Abstract
Calcium sulphoaluminate cement (CSAC) is an attractive candidate for biomedical applications due to its appropriate mechanical properties and high calcium content. In vitro bioactivity and hemocompatibility of calcium sulphoaluminate cement were assessed. The cement was prepared from a mixture of calcium sulphoaluminate (CSA) clinker, gypsum and water. Cement samples were immersed in a simulated body fluid (SBF) at 37 °C for different periods of time (7, 14 and 21 days). The analyses of these samples after their immersion in SBF revealed the formation of a bonelike apatite layer on their surface. The hemolytic activity was assessed by measuring hemoglobin released from erythrocytes when they were exposed to the cement. The results showed that the cement is hemocompatible.
bone cements; calcium sulphoaluminate; bioactive cements
1 Introduction
Typically, a bone cement is used to mechanically fix implants to bone. However, in order to have a cement able to bond chemically to bone it is necessary to develop a bioactive material that stimulate the regeneration of osseous tissue. The bond mechanism of implant-tissue is based on the formation of a bioactive calcium phosphate layer on the implant surface by reaction and ion exchange. The ability to form a bioactive layer at the interface between material and living tissue was observed in 1969 and this phenomenon was defined as bioactivity11 Coleman NJ, Nicholson JW and Awosanya K. A preliminary investigation of the vitro bioactivity of white Portland cement. Cement and Concrete Research. 2007; 37(11):1518-1523. http://dx.doi.org/10.1016/j.cemconres.2007.08.008.
http://dx.doi.org/10.1016/j.cemconres.20...
.
Since the discovery of Bioglass®, which promotes a rapid osseointegration22 Ohtsuki C, Kokubo T and Yakamuro T. Mechanism of apatite formation on CaO-SiO2-P2O glasses in a simulated body fluid. 5Journal of Non-Crystalline Solids. 1992; 143:84-92. http://dx.doi.org/10.1016/S0022-3093(05)80556-3.
http://dx.doi.org/10.1016/S0022-3093(05)...
,33 Kokubo T. Bioactive glass ceramics: properties and applications. Biomaterials. 1991; 12(2):155-163. http://dx.doi.org/10.1016/0142-9612(91)90194-F. PMid:1878450
http://dx.doi.org/10.1016/0142-9612(91)9...
, a great variety of bioactive materials for medical applications, such as glasses, glass-ceramics, ceramics and composites, have been developed44 Oyane A, Onuma K, Ito A, Kim H-M, Kokubo T and Nakamura T. Formation and growth of clusters in conventional and new kinds of simulated body fluids. Journal of Biomedical Materials Research. Part A. 2003; 64(2):339-348. http://dx.doi.org/10.1002/jbm.a.10426. PMid:12522821
http://dx.doi.org/10.1002/jbm.a.10426...
,55 Siriphannon P, Kameshima Y, Yasumori A, Okada K and Hayashi S. Formation of hydroxyapatite on CaSiO powders in simulated body fluid. 3Journal of the European Ceramic Society. 2002; 22(4):511-520. http://dx.doi.org/10.1016/S0955-2219(01)00301-6.
http://dx.doi.org/10.1016/S0955-2219(01)...
. The main requisites of a bioactive material are biocompatibility, appropriate mechanical properties and the ability to form an HA layer on its surface66 Sandra E. Rodil. Modificación superficial de biomateriales metálicos. Revista Latinoamericana de Metalurgia y Materiales. 2009; 29(2):67-83..
The application of biomaterials depends upon their interfacial properties and resultant interactions with cells and biological fluids in vivo77 Cai X, Yuan J, Chen S, Li P, Li L and Shen J. Hemocompatibility improvement of poly(ethylene terephthalate) via self-polymerization of dopamine and covalent graft of zwitterions. Materials Science and Engineering: C. 2014; 36:42-48. http://dx.doi.org/10.1016/j.msec.2013.11.038. PMid:24433885
http://dx.doi.org/10.1016/j.msec.2013.11...
