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Computer vision applied to food and agricultural products

Visão computacional aplicada a alimentos e produtos agrícolas

ABSTRACT

Computer vision (CV) has been applied for years to automate many human activities. It is one of the key technologies for the modernization of the agri-food industry towards the fourth industrial revolution (Industry 4.0). In the agricultural sector, CV systems are applied to automate or obtain information from many agricultural tasks such as planting, cultivation, farm management, disease control, weed control or robotic harvesting. It is also widely used in postharvest to automate and obtain objective information in processes such as quality control and evaluation, damage detection, classification of fruits or vegetables in commercial categories or composition analysis. One of the main advantages is the ability of this technology to obtain information in regions of the spectrum that are invisible to the human eye. An example is the case of hyperspectral imaging systems. These systems generate a large amount of data that needs to be processed efficiently, creating robust and repeatable statistical models that allow the technology to be implemented at an industrial level. To achieve this, it is necessary to couple CV systems with advanced artificial intelligence tools such as machine learning or deep learning. The objective of this work is to review the latest advances in CV systems applied to food and agricultural products and processes.

Key words:
Digital images; Machine vision; Agriculture 4.0; Machine learning; Artificial intelligence

RESUMO

A visão computacional (CV) tem sido aplicada há anos para automatizar muitas atividades humanas. É uma das tecnologias-chave para a modernização da indústria agroalimentar em direção à quarta revolução industrial (Indústria 4.0). No setor agrícola, sistemas CV são aplicados para automatizar ou obter informações de muitas tarefas agrícolas, como plantio, cultivo, gerenciamento de fazenda, controle de doenças, controle de ervas daninhas ou colheita robótica. Também é amplamente utilizado em pós-colheita para automatizar e obter informações objetivas em processos como controle de qualidade e avaliação , detecção de danos, classificação de frutas ou vegetais em categorias comerciais ou análise de composição. Uma das principais vantagens é a capacidade desta tecnologia de obter informações em regiões do espectro invisíveis ao olho humano. Um exemplo é o caso de sistemas de imagens hiperespectrais. Esses sistemas geram uma grande quantidade de dados que precisam ser processados de forma eficiente, criando modelos estatísticos robustos e repetíveis que permitam a tecnologia a ser implementada a nível industrial. Para isso, é necessário acoplar os sistemas de CV a ferramentas avançadas de inteligência artificial, como aprendizado de máquina ou aprendizado profundo. O objetivo deste trabalho é revisar os últimos avanços em sistemas de CV aplicados a alimentos e produtos e processos agrícolas.

Palavras-chave:
Imagens digitais; Visão de máquina; Agricultura 4.0; Aprendizado de máquina; Inteligência artificial

INTRODUCTION

The main objectives of future agriculture are to increase productivity and food quality, reduce operations costs, and optimize input use. Therefore, the development of computer vision and its application in the development of non-destructive methods, precision agriculture, etc., enables us to automate and accelerate field, harvest, and post-harvest operations, essentially creating a new branch of Industry 4.0 called Agriculture 4.0. This type of agriculture integrates data and information to monitor field activities by applying remote and proximal sensing (PALLOTTINO et al., 2019PALLOTTINO, F. et al. Optoelectronic proximal sensing vehicle-mounted technologies in precision agriculture: A review. Computers and Electronics in Agriculture, v. 162, p. 859-873, 2019.).

Computer vision allows several activities. Recently is being researched fruit count on orchards (although the occlusion), disease plant detection, and defects detection in fruits. CV also improves the robot’s capacity to determine the fruit harvest point, and nowadays, CV is further used to identify and estimate fruit weight supermarkets.

In addition, consumer interest in food quality and safety is increasing, primarily owing to international food trade, which requires rapid and non-destructive inspection methods (OK et al., 2019OK, G. et al. Large-scan-area sub-terahertz imaging system for non-destructive food quality inspection. Food Control, v. 96, p. 383-389, 2019.). Similarly, the prediction of quality parameters, identification of adulteration and variety, discrimination of origin, etc., are activities of interest in the evaluation of agri-food products but are currently based on offline and destructive techniques (WANG; SUN; PU, 2017WANG, K.; SUN, D. W.; PU, H. Emerging non-destructive terahertz spectroscopic imaging technique: Principle and applications in the agri-food industry. Trends in Food Science & Technology, v. 67, p. 93–105, 2017.).

The development of sensors has enabled us to obtain big data in a non-destructive manner, reducing analysis costs and time. Several sensors that detect and monitor electromagnetic waves combined with new techniques of image processing, machine vision, and computer science are used to build smart systems for Agriculture 4.0.

Based on this, this review presents state-of-the-art computer vision systems for proximal sensing (food and agricultural products close or in contact with sensors), including the actual type of systems, processing, and applications.

BASIS OF COMPUTER VISION

Computer vision systems, coupled with artificial intelligence (AI), have become more critical to the use of the Internet of Things and its recent applications in the agriculture and food industry. These systems enable machines to distinguish and understand the actual world; AI is a tool that enables machines to perform tasks that humans can do, combining observing, interpreting, elucidating, and problem-solving simultaneously as the machine interacts with the environment. This means that machines can receive external inputs and adapt their activities appropriately, becoming what are known as smart machines (GOLLAPUDI, 2019GOLLAPUDI, S. Learn Computer Vision Using OpenCV: With Deep Learning CNNs and RNNs. Berkeley, CA: Apress, 2019.).

Smart machines in agriculture reduce the farmer’s costs because robots can operate without a break and perform tasks such as plowing, sowing, fruit harvesting, and pesticide spraying. Machines and equipment perform operations using digital visual data such as images from commercial cameras, graphical data, video, and heat intensity maps; these constitute the computer vision’s core input. The machines include smartphones, drones, closed-circuit televisions, magnetic resonance imaging (MRI) scanners, different sensors, and visual data sources such as webcams, cameras, video recorders, and scanners.

The machine vision process involves image detection and classification, image segmentation, object detection, face recognition or similarity learning, optical character recognition, motion tracking, image reconstruction, and image captioning, which describes an image using text.

Computer vision is being applied to automotive solutions, surveillance cameras, healthcare, biomedicine, and the market. Agricultural applications include vision in robots, sensors coupled in tractors, self-propelled machines, field machines (applied to fertilizer or pesticide), plant disease detection, weed identification, seed recognition, obstacle detection, fruit counting and picking, post-harvest machines (fruit selection), and quality of agricultural and food products.

New vision systems originating from sensors recently applied to research may constitute computer vision. This article includes technology such as multispectral and hyperspectral cameras, biospeckle, terahertz (THz) cameras, and recent research involving new sensors and new AI techniques. This review article addresses computer vision applications involving nondestructive techniques in determining quality parameters, mechanical properties, composition, appearance, identification of defects and grading of fruits and vegetables, three-dimensional (3D) reconstruction, plant disease detection for smart farming, and advanced quality control of fruit post-harvest.

An image is a two-dimensional (2D) matrix composed of pixels containing the element located in the image and surface information described by intensity values, for example, color and texture in the visible spectrum.

The image processing can vary, but some steps are similar: segmentation, feature extraction, and application of a dimension reduction method such as principal component analysis (PCA) to reduce classification information, and the employment of a classification technique. Classification techniques include statistical techniques (STs), neural networks (NNs), support vector machines (SVMs), and fuzzy logic (FL) (MAHENDRAN; AJAY VINO; ANANDAKUMAR, 2016MAHENDRAN, R.; AJAY VINO, S.; ANANDAKUMAR, S. Fundamentals of Computer Vision System for Sorting and Grading of Food Products. In: Reference Module in Food Science. [s.l.] Elsevier, 2016. p. B9780081005965031000.). Machine learning techniques, particularly deep learning, have been used with significant success in computer vision.

Furthermore, the recent improvements in deep learning, such as image classification, object detection, tracking, and image manipulation, enable new explorations of more complex and autonomous machine applications such as self-driving vehicles, humanoids, and drones.

Acquisition Systems

A typical digital image is obtained by recording radiant energy in the visible spectrum into a 2D array of numbers. An example of image formation is the conversion of visible light (absorbed, reflected, and scattered) into a camera’s electrical signals (ABDULLAH, 2016). Here, the acquisition system consists of an illumination source, a camera, a frame-grabber for analog-to-digital conversion, a computer, and a monitor to visualize the information.

Recently, the electromagnetic spectrum used in research has been expanded to increase the range of machine vision applications. Initially, only cameras in the visible light range were used; in recent times, research on camera systems that enable the observation of various parts of the electromagnetic spectrum has been conducted. Examples include camera systems such as computed tomography (CT), MRI, nuclear magnetic resonance (NMR), single-photon emission computed tomography (SPECT), positron emission tomography (PET), infrared and radio cameras (ABDULLAH, 2016), multispectral and hyperspectral cameras, biospeckle, and THz cameras.

Various cameras, ranging from the successful charge-coupled device (CCD) cameras to those using complementary metal–oxide–semiconductor (CMOS) technology, have been used. Using a single-chip CCD, monochrome imaging for sensing visible (Vis) or near-infrared (NIR) electromagnetic waves can be obtained. Color images can also be acquired using a single-chip CCD by modifying the CCD device’s pixels for red, green, and blue (RGB) color acquisition. A three-chip CCD camera can be used for color image acquisition (MAHENDRAN; AJAY VINO; ANANDAKUMAR, 2016MAHENDRAN, R.; AJAY VINO, S.; ANANDAKUMAR, S. Fundamentals of Computer Vision System for Sorting and Grading of Food Products. In: Reference Module in Food Science. [s.l.] Elsevier, 2016. p. B9780081005965031000.).

CCD cameras are widely employed in the analysis of food and agricultural products, facilitating the acquisition of exterior characteristics of objects such as color, shape, size, texture, and surface damage (MAHAJAN; DAS; SARDANA, 2015).

Visible and NIR spectroscopy enables the evaluation of the chemical composition and internal structure of agricultural products. These systems are frequently composed of an infrared source, a wavelength isolator, a detector, and a data processor. The most common sources are tungsten, halogen, and quartz–halogen lamps. Ren et al., (2020)REN, G. et al. Hyperspectral imaging for discrimination of Keemun black tea quality categories: Multivariate calibration analysis and data fusion. International Journal of Food Science & Technology, p. ijfs.14624, 2020. used a Vis-NIR spectrograph with wavelengths ranging from 350 to 1100 nm with a spectral resolution of 5 nm and two 150-W halogen lamps to acquire hyperspectral data of tea.

X-ray CT is formed using an X-ray tube, a beam collimator, and a detector. The images are formed after high-energy photon penetration and attenuation of X-ray radiation (CAKMAK, 2019CAKMAK, H. Assessment of fresh fruit and vegetable quality with non-destructive methods. In: Food Quality and Shelf Life. [s.l.] Elsevier, 2019. p. 303-331.).

The acquisition system for Raman spectroscopy is based on an excitation source at a wavelength range from visible to infrared, a wavelength separator, and a spotter as a CCD.

