ABSTRACT
In Thai traditional medicine, Pluchea indica (L.) Less., Asteraceae, leaf has been widely used for the treatment of diabetes mellitus, tumors, hypertension, cystitis, and wounds. P. indica herbal tea is commercially available in Thailand as a health-promoting drink. The study was conducted to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the quantitative analysis of chlorogenic acid, 3,4-O-dicaffeoylquinic acid, and 3,5-O-dicaffeoylquinic acid in P. indica leaf extract and their commercial products in Thailand. The method was validated according to ICH guidelines. The proposed HPTLC method showed acceptable validation parameters. The content of chlorogenic acid, 3,4-O-dicaffeoylquinic acid, and 3,5-O-dicaffeoylquinic acid in P. indica leaves from seven different provinces in Thailand was in the range of not detectable −1.94 ± 0.02%w/w, 0.71 ± 0.01–1.89 ± 0.05%w/w, and 1.00 ± 0.01–2.18 ± 0.03%w/w, respectively, while in the commercial products, it was in the range of 0.59 ± 0.03–2.17 ± 0.05%w/w, 0.53 ± 0.04–3.77 ± 0.03%w/w, and 0.88 ± 0.05–4.72 ± 0.10%w/w, respectively. The results indicated that plantation of P. indica in coastal saline land would be beneficial as it would increase the concentration of its active compounds and improve its medicinal quality. The developed high-performance thin-layer chromatography could be used as a rapid, reliable, less demanding, and cost-effective analytical method.
Keywords:
Caffeoylquinic acid; Chlorogenic acid; Herbal tea; HPTLC; Validate
Introduction
Pluchea indica (L.) Less., belonging to Asteraceae family, is a perennial shrub species found in Australia, and many Asian countries such as India, Indonesia, Malaysia, Taiwan, and Thailand. It can reach up to 2 m in height, and it naturally grows in wet saline habitats (eFloras, 2008eFloras, 2008. Flora of China, http://www.efloras.org (accessed June 2018).
http://www.efloras.org...
). P. indica leaves have a natural sweet taste and a biting flavor and normally consumed as a side dish or herbal tea (Office of Mangrove Resources Conservation, 2009Office of Mangrove Resources Conservation, 2009. Department of Marine and Coastal Resources, Ministry of Natural Resources and Environment. Plants in the Mangrove Forest of Thailand. House of the Agricultural Co-operative Federation of Thailand Limited, Nonthaburi, pp. 7–8.). This herbal tea has a pleasant flavor, is light to dark green-brown color, and also used in traditional medicine owing to its diuretic, antipyretic, anti-inflammatory, antidiabetic, and antioxidant properties (Pramanik et al., 2006Pramanik, K.C., Bhattacharya, P., Biswas, R., Bandyopadhyay, D., Mishra, M., Chatterjee, T.K., 2006. Hypoglycemic and antihyperglycemic activity of leaf extract of Pluchea indica Less. Orient. Pharm. Exp. Med. 6, 232-236.; Buapool et al., 2013Buapool, D., Mongkol, N., Chantimal, J., Roytrakul, S., Srisook, E., Srisook, K., 2013. Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models of inflammation. J. Ethnopharmacol. 146, 495-504.; Widyawati et al., 2014Widyawati, P.S., Budianta, T.D.W., Kusuma, F.A., Wijaya, E.L., 2014. Difference of solvent polarity to phytochemical content and antioxidant activity of Pluchea indicia Less leaf extracts. Int. J. Pharmacogn. Phytochem. Res. 6, 850-855.; Vongsak et al., 2018Vongsak, B., Kongkiatpaiboon, S., Jaisamut, S., Konsap, K., 2018. Comparison of active constituents, antioxidant capacity, and α-glucosidase inhibition in Pluchea indica leaf extracts at different maturity stages. Food Biosci. 25, 68-73.). In addition, previous phytochemical investigations showed that the leaf extracts contain caffeoylquinic derivatives such as chlorogenic acid (CGA), 3,4-O-dicaffeoylquinic acid (3,4 diCQA), 3,5-O-dicaffeoylquinic acid (3,5 diCQA), quercetin, kaempferol, myricetin, monoterpenes, and sesquiterpenoids (Andarwulan et al., 2010Andarwulan, N., Batari, R., Sandrasari, D.A., Bolling, B., Wijaya, H., 2010. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem. 121, 1231-1235.; Widyawati et al., 2013Widyawati, P.S., Wijaya, C.H., Hardjosworo, P.S., Sajuthi, D., 2013. Volatile compounds of Pluchea indica Less and Ocimum basillicum Linn essential oil and potency as antioxidant. HAYATI J. Biosci. 