INFECTION BY Rickettsia felis IN OPOSSUMS (Didelphis sp.) FROM YUCATAN, MEXICO

Abstract

Rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the Americas. Its transmission cycle involves fleas as biological vectors (mainly Ctenocephalides felis) and multiple domestic and synanthropic mammal hosts. Nonetheless, the role of mammals in the cycle of R. felis is not well understood and many efforts are ongoing in different countries of America to clarify it. The present study describes for the first time in Mexico the infection of two species of opossum (Didelphis virginiana and D. marsupialis) by R. felis. A diagnosis was carried out from blood samples by molecular methods through the gltA and 17 kDa genes and sequence determination. Eighty-seven opossum samples were analyzed and 28 were found to be infected (32.1%) from five out of the six studied localities of Yucatan. These findings enable recognition of the potential epidemiological implications for public health of the presence of infected synanthropic Didelphis in households.

Rickettsia felis; gltA; 17 kDa; Transmission cycle; Didelphis marsupialis; Didelphis virginiana; Yucatan

INTRODUCTION

Rickettsia felis is an emergent pathogen that infects humans and is the causative agent of the probably underestimated flea-borne spotted fever^1,2. The general transmission cycle of R. felis has been described as a vector-borne disease and fleas acquire the bacteria mainly by vertical infection and apparently less frequently by horizontal infection through other fleas or infected vertebrate hosts³.

The agent Rickettsia felis has been reported in different countries around the world to infect its biological vector, the cat flea Ctenocephalides felis, four domestic or synanthropic hosts (cats, dogs, rats and opossums), and humans^2,4,5,6,7. In Mexico, Yucatan is the only region where human cases have been reported^7,8, mainly from rural and suburban localities where people are in closer contact with sylvan and synanthropic mammals and their ectoparasites^7,9. The most common wild synanthropes in Yucatan are opossums (Didelphis spp.) and rodents, which can potentially harbor flea-borne zoonotic pathogens and carry them to domestic and peridomestic areas since they are frequently infested with fleas^10,11. Records of C. felis collected in Didelphis virginiana, cats, and dogs are common^1,3,12,13. Opossums have been found infected with R. felis in some countries of the Americas but not in Mexico^4,5,6.

The aim of the present study is to report, for the first time in Mexico, the finding of this rickettsial infection in opossums captured in six different localities in Yucatan, which were assessed by systematic and occasional field trips monitoring of the presence of relevant zoonotic pathogens to public health in the peninsula.

MATERIALS AND METHODS

Study area and opossum trapping. Opossums were captured from six localities: Kikil, Cholul (July to November 2005), Dzidzilche, Dzitya, Molas (July to December 2011), and Buctzotz (July to December 2012) in the State of Yucatan (Fig. 1, see Table 1 for geographical coordinates). The climate is predominantly tropical sub-humid, with a rainy season from June to November, and a dry season from December to May. The vegetation of the region is predominantly tropical deciduous forest but human activities have modified extensive areas for urban, agriculture, farming, and livestock practices⁸.

Fig. 1
- Geographic distributions of localities where opossum captures were carried out in the Yucatan.

Table 1
Locality, species identity, and ecotope of Didelphis opossums captured/infected (+) and percentage of individuals (%) infected by Rickettsia felis diagnosed by PCR of blood samples tested with the gltA and 17 kDa genes

Locality	Geographic Coordinates	Opossum species	Ecotope	Captured/+ (%)
Kikil	21°11'32.47"N, 88°10'6.17"W	D. marsupialis D. virginiana	Sylvatic Peridomestic	1/1 (100) 2/2 (100)
Dzitya	21°3'1.96"N, 89°40'39.30"W	D. virginiana	Peridomestic	8/0
Cholul	21°2'24.98"N, 89°33'19.31"W	D. virginiana	Peridomestic	26/11 (42.3)
Molas	20°49'0.15"N, 89°37'52.78"W	D. virginiana	Peridomestic	10/3 (30)
Buctzotz	21°12'8.17"N, 88°47'36.23"W	D. virginiana	Peridomestic	32/9 (28.1)
Dzidzilche	21°9'8.75"N, 89°41'24.39"W	D. virginiana	Peridomestic Sylvatic	7/2 (28.5) 1/0
Total				87/28 (32.1)

The capture of opossums was carried out using Tomahawk Live Traps(r) with tropical fruit as bait; in the peridomiciliar area, one trap was placed in the backyard of households. The trapping period in the peridomicile varied from one to three consecutive nights. Trapping in the sylvatic areas was performed only in Kikil and Dzidzilche because in these two localities a wider study on opossum ecology was performed (nonetheless, all captured animals were included in this report). Methods consisted of a grid of 4 x 4 traps (each separated by 100 m), which was established in wild habitats located 1 to 5 km away from the communities. In the sylvatic areas, the trapping period consisted of three consecutive nights.