COMPUTER VISION TECHNOLOGIES

Color imaging

Advances in artificial vision enable us to obtain new knowledge and increase the efficiency and objectivity of inspection processes. This is because of the increase in the camera capabilities that enable obtaining higher resolution images (even in regions of the spectrum that are invisible to the human eye), a high capacity of computers to process data at high speed, and the evolution of system storage and communications. The automation level has increased exponentially in recent years, while equipment prices have decreased, enabling the creation of practical and complex applications, such as those related to the inspection of agricultural products (CUBERO et al., 2011CUBERO, S. et al. Advances in Machine Vision Applications for Automatic Inspection and Quality Evaluation of Fruits and Vegetables. Food and Bioprocess Technology, v. 4, n. 4, p. 487-504, 2011.).

Color cameras are the most widely used for computer vision because they capture images similar to those perceived by the human eye. The technology for acquiring these images is relatively inexpensive and very advanced, and some highly developed techniques to process information from these types of images exist. Color is an important quality characteristic for consumer acceptance, either aesthetic or linked to functional attributes and the stage of product development (PATHARE; OPARA; AL-SAID, 2013PATHARE, P. B.; OPARA, U. L.; AL-SAID, F. A. J. Colour Measurement and Analysis in Fresh and Processed Foods: A Review. Food and Bioprocess Technology, v. 6, n. 1, p. 36-60, 2013.). In nature, the perceived color is primarily determined by different types of pigments such as chlorophylls, carotenes, xanthophylls, and anthocyanins, which offer information on the type and state of the plants and their fruits (WALSH et al., 2020WALSH, K. B. et al. Visible-NIR ‘point’ spectroscopy in postharvest fruit and vegetable assessment: The science behind three decades of commercial use. Postharvest Biology and Technology, v. 168, p. 111246, 2020.). For example, color is used to estimate the ripeness or some internal quality parameters of fruits. Nevertheless, as this is a subjective human perception, tools to measure, quantify, and compare colors are required. These are color spaces that are mathematical models representing colors (DE-LA-TORRE et al., 2019DE-LA-TORRE, M. et al. Multivariate Analysis and Machine Learning for Ripeness Classification of Cape Gooseberry Fruits. Processes, v. 7, n. 12, p. 928, 2019.; PALLOTTINO et al., 2019PALLOTTINO, F. et al. Optoelectronic proximal sensing vehicle-mounted technologies in precision agriculture: A review. Computers and Electronics in Agriculture, v. 162, p. 859-873, 2019.). Frequently, the color space selected in digital images is RGB, which is native to cameras and computers. However, other color spaces, such as CIELAB or hue, saturation, and value (HSV), are also widely used as they attempt to represent human perception (DE-LA-TORRE et al., 2019DE-LA-TORRE, M. et al. Multivariate Analysis and Machine Learning for Ripeness Classification of Cape Gooseberry Fruits. Processes, v. 7, n. 12, p. 928, 2019.).

Le Nguyen et al. (2020)LE NGUYEN, L. P. et al. Application of hue spectra fingerprinting during cold storage and shelf-life of packaged sweet cherry. Journal of Food Measurement and Characterization, v. 14, n. 5, p. 2689-2702, 2020., measured the quality of sweet cherries by measuring the color of the surface as color is closely related to parameters such as anthocyanin concentration, sweetness, and fruit-specific flavor. The hue component was correlated with soluble solids content (SSC), firmness, respiration rate, and weight loss, achieving R2 values greater than 0.92 in all scenarios. The estimation of the internal quality of pomegranates using the color of the peel was investigated by Fashi et al. (2019)FASHI, M.; NADERLOO, L.; JAVADIKIA, H. The relationship between the appearance of pomegranate fruit and color and size of arils based on image processing. Postharvest Biology and Technology, v. 154, p. 52-57, 2019.. The aril color and size could be predicted with an R2 of 0.94 using artificial neural networks (ANNs). Huang et al. (2018)HUANG, X. et al. integration of computer vision and colorimetric sensor array for non-destructive detection of mango quality. Journal of Food Process Engineering, v. 41, 2018., evaluated the internal quality of mangoes by integrating textural information obtained using a CCD camera with color information provided by a colorimetric sensor array. The changes along the time of these parameters were related to hardness and total soluble solids (TSS) content. Color indices are some of the most commonly used tools to describe colors and quantify the color of fruits such as citrus fruits or tomatoes. Hadimani and Mittal (2019)HADIMANI, L.; MITTAL, N. Development of a computer vision system to estimate the colour indices of Kinnow mandarins. Journal of Food Science and Technology, v. 56, 2019. compared the traditional citrus color index (CUBERO et al., 2018CUBERO, S. et al. Application for the estimation of the standard citrus colour index (CCI) using image processing in mobile devices. Biosystems Engineering, v. 167, p. 63-74, 2018.; VIDAL et al., 2013VIDAL, A. et al. In-Line Estimation of the Standard Colour Index of Citrus Fruits Using a Computer Vision System Developed For a Mobile Platform. Food and Bioprocess Technology, v. 6, n. 12, p. 3412-3419, 2013.) with the CIELAB coordinates a* and b*, obtaining better results to describe the color of mandarin cv. “Kinnow” fruit. They also analyzed the relationship between the fruit’s exterior peel color and its internal characteristics. Bello et al. (2020)BELLO, T. B. et al. Tomato quality based on colorimetric characteristics of digital images. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 24, n. 8, p. 567-572, 2020., related color indices based on RGB coordinates to quality parameters of tomatoes and maturity stages. In a similar study, Costa et al. (2020)COSTA, A. G. et al. CLASSIFICATION OF ROBUSTA COFFEE FRUITS AT DIFFERENT MATURATION STAGES USING COLORIMETRIC CHARACTERISTICS. Engenharia Agrícola, v. 40, n. 4, p. 518-525, ago. 2020., combined color information in RGB, CIELAB, and HSV coordinates to predict the physicochemical quality properties of coffee fruits cv. “Robusta”. Cherry, immature, and over-ripe coffee fruits were correctly classified in 100% of the scenarios. One of the principal applications of color measurement is the estimation of the maturity stage. This property was determined by Santos Pereira et al. (2018)SANTOS PEREIRA, L. F. et al. Predicting the ripening of papaya fruit with digital imaging and random forests. Computers and Electronics in Agriculture, v. 145, p. 76-82, 2018. for papaya by analyzing twenty-one color features based on the RGB, CIELAB, and HSV color spaces.

When images are acquired, they are processed to obtain useful information. This task requires the development of efficient, robust, repeatable, rapid, and accurate processing algorithms. The analysis of these images provides information on the color, texture, or external properties as well as defects of objects. Among the essential steps of this process are segmentation, which consists of dividing the images into regions of interest (ROIs), and the extraction of characteristics to obtain the desired information from the regions or objects found (RUSS; NEAL, 2018RUSS, J. C.; NEAL, F. B. The Image Processing Handbook. [s.l.] CRC Press, 2018.). Segmentation can be performed using different approaches. Some are based on locating regions by searching textures, boundaries, or colors, while others classify individual pixels by attending some previous training.

Sharif et al. (2018)SHARIF, M. et al. Detection and classification of citrus diseases in agriculture based on optimized weighted segmentation and feature selection. Computers and Electronics in Agriculture, v. 150, p. 220-234, 2018., used a technique based on the multiclass SVM for citrus disease classification. After segmentation, color, textural, and geometric features were used to analyze images of oranges with a variety of peel defects. Color and textural features were also used by Zhang et al. (2020)ZHANG, C.; ZOU, K.; PAN, Y. A Method of Apple Image Segmentation Based on Color-Texture Fusion Feature and Machine Learning. Agronomy, v. 10, p. 972, 2020., to segment images of apple orchards and detected apples with similar colors to the leaves. Segmentation can be performed using a supervised method, with which the user must input some previous knowledge to the model, or an unsupervised method, with which no user intervention is required. Tian et al. (2019)TIAN, K. et al. Segmentation of tomato leaf images based on adaptive clustering number of K-means algorithm. Computers and Electronics in Agriculture, v. 165, p. 104962, 2019., developed an unsupervised segmentation method based on the k-means technique to segment diseased tomato plant leaves. Among the principal features observed in fruit inspection, those related to the quality perceived by consumers, such as size and color, have been the most studied. Liu et al. (2019a)LIU, L. et al. Design of a tomato classifier based on machine vision. PLOS ONE, v. 14, n. 7, p. e0219803, 2019a., designed a classifier based on computer vision to grade tomatoes based on their color, diameter, and shape using different image processing algorithms. Volume is not as used as a marketing decision but can be used as a weight estimator. The volume of mangoes was estimated by Mon and Zaraung (2020) from the length and obtained through the processing of 2D images. Morphological features from color images have also been extracted and used to individually detect kiwis arranged in clusters. Here calyx detection had an important function in separating and identifying individual fruits (FU et al., 2019FU, L. et al. A novel image processing algorithm to separate linearly clustered kiwifruits. Biosystems Engineering, v. 183, p. 184-195, 2019.).

Because of the ease of imitating the human eye, the development of rapid and efficient algorithms, and the processing power of computers, these systems have been used to analyze agricultural products on inspection lines in real-time, for instance, for mangoes (IBRAHIM et al., 2016IBRAHIM, M. F. et al. In-line sorting of harumanis mango based on external quality using visible imaging. Sensors (Switzerland), v. 16, n. 11, 2016.), peaches (LI et al., 2016LI, J. et al. Multispectral detection of skin defects of bi-colored peaches based on vis–NIR hyperspectral imaging. Postharvest Biology and Technology, v. 112, p. 121-133, 2016.), apples (UNAY et al., 2011UNAY, D. et al. Automatic grading of Bi-colored apples by multispectral machine vision. Computers and Electronics in Agriculture, v. 75, n. 1, p. 204-212, 2011.), mandarins (BLASCO et al., 2009bBLASCO, J. et al. Automatic sorting of satsuma (Citrus unshiu) segments using computer vision and morphological features. Computers and Electronics in Agriculture, v. 66, n. 1, p. 1-8, 2009b.) and pomegranate arils (BLASCO et al., 2009aBLASCO, J. et al. Development of a machine for the automatic sorting of pomegranate (Punica granatum) arils based on computer vision. Journal of Food Engineering, v. 90, n. 1, p. 27-34, 2009a.). In these electronic sorters, the fruit travels at a very high speed on a conveyor belt. When the fruit passes under a camera, several images are captured while the fruit rotates so that most of its surface is captured. All systems must be synchronized to capture the images in the exact moment and deliver the fruit by the outlet corresponding to the category decided by the inspection software (ALEIXOS et al., 2002ALEIXOS, N. et al. Multispectral inspection of citrus in real-time using machine vision and digital signal processors. Computers and Electronics in Agriculture, v. 33, n. 2, p. 121-137, 2002.).