2, 117-126.; Arsiningtyas et al., 2014Arsiningtyas, I.S., Gunawan-Puteri, M.D., Kato, E., Kawabata, J., 2014. Identification of α-glucosidase inhibitors from the leaves of Pluchea indica (L.) Less., a traditional Indonesian herb: promotion of natural product use. Nat. Prod. Commun. 28, 1350-1353.; Kongkiatpaiboon et al., 2018Kongkiatpaiboon, S., Chewchinda, S., Vongsak, B., 2018. Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand. Rev. Bras. Farmacogn. 28, 145-150.). CGA has antioxidant, antibacterial, anti-inflammatory, and hypoglycemic properties (Yu et al., 2018Yu, F., Qian, H., Zhang, J., Sun, J., Ma, Z., 2018. Simultaneous quantification of eight organic acid components in Artemisia capillaris Thunb (Yinchen) extract using high-performance liquid chromatography coupled with diode array detection and high-resolution mass spectrometry. J. Food Drug Anal. 26, 788-795.). diCQAs are potent alpha glucosidase inhibitors and prevent virus replication in tissue culture (McDougall et al., 1998McDougall, B., King, P.J., Wu, B.W., Hostomsky, Z., Reinecke, M.G., Robinson, W.E., 1998. Dicaffeoylquinic and dicaffeoyltartaric acids are selective inhibitors of human immunodeficiency virus type 1 integrase. Antimicrob. Agents Chemother. 42, 140-146.; Arsiningtyas et al., 2014Arsiningtyas, I.S., Gunawan-Puteri, M.D., Kato, E., Kawabata, J., 2014. Identification of α-glucosidase inhibitors from the leaves of Pluchea indica (L.) Less., a traditional Indonesian herb: promotion of natural product use. Nat. Prod. Commun. 28, 1350-1353.). Therefore, research on these compounds is gaining importance.
Several analytical methods have been reported for the investigation of the phytochemical compounds in P. indica extract such as GC and GC–MS, HPLC–MS/MS, and HPLC–DAD (Le et al., 2000Le, V.H., Nguyen, T.C., Nguyen, X.D., Ho, Q.T., Lecierea, P., 2000. Constituents of leaf and root essential oil of Pluchea indica (L.) Less. from Vietnam. J. Essent. Oil Bear. Plants 11, 21-28.; Shukri et al., 2011Shukri, M.A.M., Alan, C., Noorzuraini, A.R.S., 2011. Polyphenols and antioxidant activities of selected traditional vegetables. J. Trop. Agric. Food Sci. 39, 69-83.; Kongkiatpaiboon et al., 2018Kongkiatpaiboon, S., Chewchinda, S., Vongsak, B., 2018. Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand. Rev. Bras. Farmacogn. 28, 145-150.). Each method has its own limitations. For instance, GC is used for the examination of volatile compounds. Volatile compounds are vaporized at the inlet temperature and separated later in the gas phase. This separation makes GC perfect for the investigation of volatile complex compounds but this technique is not useful for non-volatile constituents (Heshka and Hager, 2015Heshka, N.E., Hager, D.B., 2015. Measurement of H2S in crude oil and crude oil headspace using multidimensional gas chromatography, deans switching and sulfur-selective detection. J. Vis. Exp. , e53416.). HPLC analysis involves the transfer of compounds from one mobile phase to another to elute at different times; however, this complex functionality is associated with miscibility issues and high back pressure (Petruczynik et al., 2008Petruczynik, A., Waksmundzka-Hajnos, M., Plech, T., Tuzimski, T., Hajnos, M.L., Jozwiak, G., Gadzikowska, M., Rompala, A., 2008. TLC of alkaloids on cyanopropyl bonded stationary phases. Part II. Connection with RP18 and silica plates. J. Chromatogr. Sci. 46, 291-297.). High-performance thin-layer chromatography (HPTLC) offers lesser area for separation of compounds and has lesser plate efficiency than HPLC and GC but it remains a valuable technique for the quality control analysis of herbal medicines as it is simple, cost-effective, and less demanding. It has been commercially utilized in fingerprint analysis to characterize and quantify numerous commercial herbal formulations and natural products at nanogram levels (Kharat et al., 2017Kharat, S., Namdeo, A., Mehta, P., 2017. Development and validation of HPTLC method for simultaneous estimation of curcumin and galangin in polyherbal capsule dosage form. J. Taibah Univ. Sci. 11, 775-781.).