Blood sampling. Blood was collected from the lateral vein of the opossum's tail, preserved in 3.8% sodium citrate (anticoagulant) and maintained at 4 °C for transportation to the laboratory where it was preserved at -20 °C until analysis. All opossums were carefully manipulated and subsequently released in accordance with the protocols of Sikes & Gannon¹⁴ and the guidelines established in NOM-062-ZOO-1999¹⁵.

Rickettsia diagnosis by PCR. DNA extraction was performed using a QIAamp DNA kit (QIAGEN(r) Valencia, CA) in accordance with the manufacturer's instructions. A single-step polymerase chain reaction (PCR) was used to detect Rickettsia DNA.

The single-step PCR amplification was performed using genus-specific primers for the rickettsial 17 kDa protein gene (5'-GCTCTTGCAACTTCTATGTT-3' and 5'-CATTGTTCGTCAGG TTGGCG-3') generating an amplification product of 480 bp and the citrate synthase gene (gltA) (5'-GGGGGCCTGCTCACGGCGG-3' and 5'-ATTGCAAAAAGTACAGTGAACA-3') generating an amplification product of 384 bp as indicated in the guidelines for molecular detection (PCR and sequencing) of tick-borne rickettsiosis from the Red Iberoamericana para la Investigación y Control de Enfermedades Rickettsiales (RIICER)¹⁶. In all PCR assays, we used sterile water in one reaction as a negative control and a Rickettsia conorii DNA sample (Rickettsia species with no presence in America) as a positive control. All PCR reactions were carried in a Gene Amp PCR System 2400 Thermal Perkin Elmer^(r) thermal cycler. Negative samples to the 17 kDa gene single-step PCR were tested with a 17 kDa gene nested PCR using the forward primer 5'-CATTACTTGGTTCTCAATTCGG-3' and reverse primer 5'-GTTTTATTAGTG GTTACGTAACC-3' (232 bp), using the same amplification parameters of the single-step 17 kDa gene PCR.

The gltA and the 17 kDa gene PCR products were cloned into the TOPO TA pCR 2*1-TOPO vector (Invitrogen(r), Carlsbad, CA, USA). Two clones from the same cloning reaction were sequenced three times each using a Perkin Elmer(r) ABI Prism 377 automated sequencer. Primer sequences were removed before comparison to sequences from the GenBank database using the BLAST 2.0 software available at the National Center for Biotechnology Information (NCBI) site.

RESULTS

A total of 87 opossums were captured in the different localities. From those, 85 D. virginiana blood samples were taken from rural and suburban peridomiciles and tested: 10 from Molas, 7 from Dzidzilche, 32 from Buctzotz, 8 from Dzitya, 2 from Kikil, and 26 from Cholul; only two opossums were captured in sylvatic quadrants, one from Kikil (identified as D. marsupialis) and one D. virginiana from Dzidzilche.

A blood sample from an opossum was considered infected by Rickettsia when both PCR reactions (17 kDa and gltA) yielded positive results. The PCR procedure showed Rickettsia DNA identification in at least one opossum from each locality except for those from Dzitya. In all the positive samples, the Rickettsia DNA sequences obtained (GeneBank accession number: KR709306 and KR709307, respectively) showed 99% similarity with the gltA and the 17 kDa sequence of the R. felis strain Marseille-URRWFXCal2 (Table 1).

DISCUSSION

The opossums D. virginiana have been implicated in the zoonotic transmission of R. typhi and R. felis in the United States^4,17, and in Yucatan previous studies have reported their role as natural hosts in the peridomestic transmission cycle of other zoonotic pathogens^10,18,19,20. As far as we know, there has been no previous report of the presence of Rickettsial infection in D. marsupialis from Mexico. Yucatan State represents a sympatric region in the geographic distribution of D. marsupialis and D. virginiana in which both species clearly show different ecological requirements, with D. virginiana being more closely associated with human peridomiciles than D. marsupialis, both in rural and suburban households¹⁰.