. The interaction of red blood cells with biomaterials or their extracts may be useful to evaluate the hemolytic activity of materials. For decades, evidence of the in vitro hemolysis has been used to identify the biocompatibility properties of biomaterials88 Henkelman S, Rakhorst G, Blanton J and van Oeveren W. Standardization of incubation conditions for hemolysis testing of biomaterials. Materials Science and Engineering: C. 2009; 29(5):1650-1654. http://dx.doi.org/10.1016/j.msec.2009.01.002.
http://dx.doi.org/10.1016/j.msec.2009.01...
.
A great variety of bone cements have been developed and they are used for fixing, reparation or replacement of bone99 Santos LA, Carrodéguas RG, Rogero SO, Higa OZ, Boschi AO and Arruda ACF. α-tricalcium phosphate cement: “in vitro” cytotoxicity. Biomaterials. 2002; 23(9):2035-2042. http://dx.doi.org/10.1016/S0142-9612(01)00333-7. PMid:11996045
http://dx.doi.org/10.1016/S0142-9612(01)...
. In recent years, research has been focused on the development of calcium phosphate cements1010 Guo H, Wei J and Liu CS. Development of a degradable cement of calcium phosphate and calcium sulfate composite for bone reconstruction. Biomedical Materials (Bristol, England). 2006; 1(4):193-197. http://dx.doi.org/10.1088/1748-6041/1/4/003. PMid:18458405
http://dx.doi.org/10.1088/1748-6041/1/4/...
,1111 Hernández Q and Piña Barba C. Caracterización física y química de pastas de cementos óseos con ZrO2. Revista Mexicana de Física. 2003; 49:123-131., however the low mechanical strength of these materials limits their use to replace bone in high load bearing applications. Additionally, calcium aluminates cements have been used as filler materials in dental and orthopedic applications1212 Medri V, Mazzocchi M and Bellosi A. Doped calcium-aluminium-phosphate cements for biomedical applications. Journal of Materials Science. Materials in Medicine. 2011; 22(2):229-236. http://dx.doi.org/10.1007/s10856-010-4205-3. PMid:21165760
http://dx.doi.org/10.1007/s10856-010-420...
. These cements show long setting time (135 min) and low compressive strength (46.33 MPa for 24h), which limit their use in some medical applications1313 Oh SH, Choi S, Lee YK and Kim KN. Preparation of calcium aluminate cement for hard tissue repair: effects of lithium fluoride and maleic acid on setting behavior, compressive strength, and biocompatibility. Journal of Biomedical Materials Research. 2002; 62(4):593-599. http://dx.doi.org/10.1002/jbm.10347. PMid:12221708
http://dx.doi.org/10.1002/jbm.10347...
.
The calcium sulphoaluminate cement develops early high strengths1414 Liao Y, Wei X and Li G. Early hydration of calcium sulfoaluminate cement through electrical resistivity measurement and microstructure investigations. Construction & Building Materials. 2011; 25(4):1572-1579. http://dx.doi.org/10.1016/j.conbuildmat.2010.09.042.
http://dx.doi.org/10.1016/j.conbuildmat....
, higher than those reported for calcium aluminate and calcium phosphate cements. This high strength is due to the formation of ettringite (Ca6Al2(SO4)3(OH)12
.26H2O) during hydration of CSAC. In addition, these cements have a short setting time in the range of 45-60 min without any accelerating additive. Due to this and taking into account that no bioactive sulphoaluminate cements have been reported, in this work the in vitro bioactivity and hemocompatibility of a calcium sulphoaluminate cement were assessed.
2 Experimental
2.1 Cement preparation
The clinker of calcium sulphoaluminate (CSA) was obtained from a mixture of reagent grade chemicals (Sigma Aldrich) of calcium carbonate (CaCO3), alumina (Al2O3) and hemi-hydrated gypsum (CaSO4·1/2H2O). The mixture was homogenized in a plastic jar with alumina balls in acetone for 4 h using milling rods, then the mixture was dried at 80 °C, deagglomerated and heat treated at 1350 °C for 4 h. The obtained clinker was milled in a porcelain jar with alumina balls until reaching a surface area of 531 m2/kg with an average particle size of 12 μm. The obtained clinker was analyzed by X-ray diffraction (XRD; Philips, Xpert). The cement (CSAC) was prepared by mixing the clinker (80 wt-%) with CaSO4·1/2H2O (20 wt-%), then water was added using a water/cement ratio of 0.5 and casted into nylamid molds of 1 cm in diameter and 0.5 cm in height. Samples were set for 1 h at room temperature and assessed for in vitro bioactivity. After hydration the samples were immersed in ethanol for 1 h and dried for 24 h at room temperature and then were analyzed by XRD.