Thus, the aim of the study was to develop and validate an HPTLC analytical method for the quality control of P. indica leaf extract using CGA, 3,4 diCQA, and 3,5 diCQA as quality markers. Several samples obtained from natural habitats and commercial products were analyzed.
Materials and methods
Chemicals and reagents
Standard CGA, 3,4 diCQA, and 3,5 diCQA, (purity >98%) were purchased from Chengdu Biopurify Phytochemicals Ltd., China. All other reagents used were of analytical grade.
Plant material
Mature leaves of Pluchea indica (L.) Less., Asteraceae, were collected from the following seven different provinces in Thailand: (1) Chanthaburi; (2) Chonburi; (3) Petchaburi; (4) Samut Songkhram; (5) Uttaradit; (6) Nakhon Ratchasima; (7) Songkhla; from July to August 2016. The specimens (voucher number PB16001–PB16007) were identified using the identification key provided in Flora of Thailand and deposited at Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand. The leaves were washed with tap water and dried in a hot air oven (Memmert, Germany) at 50 °C until a constant weight was achieved. The dried leaves were ground to pass through a 0.5-mm sieve and kept at −20 °C for further studies.
Commercial products including two samples of P. indica loose leaves tea, three samples of P. indica herbal tea, and three samples of P. indica capsules were purchased from local herbal drug stores in Bangkok as shown in Box 1.
Sample extraction
From our previous study (Kongkiatpaiboon et al., 2018Kongkiatpaiboon, S., Chewchinda, S., Vongsak, B., 2018. Optimization of extraction method and HPLC analysis of six caffeoylquinic acids in Pluchea indica leaves from different provenances in Thailand. Rev. Bras. Farmacogn. 28, 145-150.), ultrasound extraction with 50% ethanol yielded the highest concentration of caffeoylquinic acid derivatives in the P. indica leaf extract. Briefly, the powdered leaves were extracted with 50% ethanol (1:20, w/v) using an ultrasonic bath. The extraction was performed in triplicate. The pooled extract was filtered through Whatman no. 1 paper. The filtrate was dried under reduced pressure at 50 °C using a rotary evaporator. The crude extract was kept in a tight container protected from light at 0 °C.
For commercial products, each sample was ground to pass through a 0.5-mm sieve, then extracted with 50% ethanol as described above.
Preparation of sample solution
Each sample was prepared by accurately weighing (0.1 g) P. indica leaf extract, dissolved in methanol and adjusted to 10 ml in a volumetric flask. After complete dissolution by sonicating for 30 min, each solution was filtered through a 0.45-µm nylon membrane filter before being applied to the HPTLC plate (2 µl/band; n = 3).
Preparation of standard solution
CGA, 3,4 diCQA, and 3,5 diCQA standards were accurately weighed and dissolved in methanol in a volumetric flask for the preparation of stock solutions (1 mg/ml). Working standard solutions of CGA, 3,4 diCQA, and 3,5 diCQA were prepared to obtain a final concentration of 100 µg/ml.
Instrument and chromatographic condition
HPTLC was performed on an aluminum sheet of silica gel 60 F254 (20 cm × 10 cm, Cat. No. 1.05548.0001, Merck). Sample and standard solutions were applied to the plate as 7-mm bands with a Linomat V automatic sample spotter (Camag, Switzerland) under nitrogen flow, positioned at 10 mm from the bottom of the plate. The constant application rate was 150 nl/s. The mobile phase consisted of ethyl acetate:water:formic acid:toluene (20:2:2:1, v/v). The plate was developed to a distance of 8 cm in a Camag twin trough chamber. Densitometric scanning was performed using a TLC scanner 4 (Camag, Switzerland) using the WinCATS software. The wavelength of detection was 326 nm. The dimension of the slit was 6.0 × 0.45 mm, and the scanning speed was 10 mm/s.