Hyperspectral Systems

As stated earlier, systems based on color images are widely used in the industry to estimate the external characteristics of products. However, some internal damages or specific organoleptic characteristics are not visible and cannot be detected using traditional systems. Knowing the composition or internal properties of fruits or anticipating internal damage increases the added value and removes defective products from the production chain, increasing the batch’s overall quality. Properties such as soluble solids content, acidity, and texture are some of the parameters used to determine the maturity of fresh products. Among the optical detection technologies, hyperspectral imaging (HSI) has emerged as a potential tool for the non-destructive analysis of the internal quality and safety of agri-food products (LORENTE et al., 2012LORENTE, D. et al. Recent Advances and Applications of Hyperspectral Imaging for Fruit and Vegetable Quality Assessment. Food and Bioprocess Technology, v. 5, n. 4, p. 1121-1142, 2012.; LU et al., 2020LU, Y. et al. Hyperspectral imaging technology for quality and safety evaluation of horticultural products: A review and celebration of the past 20-year progress. Postharvest Biology and Technology, v. 170, p. 111318, 2020.). HSI combines the advantages of spectroscopy to capture chemical composition (CORTÉS et al., 2019CORTÉS, V. et al. Monitoring strategies for quality control of agricultural products using visible and near-infrared spectroscopy: A review. Trends in Food Science & Technology, v. 85, p. 138-148, 2019.; WALSH et al., 2020WALSH, K. B. et al. Visible-NIR ‘point’ spectroscopy in postharvest fruit and vegetable assessment: The science behind three decades of commercial use. Postharvest Biology and Technology, v. 168, p. 111246, 2020.) with the advantages of imaging to obtain spatial information (Figure 1) (JIA et al., 2020JIA, B. et al. Essential processing methods of hyperspectral images of agricultural and food products. Chemometrics and Intelligent Laboratory Systems, v. 198, p. 103936, 2020.).

The information captured by these systems is organized in a 3D matrix (known as a hypercube): 2D axes contain spectral information through the concepts of the line (X) and sample (Y) and the third dimension (λ) contains spectral information. Therefore, according to a specific pixel (x, y), its corresponding vector of spectral values can be obtained in the study’s wavelength range (LI et al., 2014LI, B. et al. Preliminary Research on Insect Damage Detection in Pecans Using Terahertz Spectroscopy. Spectroscopy and spectral analysis, v. 34, n. 05, p. 1196-1200, 2014.). Another critical advantage of HSI technology is its ability to acquire information from spectral regions that the human eye cannot see, such as ultraviolet, NIR, and infrared, generating specific fingerprints according to the composition or condition in evaluation (SIMKO; JIMENEZ-BERNI; FURBANK, 2015SIMKO, I.; JIMENEZ-BERNI, J.; FURBANK, R. Detection of decay in fresh-cut lettuce using hyperspectral imaging and chlorophyll fluorescence imaging. Postharvest Biology and Technology, v. 106, p. 44-52, 2015.).

The acquisition of the images is also slow, depending on the hardware used. Multispectral systems are more straightforward and faster implementations of hyperspectral systems in which a relatively lower number of bands are captured. Several technologies for capturing hyperspectral images exist. Among the most used systems are liquid crystal tunable filters (LCTFs) and image spectrophotometers (GÓMEZ-SANCHIS et al., 2014GÓMEZ-SANCHIS, J. et al. Development of a Hyperspectral Computer Vision System Based on Two Liquid Crystal Tuneable Filters for Fruit Inspection. Application to Detect Citrus Fruits Decay. Food and Bioprocess Technology, v. 7, n. 4, p. 1047-1056, 2014.). An LCTF is an electronically controlled optical filter that permits a selected wavelength to pass through and blocks others. Thus, images in the entire spectral range can be obtained by selecting different wavelengths. The main advantages of LCTF-based systems are their higher spatial resolution and image quality. In contrast, the process of image acquisition is slow, and spectral resolution is unsatisfactory. These systems were used by Munera et al. (2021)MUNERA, S. et al. Discrimination of common defects in loquat fruit cv. ‘Algerie’ using hyperspectral imaging and machine learning techniques. Postharvest Biology and Technology, v. 171, p. 111356, 2021., and Munera et al. (2019a)MUNERA, S. et al. Discrimination of astringent and deastringed hard ‘Rojo Brillante’ persimmon fruit using a sensory threshold by means of hyperspectral imaging. 2019a., to assess the internal quality of loquats and pomegranates, respectively. Thus, the internal components and some properties that are key to the fruit’s marketing can be estimated. The residual astringency of persimmon after a detergency treatment was determined by Munera et al. (2017, 2019b); to avoid possible fraud, Munera et al. (2018)MUNERA, S. et al. Potential of VIS-NIR hyperspectral imaging and chemometric methods to identify similar cultivars of nectarine. Food Control, v. 86, p. 1-10, 2018., discriminated externally identical varieties of nectarine but with different internal qualities. Citrus fruits, particularly the detection of non-visible rottenness caused by fungi (FOLCHFORTUNY et al., 2016), and the maturity of mangoes cv. “Manila” has also been investigated using these systems (VÉLEZ-RIVERA et al., 2014VÉLEZ-RIVERA, N. et al. Computer Vision System Applied to Classification of “Manila” Mangoes During Ripening Process. Food and Bioprocess Technology, 2014.). The combination of LCTF with structured-illumination reflectance imaging (SIRI) was created by Lu and Lu (2017)LU, Y.; LU, R. Development of a Multispectral Structured Illumination Reflectance Imaging (SIRI) System and Its Application to Bruise Detection of Apples. Transactions of the ASABE (American Society of Agricultural and Biological Engineers), v. 60, p. 1379-1389, 2017. to detect defects in apples.

Figure 1
Spatial and spectral information in a hyperspectral image

An LCTF system combined with a pushbroom imaging spectrometer was combined by Fan et al. (2018)FAN, S. et al. Data Fusion of Two Hyperspectral Imaging Systems with Complementary Spectral Sensing Ranges for Blueberry Bruising Detection. Sensors, v. 18, n. 12, p. 4463, 2018., to detect external damages on blueberries. Pushbroom imaging spectrophotometers acquire line-by-line spectral data and require the object to move beneath a camera while the image is being captured. In a camera with a matrix CCD sensor, while the camera captures the spatial information in one line of the CCD, the spectral information is projected onto the corresponding column. These systems are the most used, as they enable the capturing of moving objects, and therefore, in-line inspections. This system was used in the range 400–1000 and 900–1700 nm by Tsouvaltzis et al. (2020)TSOUVALTZIS, P. et al. Early detection of eggplant fruit stored at chilling temperature using different non-destructive optical techniques and supervised classification algorithms. Postharvest Biology and Technology, v. 159, p. 111001, 2020., to detect chilling injuries in eggplants. Fernandes et al. (2015)FERNANDES, A. et al. Brix, pH and anthocyanin content determination in whole Port wine grape berries by hyperspectral imaging and neural networks. Computers and Electronics in Agriculture, v. 115, p. 88-96, 2015., used an imaging spectrograph in the range of 380-1028 nm to determine anthocyanin content, sugar content, and acidity in grape berries. This technology has also been used to determine the internal quality of fruits such as oranges (AREDO et al., 2019AREDO, V. et al. Predicting of the Quality Attributes of Orange Fruit Using Hyperspec-tral Images. Journal of Food Quality and Hazards Control, 2019.), plum (LI et al., 2018LI, B. et al. Application of hyperspectral imaging for nondestructive measurement of plum quality attributes. Postharvest Biology and Technology, v. 141, p. 8-15, 2018.), banana (XIE; CHU; HE, 2018XIE, C.; CHU, B.; HE, Y. Prediction of banana color and firmness using a novel wavelengths selection method of hyperspectral imaging. Food Chemistry, v. 245, p. 132-140, 2018.), apples (TIAN et al., 2018TIAN, X. et al. A bi-layer model for non-destructive prediction of soluble solids content in apple based on reflectance spectra and peel pigments. Food Chemistry, v. 239, p. 1055-1063, 2018.), strawberries (WENG et al., 2020WENG, S. et al. Non-Destructive Detection of Strawberry Quality Using Multi-Features of Hyperspectral Imaging and Multivariate Methods. Sensors, v. 20, p. 3074, 2020.), pears (YU; LU; WU, 2018YU, X.; LU, H.; WU, D. Development of deep learning method for predicting firmness and soluble solid content of post-harvest Korla fragrant pear using Vis/NIR hyperspectral reflectance imaging. Postharvest Biology and Technology, v. 141, p. 39-49, 2018.), kiwi (HU; SUN; BLASCO, 2017HU, W.; SUN, D. W.; BLASCO, J. Rapid monitoring 1- MCP-induced modulation of sugars accumulation in ripening ‘Hayward’ kiwifruit by Vis/NIR hyperspectral imaging. Postharvest Biology and Technology, v. 125, p. 168-180, 2017.), tomato (VAN ROY et al., 2017VAN ROY, J. et al. Measuring colour of vine tomatoes using hyperspectral imaging. Postharvest Biology and Technology, v. 129, p. 79-89, 2017., 2018) and avocado (KÄMPER et al., 2020KÄMPER, W. et al. Rapid Determination of Nutrient Concentrations in Hass Avocado Fruit by Vis/NIR Hyperspectral Imaging of Flesh or Skin. Remote sensing, v. 12, p. 3409, 2020.).

However, because of the large amount of data generated by these systems and the relatively long acquisition time of hyperspectral images, these systems have not yet been implemented in the industry to conducted in-line controls of the quality of the products, although the first steps are already being conducted (VÁSQUEZ et al., 2018VÁSQUEZ, N. et al. Comparison between artificial neural network and partial least squares regression models for hardness modeling during the ripening process of Swiss-type cheese using spectral profiles. Journal of Food Engineering, v. 219, p. 8-15, 2018.).

NON-STANDARD TECHNIQUES OF COMPUTER VISION SYSTEMS

Biospeckle

Biospeckle is a non-invasive technique that is widely used to assess biological systems. This phenomenon is based on the interference of coherent electromagnetic waves after reflection from a surface, on which it occurs in a dynamic process. If this process occurs in a vegetal or animal tissue, the organelle size, cellular structure, cell growth, and division, biochemical reactions will affect the observed results.

There are several types of research with applications of biospeckle in different aspects of knowledge, such as obtaining information on the contamination of wastewater as an automatic analysis (VIANA; PIRES; BRAGA, 2017VIANA, D. C.; PIRES, J. F.; BRAGA, R. A. Biospeckle laser technique applied for estimating disinfection accomplishment of wastewaters subjected to chlorination. Process Safety and Environmental Protection, v. 109, p. 670-676, 2017.), characterizing plant tissue cultures (SCHOTT et al., 2020SCHOTT, C. et al. Biospeckle-characterization of hairy root cultures using laser speckle photometry. Engineering in Life Sciences, v. 20, n. 7, p. 287-295, 2020.); monitoring blood flow (ZHANG et al., 2019ZHANG, R. et al. Laser speckle imaging for blood flow based on pixel resolved zero-padding auto-correlation coefficient distribution. Optics Communications, v. 439, p. 38-46, 2019.), assessing seed quality (SINGH et al., 2020SINGH, P. et al. Application of laser biospeckle analysis for assessment of seed priming treatments. Computers and Electronics in Agriculture, v. 169, p. 105212, 2020.; VIVAS et al., 2017VIVAS, P. G. et al. Biospeckle activity in coffee seeds is associated non-destructively with seedling quality: Biospeckle activity in coffee seeds. Annals of Applied Biology, v. 170, n. 2, p. 141-149, mar. 2017.), evaluating the fermentation process (VIANA et al., 2017VIANA, D. C.; PIRES, J. F.; BRAGA, R. A. Biospeckle laser technique applied for estimating disinfection accomplishment of wastewaters subjected to chlorination. Process Safety and Environmental Protection, v. 109, p. 670-676, 2017.), and in applications ranging from the health field to agricultural products (AMARAL et al., 2017AMARAL, I. C. et al. Evaluation of the adsorption behavior of freeze-dried passion fruit pulp with added carriers by traditional biospeckle laser techniques. Drying Technology, v. 35, n. 1, p. 55-65, 2017.; HUMEAU-HEURTIER et al., 2012HUMEAU-HEURTIER, A. et al. Laser speckle contrast imaging: Multifractal analysis of data recorded in healthy subjects: Multifractal analysis of LSCI data. Medical Physics, v. 39, n. 10, p. 5849-5856, 2012.; YOUSSEF et al., 2019YOUSSEF, D. et al. Biospeckle local contrast analysis for surface roughness study of articular cartilage. Optik, v. 183, p. 55-64, 2019.).

