Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos

Milagres, Lucimar Gonçalves; Melles, Carmo E.A.

doi:10.1590/S0036-46651992000400008

Resumos

S. saprophyticus é freqüentemente isolado de infecções do trato urinário de mulheres jovens e sexualmente ativas. Ao contrário de S. aureus, esta espécie não possui fatores de virulência bem definidos. O objetivo deste estudo é analisar a aderência de S. saprophyticus a células HEp-2 e eritrócitos de carneiro. As amostras foram isoladas a partir da urina de pacientes com infecção urinária. Foram realizados testes de hemaglutinação, aderência a células HEp-2 e a capacidade de carboidratos específicos inibirem as interações entre estes tipos celulares e S. saprophyticus. A maioria das cepas se mostrou hemaglutinante e sensível a inibição da hemaglutinação pela manose (100mM). Foram verificados altos níveis de aderência às células HEp-2. As diferenças em especificidade e nível de aderência do microrganismo a células de HEp-2 e eritrócitos sugerem a participação de diferentes adesinas nos processos de interações celulares.

Staphylococcus saprophyticus; Aderência; Células HEp-2; Hemaglutinação

S. saprophyticus has been frequently isolated from urinary tract infections in young women. In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species. The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes. The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed. Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction.

ARTIGOS ORIGINAIS

Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos

Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes

Lucimar Gonçalves Milagres^I; Carmo E.A. Melles^II

^IBiologista da Seção de Bacteriologia do Instituto Adolfo Lutz, São Paulo, SP, Brasil

^IIPesquisador Científico Nível VI da Seção de Bacteriologia do Instituto Adolfo Lutz. São Paulo, SP, Brasil

^{Endereço para correspondência} Endereço para correspondência: Instituto Adolfo Lutz, Seção de Bacteriologia 9º andar Av. Dr. Arnaldo, 351 01246-000 São Paulo, SP, Brasil

RESUMO

S. saprophyticus é freqüentemente isolado de infecções do trato urinário de mulheres jovens e sexualmente ativas. Ao contrário de S. aureus, esta espécie não possui fatores de virulência bem definidos.

O objetivo deste estudo é analisar a aderência de S. saprophyticus a células HEp-2 e eritrócitos de carneiro.

As amostras foram isoladas a partir da urina de pacientes com infecção urinária. Foram realizados testes de hemaglutinação, aderência a células HEp-2 e a capacidade de carboidratos específicos inibirem as interações entre estes tipos celulares e S. saprophyticus.

A maioria das cepas se mostrou hemaglutinante e sensível a inibição da hemaglutinação pela manose (100mM). Foram verificados altos níveis de aderência às células HEp-2. As diferenças em especificidade e nível de aderência do microrganismo a células de HEp-2 e eritrócitos sugerem a participação de diferentes adesinas nos processos de interações celulares.

Unitermos:Staphylococcus saprophyticus; Aderência; Células HEp-2; Hemaglutinação.

SUMMARY

S. saprophyticus has been frequently isolated from urinary tract infections in young women.

In contrast with S. aureus, no defined virulence factors have been recognized for the coagulase negative Staphylococcus species.

The objective this study was to analyze the adherence of S. saprophyticus to HEp-2 cells and sheep erythrocytes.

The sample were isolated from urine of patients with urinary infection. Hemagglutination, adherence to HEp-2 cells tests and inhibition by specific carbohydrates of the interactions between these cells were analyzed.

Most of the strains were hemagglutinanting whose properties was inhibited by mannose (100mM). There was a high adherence level to HEp-2 cells. The differences in specificity and attachment level noted in this study suggest that multiple adhesins are involved in the mechanism of cellular interaction.

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AGRADECIMENTOS

Este trabalho recebeu auxílio financeiro do CNPq. Processo nº 412516/88.

Agradecemos ao Sr. Murilo Gomes de Oliveira pela orientação recebida na elaboração deste trabalho e às Seções de Bacteriologia e Cultura Celular do Instituto Adolfo Lutz pelos serviços prestados.