Method validation
The method was validated by evaluating linearity, precision, accuracy, limit of detection (LOD), limit of quantitation (LOQ), and robustness according to the ICH guidelines (ICH, 1996/2005ICH, 1996/2005. International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. Validation of Analytical Procedures: Text and Methodology. ICH, Geneva.).
Linearity
From working standard solutions of CGA, 3,4 diCQA, and 3,5 diCQA (100 µg/ml), 1–4 µl of each solution was applied to the HPTLC plate, corresponding to concentrations of 100–400 ng/band. Calibration (linearity) curves were obtained by plotting the peak area versus the concentration of each standard.
Precision
The precision of the analytical method for each compound was determined by analyzing 200, 300, and 400 ng/band of CGA, 3,4 diCQA, and 3,5 diCQA standard solutions after applying to the HPTLC plate three times on the same day for intraday precision and on three different days for interday precision. The precision was expressed as percent relative standard deviation (% RSD).
Accuracy
The accuracy of the method for each compound was evaluated by determining recovery. The recovery of CGA, 3,4 diCQA, and 3,5 diCQA was performed on a sample spiked with three concentrations of standards (50%, 100%, and 150% of the determined content of P. indica leaves) (n = 3).
LOD and LOQ
LOD and LOQ were determined based on the standard deviation (SD) of the response and the slope (S) of each calibration curve of CGA, 3,4 diCQA, and 3,5 diCQA according to the formula: LOD = 3.3(SD/S) and LOQ = 10(SD/S).
Robustness
The robustness of the method was determined by changing some chromatographic conditions at a level of 300 ng/band of CGA, 3,4 diCQA, and 3,5 diCQA. The solvent system composition was slightly changed (±5%) as 20:2:2:1, 18.75:2:2:1, 21.25:2:2:1, v/v, of ethyl acetate:water:formic acid:toluene. The time between spotting CGA, 3,4 diCQA, and 3,5 diCQA onto the HPTLC plate to development was varied in the range of 5, 15, and 30 min. The time after development to densitometric scanning was varied in the range of 5, 15, and 30 min. The %RSD of the peak areas of the CGA, 3,4 diCQA, and 3,5 diCQA reference standards were calculated for all robustness variations.
Results and discussion
The mobile phase consisted of ethyl acetate:formic acid:water:toluene (20:2:2:1, v/v) gave the best peak resolution of CGA, 3,4 diCQA, and 3,5 diCQA. The specificity of the bands of CGA (Rf = 0.34), 3,4 diCQA (Rf = 0.63), and 3,5 diCQA (Rf = 0.79) in P. indica leaf extract was confirmed by overlaying the absorption spectra of the samples with those of CGA, 3,4 diCQA, and 3,5 diCQA reference standards (Fig. 1). The specificity of the analyzed peaks was checked at three different peak levels i.e., start, apex, and end positions of the peaks corresponding to CGA, 3,4 diCQA, and 3,5 diCQA.
Overlay UV spectra scanning from 200 to 700 nm by HPTLC method. (A) CGA reference standard and sample; (B) 3,4 diCQA reference standard and sample; (C) 3,5 diCQA reference standard and sample.
The proposed HPTLC method showed acceptable validation parameters (Table 1). The calibration curve of CGA, 3,4 diCQA, and 3,5 diCQA was linear over the range of 100–400 ng/band. The correlation coefficient value was ≥0.995, confirming the linearity of the method. The % RSD value of intra- and interday precision was <5%. The LOD of CGA, 3,4 diCQA, and 3,5 diCQA was found to be 9.92, 17.58, and 6.68 ng/band, while the LOQ of CGA, 3,4 diCQA, and 3,5 diCQA was found to be 30.05, 53.27, and 20.24 ng/band, respectively. The average recovery of CGA, 3,4 diCQA, and 3,5 diCQA was 99.04 ± 5.21, 97.51 ± 4.14, and 99.64 ± 9.65%, respectively. The validation parameters indicated both good precision and accuracy of the analytical method. For robustness studies, %RSD was found to be less than 5% for all variations.