Different image processing techniques are used to obtain information using biospeckle. Some algorithms return numerical results, such as the moment of inertia (MI) and absolute value difference (AVD) (ANSARI; NIRALA, 2016aANSARI, M. Z.; NIRALA, A. K. Biospeckle numerical assessment followed by speckle quality tests. Optik, v. 127, n. 15, p. 5825-5833, 2016a.; CARDOSO; BRAGA, 2014CARDOSO, R. R.; BRAGA, R. A. Enhancement of the robustness on dynamic speckle laser numerical analysis. Optics and Lasers in Engineering, v. 63, p. 19-24, 2014.). Graphical results are also obtained, for example, laser speckle contrast analysis (LASCA), motion history image (MHI), generalized difference, and Fujii (RABAL; BRAGA, 2009RABAL, H. J.; BRAGA, R. A. (EDS.). Dynamic laser speckle and applications. Boca Raton: CRC Press, 2009.).

This technique has been developed and combined with AI for applications in agriculture and postharvesting, such as the identification of chilling and freezing disorders in oranges; identification of bruising, maturation, and ripening in fruits and vegetables; and identification of defects and damages in fruits (MINZ; NIRALA, 2014MINZ, P. D.; NIRALA, A. K. Bio-activity assessment of fruits using Generalized Difference and Parameterized Fujii method. Optik, v. 125, n. 1, p. 314-317, 2014.; RAHMANIAN et al., 2020RAHMANIAN, A. et al. Application of biospeckle laser imaging for early detection of chilling and freezing disorders in orange. Postharvest Biology and Technology, v. 162, p. 111118, 2020.; WU; ZHU; REN, 2020WU, A.; ZHU, J.; REN, T. Detection of apple defect using laser-induced light backscattering imaging and convolutional neural network. Computers & Electrical Engineering, v. 81, p. 106454, 2020.).

Minz and Nirala (2014)MINZ, P. D.; NIRALA, A. K. Bio-activity assessment of fruits using Generalized Difference and Parameterized Fujii method. Optik, v. 125, n. 1, p. 314-317, 2014. used biospeckle to measure biological activity in apples, pears, and tomatoes, applying generalized difference and parameterized Fujii. Amaral et al. (2017)AMARAL, I. C. et al. Evaluation of the adsorption behavior of freeze-dried passion fruit pulp with added carriers by traditional biospeckle laser techniques. Drying Technology, v. 35, n. 1, p. 55-65, 2017., applied biospeckle to assess the sorption behavior of freeze-dried passion fruit. Wu et al. (2020)WU, A.; ZHU, J.; REN, T. Detection of apple defect using laser-induced light backscattering imaging and convolutional neural network. Computers & Electrical Engineering, v. 81, p. 106454, 2020., proposed a method for defect detection in apples based on laser backscattering imaging and convolutional neural networks (CNNs), and the method could effectively, non-destructively, and automatically identify the defect regions with a recognition rate of over 90%. Arefi et al. (2017)AREFI, A. et al. Towards real-time speckle image processing for mealiness assessment in apple fruit. International Journal of Food Properties, v. 20, n. sup3, p. S3135-S3148, 2017., used biospeckle combined with texture descriptors and ANNs to assess mealiness in apple fruits.

In biospeckle applications, the acquisition system is frequently composed of a laser source, lens, and CCD camera, which are considered simple and low-cost equipment (Figure 2).

The most commonly used laser is the He–Ne laser with a wavelength of 630-635 nm and low power (1-100 mW) (ANSARI et al., 2016ANSARI, M. Z. et al. Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image. Lasers in Medical Science, v. 31, n. 7, p. 1447-454, 2016.; ANSARI; NIRALA, 2016bANSARI, M. Z.; NIRALA, A. K. Following the drying process of Fevicol (adhesive) by dynamic speckle measurement. Journal of Optics, v. 45, n. 4, p. 357-363, 2016b.; CHATTERJEE; DISAWAL; PRAKASH, 2017aCHATTERJEE, A.; DISAWAL, R.; PRAKASH, S. Biospeckle Assessment of Bread Spoilage by Fungus Contamination Using Alternative Fujii Technique. In: BHATTACHARYA, I. et al. (Eds.). Advances in Optical Science and Engineering. Singapore: Springer Singapore, 2017a. v. 194, p. 395-401.; DENISOVA et al., 2013DENISOVA, YU. L. et al. Laser speckle technology in stomatology. diagnostics of stresses and strains of hard biotissues and orthodontic and orthopedic structures. Journal of Engineering Physics and Thermophysics, v. 86, n. 4, p. 940-951, 2013.; GAO; RAO, 2019GAO, Y.; RAO, X. Blackspot bruise in potatoes: susceptibility and biospeckle activity response analysis. Journal of Food Measurement and Characterization, v. 13, n. 1, p. 444-453, 2019.; GONZÁLEZPEÑA et al., 2016; KUMARI; NIRALA, 2019KUMARI, S.; NIRALA, A. K. Monitoring of functional blood flow on human hand due to effect of different treatments by laser biospeckle imaging. Lasers in Medical Science, v. 34, n. 6, p. 1167-1176, 2019.). Laser sources with wavelengths of 532 nm have also been reported in the literature (CHATTERJEE; DISAWAL; PRAKASH, 2017bCHATTERJEE, A.; DISAWAL, R.; PRAKASH, S. Evaluation of Aging Effect on Pea Seed Germination Using Generalized Difference Method. In: BHATTACHARYA, I. et al. (Eds.). Advances in Optical Science and Engineering. Springer Proceedings in Physics. Singapore: Springer Singapore, 2017b. v. 194, p. 403-408.; D’JONSILES et al., 2020D’JONSILES, M. F. et al. Optical study of laser biospeckle activity in leaves of Jatropha curcas L.: a non-invasive and indirect assessment of foliar endophyte colonization. Mycological Progress, v. 19, n. 4, p. 339-349, 2020.).

Figure 2
Biospeckle acquisition system

Hardware and software for biospeckle technology have been studied to improve image processing, portability of the equipment, and new techniques for obtaining information. Pieczywek et al. (2017)PIECZYWEK, P. M. et al. Exponentially smoothed Fujii index for online imaging of biospeckle spatial activity. Computers and Electronics in Agriculture, v. 142, p. 70-78, 2017., developed a method for the real-time evaluation of biospeckle using a live video stream with the Fujii method. Rivera and Braga Jr. (2020)RIVERA, F. P.; BRAGA, R. A. Illumination dependency in dynamic laser speckle analysis. Optics & Laser Technology, v. 128, p. 106221, 2020. compared biospeckle data for three different frequency bands of speckle signals and different light intensities. Catalano et al. (2019)CATALANO, M. D.; RIVERA, F. P.; BRAGA, R. A. Viability of biospeckle laser in mobile devices. Optik, v. 183, p. 897-905, 2019., performed image acquisition and created apps for image processing on a smartphone for biospeckle analysis. Rivera et al. (2019)RIVERA, F. P. et al. Sound as a qualitative index of speckle laser to monitor biological systems. Computers and Electronics in Agriculture, v. 158, p. 271-277, 2019., created a new method to obtain biospeckle information by employing sound to monitor biological systems.

Despite the wide range of research, Pandiselvam et al. (2020)PANDISELVAM, R. et al. Biospeckle laser technique – A novel non-destructive approach for food quality and safety detection. Trends in Food Science & Technology, v. 97, p. 1-13, 2020., indicated the challenges in using the biospeckle technique as the lack of a standard in applications and a requirement for commercial equipment for dedicated use; they also mentioned laser penetration, which cannot be used to assess the internal parts of agricultural products. Other limitations are the interference of light and sound vibration, which limit the use of the technique in the field.

Pieczywek et al. (2018), compared the biospeckle technique using visual inspection, hyperspectral imaging, and the chlorophyll fluorescence detection method in the early detection of bull’s eye rot in apples. They used three different laser wavelengths: 473, 532, and 830 nm. To obtain the information, they used the correlation coefficient, the Fuji index, the moment of inertia, and frequency analysis. Biospeckle exhibited a high level of performance in disease detection compared with hyperspectral imaging and chlorophyll fluorescence. They concluded that biospeckle has considerable potential as a diagnostic tool for detecting apple diseases at an early stage of their development. In comparison with visual inspection, hyperspectral imaging, and chlorophyll fluorescence, the authors indicated the advantages of biospeckle: a more straightforward experimental setup, low cost, and less time consuming with data processing.

Terahertz (THz) Image Systems

The emerging THz technology uses the energy present in the electromagnetic spectrum, from the relatively unexplored range from 100 GHz to 30 THz (LIN; SUN, 2020LIN, X.; SUN, D. W. Recent developments in vibrational spectroscopic techniques for tea quality and safety analyses. Trends in Food Science & Technology, v. 104, p. 163-176, 2020.; LIU et al., 2016LIU, W. et al. Application of terahertz spectroscopy imaging for discrimination of transgenic rice seeds with chemometrics. Food Chemistry, v. 210, p. 415-421, 2016.; WANG et al., 2019WANG, C. et al. Terahertz spectroscopic imaging with discriminant analysis for detecting foreign materials among sausages. Food Control, v. 97, p. 100-104, 2019.). It has attracted interest owing to the following characteristics: minor photon energy, deep penetration, and molecular resonance responses host ample physical and chemical information of biomolecular interactions; non-ionizing radiation; and the principle that different materials have different spectral fingerprints, which can be employed for identification, particularly for foods (JIANG; GE; ZHANG, 2020JIANG, Y.; GE, H.; ZHANG, Y. Quantitative analysis of wheat maltose by combined terahertz spectroscopy and imaging based on Boosting ensemble learning. Food Chemistry, v. 307, p. 125533, 2020.; OK et al., 2014OK, G. et al. Foreign-body detection in dry food using continuous sub-terahertz wave imaging. Food Control, v. 42, p. 284-289, 2014.; REN et al., 2019REN, A. et al. State-of-the-art in terahertz sensing for food and water security - A comprehensive review. Trends in Food Science & Technology, v. 85, p. 241-251, 2019.).