Recebido para publicação em 06/06/1991

Aceito para publicação em 22/05/1992

1. ARP, L.H. - Bacterial infection of mucosal surfaces: an overview of cellular and molecular mechanisms. In: ROTH, S.A., ed. Virulence Mechanisms of bacterial pathogens. Washington, American Society of Microbiology. 1989. p. 3-19.
2. BASTOS, M.G.; MILAGRES, L.G.; OLIVEIRA, M.G. & PIMENTEL A.L. - Infecçăo urinária por S. saprophyticus I. Estudo da fase aguda. In: Congresso Brasileiro de Nefrologia, 13, Belo Horizonte, 1987. RESUMOS. p. 34.
3. BEACHEY, E.H. - Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J. infect. Dis., 143: 325-345, 1981.
4. BEUTH, J.; KO, H.L.; OHSHIMA, Y.; YASSIN, A.; UHLENBRUCK, G. & PULVERER, G. - The role of lectins and lipoteichoic acid in adherence of S. saprophyticus. Zbl. Bakt. Mikrobiol. Hyg., 268A: 357-361, 1988.
5. COLLEEN, S.; HOVELIUS, B.; WISLANDER, A. & MARDH, P.A. - Surface properties of S. saprophyticus and S. epidermidis as studied by adherence tests and two-polymer, aqueous phase systems. Acta path. microbiol. immunol. scand., 87B: 321-328, 1979.
6. COLLEEN, S.; HERRSTROM, P.; WIESLANDER, A. & MARDH, P.A. - Physico-chemical properties of S. epidermidis and S. saprophyticus as studied by aqueous polymer two-phase systems. Scand. J. infect. Dis., 24 (suppl.): 165-172, 1980.
7. COLLEEN, S. - Cell surface charge of human urethral mucosa in relation to bacterial attachment. Scand. J. Urol. Nephrol., 68(suppl.): 1-8, 1982.
8. COLLEEN, S.; MYHRBERG, H. & MARDH, P.A. - Bacterial colonization of human urethral mucosa. II - adherence tests using tissue organ cultures. Scand. J. Urol. Nephrol., 15: 181-187, 1981.
9. COSTERTON, J.W. & IRVIN, R.T. - The bacterial glycocalyx in nature and disease. Ann. Rev. Microbiol., 35: 299-324, 1981.
10. DARFEUILLE-MICHAUD, A.; FORESTIER, C.; JOLY, B. & CLUZEL, R. - Identification of a nonfimbrial adhesive factor of an enterotoxigenic E. coli strain. Infect. Immun., 52: 468-475, 1986.
11. GILLESPIE, W.A.; SELLIN, M.A.; GILL, P.; STEPHENS, M.; TUCKWELL, L.A. & HILTON, A.L. - Urinary tract infection in young women, with special reference to S. saprophyticus. J. din. Path., 31: 348-350, 1978.
12. GILSDORF, J.R.; JUDD, W.J. & CINAT, M. - Relationship of H. influenzae type b pilus structure and adherence to human erythrocytes. Infect. Immun., 57: 3259-3260, 1989.
13. GOLLEDGE, C.L. - S. saprophyticus bacteremia. J. infect. Dis., 157: 215, 1989.
14. GUNNARSSON, A.; MARDH, P.A.; LUNDBLAD, A. & SVENSON, S. - Oligosaccharide structures mediating agglutination of sheep erythrocytes by S. saprophyticus. Infect. Immun., 45: 41-46, 1984.
15. HOVELIUS, B. & MARDH, P.A. - Haemagglutination by S. saprophyticus and other Staphylococcal species. Acta path. microbiol. immunol. scand., 87B: 45-50, 1979.
16. HOVELIUS, B. & MARDH, P.A. - S. saprophyticus as a common cause of urinary tract infections. Rev. infect. Dis., 6: 328-337, 1984.
¹⁷
INSTITUT PASTEUR. - Milieu et réactifs de laboratorie Pasteur. Paris, Institut Pasteur, 1978. p. 120, 141, 186.
18. KLOOS, W.E. & SHLEIFER, K.H. - Simplified scheme for routine identification of human Staphylococcus species. J. clin. Microbiol., 1: 82-88, 1975.
19. LATHAM, R.H.; RUNNING, K. & STAMM, W.E. - Urinary tract infections in young adult women caused by S. saprophyticus. J. Amer. med. Ass., 250: 3063-3066, 1983.