2.2 In vitro bioactivity assessment
The SBF was prepared following the procedure described by Kokubo & Takadama1515 Kokubo T and Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials. 2006; 27(15):2907-2915. http://dx.doi.org/10.1016/j.biomaterials.2006.01.017. PMid:16448693
http://dx.doi.org/10.1016/j.biomaterials...
. Appropriate amounts of the following reagent grade chemicals were dissolved into deionized water: NaCl, NaHCO3, KCl, K2HPO4·3H2O, MgCl2·6H2O, HCl, CaCl2 and Na2SO4; tris(hidroximetil)-aminometane was used to adjust pH to 7.4 at 37 °C.
After setting, samples were immersed in 200 ml of SBF in a polyethylene flask and placed into an incubator at 37 °C for 7, 14 and 21 days under sessile conditions. The area to volume ratio was 1.57 mm2/ml. After immersion, samples were soaked in ethanol for 1 h to stop any other reaction and then dried for 24 h and stored in a desiccator. Cement samples were analyzed before and after immersion in SBF by scanning electron microscopy (SEM; Philips, XL30 ESEM), energy dispersive spectroscopy (EDS; EDAX, Pegasus), Fourier transformed-infrared spectroscopy (FT-IR; Nicolet, Avatar 360) and XRD. For comparison purposes, a CSAC sample was hydrated in water for 21 days and characterized by FT-IR.
The calcium, phosphorus, sulphur and aluminum concentrations of the remaining SBF’s were evaluated after 1, 7, 14 and 21 days of immersion of CSAC samples by inductively-coupled plasma atomic emission spectrometry (ICP-AES; Thermo Elemental, IRIS Intrepid II XSP). The pH values of the remaining SBF’s were also monitored at different periods of time (Thermo Orion 420 pH meter).
2.3 Hemolysis tests
Hemolysis is defined as the release of hemoglobin due to the damage to the erythrocytes membrane. This type of testing allows the in vitro evaluation of the hemolytic activity of biomaterials or their extracts. In the hemolysis tests both extract and cement were assessed. The aim of hemolysis testing of the extract was to determine if the cement leachate products were hemolytic, while the objective of the hemolysis testing of cement was to determine if the direct contact between the cement surface and red blood cells may cause hemolysis.
Fresh human blood from a volunteer donor was obtained. The blood was collected in tubes with anticoagulant (EDTA) and centrifuged at 3200 rpm for 4 min. The obtained pellets were washed three times with phosphate-buffered saline (PBS). The supernatant was then removed and 1 ml of the purified erythrocytes was diluted with PBS (1:9). The cement specimens were prepared by mixing clinker (80 wt-%) with CaSO4·1/2H2O (20 wt-%), then water was added using a water/cement ratio of 0.5 and the paste was casted into nylamid molds of 0.6 cm in diameter and 1.4 cm in height. Specimens were hydrated in SBF for 24 h at 37 °C before testing. The hemolysis tests were performed placing the specimens into tubes with a volume of 1800 µl of PBS for 30 min at 37 °C in a water bath. In the case of the cement extract tests, the specimens were taken out of the tubes. Then, 200 µl of the blood solution were added to the tubes containing the extract or the specimens and were incubated for 1 h at 37 °C in a water bath. A positive and a negative controls are required in this test. The negative control (0% hemolysis) was prepared by adding an isotonic solution; in this case PBS was used. The positive control (100% hemolysis) was prepared by adding deionized water. According to the ASTM F756 standard, PBS is used as a blank in the hemolysis testing due to the fact that this solution keeps pH and the anticoagulant properties of the chelating agents used in collecting the blood. PBS is not hemolytic. Blood cells require an isotonic medium; it means a solution with the same amount of solute than cells. PBS is a common solution used to preserve cells. However, when cells are exposed to a hypotonic medium, a lower solute concentration outside the cells is present. This fact leads to a water absorption by cells in order to compensate the solute imbalance, leading to the expansion and bursting of cells, releasing hemoglobin. Deionized water is a hypotonic medium due to its very low amount of solutes.
