Species and serovars of enteropathogenic agents associated with acute diarrheal disease in Rosario, Argentina

Notario, Rodolfo; Borda, Noemi; Gambande, Telma; Sutich, Emma

doi:10.1590/S0036-46651996000100002

Abstracts

We report the most frequent species and serovars of enteropathogenic organisms in Rosario from 1985 to 1993. Enteropathogenic Escherichia coli was the most prevalent agent affecting 144/570 (25.2%) children; 0111 represented 41.8%, 055: 13.6%, 0119: 12.7%. Among enterotoxigenic E. coli (ETEC) the most frequent were ETEC-ST 0128:H21 and 0153:H45. Shigella spp were isolated in 8.8%; S.flexneri: 7%, principally type 2 (59.5%); S. sonnei: 1.6%, and S. dysenteriae type 2: 0.2%. Campylobacter spp were found in 6.1% of patients; C.jejuni: 4.6%; C. coli: 1.4% and C. lari: 0.2%; except groups 0 13,50 and 0 4 (2 cases each), no predominant serogroups were found. Salmonella was isolated in 2.8% of cases, being the predominant serovar S. typhimurium until 1986, but a dramatically increase of cases due to S. enteritidis was observed since 1987. There was 1.9% of Aeromonas spp and 2 cases due to Vibrio cholerae non 0-1. No Yersinia was found. In patients with gastroenteritis due to Shigella, Campylobacter, Salmonella, or EPEC as the unique pathogen, leukocytes were observed in the faeces in 70%, 50%, 20%, and 10% of cases respectively.

Acute diarrhea; Enteropathogenic agents-species; Serovars; Rosario; Argentina

Apresentamos as espécies e serovars mais frequentes dos microorganismos enteropatógenos entre 1985 e 1990 em Rosario. Escherichia coli enteropatogênica (EPEC) foi a que predominou, afetando 144/570 (25,2%) crianças; 0111 representou 41,8%, 055 13,6%, 0119 12,7%. Entre as E. coli enterotoxigênicas (ETEC), ETEC-ST 0128:H21 e 0153:H45 foram as mais frequentes. Entre os 570 pacientes, Shigella spp. foi diagnosticada em 50 (8,8%); S. flexneri 7%, principalmente do tipo 2 (59,5%), S. sonnei 1,6% e S. dysenteriae tipo 2 (1%). Foram encontrados Campylobacter spp em 6,1% dos pacientes; C. jejuni 4,6%, C. coli 1,4% e C. lari 0,2%; exceto os grupos 0 13/50 e 0 4 (dois de cada um), não foram encontrados serogrupos predominantes. Salmonella foi encontrada em 2,8% dos casos, sendo o serovar S. typhimurium o predominante até 1986, mas desde 1987, foi observado um aumento importante de casos por S. enteritidis. Houve 1,9% de Aeromonas spp e dois casos por Vibrio cholerae não 01. Não se encontrou Yersinia spp. Nos pacientes com gastroenteritis por Shigella, Campylobacter, Salmonella e EPEC como único patógeno, foram observados leucocitos nas feses de 70%, 50%, 20% e 10% dos casos respectivamente.

MICROBIOLOGY

Species and serovars of enteropathogenic agents associated with acute diarrheal disease in Rosario, Argentina

Espécies e serovariantes de agentes enteropatogênicos associados com diarréia aguda em Rosario, Argentina

Rodolfo Notario^I; Noemi Borda^II; Telma Gambande^II; Emma Sutich^III

^ICátedra de Microbiología, Virología y Parasitología. Facultad de Ciencias Médicas. Universidad Nacional de Rosario, Argentina

^IIInstituto de Diagnóstico ABC

^IIICentro Médico IPAM

^{Correspondence to} Correspondence to: Prof. Dr. Rodolfo Notario Uriburu 918 2000 Rosario, Argentina

SUMMARY

We report the most frequent species and serovars of enteropathogenic organisms in Rosario from 1985 to 1993. Enteropathogenic Escherichia coli was the most prevalent agent affecting 144/570 (25.2%) children; 0111 represented 41.8%, 055: 13.6%, 0119: 12.7%. Among enterotoxigenic E. coli (ETEC) the most frequent were ETEC-ST 0128:H21 and 0153:H45. Shigella spp were isolated in 8.8%; S.flexneri: 7%, principally type 2 (59.5%); S. sonnei: 1.6%, and S. dysenteriae type 2: 0.2%. Campylobacter spp were found in 6.1% of patients; C.jejuni: 4.6%; C. coli: 1.4% and C. lari: 0.2%; except groups 0 13,50 and 0 4 (2 cases each), no predominant serogroups were found. Salmonella was isolated in 2.8% of cases, being the predominant serovar S. typhimurium until 1986, but a dramatically increase of cases due to S. enteritidis was observed since 1987. There was 1.9% of Aeromonas spp and 2 cases due to Vibrio cholerae non 0-1. No Yersinia was found. In patients with gastroenteritis due to Shigella, Campylobacter, Salmonella, or EPEC as the unique pathogen, leukocytes were observed in the faeces in 70%, 50%, 20%, and 10% of cases respectively.