THz technology applications can be categorized into four main groups: sensing, imaging, spectroscopy, and communication, characterized by their non-destructive nature (REN et al., 2019REN, A. et al. State-of-the-art in terahertz sensing for food and water security - A comprehensive review. Trends in Food Science & Technology, v. 85, p. 241-251, 2019.). THz spectroscopy and imaging have had an increasing interest in the application for food quality and safety control, agricultural product analysis and quality inspection, and the inspection of stored food (LIU et al., 2016LIU, W. et al. Application of terahertz spectroscopy imaging for discrimination of transgenic rice seeds with chemometrics. Food Chemistry, v. 210, p. 415-421, 2016.; WANG; SUN; PU, 2017WANG, K.; SUN, D. W.; PU, H. Emerging non-destructive terahertz spectroscopic imaging technique: Principle and applications in the agri-food industry. Trends in Food Science & Technology, v. 67, p. 93–105, 2017.). However, owing to the low efficiency of THz energy sources and detectors and, consequently, the difficulty of building efficient instrumentation in this wavelength range, it was ignored until the mid-1990s (GOWEN; O’SULLIVAN; O’DONNELL, 2012GOWEN, A. A.; O’SULLIVAN, C.; O’DONNELL, C. P. Terahertz time domain spectroscopy and imaging: Emerging techniques for food process monitoring and quality control. Trends in Food Science & Technology, v. 25, n. 1, p. 40–46, 2012.).

THz technology can penetrate food materials deeper than other optical sources can and does not promote molecular motion such as rotation or vibration; similarly, it interacts weakly with nonpolar materials such as Teflon, polyethylene, and polytetrafluoroethylene. Both these properties make the use of THz waves a promising technology for noninvasive and non-destructive evaluation of food packaging and manufactured products (GOWEN; O’SULLIVAN; O’DONNELL, 2012GOWEN, A. A.; O’SULLIVAN, C.; O’DONNELL, C. P. Terahertz time domain spectroscopy and imaging: Emerging techniques for food process monitoring and quality control. Trends in Food Science & Technology, v. 25, n. 1, p. 40–46, 2012.; SHIN; CHOI; OK, 2018SHIN, H. J.; CHOI, S. W.; OK, G. Qualitative identification of food materials by complex refractive index mapping in the terahertz range. Food Chemistry, v. 245, p. 282–288, 2018.).

Equipment is the main obstacle for THz universalization as the cost of THz technology is higher than that of other imaging technologies such as hyperspectral (UV-Vis or NIR range), RGB cameras, and X-rays. However, the new developments in laser technologies, integrated optics, and its application in THz systems have made this technology more accessible for low-cost systems with high performance (REN et al., 2019REN, A. et al. State-of-the-art in terahertz sensing for food and water security - A comprehensive review. Trends in Food Science & Technology, v. 85, p. 241-251, 2019.; WANG; SUN; PU, 2017WANG, K.; SUN, D. W.; PU, H. Emerging non-destructive terahertz spectroscopic imaging technique: Principle and applications in the agri-food industry. Trends in Food Science & Technology, v. 67, p. 93–105, 2017.).

THz imaging systems using continuous waves or pulsed systems are acquired by rastering, moving the sample along the x and y dimensions, and recording the THz signal for each spatial position. The scheme of a THz image system is shown in Figure 3. Similarly, these can operate in the transmission or reflection modes and in the time or spatial domains. However, this operation is time-consuming depending on system characteristics and the desired spatial resolution (GOWEN; O’SULLIVAN; O’DONNELL, 2012GOWEN, A. A.; O’SULLIVAN, C.; O’DONNELL, C. P. Terahertz time domain spectroscopy and imaging: Emerging techniques for food process monitoring and quality control. Trends in Food Science & Technology, v. 25, n. 1, p. 40–46, 2012.; OK et al., 2019OK, G. et al. Large-scan-area sub-terahertz imaging system for non-destructive food quality inspection. Food Control, v. 96, p. 383-389, 2019.).

Different applications of THz image systems have been tested for food and agricultural product analysis, being used in the detection of foreign bodies (SHIN; CHOI; OK, 2018SHIN, H. J.; CHOI, S. W.; OK, G. Qualitative identification of food materials by complex refractive index mapping in the terahertz range. Food Chemistry, v. 245, p. 282–288, 2018.), determination of compound (JIANG; GE; ZHANG, 2020JIANG, Y.; GE, H.; ZHANG, Y. Quantitative analysis of wheat maltose by combined terahertz spectroscopy and imaging based on Boosting ensemble learning. Food Chemistry, v. 307, p. 125533, 2020.), pesticide, and antibiotic residues in agri-food products, characterization of edible oils and genetically modified food, etc. (WANG et al., 2019WANG, C. et al. Terahertz spectroscopic imaging with discriminant analysis for detecting foreign materials among sausages. Food Control, v. 97, p. 100-104, 2019.). Therefore, some studies on foodstuffs were conducted to test the water content of plant leaves (REN et al., 2019REN, A. et al. State-of-the-art in terahertz sensing for food and water security - A comprehensive review. Trends in Food Science & Technology, v. 85, p. 241-251, 2019.); the early detection of germinated wheat grains (JIANG et al., 2016JIANG, Y. et al. Early detection of germinated wheat grains using terahertz image and chemometrics. Scientific Reports, v. 6, n. 1, p. 21299, 2016.); detection of foreign bodies in noodle flour (LEE et al., 2012LEE, Y. K. et al. Detection of Foreign Bodies in Foods Using Continuous Wave Terahertz Imaging. Journal of Food Protection, v. 75, n. 1, p. 179-183, 2012.), chocolate (JÖRDENS; KOCH, 2008JÖRDENS, C.; KOCH, M. Detection of foreign bodies in chocolate with pulsed terahertz spectroscopy. Optical Engineering, v. 47, n. 3, p. 037003, 2008.), food powder (HERRMANN et al., 2002HERRMANN, M. et al. Terahertz imaging of objects in powders. IEE Proceedings - Optoelectronics, v. 149, n. 3, p. 116-120, 2002.), crackers and peanuts (HAN; PARK; CHUN, 2011HAN, S.; PARK, W. K.; CHUN, H. S. Development of Sub- THz gyrotron for real-time food inspection. 2011 International Conference on Infrared, Millimeter, and Terahertz Waves. Anais... In: 2011 INTERNATIONAL CONFERENCE ON INFRARED, MILLIMETER, AND TERAHERTZ WAVES, 2011.); damages in pecans (LI et al., 2014LI, B. et al. Preliminary Research on Insect Damage Detection in Pecans Using Terahertz Spectroscopy. Spectroscopy and spectral analysis, v. 34, n. 05, p. 1196-1200, 2014.), sugar, and milk (SHIN; CHOI; OK, 2018SHIN, H. J.; CHOI, S. W.; OK, G. Qualitative identification of food materials by complex refractive index mapping in the terahertz range. Food Chemistry, v. 245, p. 282–288, 2018.); detection of antibiotics in foodstuffs (REDOSANCHEZ et al., 2011), etc.

PROCESSING OF DATA FROM IMAGES

Ultimately, the spectral images are treated to obtain spectral signatures corresponding to vegetative states, the concentration of compounds, and the presence of microorganisms, etc. The extraction of these profiles from a specific area of the image utilizes a segmentation process or manual selection of the ROI.

Figure 3
Schematic of a THz imaging system in the reflection mode

The relationship between the obtained profiles and the quality parameters is analyzed to extract features from the profiles and develop prediction or classification models. These models, applied to new profiles, enable us to predict quality parameter conditions or obtain chemical images if they are applied to the entire image. The following subsections detail the main techniques used to model relationships.

Data exploration

Information content in images can be summarized into profiles, which commonly means a high number of variables per point, many of which are possibly non-relevant. Consequently, establishing if it will examine complete information or reduce non-relevant variables is necessary, reducing cost and time data analysis (OBLITAS et al., 2020OBLITAS, J. et al. The Use of Correlation, Association and Regression Techniques for Analyzing Processes and Food Products. Disponível em: <https://www.taylorfrancis.com/>. Acesso em: 1 nov. 2020.
https://www.taylorfrancis.com/...
).

Among the primary methods used, the PCA is the most common. It creates new variables (principal components) as a product between the eigenvector and the spectral vector, attempting to represent most of the variability in the data set using a small number of factors (JIANG; QIAO; HE, 2016JIANG, Y. et al. Early detection of germinated wheat grains using terahertz image and chemometrics. Scientific Reports, v. 6, n. 1, p. 21299, 2016.; MISHRA et al., 2019). Thus, several studies used PCA for different aims, such as the development of lightning correction in fruits (DONG et al., 2014DONG, C. et al. Detection of Thrips Defect on Green-Peel Citrus Using Hyperspectral Imaging Technology Combining PCA and B-Spline Lighting Correction Method. Journal of Integrative Agriculture, v. 13, n. 10, p. 2229-2235, 2014.).

Another group of importance in data exploration is those that perform variable selection as those grouped in cluster analysis (CA). Hierarchical cluster analysis (HCA) is one of the most used methods, and it explores the organization of variables into inter-groups and creates a hierarchy through dendrograms and nested cluster diagrams.

Finally, other less-used feature-extraction methods are competitive adaptive reweighted sampling (CARS) (FENG et al., 2019FENG, X. et al. Rapid detection of cadmium and its distribution in Miscanthus sacchariflorus based on visible and near-infrared hyperspectral imaging. Science of The Total Environment, v. 659, p. 1021-1031, 2019.; XIAO et al., 2020XIAO, K. et al. Prediction of soluble solid content of Agaricus bisporus during ultrasound-assisted osmotic dehydration based on hyperspectral imaging. LWT, v. 122, p. 109030, 2020.), spectral mixture analysis (SMA) (HARRIS; CHARNOCK; LUCAS, 2015HARRIS, A.; CHARNOCK, R.; LUCAS, R. M. Hyperspectral remote sensing of peatland floristic gradients. Remote Sensing of Environment, v. 162, p. 99-111, 2015.), mutual information feature selection (MIFS), max-relevance min-redundancy (MRMR), and sequential forward selection (SFS) (CEN et al., 2016CEN, H. et al. Non-destructive detection of chilling injury in cucumber fruit using hyperspectral imaging with feature selection and supervised classification. Postharvest Biology and Technology, v. 111, p. 352-361, 2016.), among others.

Classification techniques

Classification problems involve determining a mathematical model that can recognize samples belonging to specific classes. In images, the aim is to recognize objects or pixels with standard features and separate them into different classes, thereby segmenting the image.

According to Oblitas et al. (2020)OBLITAS, J. et al. The Use of Correlation, Association and Regression Techniques for Analyzing Processes and Food Products. Disponível em: <https://www.taylorfrancis.com/>. Acesso em: 1 nov. 2020.
https://www.taylorfrancis.com/...
, classification techniques can be grouped into three main categories: based on distance (of pixels with similar features), such as k-nearest neighbors (KNN); on probability (of a pixel belonging to any class), such as linear discriminant analysis (LDA), quadratic discriminant analysis (QDA), or unequal class models (UNEQ); and on experience (knowledge indicating a pixel belongs to any class), such as the ANN. Moreover, classification methods can be supervised when previous knowledge on the problem is supplied to the algorithm or unsupervised when the algorithm does not require any intervention. Most classification algorithms used in agri-food inspection are supervised because they must involve previous training steps. These are rapid, but because of the vast variability present in these products, they require frequent retraining.

