Microscopia electrônica de inclusões citoplas máticas e alterações celulares associadas ao vírus do mosaico da cenoura

Camargo, I. J. B.; Kitajima, E. W.; Costa, A. S.

doi:10.1590/S0006-87051971000100004

Resumos

Exames electrono-microscópicos de secções ultrafinas de tecidos foliares de cenoura (Daucus carota L.) e coentro (Coriandrum sativumL.) infetados pelo vírus do mosaico da cenoura (VMC) revelaram a ocorrência de inclusões em forma de bandas densas no citoplasma. Exames mais detalhados dessas bandas revelaram serem elas constituídas pela justaposição de lamelas delgadas (10-15m¼ de espessura), as quais parecem eventualmente desprender-se e formar outros tipos de inclusão, como as de perfil circular e em forma de catavento, comumente associadas à infecção provocada por vírus do grupo Y da batata. Ocasionalmente, partículas do VMC foram observadas nas proximidades dessas inclusões. As dimensões das bandas densas atingiram até 0,l-0,2¼ x 3-4¼, visíveis mesmo em exames ao microscópio convencional. Além da ocorrência dessas inclusões, pôde também ser observada a presença de grande número de vesículas no citoplasma, aparentemente associadas a uma atividade do complexo de Golgi.

A virus, inducing mosaic and malformation of carrot leaves (Daucus carota L.), was found in Piedade, SP. It was termed carrot mosaic virus (CMV), and is both mechanically and aphid transmitted. Morphologically it belongs to the potato virus Y group (15m¼ x 740m¼). Electron microscopic examination of thin sections of leaf tissues from carrot or coriander (Coriandrum sativum L.) infected with CMV demonstrated the presence of cytoplasmic inclusions, usually in the form of dense bands. They could reach sizes up to 0.2¼ x 4¼ and were detectable even under light microscopic examination. These bands, at higher magnification, showed to be built up of thin lamella (10-15m¼ thick), closely apposed. Occasionally individual or group of lamella appeared to loose from the bands, producing other type of profile, such as pin wheels and rings. CMV particles were sometimes observed in the vicinity of these inclusions, often laying parallel to their surface. These inclusions are identical to those observed in tissues infected with other components of the potato Y virus group, and their detection seems to corroborate the previous inclusion of CMV in this group, basead on particle morphology. Besides these cytoplasmic inclusions, some cells contained a large number of vesicles in the cytoplasm, apparently associated with Golgi complex activity.

Microscopia electrônica de inclusões citoplas máticas e alterações celulares associadas ao vírus do mosaico da cenoura¹ 1 Trabalho apresentado na XX Reunião Anual da Sociedade Brasileira para o Progresso da Ciência, realizada em São Paulo, SP, em julho de 1968. Esta pesquisa recebeu auxílio da PAPESP (C. Aeron. 69/879) CNPq (TC-12.275) e do convênio CAPES/BID/BNDE/.

Electron microscopy of cytoplasmic inclusion and cell modification associated with carrot mosaic virus

I. J. B. Camargo^I,² 2 Bolsista do CNPq. ; E. W. Kitajima^II,² 2 Bolsista do CNPq. ; A. S. Costa^II,² 2 Bolsista do CNPq.

^IBiologista

^IIEngenheiros-agrônomos, Seção de Virologia Fitotécnica, Instituto Agronômico

SINOPSE

Exames electrono-microscópicos de secções ultrafinas de tecidos foliares de cenoura (Daucus carota L.) e coentro (Coriandrum sativumL.) infetados pelo vírus do mosaico da cenoura (VMC) revelaram a ocorrência de inclusões em forma de bandas densas no citoplasma. Exames mais detalhados dessas bandas revelaram serem elas constituídas pela justaposição de lamelas delgadas (10-15mμ de espessura), as quais parecem eventualmente desprender-se e formar outros tipos de inclusão, como as de perfil circular e em forma de catavento, comumente associadas à infecção provocada por vírus do grupo Y da batata. Ocasionalmente, partículas do VMC foram observadas nas proximidades dessas inclusões.

As dimensões das bandas densas atingiram até 0,l-0,2μ x 3-4μ, visíveis mesmo em exames ao microscópio convencional. Além da ocorrência dessas inclusões, pôde também ser observada a presença de grande número de vesículas no citoplasma, aparentemente associadas a uma atividade do complexo de Golgi.