Currently, there is no clear consensus about the role of mammals in R. felis transmission dynamics, since infection by Rickettsia sp. in these natural hosts is characterized by a very short rickettsemia^1,21. As a consequence, the probability of capturing hosts with rickettsial infection is very low in nature, which makes it difficult to understand their role in the transmission cycle. Some studies have suggested that opossums may act as an important link in the horizontal transmission as a source of R. felis to uninfected vectors or as a source of vertically infected vectors that can transmit the pathogen to other mammalian hosts and humans²², in case domestic or companion animals and synanthropic opossums could maintain the infection dynamics of R. felis in Yucatan. Both are frequently infested by C. felis, the main vector and true reservoir of R. felis^1,3,12,13, due to the frequent presence of peridomiciliar opossums, mainly D. virginiana, in the backyards of human households.

In the case of D. marsupialis, the transmission scenario in Northern Yucatan would seem to be more restricted to a sylvatic habitat. However, its role as a source of Rickettsia for fleas in human households in the Southern area of Yucatan State could be enhanced because in that region their populations are more abundant and predominant than those of D. virginiana¹¹.

The main implication of opossums infected by R. felis lies in the very close association of these marsupials with human dwellings¹¹, allowing them to potentially contribute to the transmission cycle of this vector-borne zoonotic pathogen, as suggested in a previous study¹³.

Despite the fact that the results of this study cannot be used to characterize the population of opossums in the entire Yucatan peninsula, the detection of two species of opossum infected by R. felis demonstrates the need to conduct epidemiological studies focused on this marsupial as a sentinel of the presence of Rickettsia in the peridomiciles of human dwellings.

Additionally, the first finding of infected D. marsupialis is very interesting, because this species is more restricted to sylvatic areas than peridomiciles in Yucatan and could represent an important link between these ecotopes. These findings reveal, for the first time in Mexico, the potential role of opossums in the ecology of Rickettsia transmission and their utility as sentinels of this pathogen in human dwellings.

ACKNOWLEDGMENTS

This work was supported by a research grant from CONACYT (CB-2008-1-99890) to JZC, a research grant from SEP (CIRB-PRIORI.04-004) to HARP, and a research grant from PROMEP-SEP (CIRB-2011-0003) to ERN.