According to Cai et al. (2018)CAI, W. et al. Network linear discriminant analysis. Computational Statistics & Data Analysis, v. 117, p. 32-44, 2018., LDA is one of the most popular supervised methods for food analysis; it estimates the multivariable probability density functions for each class. LDA begins with the estimation of the location and dispersion parameters. It was applied in laboratory studies such as that developed by Rahman et al. (2018)RAHMAN, A. et al. Hyperspectral imaging technique to evaluate the firmness and the sweetness index of tomatoes. Korean Journal of Agricultural Science, v. 45, n. 4, p. 823-837, 2018., on vegetable tissue micrograph for microstructure classification, Shafiee et al. (2016)SHAFIEE, S. et al. Detection of Honey Adulteration using Hyperspectral Imaging. IFAC-PapersOnLine, 5th IFAC Conference on Sensing, Control and Automation Technologies for Agriculture AGRICONTROL 2016. v. 49, n. 16, p. 311-314, 2016., on determining honey adulteration, comparing different classifiers and obtaining LDA accuracy over 90%, or in remote sensing such as in the study of Furlanetto et al. (2020)FURLANETTO, R. H. et al. Hyperspectral reflectance imaging to classify lettuce varieties by optimum selected wavelengths and linear discriminant analysis. Remote Sensing Applications: Society and Environment, v. 20, p. 100400, 2020. for vegetable identification.

Based on variable selection, different techniques can be applied, such as partial least squares–discriminant analysis (PLSR-DA) for object classification in hyperspectral images (ZHANG et al., 2018ZHANG, L. et al. Salient object detection in hyperspectral imagery using multi-scale spectral-spatial gradient. Neurocomputing, v. 291, p. 215-225, 2018.) and discrimination of polyethylene films (BONIFAZI; CAPOBIANCO; SERRANTI, 2018BONIFAZI, G.; CAPOBIANCO, G.; SERRANTI, S. A hierarchical classification approach for recognition of low-density (LDPE) and high-density polyethylene (HDPE) in mixed plastic waste based on short-wave infrared (SWIR) hyperspectral imaging. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 198, p. 115-122, 2018.).

Two techniques, specifically used in classification problems, are SVMs and ANNs, both of which are widely used in the field of pattern recognition for linear and non-linear classification scenarios (LI et al., 2020LI, M. et al. Pickled and dried mustard foreign matter detection using multispectral imaging system based on single shot method. Journal of Food Engineering, v. 285, p. 110106, 2020.). Their applications involve images in the RGB format (CASTRO et al., 2019CASTRO, W. et al. Classification of Cape Gooseberry Fruit According to its Level of Ripeness Using Machine Learning Techniques and Different Color Spaces - IEEE Journals & Magazine. IEEE access, v. 7, p. 27389-27400, 2019.; JIANG et al., 2020aJIANG, F. et al. Image recognition of four rice leaf diseases based on deep learning and support vector machine. Computers and Electronics in Agriculture, v. 179, p. 105824, 2020a.), multispectral imaging (YUet al., 2020YU, P. et al. Rapid detection of moisture content and shrinkage ratio of dried carrot slices by using a multispectral imaging system. Infrared Physics & Technology, v. 108, p. 103361, 2020.), and hyperspectral imaging (SHAFIEE et al., 2016SHAFIEE, S. et al. Detection of Honey Adulteration using Hyperspectral Imaging. IFAC-PapersOnLine, 5th IFAC Conference on Sensing, Control and Automation Technologies for Agriculture AGRICONTROL 2016. v. 49, n. 16, p. 311-314, 2016.).

Kang et al. (2020)KANG, R.; PARK, B.; CHEN, K. Identifying non-O157 Shiga toxin-producing Escherichia coli (STEC) using deep learning methods with hyperspectral microscope images. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 224, p. 117386, 2020., compared LDA, SVMs, and softmax regression to classify serogroups of Escherichia coli. Liu et al. (2019b)LIU, W. et al. Rapid determination of aflatoxin B1 concentration in soybean oil using terahertz spectroscopy with chemometric methods - ScienceDirect. Food Chemistry, v. 293, p. 213-219, 2019b., evaluated least-squares support vector machines (LS-SVMs), backpropagation neural network (BPNNs), and random forest (RF) to predict the content of aflatoxin in soybean oil.

Regression techniques

In food engineering, another type of analysis is related to the prediction of the concentration of compounds of interest in foods; regression methods are used for these tasks. Similarly, for classification, their behavior can be linear or non-linear, and the models must manage this characteristic (OBLITAS et al., 2020OBLITAS, J. et al. The Use of Correlation, Association and Regression Techniques for Analyzing Processes and Food Products. Disponível em: <https://www.taylorfrancis.com/>. Acesso em: 1 nov. 2020.
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).

Some commonly used methods are linear regression (LR) and multilinear regression (MLR), which uses predictor variables without transformation, principal component regression (PCR), and partial least square regression (PLSR), which uses a previous transformation of variables into its principal components.

PLSR is one of the widely used chemometric techniques, for instance, to extract data from hyperspectral images, owing to its capacity to reduce dimensionality in complex systems (JIA et al., 2020JIA, B. et al. Essential processing methods of hyperspectral images of agricultural and food products. Chemometrics and Intelligent Laboratory Systems, v. 198, p. 103936, 2020.). The equation for this technique can be summarized as Y = βX + e, where Y is the matrix of the predicting variable, β is the matrix of beta coefficients, X is the measured variable, and e is the model error (VÁSQUEZ et al., 2018VÁSQUEZ, N. et al. Comparison between artificial neural network and partial least squares regression models for hardness modeling during the ripening process of Swiss-type cheese using spectral profiles. Journal of Food Engineering, v. 219, p. 8-15, 2018.). Its applications have ranged over a variety of image types such as multispectral satellite images (MALLAH NOWKANDEH; NOROOZI; HOMAEE, 2018MALLAH NOWKANDEH, S.; NOROOZI, A. A.; HOMAEE, MEHDI. Estimating soil organic matter content from Hyperion reflectance images using PLSR, PCR, MinR and SWR models in semi-arid regions of Iran. Environmental Development, v. 25, p. 23-32, 2018.), multispectral images of unmanned vehicles (GUO et al., 2020), laboratory multispectral imaging (YU et al., 2020YU, P. et al. Rapid detection of moisture content and shrinkage ratio of dried carrot slices by using a multispectral imaging system. Infrared Physics & Technology, v. 108, p. 103361, 2020.), hyperspectral images (XU et al., 2021XU, S. et al. Integrating hyperspectral imaging with machine learning techniques for the high-resolution mapping of soil nitrogen fractions in soil profiles. Science of The Total Environment, v. 754, p. 142135, 2021.), and thermal images (ELSAYED et al., 2017ELSAYED, S. et al. Thermal imaging and passive reflectance sensing to estimate the water status and grain yield of wheat under different irrigation regimes. Agricultural Water Management, v. 189, p. 98-110, 2017.).

Although PLSR models can reduce dimensionality based on B-values, thus reducing the effect of nonrelevant variables (KIALA; ODINDI; MUTANGA, 2017KIALA, Z.; ODINDI, J. O. J.; MUTANGA, O. Potential of interval partial least square regression in estimating leaf area index. South African Journal of Science, v. 113, n. 9/10, p. 9-9, 2017.), some authors reported that PLSR models can be improved. Procedures include removing some intervals, using variants such as the interval PLSR (iPLSR) (CHRISTENSEN et al., 2017CHRISTENSEN, J. et al. Rapid Spectroscopic Analysis of Marzipan-Comparative Instrumentation: Journal of Near Infrared Spectroscopy, 2017.), moving window PLSR (mwPLSR) (RONGTONG et al., 2018RONGTONG, B. et al. Determination of water activity, total soluble solids and moisture, sucrose, glucose and fructose contents in osmotically dehydrated papaya using near-infrared spectroscopy. Agriculture and Natural Resources, v. 52, n. 6, p. 557-564, 2018.), searching combination moving window PLSR (RONGTONG et al., 2018RONGTONG, B. et al. Determination of water activity, total soluble solids and moisture, sucrose, glucose and fructose contents in osmotically dehydrated papaya using near-infrared spectroscopy. Agriculture and Natural Resources, v. 52, n. 6, p. 557-564, 2018.), synergy interval PLSR (SyPLSR) (GUAN et al., 2019GUAN, X. et al. Evaluation of moisture content in processed apple chips using NIRS and wavelength selection techniques. Infrared Physics & Technology, v. 98, p. 305–310, 2019.), genetic algorithm PLSR (gaPLSR) (GUAN et al., 2019GUAN, X. et al. Evaluation of moisture content in processed apple chips using NIRS and wavelength selection techniques. Infrared Physics & Technology, v. 98, p. 305–310, 2019.), and recursive PLSR (rPLSR) (ISLAM et al., 2018ISLAM, MD. N. et al. Non-invasive Determination of Firmness and Dry Matter Content of Stored Onion Bulbs Using Shortwave Infrared Imaging with Whole Spectra and Selected Wavelengths. Applied Spectroscopy, v. 72, n. 10, p. 1467-1478, 2018.).

Advanced techniques (Machine learning)

Machine learning is a subdivision of computer science applied for pattern recognition and computational learning in AI (SWAMYNATHAN, 2019SWAMYNATHAN, M. Mastering Machine Learning with Python in Six Steps: A Practical Implementation Guide to Predictive Data Analytics Using Python. Berkeley, CA: Apress, 2019.). It is based on the training of neural networks that enables machines to learn from a database and make predictions. The main advantage of this learning is improved performance, as it is exposed to new and larger databases. The categories of machine learning are supervised, unsupervised, and reinforcement learning.

Deep learning is a subfield of machine learning whose algorithms aim to bring machine intelligence closer to the human level, making them capable of solving any problem in a specific subject. Deep learning has been applied successfully to solve computer vision, audio processing, and text mining problems (SWAMYNATHAN, 2019SWAMYNATHAN, M. Mastering Machine Learning with Python in Six Steps: A Practical Implementation Guide to Predictive Data Analytics Using Python. Berkeley, CA: Apress, 2019.). Examples of deep learning are CNNs, which are advantageously applied in image classification.

Applications based on deep learning have increased over the last decade owing to the significant advances in AI and the increase in computing power since the arrival of graphic processor units). These advances have been motivated by two reasons: the increase in available data (the famous Big Data) and the application of machine learning methods that are key to companies such as Facebook, Google, or LinkedIn. In recent years, a revolution in machine learning with the emergence of deep learning algorithms has occurred. Among them, deep convolutional neural networks (DCNNs) are currently the state of the art in computer vision applications. Until the emergence of deep neuronal models, multilayer neuronal models with more than two hidden layers were considered useless. In the 2000s, no significant research was conducted that used more than two hidden layers. These models had two main problems: a) the initialization of the parameters and b) overfitting. Therefore, the fruit inspection systems that have been developed using these techniques have not been implemented.