20. LEE, W.; CARPENTER, R.J.; PHILLIPS, L.E. & FARO, S. - Pyelonephritis and sepsis due to S. saprophyticus. J. infect. Dis., 155: 1079, 1987.
21. LEFFLER, H.; LOMBERG, H.; GOTSCKICH, E.; HAGBERG, L.; JODAL, U.; KORHONEN T.; SAMUELSON, B.F.; SCHOOLNICK, G. & SVANBORG-EDÉN, C. - Chemical and clinical urinary tract infection. Scand. J. infect. Dis., 33(suppl.): 46-51, 1982.
22. MAGNUSSON, K.E. - Hidrofobic interaction - a mechanism of bacterial binding. Scand. J. infect. Dis., 33(suppl.): 32-36, 1982.
23. MARDH, P.A.; COLLEEN, S. & HOVELIUS, B. - Attachment of bacteria to exfoliated cells from the urogenital tract. Invest. Urol., 16: 322-326, 1979.
24. MARDH, P.A.; HOVELIUS, B.; MELSEN, F. & MOLLER, B.R. - Experimental acute pyelonephritis in grivet monkeys, provoked by S. saprophyticus. Acta path. microbiol. immunol. scand., 88B: 225-230, 1980.
25. MARRIE, T.J.; KWAN, C.; NOBLE, M.A.; WEST, A. & DUFFIELD, C. - S. saprophyticus as a cause of urinary tract infections. J. clin. Microbiol., 16: 427-431, 1982.
26. MARRIE, T.J.; LAM, J. & COSTERTON, J.W. - Bacterial adhesion to uroepithelial cells: a morphological study. J. infect. Dis., 142: 239-246, 1980.
27. ORSKOV, I.; BIRCH-ANDERSON, A.; DUGUID, J.P.; STENDERUP, J. & ORSKOV, F. - An adhesive protein capsule of E. coli. Infect. Immun., 47: 191-200, 1985.
28. PEAD, L.; MASKELL, R. & MORRIS, J. - S. saprophyticus a urinary pathogen: a six year prospective survey. Brit. med. J., 219: 1157-1159, 1985.
29. REID, G. & SOBEL, J.D. - Bacterial adherence in the pathogenesis of urinary tract infection: a review. Rev. infect. Dis., 9: 470-486, 1987.
30. SANSONETTI, P.J.; HALE, T.L.; DAMMIN, G.J.; KAPFER, C.; COLLINS, H.H.Jr & FORMAL, S.B. - Alterations in the pathogenicity of E. coli k-12 after transfer of plasmid and chromosomal genes from Shigella flexneri. Infect. Immun., 39: 1392-1402, 1983.
31. SCALETSKY, I.C.A.; SILVA, M.L.M. & TRABULSI, L.R. - Distinctive pattern of adherence of enteropathogenic E. coli to HeLa cells. Infect. Immun., 45: 534-536, 1984.
32. SCHIMIDT, H.; BUKHOLM, G. & HOLBERG-PETERSEN, M.H. - Adhesiveness and invasive ness of Staphylococcal species in a cell culture model. Acta path. microbiol. immunol., 97: 655-660, 1986.
33. SCHIMIDT, H.; NAUMANN, G. & PUTZKE, H.P. - Detection of different fimbriae-like structures on the surface of Staphylococcus saprophyticus. Zbl. Bakt. Mikrobiol. Hyg., 268A: 228-237, 1988.
34. STULL, T.L.; MENDELMAN. J.E.H.; HAAS, J.E.; SCHOENBORN, M.A.; MACK, K.D. & SMITH, A.L. - Characterization of Haemophilus influenzae type b fimbriae. Infect. Immun., 46: 787-796, 1984.
35. SVANBORG-EDÉN, C. & HANSON, L.A. - E. coli pili as possible mediators of attachment to urinary tract epthelial cells. Infect. Immun., 21: 229-237, 1978.
36. TETI, G.; CHIOFALO, M.S.; TOMASELLO, F.; FAVA, C. & MASTROENI, P. - Mediation of S. saprophyticus adherence to uroepithelial cells by lipoteichoic acid. Infect. Immun., 55: 839-842, 1987.
37. VVALLMARK, G.; ARREMARK, I. & TELANDER, B. - S. saprophyticus a frequent cause of acute urinary tract infection among female outpatients. J. infect. Dis., 138: 791-797, 1978.
38. WATHNE, B.; HOVELIUS, B. & MARDH, P.A. - Causes of frequency and dysuria in women. Scand. J. infect. Dis., 19: 223-229, 1987.