Keywords: Acute diarrhea; Enteropathogenic agents-species; Serovars; Rosario, Argentina.

RESUMO

Apresentamos as espécies e serovars mais frequentes dos microorganismos enteropatógenos entre 1985 e 1990 em Rosario. Escherichia coli enteropatogênica (EPEC) foi a que predominou, afetando 144/570 (25,2%) crianças; 0111 representou 41,8%, 055 13,6%, 0119 12,7%. Entre as E. coli enterotoxigênicas (ETEC), ETEC-ST 0128:H21 e 0153:H45 foram as mais frequentes. Entre os 570 pacientes, Shigella spp. foi diagnosticada em 50 (8,8%); S. flexneri 7%, principalmente do tipo 2 (59,5%), S. sonnei 1,6% e S. dysenteriae tipo 2 (1%). Foram encontrados Campylobacter spp em 6,1% dos pacientes; C. jejuni 4,6%, C. coli 1,4% e C. lari 0,2%; exceto os grupos 0 13/50 e 0 4 (dois de cada um), não foram encontrados serogrupos predominantes.

Salmonella foi encontrada em 2,8% dos casos, sendo o serovar S. typhimurium o predominante até 1986, mas desde 1987, foi observado um aumento importante de casos por S. enteritidis. Houve 1,9% de Aeromonas spp e dois casos por Vibrio cholerae não 01. Não se encontrou Yersinia spp. Nos pacientes com gastroenteritis por Shigella, Campylobacter, Salmonella e EPEC como único patógeno, foram observados leucocitos nas feses de 70%, 50%, 20% e 10% dos casos respectivamente.

Full text available only in PDF format.

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Recebido para publicação em 19/10/1994.

Aceito para publicação em 09/02/1996.

1. BINSZTEIN, N.; JOUVE, M.J.; VIBOUD, G.I. et al. - Colonization factors of enterotoxigenic Escherichia coli isolated from children with diarrhea in Argentina. J. clin. Microbiol., 29: 1893-1898, 1991.
2. BLANCO, J.; GONZALEZ, E.A.; BLANCO, M. et al. - Prevalence of enterotoxigenic Escherichia coli strains in outbreaks and sporadic cases of diarrhea in Spain. Europ. J. clin. Microbiol. infect. Dis., 8: 396-400, 1989.
3. EIGUER, T.; CAFFER, M.I. & FRONCHKOWSKY, G.B. - Importancia de la Salmonella enteritidis en brotes de enfermedades transmitidas por alimentos en Argentina, ańos 1986-1988. Rev. argent. Microbiol., 22: 31-34, 1990.
4. GUERRANT, R.L.; SHIELDS, D.S.; THORSON, S.M. et al. - Evaluation and diagnosis of acute infectious diarrhea. Amer. J. Med., 78: 91-97, 1985.
5. NOTARIO, R.; BORDA, N.; DESERTI, S. & GAMBANDÉ, T. - Infecciones entéricas por Campylobacter jejuni en Rosario. Medicina (B. Aires), 45: 654-658, 1985.
6. NOTARIO, R.; BORDA, N.; MORALES, E. et al. - Dos casos de gastroenteritis debida a Vibrio Cholerae no 0-1 en Rosario. Infect. Microbiol. clin., 5: 123-125, 1993.
7. NOTARIO, R.; MORALES, E.; CARMELENGO, E. et al. - Microorganismos enteropatógenos en nińos con diarrea aguda en dos hospitales de Rosario, Argentina. Medicina (B. Aires), 53: 289-299, 1993.
8. NOTARIO, R.; PEREYRA, A.; GAMBANDE, T. & BORDA, N. - Biotipificación y serotipificación de Campylobacter jejuni, Campylobacter coli y Campylobacter lari. Infect. Microbiol. clin., 5: 60-65, 1993.
9. NOTARIO, R.; SUTICH, E.G. & BORDA, N.G. - Primeros casos debidos a Shigella dysenteriae tipo 2 en Rosario. Acta bioquim. clin. lat.-amer., 17: 553-560, 1983.
10. PENNER, J.L. & HENNESY, J.N. - Passive hemagglutination technique for serotyping Campylobacter fetus subsp jejuni on the basis of soluble heatstable antigens. J. clin. Microbiol., 12: 732-737, 1980.
11. PRADO, V.; SIRI, M.T.; AVENDAŃO, L.F. et al. - Prevalencia de agentes enteropatógenos en síndrome diarreico agudo en nińos hospitalizados y ambulatorios. Rev. chil. Pediat., 58: 285-290, 1987.
12. TARDELLI GOMEZ, T.A.; RASSI, V.; MAC DONNALD, K.L. et al. - Enteropathogens associated with acute diarrheal disease in urban infants in Sao Paulo, Brazil. J. infect. Dis., 164: 331-337, 1991.
13. TRABULSI, L.R.; TOLEDO, M.R.F.; MURAHOVSCHI, J. et al. - Epidemiology of infantile bacterial diarrheal disease in Brazil. In: TAKEDA, Y. & MIWATANI, T., ed. Bacterial diarrheal diseases. Tokyo, K. T. K. Scientific Publishers, 1985. p. 25.
¹⁴
WORLD HEALTH ORGANIZATION - Programme for control of diarrheal diseases. Geneva, WHO, Interim Programme Report, 1990. (WHO/CDD/91).