Currently, the emergence of deep multilayer and DCNN models has solved these problems. DCNNs are flexible algorithms that have been used successfully in the inspection problems of processed foods (KATO et al., 2019KATO, S. et al. Snack Texture Estimation System Using a Simple Equipment and Neural Network Model. Future Internet, v. 11, n. 3, p. 68, 2019.) or fresh fruit (ASHRAF et al., 2019ASHRAF, S. et al. Fruit Image Classification Using Convolutional Neural Networks: International Journal of Software Innovation, v. 7, n. 4, p. 51-70, 2019.; GIEFER et al., 2019GIEFER, L. A. et al. Deep Learning-Based Pose Estimation of Apples for Inspection in Logistic Centers Using Single- Perspective Imaging. Processes, v. 7, n. 7, p. 424, 2019.; STEINBRENER; POSCH; LEITNER, 2019STEINBRENER, J.; POSCH, K.; LEITNER, R. Hyperspectral fruit and vegetable classification using convolutional neural networks. Computers and Electronics in Agriculture, v. 162, p. 364-372, 2019.). All references found are from 2018, which indicates the novelty of the techniques. However, examples of the use of DCNN in fresh fruit inspection using hyperspectral images have not been found in the literature. This is because the acquisition and labeling of images by an expert can be very tedious. Algorithms based on deep learning require many images for training. Its depth implies many parameters, and, as with other models, this fact involves many labeled samples; thus, the depth is increased by the variability of fruits.

COMPUTER VISION APPLICATIONS IN FOOD AND AGRICULTURAL PRODUCTS

Digital Image Systems applied to Agriculture 4.0

Recent studies presented digital images coupled with machines and unmanned aerial vehicle (UAV) devices to monitor crops in the field, recognize crop productivity, localize robotics, and enable the sustainable use of natural resources such as water, agrochemicals, and fertilizers.

Andújar et al. (2019)ANDÚJAR, D. et al. Aerial imagery or on-ground detection? An economic analysis for vineyard crops. Computers and Electronics in Agriculture, v. 157, p. 351-358, 2019., compared aerial imagery with on-ground detection using an RGB-depth camera and Microsoft Kinect v.2, and they observed that UAVs are affordable and can encompass a larger surface area for vineyards.

Abdelghafour et al. (2019)ABDELGHAFOUR, F. et al. A Bayesian framework for joint structure and colour based pixel-wise classification of grapevine proximal images. Computers and Electronics in Agriculture, v. 158, p. 345-357, mar. 2019., applied a proximal image to describe the canopy structure of plants for precision viticulture. A foreground extraction was performed based on color information, pixel-wise feature extraction with texture captured with local structure tensor, pixel-wise classification, and spatial regularization. This system with optical sensors enables the analysis of agronomic data as an automated and non-intrusive technique. Furthermore, the information obtained on plant productivity is essential for precision fertilization and irrigation.

Pinto de Aguiar et al. (2020)PINTO DE AGUIAR, A. S. et al. Vineyard trunk detection using deep learning - An experimental device benchmark. Computers and Electronics in Agriculture, v. 175, p. 105535, 2020., utilized feature extraction on vineyards for robotics localization and mapping to locate vine trunks on images. They used low-power and low-cost equipment such as Google’s USB Accelerator and NVIDIA’s Jetson Nano. Google’s USB Accelerator is adaptable with TensorFlow Lite, used in mobile and portable equipment, and can perform image classification, object detection, and semantic segmentation. A small version of You Only Look Once (YOLO) was used to identify vine trunks in real-time. YOLO is used to detect objects in full images and, when applied to a webcam, can detect moving objects (REDMON et al., 2016).

Plant Disease Detection for Smart Farming

The integration of digital images, several sensors, the Internet of Things, deep learning, robust algorithms, UAVs, and smartphones is increasingly enabling the detection of diseases in plants in the field. The inclusion of different types of data enables smarter systems to perform field operations.

Ashok et al. (2020)ASHOK, S. et al.Tomato Leaf Disease Detection Using Deep Learning Techniques. 2020 5th International Conference on Communication and Electronics Systems (ICCES). Anais... In: 2020 5TH INTERNATIONAL CONFERENCE ON COMMUNICATION AND ELECTRONICS SYSTEMS (ICCES). COIMBATORE, India: IEEE, jun. 2020Disponível em: <https://ieeexplore.ieee.org/document/9137986/>. Acesso em: 28 out. 2020
https://ieeexplore.ieee.org/document/913...
, proposed a 98% accuracy method to detect disorders in tomato plant leaves through image processing. Detecting plant leaf diseases in advance is essential to leveraging production and avoiding crop losses. The initial step was pre-processing using a Gaussian filter, and then feature extraction using the discrete wavelet transform with the use of coefficients with sub-bands and the grey level co-occurrence matrix (GLCM) computed correlation. A CNN algorithm was used to extract features that mapped the pixel values and evaluated it using the trained dataset image.

Kulkarni (2018) proposed training a CNN model to identify the type of crop and detect diseases in a public dataset composed of normal and damaged crop leaves. MobileNet and InceptionV3 models were used, and accuracies of 99.62% and 99.74% for crop type and 99.04% and 99.45% accuracy for crop disease were obtained, respectively.

Militante et al. (2019)MILITANTE, S. V.; GERARDO, B. D.; MEDINA, R. P. Sugarcane Disease Recognition using Deep Learning. 2019 IEEE Eurasia Conference on IOT, Communication and Engineering (ECICE). Anais... In: 2019 IEEE EURASIA CONFERENCE ON IOT, COMMUNICATION AND ENGINEERING (ECICE). Yunlin, Taiwan: IEEE, out. 2019Disponível em: <https://ieeexplore.ieee.org/ document/8942690/>. Acesso em: 28 out. 2020.
https://ieeexplore.ieee.org/ document/89...
, used deep learning techniques to identify and recognize sugarcane diseases. The study consisted of training and testing a deep learning model, including a 13.842 sugarcane image dataset of disease-infected and healthy leaves. The model could detect healthy and unhealthy leaves, classify diseased leaves, and achieve a 95% accuracy with 60 epochs. The methodology consisted of capturing an image dataset using a camera, pre-processing the images, obtaining features from the resized images, and using fully connected layers for classification; for feature extraction, it used convolutional and pooling layers. The main advantage of these techniques is the ability to extract information from large amounts of heterogeneous data. Thus, they are useful for processing hyperspectral images. In this context, Polder et al. (2019)POLDER, G. et al. Potato Virus Y Detection in Seed Potatoes Using Deep Learning on Hyperspectral Images. Frontiers in Plant Science, v. 10, p. 209, 2019., used fully convolutional networks (FCNs) to detect potato virus Y (PVY) in the field. They arranged the camera in a measurement box installed in front of a tractor that drove through row potato fields at a constant speed to capture images. The FCN performed well in predicting the PVY-infected plants despite limited training data. The detection of infected plants was between 75% and 92% (recall values).

Castelao Tetila et al. (2017)CASTELAO TETILA, E. et al. Identification of Soybean Foliar Diseases Using Unmanned Aerial Vehicle Images. IEEE Geoscience and Remote Sensing Letters, v. 14, n. 12, p. 2190-2194, 2017., proposed a system using simple linear iterative clustering for segmentation to identify plant leaves and describe the features of foliar characteristics, including color, texture, shape, and gradient via UAV images.

Using highly robust algorithms, Zhao et al. (2020)ZHAO, Y. et al. An effective automatic system deployed in agricultural Internet of Things using Multi-Context Fusion Network towards crop disease recognition in the wild. Applied Soft Computing, v. 89, p. 106128, 2020., solved the automatic identification of crop diseases using images obtained from the field using deep learning. They used the Internet of Things to collect contextual information as useful features in a modern recognition system to identify crop diseases. Contextual features such as the season, geographic location, temperature, and humidity, were fused with visual features in a state-of-the-art crop disease recognition method.

For automatic tractor piloting, a control system based on binocular vision was developed. This system enabled the machine to identify the path and was an excellent in-field operation. Zhang et al. (2018)ZHANG, L. et al. Salient object detection in hyperspectral imagery using multi-scale spectral-spatial gradient. Neurocomputing, v. 291, p. 215-225, 2018., applied this system to cotton field management.

To reduce chemical inputs in vineyard crops, Kerkech et al. (2020)KERKECH, M.; HAFIANE, A.; CANALS, R. Vine disease detection in UAV multispectral images using optimized image registration and deep learning segmentation approach. Computers and Electronics in Agriculture, v. 174, p. 105446, 2020., created a method for disease detection in the vine field, applying deep learning on UAV images. The method used two sensors and combined visible and infrared images. This information was inputted into a fully convolutional neural network to allocate each pixel into shadow, ground, healthy, and symptom. As a result, the technique obtained over 92% of disease detection in grapevines and 87% in leaves, which is a promising application for computer vision.

Rahaman et al. (2019)RAHAMAN, D. M. M. et al. Grapevine Nutritional Disorder Detection Using Image Processing. In: LEE, C.; SU, Z.; SUGIMOTO, A. (Eds.). Image and Video Technology. Lecture Notes in Computer Science. Cham: Springer International Publishing, 2019. v. 11854p. 184-196., created a smartphone app to obtain and process images of grapevines to detect nutritional disorders using an SVM.

3D Reconstruction

The 3D reconstruction of agricultural products and food has facilitated the automation and application of autonomous machinery activities in the field. With this technique, reconstructing, identifying, and estimating the volume of fruits, parts of plants, weeds, insects, and pests is possible. Robots and agricultural machines can also identify paths and obstacles in the field.

In robotics, the most commonly used methods to convert distances into 3D points are the time-of-flight systems and triangulation techniques. The time of flight measures when a signal reaches a surface and returns to the emitter and an example is the laser measurement system. In triangulation techniques, the distances are estimated by attaching parts of a scene with two different views. The two views must be calibrated to determine the distance (LIU; LEE; CHAHL, 2017LIU, H.; LEE, S. H.; CHAHL, J. S. A Multispectral 3-D Vision System for Invertebrate Detection on Crops. IEEE Sensors Journal, v. 17, n. 22, p. 7502-7515, 2017.).

Algorithms that describe 3D features can be divided into two classes: global feature-based and local feature-based. The first uses a set of features with the geometric properties of an integral 3D object. The second uses features with characteristics of the local region points. The local feature-based method can use a local reference frame. It can also use a histogram or statistics of a normal or curvature to model a feature descriptor (LIU; LEE; CHAHL, 2017LIU, H.; LEE, S. H.; CHAHL, J. S. A Multispectral 3-D Vision System for Invertebrate Detection on Crops. IEEE Sensors Journal, v. 17, n. 22, p. 7502-7515, 2017.).

Gao et al. (2019)GAO, J. et al. Three Dimensional Reconstruction of Watermelon for Multimedia Traceability System. ETP International Journal of Food Engineering, p. 1-8, 2019., studied 3D reconstruction of watermelon using a multimedia traceability system. Using sequential pictures captured around a watermelon from different angles, they matched feature points using a scaleinvariant feature transform algorithm to generate a sparse and dense point cloud using the motion method and multi-view stereo method, respectively. Texture mapping and model meshing were conducted using the Poisson surface reconstruction approach. The 3D reconstruction provided parameters such as shape, color, texture, geometric size, and volume. This experiment demonstrated that 3D reconstruction can be used to calculate size and volume with a relative error of approximately 1%, indicating that the volume measurement can be used to detect fruits with atypical densities and pull them out from the traceability system in the production line.

Tao and Zhou (2017)TAO, Y.; ZHOU, J. Automatic apple recognition based on the fusion of color and 3D feature for robotic fruit picking. Computers and Electronics in Agriculture, v. 142, p. 388-396, nov. 2017. created an automatic system for robot perception in the 3D space, giving trajectory calculation and strategic planning to pick only the fruit in the field.