Endereço para correspondência:

Instituto Adolfo Lutz, Seção de Bacteriologia 9º andar

Av. Dr. Arnaldo, 351

01246-000 São Paulo, SP, Brasil

Datas de Publicação

Publicação nesta coleção
12 Set 2006
Data do Fascículo
Ago 1992

Histórico

Aceito
22 Maio 1992
Recebido
06 Jun 1991

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. ARP, L.H. - Bacterial infection of mucosal surfaces: an overview of cellular and molecular mechanisms. In: ROTH, S.A., ed. Virulence Mechanisms of bacterial pathogens. Washington, American Society of Microbiology. 1989. p. 3-19.

[2] 2. BASTOS, M.G.; MILAGRES, L.G.; OLIVEIRA, M.G. & PIMENTEL A.L. - Infecçăo urinária por S. saprophyticus I. Estudo da fase aguda. In: Congresso Brasileiro de Nefrologia, 13, Belo Horizonte, 1987. RESUMOS. p. 34.

[3] 3. BEACHEY, E.H. - Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces. J. infect. Dis., 143: 325-345, 1981.

[4] 4. BEUTH, J.; KO, H.L.; OHSHIMA, Y.; YASSIN, A.; UHLENBRUCK, G. & PULVERER, G. - The role of lectins and lipoteichoic acid in adherence of S. saprophyticus. Zbl. Bakt. Mikrobiol. Hyg., 268A: 357-361, 1988.

[5] 5. COLLEEN, S.; HOVELIUS, B.; WISLANDER, A. & MARDH, P.A. - Surface properties of S. saprophyticus and S. epidermidis as studied by adherence tests and two-polymer, aqueous phase systems. Acta path. microbiol. immunol. scand., 87B: 321-328, 1979.

[6] 6. COLLEEN, S.; HERRSTROM, P.; WIESLANDER, A. & MARDH, P.A. - Physico-chemical properties of S. epidermidis and S. saprophyticus as studied by aqueous polymer two-phase systems. Scand. J. infect. Dis., 24 (suppl.): 165-172, 1980.

[7] 7. COLLEEN, S. - Cell surface charge of human urethral mucosa in relation to bacterial attachment. Scand. J. Urol. Nephrol., 68(suppl.): 1-8, 1982.

[8] 8. COLLEEN, S.; MYHRBERG, H. & MARDH, P.A. - Bacterial colonization of human urethral mucosa. II - adherence tests using tissue organ cultures. Scand. J. Urol. Nephrol., 15: 181-187, 1981.

[9] 9. COSTERTON, J.W. & IRVIN, R.T. - The bacterial glycocalyx in nature and disease. Ann. Rev. Microbiol., 35: 299-324, 1981.

[10] 10. DARFEUILLE-MICHAUD, A.; FORESTIER, C.; JOLY, B. & CLUZEL, R. - Identification of a nonfimbrial adhesive factor of an enterotoxigenic E. coli strain. Infect. Immun., 52: 468-475, 1986.

[11] 11. GILLESPIE, W.A.; SELLIN, M.A.; GILL, P.; STEPHENS, M.; TUCKWELL, L.A. & HILTON, A.L. - Urinary tract infection in young women, with special reference to S. saprophyticus. J. din. Path., 31: 348-350, 1978.

[12] 12. GILSDORF, J.R.; JUDD, W.J. & CINAT, M. - Relationship of H. influenzae type b pilus structure and adherence to human erythrocytes. Infect. Immun., 57: 3259-3260, 1989.

[13] 13. GOLLEDGE, C.L. - S. saprophyticus bacteremia. J. infect. Dis., 157: 215, 1989.

[14] 14. GUNNARSSON, A.; MARDH, P.A.; LUNDBLAD, A. & SVENSON, S. - Oligosaccharide structures mediating agglutination of sheep erythrocytes by S. saprophyticus. Infect. Immun., 45: 41-46, 1984.

[15] 15. HOVELIUS, B. & MARDH, P.A. - Haemagglutination by S. saprophyticus and other Staphylococcal species. Acta path. microbiol. immunol. scand., 87B: 45-50, 1979.

[16] 16. HOVELIUS, B. & MARDH, P.A. - S. saprophyticus as a common cause of urinary tract infections. Rev. infect. Dis., 6: 328-337, 1984.

[17] ¹⁷
INSTITUT PASTEUR. - Milieu et réactifs de laboratorie Pasteur. Paris, Institut Pasteur, 1978. p. 120, 141, 186.

[18] 18. KLOOS, W.E. & SHLEIFER, K.H. - Simplified scheme for routine identification of human Staphylococcus species. J. clin. Microbiol., 1: 82-88, 1975.

[19] 19. LATHAM, R.H.; RUNNING, K. & STAMM, W.E. - Urinary tract infections in young adult women caused by S. saprophyticus. J. Amer. med. Ass., 250: 3063-3066, 1983.

[20] 20. LEE, W.; CARPENTER, R.J.; PHILLIPS, L.E. & FARO, S. - Pyelonephritis and sepsis due to S. saprophyticus. J. infect. Dis., 155: 1079, 1987.

[21] 21. LEFFLER, H.; LOMBERG, H.; GOTSCKICH, E.; HAGBERG, L.; JODAL, U.; KORHONEN T.; SAMUELSON, B.F.; SCHOOLNICK, G. & SVANBORG-EDÉN, C. - Chemical and clinical urinary tract infection. Scand. J. infect. Dis., 33(suppl.): 46-51, 1982.