Six experimental replicates were used for each group. After incubation, samples were centrifuged at 3200 rpm for 4 min at room temperature to collect the supernatant. The absorbance (A) value of the hemoglobin released from the erythrocyte cells was measured spectrophotometrically at 545 nm. Hemolysis percentage was calculated as follows:
3 Results and Discussion
Figure 1 shows the XRD patterns corresponding to the clinker of CSA (1a) and to the cement after hydration (1b). The only detected phase in the clinker was calcium sulphoaluminate (4CaO·3Al2O3·SO3) (Figure 1a) and the main hydration product corresponded to ettringite (Ca6Al2(SO4)3(OH)12 .26H2O) (Figure 1b). There was an indication that the hydration was not completed since some CSA remained without reaction.
Figure 2 shows the calcium, phosphorus and sulphur concentrations (Figure 2a) and the pH behavior (Figure 2b) of the remaining SBF’s as a function of immersion time. Initially, the calcium and sulphur concentrations increase in the SBF and then keep almost constant up to 21 days of immersion. This increase is due to the partial dissolution of ettringite in SBF. In contrast, the concentration of phosphorus decreases. The depletion of phosphorus in SBF may be due to the formation of a Ca, P-rich compound on the CSAC. On the other hand, aluminum in the remaining SBF’s was detected after only 21 days of immersion showing a concentration of 0.015 ppm. Reported values of Al in human plasma of healthy subjects indicated that the content of this element varied from 0.003 to 0.039 ppm1616 Wawschinek O, Petek W, Lang J, Pogglitsch H and Holzer H. The determination of aluminium in human plasma. Mikrochimica Acta. 1982; 77(5-6):335-339. http://dx.doi.org/10.1007/BF01197113.
http://dx.doi.org/10.1007/BF01197113...
. The Al concentration in the remaining SBF of this work, 0.015 ppm, is within this range. The ettringite dissolution leads to the formation of Al(OH)3 (solid)1717 Kirsimäe K, Kaasik A, Vohla C, Mõtlep R and Mander U. Hydrated calcareous oil-shale ash as potential filter media for phosphorus removal in constructed wetlands. Water Research. 2008; 42(4-5):1315-1323. http://dx.doi.org/10.1016/j.watres.2007.10.002. PMid:17959214
http://dx.doi.org/10.1016/j.watres.2007....
and Ca2+ and SO4
2-. The pH values of SBF increased from 7.4 (before immersion) to 7.6 after 21 days of immersion. This slight change in pH indicates that an ionic exchange has occurred.
(a) Ca, P and S concentration and (b) pH values of the remaining SBF after 1, 7, 14 and 21 days of contact with CSAC.
SEM images and corresponding EDS spectra of the cement surface before and after immersion in SBF (for 7, 14, and 21 days) are shown in Figure 3. Figure 3a1 shows the CSAC surface before immersion in SBF. A compact and rough morphology is observed. The corresponding EDS spectrum (Figure 3a2) shows the main elements of this kind of cement, Ca, S, Al and O. Figures 3b1, 3c1 and 3d1 show that a Ca, P-rich layer has been formed on the cement surface after 7, 14 and 21 days of immersion in SBF. In all the cases, the morphology of this compound consists of spherical agglomerates similar to those formed on the existing bioactive systems. As the immersion time increases, the spherical agglomerates increase in size. The EDS spectrum of each sample (Figures 3b2, 3c2 and 3d2) showed that the peak corresponding to Al decreases as the immersion time is increased and, at the same time, that of P increases. The materials that are able to form a Ca, P-rich compound while in contact with SBF are considered as bioactive.
SEM and EDS analysis of the cement samples (a) before immersion and after immersion in SBF for (b) 7, (c) 14 and (d) 21 days.
