Resumo
Gametogenic and histochemical aspects of an edible bivalve, lphigenia brasiliana (Lamarek, 1818) from Itaipu Lagoon, Niterói, State of Rio de Janeiro, were studied. Between May 1998 and October 1999 a total of 120 clams were picked up. Samples of 10 animals were collected once a month. Specimens were fixed in Bouin's fixative for 4 hours or less and transferred to 10% formalin for 24 hours. The shell lenght has been measured with the valves lightly opened to permit the penetration of fixative solution deeply in the tissues. Transverse sections including digestive diverticula, mantle, gills, gonad and foot were obtained from the total body. After fixative treatment, tissues sections were embedded in paraffin, cut routinely at 5 µm and stained with Harris's hematoxylin and eosin. Microscopic analysis was carried out at low and high dry magnifications, as well as under oil immersion. Advanced ova stages were measured. Special staining techniques have been applied as a tentative tool for searching glycogen, mucous cells, collagenic and reticulin fibers, RNA and protozoan organisms. The animals collected showed variation in lenght from 7.20 cm to 4.10 cm and in height from 4.60 cm to 3.10 cm. The sex ratio (M:F) was 40:69. The average ova diameter of I. brasiliana was 34.70 µm with a standard deviation of 5.19 µm. Nothing could be observed in I. brasiliana, concerning to lesions and parasites at histopathological analysis.
Gametogenic cycle; histology; special-staining methods; Iphigenia brasiliana; ltaipu Lagoon; Brazil
Gametogenic cycle; histology; special-staining methods; Iphigenia brasiliana; ltaipu Lagoon; Brazil
Aspectos gametogênicos e histoquímicos de Iphigenia brasiliana (Lamarck) (Bivalvia, Donacidae) da Lagoa de Itaipu, Niterói, Rio de Janeiro, Brasil
Gametogenic and histochemical aspects of edible bivalve Iphigenia brasiliana (Lamarck) (Bivalvia, Donacidae) from the Itaipu lagoon, Niterói, Rio de Janeiro, Brazil
Eliana de Fátima Marques de MesquitaI,II; Marta Guimarães de AbreuIII; Francisco Carlos de LimaI
IDepartamento de Tecnologia de Alimentos, Universidade Federal Fluminense. Rua Dr. Vital Brazil Filho 64,24230-340 Niterói, Rio de Janeiro, Brasil. E-mail: eliana@esquadro.com.br
IIBolsista do CNPq
IIIBolsista de Iniciação Científica da FAPERJ/UFF
ABSTRACT
Gametogenic and histochemical aspects of an edible bivalve, lphigenia brasiliana (Lamarek, 1818) from Itaipu Lagoon, Niterói, State of Rio de Janeiro, were studied. Between May 1998 and October 1999 a total of 120 clams were picked up. Samples of 10 animals were collected once a month. Specimens were fixed in Bouin's fixative for 4 hours or less and transferred to 10% formalin for 24 hours. The shell lenght has been measured with the valves lightly opened to permit the penetration of fixative solution deeply in the tissues. Transverse sections including digestive diverticula, mantle, gills, gonad and foot were obtained from the total body. After fixative treatment, tissues sections were embedded in paraffin, cut routinely at 5 µm and stained with Harris's hematoxylin and eosin. Microscopic analysis was carried out at low and high dry magnifications, as well as under oil immersion. Advanced ova stages were measured. Special staining techniques have been applied as a tentative tool for searching glycogen, mucous cells, collagenic and reticulin fibers, RNA and protozoan organisms. The animals collected showed variation in lenght from 7.20 cm to 4.10 cm and in height from 4.60 cm to 3.10 cm. The sex ratio (M:F) was 40:69. The average ova diameter of I. brasiliana was 34.70 µm with a standard deviation of 5.19 µm. Nothing could be observed in I. brasiliana, concerning to lesions and parasites at histopathological analysis.
Key words: Gametogenic cycle, histology, special-staining methods, Iphigenia brasiliana, ltaipu Lagoon, Brazil
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Recebido em 02.03.2000; aceito em 30.V.2001.
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Datas de Publicação
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Publicação nesta coleção
04 Maio 2009 -
Data do Fascículo
Jun 2001
Histórico
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Aceito
30 Maio 2001 -
Recebido
02 Mar 2000