The content of CGA, 3,4 diCQA, and 3,5 diCQA in P. indica leaves from seven different provinces was in the range of not detected −1.94 ± 0.02%w/w, 0.71 ± 0.01–1.89 ± 0.05%w/w, and 1.00 ± 0.01–2.18 ± 0.03%w/w, respectively (Table 2). The HPTLC chromatograms of reference standards, P. indica leaf extract, and its commercial products are shown in Fig. 2.
Content of CGA, 3,4 diCQA, and 3,5 diCQA in Pluchea indica leaf extract and its commercial products determined by the validated HPTLC densitometric method.
HPTLC chromatograms. (A) CGA, 3,4diCQA and 3,5 diCQA standard; (B) Pluchea indica leaves from Uttaradit province; (C) Commercial product (Brand B).
Pluchea indica leaves from the Nakhon Ratchasima province (Northeastern region) contained the lowest amount of three major compounds. It might be because of the poor quality of soil in the northeastern mountains, which are generally less fertile, and because temperatures are considerably warmer here than in the other parts of the country (Choenkwan et al., 2014Choenkwan, S., Fox, J.M., Rambo, A.T., 2014. Agriculture in the mountains of northeastern Thailand: current situation and prospects for development. Mt. Res. Dev. 34, 95-106.). P. indica commonly grows in saline habitats such as brackish marshes and mangroves. Numerous studies reported that NaCl stress can induce the accumulation of phenolic compounds in plant tissues. Yan et al. (2016)Yan, K., Cui, M., Zhao, S., Chen, X., Tang, X., 2016. Salinity stress is beneficial to the accumulation of chlorogenic acids in honeysuckle (Lonicera japonica Thunb.) Front. Plant Sci. 7, 1563. reported that the concentration of CGA in honeysuckle leaves was higher in saline than in non-saline fields (Yan et al., 2016Yan, K., Cui, M., Zhao, S., Chen, X., Tang, X., 2016. Salinity stress is beneficial to the accumulation of chlorogenic acids in honeysuckle (Lonicera japonica Thunb.) Front. Plant Sci. 7, 1563.). Borgognone et al. (2014)Borgognone, D., Cardarelli, M., Rea, E., Lucini, L., Colla, G., 2014. Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system. J. Sci. Food Agric. 94, 1231-1237. also reported that salt stress (NaCl and KCl) could increase total phenolic, total flavonoids, and antioxidant activity in leaves of artichoke and cardoon (Borgognone et al., 2014Borgognone, D., Cardarelli, M., Rea, E., Lucini, L., Colla, G., 2014. Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system. J. Sci. Food Agric. 94, 1231-1237.). In agreement with our result, the content of CGA, 3,4 diCQA, and 3,5 diCQA in P. indica leaf extract from coastal provinces (Songkhla, Chanthaburi, and Chonburi) was higher than that in the extract from other regions. Therefore, plantation of P. indica in coastal saline land would be beneficial as it would increase the concentration of its active compounds and improve its medicinal quality.
