplankton: diatoms. Limnol. Oceanogr., 18(4).516-524.

[22] PRINGSHEIM, E. G. 1946. Pure cultures of algae. Cambridge, Univ. Press, London, 119p.

[23] PROVASOLI, L.; McLAUGHLIN, J. J. A. & DROOP, M. R. 1957. The development of artificial media for marine algae. Arch. Mikrobiol., 25:392-425.

[24] QURAISHI, F. O. 1968. The reactions of phytoplankton to different illumination regimes. Ph. D. thesis. Univ. Wales (Bangor).

[25] SAGER, R. 1974. Nuclear and cytoplasmatic inheritance in green algae. In: Stewart, W. D. P., ed.- Algal physiology and biochemistry. Oxford, Blackwell, p. 314-345.

[26] SPENCER, C. P. 1952. On the use of antibiotic for isolating bacteriafree cultures of marine phytoplankton organisms. J. mar. biol. Ass. U.K., 21:87-106.

[27] STEEMANN-NIELSEN, E. 1955. The production of antibiotics by plankton algae and its effects upon bacterial activities in the sea. Pap. in Mar. Biol, and Oceanogr. Deep Sea Res., 3Suppl.,281p.

[28] ______ 1964. On a complication in marine productivity work due to the influence of ultraviolet light. J. Cons. perm. int. Explor. Mer, 29(2):130-135.

[29] UMEBÁYASHI, O. 1972. Preservation of some culture diatoms. Bull. Tokai reg. Fish Res. Lab., (69):55-62.

[30] ZEHNDER, A. & HUGHES, E. O. 1958. The antialgal activity of actidione. Can. J. Microbiol., 4:399-408.

[31] ZOBELL, C. E. & LONG, J. H. 1938. Studies on the isolation of bacteria-free culture marine phytoplankton. J. mar. Res., 1:328-333.

Brasil

Brasil

Métodos de cultura de algas do plâncton marinho: estudos realizados nas regiões de Cananéia e de Ubatuba, SP

Culture methods of planktonic marine algae: studies in Cananéia and Ubatuba regions

Resumo

Datas de Publicação

Histórico