Figure 4 shows the FT-IR spectra of the CSAC hydrated in water for 21 days (Figure 4a), the stoichiometric hydroxyapatite (Figure 4b) and the CSAC after immersion in SBF for 21 days (Figure 4c). In Figure 4a the bands within the ranges of 1100-1200 cm–1 and 600-700 cm–1 correspond to S-O and Al-O bonds, respectively. These bonds are present from octahedrally co-ordinated AlO6 units in ettringite and to the CSAC1818 Gastaldi D, Canonico F and Boccaleri E. Ettringite and calcium sulfoaluminate cement: investigation of water content by near-infrared spectroscopy. Journal of Materials Science. 2009; 44(21):5788-5794. http://dx.doi.org/10.1007/s10853-009-3812-1.
http://dx.doi.org/10.1007/s10853-009-381...
,1919 Fernández-Carrasco L, Torrens-Martín D, Morales LM and Martínez-Ramírez S. Infrared spectroscopy in the analysis of building and construction materials. In: Theophile T, editor. Infrared spectroscopy: materials science, engineering and technology. In Tech; 2012. p. 369-382. Chapter 19. Available from: <http://www.intechopen.com/books/infrared-spectroscopy-materials-science-engineering-and-technology/infrared-spectroscopy-of-cementitious-materials>.. In this spectrum (Figure 4a), the bands observed in the ranges of 1600-1700 cm–1 and 3400-3600 cm–1, corresponding to O-H vibrations and bound water, are attributed to ettringite. In Figure 4b (HA spectrum) the presence of wavebands in the range of 1000-1100 cm–1 correspond to the P-O stretching vibrations2020 Fathi MH, Hanifi A and Mortazavi V. Preparation and bioactivity evaluation of bone-like hydroxyapatite nanopowder. Journal of Materials Processing Technology. 2008; 202(1-3):536-542. http://dx.doi.org/10.1016/j.jmatprotec.2007.10.004.
http://dx.doi.org/10.1016/j.jmatprotec.2...
, as well as the bands located within 565-601 cm–1 (P-O bending modes). In this spectrum, the bands corresponding to the O-H vibrations are found in the range of 3400-3600 cm–1. In Figure 4c (spectrum of the cement after immersion in SBF) the wavebands in the range of 1025-1040 cm–1 and within 565 and 601 cm–1 correspond to the P-O bending modes. Bands corresponding to the O-H vibrations in the range of 3400-3600 cm–1 were also observed. According to these two spectra (4b, c), it is evident that the formation of a bonelike apatite on the CSAC after immersion in SBF has been occurred.
FT-IR spectra of (a) CSAC hydrated in water for 21 days, (b) stoichiometric hydroxyapatite and (c) CSAC after immersion in SBF for 21 days.
Figure 5 shows the XRD patterns corresponding to the CSAC after 14 and 21 days of immersion in SBF (Figures 5a, b, respectively). After 14 and 21 days of immersion in SBF, the phase corresponding to hydroxyapatite [024-0033; Ca5(PO4)3(OH)] was identified, Figures 5a, b. The ettringite phase was not detected after these immersion time periods. A further research is needed to completely understand the mechanism of apatite formation on this calcium sulphoaluminate cement. However, the release of Ca ions from the cement to the SBF due to the partial dissolution of ettringite increases pH leading to the nucleation of apatite on the cement. Kirsimäe et al.1717 Kirsimäe K, Kaasik A, Vohla C, Mõtlep R and Mander U. Hydrated calcareous oil-shale ash as potential filter media for phosphorus removal in constructed wetlands. Water Research. 2008; 42(4-5):1315-1323. http://dx.doi.org/10.1016/j.watres.2007.10.002. PMid:17959214
http://dx.doi.org/10.1016/j.watres.2007....
studied the phosphorous elimination from solutions using hydrated calcareous ash sediments that contains ettringite. They stated that agglomerates of calcium phosphate may be formed on the ettringite crystals. Also, they suggested that, as the ettringite structure consists of columns and channels, there are suitable spaces for a number of ions and ion complexes (PO4
–3, AsO4
–3, etc.) to either interact with the ettringite surface or to be ionically exchanged for sulphate ions into the structural tunnels.
Figure 6 shows the results of hemolysis percentage for the extract and the specimens of the calcium sulphoaluminate cement. It is observed that there is no hemolytic activity for the CSAC extract (0.6% of hemolysis) and only a slight hemolytic activity for the specimens (4.4% of hemolysis). These results demonstrated that the CSAC is hemocompatible (less than 5% hemolysis) according to the ASTM F756 standard2121 American Society for Testing and Materials - ASTM. ASTM F-756: standard practice for assessment of hemolytic properties of materials. West Conshohocken; 2000..