SUMMARY

A virus, inducing mosaic and malformation of carrot leaves (Daucus carota L.), was found in Piedade, SP. It was termed carrot mosaic virus (CMV), and is both mechanically and aphid transmitted. Morphologically it belongs to the potato virus Y group (15mμ x 740mμ).

Electron microscopic examination of thin sections of leaf tissues from carrot or coriander (Coriandrum sativum L.) infected with CMV demonstrated the presence of cytoplasmic inclusions, usually in the form of dense bands. They could reach sizes up to 0.2μ x 4μ and were detectable even under light microscopic examination. These bands, at higher magnification, showed to be built up of thin lamella (10-15mμ thick), closely apposed. Occasionally individual or group of lamella appeared to loose from the bands, producing other type of profile, such as pin wheels and rings. CMV particles were sometimes observed in the vicinity of these inclusions, often laying parallel to their surface.

These inclusions are identical to those observed in tissues infected with other components of the potato Y virus group, and their detection seems to corroborate the previous inclusion of CMV in this group, basead on particle morphology.

Besides these cytoplasmic inclusions, some cells contained a large number of vesicles in the cytoplasm, apparently associated with Golgi complex activity.

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LITERATURA CITADA

Recebido para publicação em 10 de agôsto de 1970.

1. BRANDES, J. & WETTER, C. Classification of elongated plant viruses on the basis of particle morphology. Virology 8:95-115, 1959.
2. CAMARGO, I. J. B.; KITAJIMA, E. W. & COSTA, A. S. Estudo ao microscópio electrônico de tecidos de plantas infetadas pelo vírus do mosaico comum e amarelo de feijoeiro. Bragantia 27:409-420, 1968.
3. COSTA, A. S.; KITAJIMA, E. W. & CAMARGO, I. J. B. Ocorręncia do mosaico em cenoura no Estado de Săo Paulo. Trabalho apresentado na IX Reuniăo Anual da Sociedade de Olericultura do Brasil, Goiânia, GO., 1969.
4. EDWARDSON, J. R. Cylindrical inclusions in the cytoplasm of leaf cells infected with tobacco etch virus. Science 153:883-884, 1966.
5. ________; PURCIFULL, D. E. & CHRISTIE, R. G. Structure of cytoplasmic inclusions in plants infected with rodshaped viruses. Virology 34:250-263, 1968.
6. HAYASHI, T.; MATSUI, C. & YAMAGUCHI, A. Electron microscopy of intracelular turnip mosaic virus. Phytopathology 55: 478, 1965.
7. KAZUMOBU, A. & DALES, S. Cytopathology of Mengovirus infection. II. Proliferation of membranous cisternae. Virology 32:201-215, 1967.
8. KITAJIMA, E. W.; CAMARGO, I. J. B. & COSTA, A. S. Intra-nuclear crystals and cytoplasmic membranous inclusions associated with infection by two Brazilian strains of potato virus Y. Journal of Electron Microscopy 17:144-153, 1968.
9. ________; ________ & ________. Morfologia do virus do mosaico da cenoura. Bragantia 27:13-15, 1968.
10. ________; LAURITIS, J. & SWIFT, H. Fine structure of zinnia leaf tissues infected with dahlia mosaic virus. Virology 39: 240-249, 1969.
11. LUFT, L. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9:409-414, 1961.
12. MATSUI, C. & YAMAGUCHI, A. Electron microscopy of host cells infected with tobacco etch virus. I. Fine structure of leaf cells at later stages on infection. Virology 22:40-47, 1964.
13. MILLONING, G. Studio sui fattori che determinano la preservazione della ultraestructtura. In: Buffa, P. ed. From molecule to cell. Symposium on Electron Miscroscopy. Roma, 1964. p. 363-366.
14. PURCIFULL, D. E. & EDWARDSON, J. R. Watermelon mosaic virus: Tubular inclusions in pumpkim leaves and aggregates in leaf extracts. Virology 32:393-401, 1967.
15. REYNOLDS, E. S. The use of leaf citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:204-211, 1963.
16. RICHARDSON, N. K.; JARRET, L. & FINKE, H. Embedding in epoxy resins for ultra-thin section in electron microscopy. Stain Technol. 35:313-325, 1960.
17. WATSON, M. L. Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4:475-478, 1958.
18. WEITRAUB, M. & RAGETLI, H. W. J. Fine structure of inclusions and organelles in Vicia fabainfected with bean yellow mosaic virus. Virology 28:390-402, 1966.
19. YAMAGUCHI, A.; KIKUMOTO, T. & MATSUI, C. Electron microscopy of elongated particles associated with tulip mosaic. Virology 20:143, 1963.