For P. indica commercial products, the content of CGA, 3,4 diCQA, and 3,5 diCQA was in the range of 0.59 ± 0.03–2.17 ± 0.05%w/w, 0.53 ± 0.04–3.77 ± 0.03%w/w, and 0.88 ± 0.05–4.72 ± 0.10%w/w, respectively (Table 1). Drying reduces moisture content, thereby inhibiting enzymatic degradation and limiting bacterial growth in herbal tea products. This essential step in tea processing influences the appearance and antioxidant activity of tea, which affect the its commercial value (Chong and Lim, 2012Chong, K.L., Lim, Y.Y., 2012. Effects of drying on the antioxidant properties of herbal tea from selected Vitex species. J. Food Qual. 35, 51-59.). Drying significantly affects the content of caffeoylquinic acid derivatives including CGA, 4,5 diCQA, 3,5 diCQA, 3,4 diCQA, and 3,4,5 triCQA in sweet potato leaves. Hot air drying at 70 °C and 100 °C significantly decreases the amount of caffeoylquinic acid derivatives and their antioxidant activities. Among the different drying methods, freeze drying and cool air drying at 30 °C were found to retain higher amounts of caffeoylquinic acid derivatives (Jeng et al., 2015Jeng, T.L., Lai, C.C., Liao, T.C., Lin, S.Y., Sung, J.M., 2015. Effects of drying on caffeoylquinic acid derivative content and antioxidant capacity of sweet potato leaves. J. Food Drug Anal. 23, 701-708.). Similarly, oven drying under higher temperatures (70 °C and 100 °C) significantly decreases total phenolic content and antioxidant activity compared with heat treatment at 40 °C and 50 °C for Vitex negundo L. tea (Rabeta and Vithyia, 2013Rabeta, M.S., Vithyia, M., 2013. Effect of different drying methods on the antioxidant properties of Vitex negundo Linn. tea. Int. Food Res. J. 20, 3171-3176.). Katsube et al. (2009)Katsube, T., Tsurunaga, Y., Sugiyama, M., Furuno, T., Yamasaki, Y., 2009. Effect of air-drying temperature on antioxidant capacity and stability of polyphenolic compounds in mulberry (Morus alba L.) leaves. Food Chem. 113, 964-969. also reported that an air drying at 70 °C significantly decreased DPPH radical scavenging activity and the content of polyphenolic compounds of mulberry leaf (Katsube et al., 2009Katsube, T., Tsurunaga, Y., Sugiyama, M., Furuno, T., Yamasaki, Y., 2009. Effect of air-drying temperature on antioxidant capacity and stability of polyphenolic compounds in mulberry (Morus alba L.) leaves. Food Chem. 113, 964-969.). The decrease in antioxidant activity during heat treatment could be due to the initial enzymatic degradation and thermal degradation of antioxidant compounds (Cho et al., 2017Cho, C.L., Lee, Y.Z., Tseng, C.N., Cho, J., Cheng, Y.B., Wang, K.W., Chen, H.J., Chiou, S.J., Chou, C.H., Hong, Y.R., 2017. Hexane fraction of Pluchea indica root extract inhibits proliferation and induces autophagy in human glioblastoma cells. Biomed. Rep. 7, 416-422.). Moreover, caffeoylquinic acids are degraded by high temperature and light irradiation (Xue et al., 2016Xue, M., Shi, H., Zhang, J., Liu, Q.Q., Guan, J., Zhang, J.Y., Ma, Q., 2016. Stability and degradation of caffeoylquinic acids under different storage conditions studied by high-performance liquid chromatography with photo diode array detection and high-performance liquid chromatography with electrospray ionization collision-induced dissociation tandem mass spectrometry. Molecules 21, http://dx.doi.org/10.3390/molecules21070948.
http://dx.doi.org/10.3390/molecules21070...
). In accordance with our results, the P. indica loose leaf tea and herbal tea formulations showed lower content of the three major compounds than the capsule formulation. It might due to exposure to high temperatures (pan firing/steaming and oven drying) during the manufacturing process of loose leaf tea. Thus, exposure to high drying temperatures should be avoided during the manufacturing process of P. indica products and the products must be stored in light-proof containers.
HPTLC was developed for quantification of CGA, 3,4 diCQA, and 3,5 diCQA in P. indica leaf extract and its commercial products. The developed method showed acceptable validation parameters according to ICH guidelines. HPTLC is a simple, rapid, and cost-effective analytical technique. It is one of the most widely applied method for the analysis of herbal extracts, which have a complex mixture of many chemical constituents. It is used for screening for adulterations, quantitative analysis of major compounds, and testing of stability. In conclusion, this developed method can be used as an alternative analytical method in the routine quality control of commercial products of P. indica leaves.
Acknowledgements
This study was financially supported by the Research Grant of Burapha University through National Research Council of Thailand (Grant No. 23/2559) and Faculty of Pharmaceutical Sciences, Burapha University.
References
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» http://dx.doi.org/10.3390/molecules21070948
Publication Dates
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Publication in this collection
27 May 2019 -
Date of issue
Mar-Apr 2019
History
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Received
23 Aug 2018 -
Accepted
12 Dec 2018 -
Published
29 Jan 2019