Hemolysis percentages of the extract and the specimen of the calcium sulphoaluminate cement.
4 Conclusions
A Ca, P-rich compound was formed on the cement as early as 7 days of immersion in SBF. The morphology of this compound is similar to that formed on the existing bioactive systems. Furthermore, the Ca, P-rich layer was identified as apatite. The bioactivity of this cementive system makes this material a potential novel bone cement. The Ca ions release from the cement to the SBF due to the partial dissolution of ettringite and the increase in pH enhances the bonelike apatite formation. Ettringite crystals may be acting as preferred sites for apatite nucleation. Calcium sulphoaluminate cement demonstrated to be hemocompatible (less than 5% hemolysis2121 American Society for Testing and Materials - ASTM. ASTM F-756: standard practice for assessment of hemolytic properties of materials. West Conshohocken; 2000.) after the in vitro hemolysis testing.
Acknowledgements
The authors gratefully acknowledge CONACYT, México for the provision of the scholarship for I.O. Acuña Gutiérrez.
References
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1Coleman NJ, Nicholson JW and Awosanya K. A preliminary investigation of the vitro bioactivity of white Portland cement. Cement and Concrete Research. 2007; 37(11):1518-1523. http://dx.doi.org/10.1016/j.cemconres.2007.08.008
» http://dx.doi.org/10.1016/j.cemconres.2007.08.008 -
2Ohtsuki C, Kokubo T and Yakamuro T. Mechanism of apatite formation on CaO-SiO2-P2O glasses in a simulated body fluid. 5Journal of Non-Crystalline Solids. 1992; 143:84-92. http://dx.doi.org/10.1016/S0022-3093(05)80556-3
» http://dx.doi.org/10.1016/S0022-3093(05)80556-3 -
3Kokubo T. Bioactive glass ceramics: properties and applications. Biomaterials. 1991; 12(2):155-163. http://dx.doi.org/10.1016/0142-9612(91)90194-F PMid:1878450
» http://dx.doi.org/10.1016/0142-9612(91)90194-F -
4Oyane A, Onuma K, Ito A, Kim H-M, Kokubo T and Nakamura T. Formation and growth of clusters in conventional and new kinds of simulated body fluids. Journal of Biomedical Materials Research. Part A. 2003; 64(2):339-348. http://dx.doi.org/10.1002/jbm.a.10426 PMid:12522821
» http://dx.doi.org/10.1002/jbm.a.10426 -
5Siriphannon P, Kameshima Y, Yasumori A, Okada K and Hayashi S. Formation of hydroxyapatite on CaSiO powders in simulated body fluid. 3Journal of the European Ceramic Society. 2002; 22(4):511-520. http://dx.doi.org/10.1016/S0955-2219(01)00301-6
» http://dx.doi.org/10.1016/S0955-2219(01)00301-6 -
6Sandra E. Rodil. Modificación superficial de biomateriales metálicos. Revista Latinoamericana de Metalurgia y Materiales. 2009; 29(2):67-83.
-
7Cai X, Yuan J, Chen S, Li P, Li L and Shen J. Hemocompatibility improvement of poly(ethylene terephthalate) via self-polymerization of dopamine and covalent graft of zwitterions. Materials Science and Engineering: C. 2014; 36:42-48. http://dx.doi.org/10.1016/j.msec.2013.11.038 PMid:24433885
» http://dx.doi.org/10.1016/j.msec.2013.11.038 -
8Henkelman S, Rakhorst G, Blanton J and van Oeveren W. Standardization of incubation conditions for hemolysis testing of biomaterials. Materials Science and Engineering: C. 2009; 29(5):1650-1654. http://dx.doi.org/10.1016/j.msec.2009.01.002
» http://dx.doi.org/10.1016/j.msec.2009.01.002 -
9Santos LA, Carrodéguas RG, Rogero SO, Higa OZ, Boschi AO and Arruda ACF. α-tricalcium phosphate cement: “in vitro” cytotoxicity. Biomaterials. 2002; 23(9):2035-2042. http://dx.doi.org/10.1016/S0142-9612(01)00333-7 PMid:11996045
» http://dx.doi.org/10.1016/S0142-9612(01)00333-7 -
10Guo H, Wei J and Liu CS. Development of a degradable cement of calcium phosphate and calcium sulfate composite for bone reconstruction. Biomedical Materials (Bristol, England). 2006; 1(4):193-197. http://dx.doi.org/10.1088/1748-6041/1/4/003 PMid:18458405
» http://dx.doi.org/10.1088/1748-6041/1/4/003 -
11Hernández Q and Piña Barba C. Caracterización física y química de pastas de cementos óseos con ZrO2. Revista Mexicana de Física. 2003; 49:123-131.