1

Trabalho apresentado na XX Reunião Anual da Sociedade Brasileira para o Progresso da Ciência, realizada em São Paulo, SP, em julho de 1968. Esta pesquisa recebeu auxílio da PAPESP (C. Aeron. 69/879) CNPq (TC-12.275) e do convênio CAPES/BID/BNDE/.

2

Bolsista do CNPq.

Datas de Publicação

Publicação nesta coleção
18 Mar 2009
Data do Fascículo
1971

Histórico

Recebido
10 Ago 1970

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BRANDES, J. & WETTER, C. Classification of elongated plant viruses on the basis of particle morphology. Virology 8:95-115, 1959.

[2] 2. CAMARGO, I. J. B.; KITAJIMA, E. W. & COSTA, A. S. Estudo ao microscópio electrônico de tecidos de plantas infetadas pelo vírus do mosaico comum e amarelo de feijoeiro. Bragantia 27:409-420, 1968.

[3] 3. COSTA, A. S.; KITAJIMA, E. W. & CAMARGO, I. J. B. Ocorręncia do mosaico em cenoura no Estado de Săo Paulo. Trabalho apresentado na IX Reuniăo Anual da Sociedade de Olericultura do Brasil, Goiânia, GO., 1969.

[4] 4. EDWARDSON, J. R. Cylindrical inclusions in the cytoplasm of leaf cells infected with tobacco etch virus. Science 153:883-884, 1966.

[5] 5. ________; PURCIFULL, D. E. & CHRISTIE, R. G. Structure of cytoplasmic inclusions in plants infected with rodshaped viruses. Virology 34:250-263, 1968.

[6] 6. HAYASHI, T.; MATSUI, C. & YAMAGUCHI, A. Electron microscopy of intracelular turnip mosaic virus. Phytopathology 55: 478, 1965.

[7] 7. KAZUMOBU, A. & DALES, S. Cytopathology of Mengovirus infection. II. Proliferation of membranous cisternae. Virology 32:201-215, 1967.

[8] 8. KITAJIMA, E. W.; CAMARGO, I. J. B. & COSTA, A. S. Intra-nuclear crystals and cytoplasmic membranous inclusions associated with infection by two Brazilian strains of potato virus Y. Journal of Electron Microscopy 17:144-153, 1968.

[9] 9. ________; ________ & ________. Morfologia do virus do mosaico da cenoura. Bragantia 27:13-15, 1968.

[10] 10. ________; LAURITIS, J. & SWIFT, H. Fine structure of zinnia leaf tissues infected with dahlia mosaic virus. Virology 39: 240-249, 1969.

[11] 11. LUFT, L. Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9:409-414, 1961.

[12] 12. MATSUI, C. & YAMAGUCHI, A. Electron microscopy of host cells infected with tobacco etch virus. I. Fine structure of leaf cells at later stages on infection. Virology 22:40-47, 1964.

[13] 13. MILLONING, G. Studio sui fattori che determinano la preservazione della ultraestructtura. In: Buffa, P. ed. From molecule to cell. Symposium on Electron Miscroscopy. Roma, 1964. p. 363-366.

[14] 14. PURCIFULL, D. E. & EDWARDSON, J. R. Watermelon mosaic virus: Tubular inclusions in pumpkim leaves and aggregates in leaf extracts. Virology 32:393-401, 1967.

[15] 15. REYNOLDS, E. S. The use of leaf citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17:204-211, 1963.

[16] 16. RICHARDSON, N. K.; JARRET, L. & FINKE, H. Embedding in epoxy resins for ultra-thin section in electron microscopy. Stain Technol. 35:313-325, 1960.

[17] 17. WATSON, M. L. Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol. 4:475-478, 1958.

[18] 18. WEITRAUB, M. & RAGETLI, H. W. J. Fine structure of inclusions and organelles in Vicia fabainfected with bean yellow mosaic virus. Virology 28:390-402, 1966.

[19] 19. YAMAGUCHI, A.; KIKUMOTO, T. & MATSUI, C. Electron microscopy of elongated particles associated with tulip mosaic. Virology 20:143, 1963.