[22] 22. MAGNUSSON, K.E. - Hidrofobic interaction - a mechanism of bacterial binding. Scand. J. infect. Dis., 33(suppl.): 32-36, 1982.

[23] 23. MARDH, P.A.; COLLEEN, S. & HOVELIUS, B. - Attachment of bacteria to exfoliated cells from the urogenital tract. Invest. Urol., 16: 322-326, 1979.

[24] 24. MARDH, P.A.; HOVELIUS, B.; MELSEN, F. & MOLLER, B.R. - Experimental acute pyelonephritis in grivet monkeys, provoked by S. saprophyticus. Acta path. microbiol. immunol. scand., 88B: 225-230, 1980.

[25] 25. MARRIE, T.J.; KWAN, C.; NOBLE, M.A.; WEST, A. & DUFFIELD, C. - S. saprophyticus as a cause of urinary tract infections. J. clin. Microbiol., 16: 427-431, 1982.

[26] 26. MARRIE, T.J.; LAM, J. & COSTERTON, J.W. - Bacterial adhesion to uroepithelial cells: a morphological study. J. infect. Dis., 142: 239-246, 1980.

[27] 27. ORSKOV, I.; BIRCH-ANDERSON, A.; DUGUID, J.P.; STENDERUP, J. & ORSKOV, F. - An adhesive protein capsule of E. coli. Infect. Immun., 47: 191-200, 1985.

[28] 28. PEAD, L.; MASKELL, R. & MORRIS, J. - S. saprophyticus a urinary pathogen: a six year prospective survey. Brit. med. J., 219: 1157-1159, 1985.

[29] 29. REID, G. & SOBEL, J.D. - Bacterial adherence in the pathogenesis of urinary tract infection: a review. Rev. infect. Dis., 9: 470-486, 1987.

[30] 30. SANSONETTI, P.J.; HALE, T.L.; DAMMIN, G.J.; KAPFER, C.; COLLINS, H.H.Jr & FORMAL, S.B. - Alterations in the pathogenicity of E. coli k-12 after transfer of plasmid and chromosomal genes from Shigella flexneri. Infect. Immun., 39: 1392-1402, 1983.

[31] 31. SCALETSKY, I.C.A.; SILVA, M.L.M. & TRABULSI, L.R. - Distinctive pattern of adherence of enteropathogenic E. coli to HeLa cells. Infect. Immun., 45: 534-536, 1984.

[32] 32. SCHIMIDT, H.; BUKHOLM, G. & HOLBERG-PETERSEN, M.H. - Adhesiveness and invasive ness of Staphylococcal species in a cell culture model. Acta path. microbiol. immunol., 97: 655-660, 1986.

[33] 33. SCHIMIDT, H.; NAUMANN, G. & PUTZKE, H.P. - Detection of different fimbriae-like structures on the surface of Staphylococcus saprophyticus. Zbl. Bakt. Mikrobiol. Hyg., 268A: 228-237, 1988.

[34] 34. STULL, T.L.; MENDELMAN. J.E.H.; HAAS, J.E.; SCHOENBORN, M.A.; MACK, K.D. & SMITH, A.L. - Characterization of Haemophilus influenzae type b fimbriae. Infect. Immun., 46: 787-796, 1984.

[35] 35. SVANBORG-EDÉN, C. & HANSON, L.A. - E. coli pili as possible mediators of attachment to urinary tract epthelial cells. Infect. Immun., 21: 229-237, 1978.

[36] 36. TETI, G.; CHIOFALO, M.S.; TOMASELLO, F.; FAVA, C. & MASTROENI, P. - Mediation of S. saprophyticus adherence to uroepithelial cells by lipoteichoic acid. Infect. Immun., 55: 839-842, 1987.

[37] 37. VVALLMARK, G.; ARREMARK, I. & TELANDER, B. - S. saprophyticus a frequent cause of acute urinary tract infection among female outpatients. J. infect. Dis., 138: 791-797, 1978.

[38] 38. WATHNE, B.; HOVELIUS, B. & MARDH, P.A. - Causes of frequency and dysuria in women. Scand. J. infect. Dis., 19: 223-229, 1987.

Brasil

Brasil

Diferenças nas propriedades adesivas de Staphylococcus saprophyticus a células HEp-2 e eritrócitos

Differences on the adhesive property of Staphylococcus saprophyticus to HEp-2 cells and erythrocytes

Resumos

Endereço para correspondência:

Datas de Publicação

Histórico