-
12Medri V, Mazzocchi M and Bellosi A. Doped calcium-aluminium-phosphate cements for biomedical applications. Journal of Materials Science. Materials in Medicine. 2011; 22(2):229-236. http://dx.doi.org/10.1007/s10856-010-4205-3 PMid:21165760
» http://dx.doi.org/10.1007/s10856-010-4205-3 -
13Oh SH, Choi S, Lee YK and Kim KN. Preparation of calcium aluminate cement for hard tissue repair: effects of lithium fluoride and maleic acid on setting behavior, compressive strength, and biocompatibility. Journal of Biomedical Materials Research. 2002; 62(4):593-599. http://dx.doi.org/10.1002/jbm.10347 PMid:12221708
» http://dx.doi.org/10.1002/jbm.10347 -
14Liao Y, Wei X and Li G. Early hydration of calcium sulfoaluminate cement through electrical resistivity measurement and microstructure investigations. Construction & Building Materials. 2011; 25(4):1572-1579. http://dx.doi.org/10.1016/j.conbuildmat.2010.09.042
» http://dx.doi.org/10.1016/j.conbuildmat.2010.09.042 -
15Kokubo T and Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials. 2006; 27(15):2907-2915. http://dx.doi.org/10.1016/j.biomaterials.2006.01.017 PMid:16448693
» http://dx.doi.org/10.1016/j.biomaterials.2006.01.017 -
16Wawschinek O, Petek W, Lang J, Pogglitsch H and Holzer H. The determination of aluminium in human plasma. Mikrochimica Acta. 1982; 77(5-6):335-339. http://dx.doi.org/10.1007/BF01197113
» http://dx.doi.org/10.1007/BF01197113 -
17Kirsimäe K, Kaasik A, Vohla C, Mõtlep R and Mander U. Hydrated calcareous oil-shale ash as potential filter media for phosphorus removal in constructed wetlands. Water Research. 2008; 42(4-5):1315-1323. http://dx.doi.org/10.1016/j.watres.2007.10.002 PMid:17959214
» http://dx.doi.org/10.1016/j.watres.2007.10.002 -
18Gastaldi D, Canonico F and Boccaleri E. Ettringite and calcium sulfoaluminate cement: investigation of water content by near-infrared spectroscopy. Journal of Materials Science. 2009; 44(21):5788-5794. http://dx.doi.org/10.1007/s10853-009-3812-1
» http://dx.doi.org/10.1007/s10853-009-3812-1 -
19Fernández-Carrasco L, Torrens-Martín D, Morales LM and Martínez-Ramírez S. Infrared spectroscopy in the analysis of building and construction materials. In: Theophile T, editor. Infrared spectroscopy: materials science, engineering and technology. In Tech; 2012. p. 369-382. Chapter 19. Available from: <http://www.intechopen.com/books/infrared-spectroscopy-materials-science-engineering-and-technology/infrared-spectroscopy-of-cementitious-materials>.
-
20Fathi MH, Hanifi A and Mortazavi V. Preparation and bioactivity evaluation of bone-like hydroxyapatite nanopowder. Journal of Materials Processing Technology. 2008; 202(1-3):536-542. http://dx.doi.org/10.1016/j.jmatprotec.2007.10.004
» http://dx.doi.org/10.1016/j.jmatprotec.2007.10.004 -
21American Society for Testing and Materials - ASTM. ASTM F-756: standard practice for assessment of hemolytic properties of materials. West Conshohocken; 2000.
Publication Dates
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Publication in this collection
Mar-Apr 2015
History
-
Received
16 June 2014 -
Reviewed
11 Jan 2015