Determination of Quality Parameters: Mechanical Properties, Composition, and Appearance

Computer vision systems have been used over the last two decades in several studies to predict quality parameters and, based on this, to classify foodstuffs for collecting, processing, and storage (LU et al., 2020LU, Y. et al. Hyperspectral imaging technology for quality and safety evaluation of horticultural products: A review and celebration of the past 20-year progress. Postharvest Biology and Technology, v. 170, p. 111318, 2020.). The main quality parameters for agri-food products include flavor, TSS, titratable acidity, sugar content, color, appearance, and firmness. To assess these parameters, a variety of systems have been used as traditional RGB images, multispectral, hyperspectral, terahertz, Raman images, or those that produce intensity images such as fluorescence images, laser-light backscattering, and X-ray images. Table 1 summarizes some of the recent works related to the quality parameters of food, using different non-destructive optical technologies.

Cakmak (2019)CAKMAK, H. Assessment of fresh fruit and vegetable quality with non-destructive methods. In: Food Quality and Shelf Life. [s.l.] Elsevier, 2019. p. 303-331. presented a review of nondestructive techniques for the quality assessment of agricultural products. They argued that Raman and surface-enhanced Raman spectroscopy (SERS) is an essential technique for evaluating agri-food chemical properties. MSI, HSI system, and NIR spectroscopy determine TSS, moisture content, titratable acidity, sugar content, and firmness of fruits and vegetables. Moreover, NIR spectroscopy has also been used in in-field and portable equipment.

Table 1
Computer vision systems and their applications in the inspection of food quality

Identification of Defects in Fruits and Vegetables

The primary aim of detecting defects in vegetables is to provide high-quality products for the customer and ensure reasonable prices for the market. The most frequently found defects are mechanical damage, morphological disorders, internal defects, pathological disorders, and physiological disorders, which may be visible or latent and internal (NTURAMBIRWE; OPARA, 2020NTURAMBIRWE, J. F. I.; OPARA, U. L. Machine learning applications to non-destructive defect detection in horticultural products. Biosystems Engineering, v. 189, p. 60-83, 2020.).

Bruising is a typical damage that occurs during harvest and post-harvest manipulation. Its detection in fruits is primarily performed using manual inspection, which is time-consuming and mistake-prone. Traditional computer vision has been used for bruise detection, but with limited applications. To increase computer vision capacity to identify bruises in vegetables, Du et al. (2020)DU, Z. et al. Recent advances in imaging techniques for bruise detection in fruits and vegetables. Trends in Food Science & Technology, v. 99, p. 133-141, 2020., proposed combining new imaging techniques, such as biospeckle, fluorescence imaging, structural illumination reflectance imaging, hyperspectral/multispectral imaging, X-ray imaging, MRI, and thermal imaging with computer vision. The vision system can also incorporate deep learning methods, ANNs, and CNNs. For future research, the authors proposed that studies should focus on reducing equipment cost and miniaturization.

Some essential and recent research papers on the identification of fruit damage are described below.

Andrushia and Trephena (2019) created a computer vision technique to automatically diagnose surface diseases on mango fruits, adopting an artificial-bee- colony-optimized feature set. The processing phases consist of removing the background, extracting the color, shape, and texture features, a metaheuristic approach to select the features, and the classification into good and diseased fruits.

Marino et al. (2020)MARINO, S.; BEAUSEROY, P.; SMOLARZ, A. Unsupervised adversarial deep domain adaptation method for potato defects classification. Computers and Electronics in Agriculture, v. 174, p. 105501, 2020., proposed potato defect classification using an unsupervised deep-domain-adaptation method based on adversarial training.

Chithra and Henila (2020)CHITHRA, PL.; HENILA, M. Apple fruit sorting using novel thresholding and area calculation algorithms. Soft Computing, 2020. proposed a new algorithm to obtain images of the defective area of apple fruits in the sorting task. This task is essential to increase the speed and quantity of the sorting process, aiding the farmers to separate healthy fruit accurately and reduce costs in post-harvest operations. The image processing included rapid global thresholding, a discrete wavelet transformation to obtain statistical and texture features, a naive Bayesian classification model, a k-means clustering to segment the damaged area, and an algorithm to calculate the area and perform sorting decisions.

Athiraja and Vijayakumar (2020)ATHIRAJA, A.; VIJAYAKUMAR, P. Banana disease diagnosis using computer vision and machine learning methods. Journal of Ambient Intelligence and Humanized Computing, 2020. identified banana diseases at a much earlier stage using computer vision and machine learning. They performed pre-processing techniques and image standardization; color, shape, and texture features were used for feature extraction; finally, they used classification techniques.

Nturambirwe and Opara (2020)NTURAMBIRWE, J. F. I.; OPARA, U. L. Machine learning applications to non-destructive defect detection in horticultural products. Biosystems Engineering, v. 189, p. 60-83, 2020. presented a review of the novel machine learning approaches applied to diverse sensors to identify damages to agricultural products. They argued that despite the high potential of vision systems to detect internal and external defects in horticultural products, some limitations exist, such as the speed of data processing and acquisition for some techniques, technical limitations, and expense for some types of equipment. They also indicated limitations related to the object interaction with the sensor, such as low contrast in X-ray images in fruit soft tissue and the limitation in infrared penetration in opaque and broad skin fruit. As a recommendation for future research, the authors indicated the standardization of confirmed efficacious procedures and made them feasible for broad applications. Deep learning algorithms enable feature extraction and the accurate detection of mechanical damage in the early development stages. However, the research on deep learning applications should be expanded to other upcoming techniques such as thermography, radiography, and magnetic resonance.

Vegetables Identification and Classification

Many research papers on the recognition of agricultural product recognition and classification are available. This section addresses recent research, processing methods, and the machine learning applied.

Rojas-Aranda et al. (2020)ROJAS-ARANDA, J. L. et al. Fruit Classification for Retail Stores Using Deep Learning. In: FIGUEROA MORA, K. M. et al. (Eds.). Pattern Recognition. Lecture Notes in Computer Science. Cham: Springer International Publishing, 2020. v. 12088, p. 3-13., presented an image classification method based on CNNs applied to three classes of fruits, inside and outside plastic bags. The input features were RGB color, RGB histogram, and RGB centroid from K-means clustering.

Anurekha and Sankaran (2020)ANUREKHA, D.; SANKARAN, R. A. Efficient classification and grading of MANGOES with GANFIS for improved performance. Multimedia Tools and Applications, v. 79, n. 5–6, p. 4169-4184, 2020. performed mango classification by employing a genetic-based ANN combined with the fuzzy inference system. The image processing consisted initially of removing noisy images from the input dataset. Subsequently, feature extraction and feature selection were performed using a genetic algorithm. The output feature trained the neural network, and the system was used for classification and grading with an accuracy of 99.18%.

Belan et al. (2016)BELAN, P. A.; ARAÚJO, S. A.; ALVES, W. A. L. An Intelligent Vision-Based System Applied to Visual Quality Inspection of Beans. In: CAMPILHO, A.; KARRAY, F. (Eds.). Image Analysis and Recognition. Lecture Notes in Computer Science. Cham: Springer International Publishing, 2016. v. 9730, p. 801-809., presented an automatic system for the classification of common beans in Brazil, utilizing skin color by applying a multilayer perceptron neural network.

Siswantoro et al. (2020)SISWANTORO, J.; ARWOKO, H.; WIDIASRI, M. Indonesian fruits classification from image using MPEG-7 descriptors and ensemble of simple classifiers. Journal of Food Process Engineering, v. 43, n. 7, 2020., employed MPEG-7 descriptors and classifiers (naive Bayesian, k-nearest neighbor, linear discriminant analysis, and decision tree) to distinguish fruits from Indonesia with 97.80% accuracy. With MPEG-7 descriptors, color and texture descriptors are obtained directly from the pixels without pre-processing or segmentation, and this system can be used both in general stores and food corporations.

Classification of Ripening Stages

The selection of fruits according to the ripening stages enables post-harvest activities to be conducted automatically, accelerating the production and packaging stages, and reducing repetitive human activities. Thus, some studies reported the application of computer vision for the classification of fruit ripening stages.

Mazen and Nashat (2019)MAZEN, F. M. A.; NASHAT, A. A. Ripeness Classification of Bananas Using an Artificial Neural Network. Arabian Journal for Science and Engineering, v. 44, n. 8, p. 6901-6910, 2019. used an automatic computer vision system to determine the ripening stages of bananas by employing an ANN-based framework and features based on color, skin spots, and Tamura statistical texture. Comparing the results with other methods (SVM, naive Bayes, KNN, decision tree, and discriminant analysis classifiers), the considered system exhibited the highest recognition rate (97.75%).

Jiang et al. (2020b)JIANG, Y. et al. identification of tomato maturity based on multinomial logistic regression with kernel clustering by integrating color moments and physicochemical indices. Journal of Food Process Engineering, v. 43, n. 10, 2020b., developed an identification method for tomato maturity by combining color and physicochemical indices. The color was obtained by a modified K-means clustering image processing program, and traditional techniques evaluated the physicochemical parameters such as firmness, soluble solid content, and sensory evaluation. A developed multinomial logistic regression with kernel clustering analyzed the data with accuracies of 84.58 and 90.42%.

CONCLUSION AND FUTURE PERSPECTIVES

  • 1. The current interest in using computer vision systems in agriculture requires obtaining and processing images faster using new algorithms for pre-processing, feature extraction, advances in machine learning, and modeling relationships, always with more robust and intelligent vision systems. Here, a tendency to reduce the requirement for processing is the use of smaller and cheaper hardware;

  • 2. According to each type of application, the sensors will also evolve to be more robust, smaller, and cheaper. In the various machines for in-field activities, post-harvest, and sorting machines, there is a tendency to combine several sensors to compose equipment. This combination makes machines easier and faster to manipulate and appropriate for several applications. Thus, the combination of data from several sensors enables machines to be more autonomous and intelligent. Machines for harvesting, sowing, and pesticides can use vision data combined with global positioning system information and weather data to perform their tasks. Fruit sorting machines can combine sensor data at different wavelengths of the electromagnetic spectrum to more accurately detect information such as chemical components, ripeness, and damage to fruits and vegetables. Similarly, nondestructive techniques may detect the surface and inside of the products, including improving 3D vision techniques that enable reconstructing fruits even with occlusion;

  • 3. Finally, please note that new developments in data science and AI have a decisive effect on computer vision and, thereby, on Agriculture 4.0. Machines are increasingly able to obtain complete information on materials in a non-invasive and non-destructive manner and facilitate the reduction in costs and labor to obtain and analyze food.

ACKNOWLEDGMENTS

The authors acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), process number 424016/2016-8, for their financial support to this study. This work has also been partially funded through projects INIA RTA2015-00078-00-00, AEI PID2019-107347RR-C31, and FEDER funds.

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Edited by

Editores do artigo: Professor Daniel Albiero - daniel.albiero@gmail.com e Professor Alek Sandro Dutra - alekdutra@ufc.br

Publication Dates

  • Publication in this collection
    20 Aug 2021
  • Date of issue
    2020

History

  • Received
    07 Jan 2020
  • Accepted
    22 Oct 2020
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