REFERENCES

• ¹
  Horta MC, Sabatini GS, Moraes-Filho J, Ogrzewalska M, Canal RB, Pacheco RC, et al. Experimental infection of the opossum Didelphis aurita by Rickettsia felis, Rickettsia bellii, and Rickettsia parkeri and evaluation of the transmission of the infection to ticks Amblyomma cajennense and Amblyomma dubitatum. Vector-Borne Zoonotic Dis. 2010;10:959-67.
• ²
  Oteo JA, Portillo A, Santibañez S, Blanco JR, Pérez-Martínez, L, Ibarra V. Cluster of cases of human Rickettsia felis infection from Southern Europe (Spain) diagnosed by PCR. J Clin Microbiol. 2006;44:2669-71.
• ³
  Giudice E, Di Pietro S, Alaimo A, Blanda V, Lelli R, Francaviglia F, et al. A molecular survey of Rickettsia felis in fleas from cats and dogs in Sicily (Southern Italy). PLOS ONE. 2014;9:e106820.
• ⁴
  Labruna MB, Mattar VS, Nava S, Bermúdez S, Venzal JM, Dolz G, et al. Rickettsioses in Latin America, Caribbean, Spain and Portugal. Rev MVZ Córdoba. 2011;16:2435-57.
• ⁵
  Labruna MB, Ogrzewalska M, Moraes-Filho J, Lepe P, Gallegos JL, López J. Rickettsia felis in Chile. Emerg Infect Dis. 2007;13:1794-5.
• ⁶
  Schriefer ME, Sacci JB Jr, Taylor JP, Higgins JA, Azad AF. Murine typhus: updated roles of multiple urban components and a second typhus-like Rickettsia. J Med Entomol. 1994;31:681-5.
• ⁷
  Zavala-Velázquez JE, Ruiz-Sosa JA, Sánchez-Elias RA, Becerra-Carmona G, Walker DH. Rickettsia felis rickettsiosis in Yucatan. Lancet. 2000;356:1079-80.
• ⁸
  Durán Garcia R, Méndez González M, editors. Biodiversidad y desarrollo humano en Yucatán. Mérida: CICY, PPD-FMAM, Conabio, Seduma; 2010.
• ⁹
  Zavala-Castro J, Zavala-Velázquez J, Walker D, Pérez-Osorio J, Peniche-Lara G. Severe human infection with Rickettsia felis associated with hepatitis in Yucatan, Mexico. Int J Med Microbiol. 2009;299:529-33.
• ¹⁰
  Reyes-Novelo E, Ruiz-Piña H, Escobedo-Ortegón F, Rodríguez-Vivas I, Bolio-Gonzalez M, Polanco-Rodríguez A, et al. Situación actual y perspectivas para el estudio de las enfermedades zoonóticas emergentes, reemergentes y olvidadas en la Península de Yucatán, México. Trop Subtrop Agroecosyst. 2011;14:35-54.
• ¹¹
  Ruiz-Piña HA, Reyes-Novelo E, Escobedo-Ortegón F, Barrera-Perez M. Mamíferos sinantrópicos y la transmisión de enfermedades zoonóticas en el área rural de Yucatán. In: Pacheco Castro J, Lugo Pérez JA, Tzuc Canché L, Ruiz Piña H, editores. Estudios multidisciplinarios de las enfermedades zoonóticas y ETVs en Yucatán. Mérida: Universidad Autónoma de Yucatán; 2013. p. 184-94.
• ¹²
  Eckerlin RP. Fleas (Siphonaptera) of the Yucatan Peninsula (Campeche, Quintana Roo, and Yucatan), Mexico. Caribb J Sci. 2005;41:152-7.
• ¹³
  Zavala-Velázquez JE, Zavala-Castro JE, Vado-Solis I, Ruiz-Sosa JA, Moron CG, Bouyer DH, et al. Identification of Ctenocephalides felis fleas as a host of Rickettsia felis, the agent of a spotted fever rickettsiosis in Yucatan, Mexico. Vector Borne Zoonotic Dis. 2002;2:69-75.
• ¹⁴
  Sikes RS, Gannon WL. Guidelines of the American Society of Mammalogists for the use of wild mammals in research. J Mammal. 2011;92:235-53.
• ¹⁵
  México. Secretaria de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación. Especificaciones técnicas para el cuidado y uso de los animales de laboratorio. Ciudad de Me´xico: La Secretaria; 2001.
• ¹⁶
  Oteo JA, Nava S, Sousa R, Mattar S, Venzal JM, Abarca K, et al. Guías Latinoamericanas de la RIICER para el diagnóstico de las rickettsiosis transmitidas por garrapatas. Rev Chilena Infectol. 2014;31:54-65.
• ¹⁷
  Williams SG, Sacci JB Jr, Schriefer ME, Andersen EM, Fujioka KK, Sorvillo FJ, et al. Typhus and typhus-like Rickettsiae associated with opossums and their fleas in Los Angeles County, California. J Clin Microbiol. 1992;30:1758-62.
• ¹⁸
  Ruiz-Piña H, Reyes-Novelo E. El huerto familiar yucateco y las zoonosis. In: Flores JS, editor. Huertos familiares de la Península de Yucatán. Mérida: Universidad Autónoma de Yucatán; 2012. p. 359-74.
• ¹⁹
  Ruiz-Piña HA, Cruz-Reyes A. The opossum Didelphis virginiana as a synanthropic reservoir of Trypanosoma cruzi in Dzidzilche, Yucatan, Mexico. Mem Inst Oswaldo Cruz. 2002;97:613-20.
• ²⁰
  Ruiz-Piña HA, Puc-Franco MA, Flores-Abuxapqui J, Vado-Solis I, Cardenas-Marrufo MF. Isolation of Salmonella enterica and serologic reactivity to Leptospira interrogans in opossums (Didelphis virginiana) from Yucatan, Mexico. Rev Inst Med Trop Sao Paulo. 2002;44:235-7.
• ²¹
  Piranda EM, Faccini JL, Pinter A, Saito TB, Pacheco RC, Hagiwara MK, et al. Experimental infection of dogs with a Brazilian strain of Ricketssia ricketsii: clinical and laboratory findings. Mem Ins Oswaldo Cruz. 2008;103:696-701.
• ²²
  Reif KE, Macaluso KR. Ecology of Rickettsia felis: a review. J Med Entomol. 2009;46:723-36.

Publication Dates

• Publication in this collection
  2016

History

• Received
  09 Dec 2014
• Accepted
  06 Nov 2015