Correspondence to:

Prof. Dr. Rodolfo Notario

Uriburu 918

2000 Rosario, Argentina

Publication Dates

Publication in this collection
22 Sept 2006
Date of issue
Feb 1996

History

Received
19 Oct 1994
Accepted
09 Feb 1996

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. BINSZTEIN, N.; JOUVE, M.J.; VIBOUD, G.I. et al. - Colonization factors of enterotoxigenic Escherichia coli isolated from children with diarrhea in Argentina. J. clin. Microbiol., 29: 1893-1898, 1991.

[2] 2. BLANCO, J.; GONZALEZ, E.A.; BLANCO, M. et al. - Prevalence of enterotoxigenic Escherichia coli strains in outbreaks and sporadic cases of diarrhea in Spain. Europ. J. clin. Microbiol. infect. Dis., 8: 396-400, 1989.

[3] 3. EIGUER, T.; CAFFER, M.I. & FRONCHKOWSKY, G.B. - Importancia de la Salmonella enteritidis en brotes de enfermedades transmitidas por alimentos en Argentina, ańos 1986-1988. Rev. argent. Microbiol., 22: 31-34, 1990.

[4] 4. GUERRANT, R.L.; SHIELDS, D.S.; THORSON, S.M. et al. - Evaluation and diagnosis of acute infectious diarrhea. Amer. J. Med., 78: 91-97, 1985.

[5] 5. NOTARIO, R.; BORDA, N.; DESERTI, S. & GAMBANDÉ, T. - Infecciones entéricas por Campylobacter jejuni en Rosario. Medicina (B. Aires), 45: 654-658, 1985.

[6] 6. NOTARIO, R.; BORDA, N.; MORALES, E. et al. - Dos casos de gastroenteritis debida a Vibrio Cholerae no 0-1 en Rosario. Infect. Microbiol. clin., 5: 123-125, 1993.

[7] 7. NOTARIO, R.; MORALES, E.; CARMELENGO, E. et al. - Microorganismos enteropatógenos en nińos con diarrea aguda en dos hospitales de Rosario, Argentina. Medicina (B. Aires), 53: 289-299, 1993.

[8] 8. NOTARIO, R.; PEREYRA, A.; GAMBANDE, T. & BORDA, N. - Biotipificación y serotipificación de Campylobacter jejuni, Campylobacter coli y Campylobacter lari. Infect. Microbiol. clin., 5: 60-65, 1993.

[9] 9. NOTARIO, R.; SUTICH, E.G. & BORDA, N.G. - Primeros casos debidos a Shigella dysenteriae tipo 2 en Rosario. Acta bioquim. clin. lat.-amer., 17: 553-560, 1983.

[10] 10. PENNER, J.L. & HENNESY, J.N. - Passive hemagglutination technique for serotyping Campylobacter fetus subsp jejuni on the basis of soluble heatstable antigens. J. clin. Microbiol., 12: 732-737, 1980.

[11] 11. PRADO, V.; SIRI, M.T.; AVENDAŃO, L.F. et al. - Prevalencia de agentes enteropatógenos en síndrome diarreico agudo en nińos hospitalizados y ambulatorios. Rev. chil. Pediat., 58: 285-290, 1987.

[12] 12. TARDELLI GOMEZ, T.A.; RASSI, V.; MAC DONNALD, K.L. et al. - Enteropathogens associated with acute diarrheal disease in urban infants in Sao Paulo, Brazil. J. infect. Dis., 164: 331-337, 1991.

[13] 13. TRABULSI, L.R.; TOLEDO, M.R.F.; MURAHOVSCHI, J. et al. - Epidemiology of infantile bacterial diarrheal disease in Brazil. In: TAKEDA, Y. & MIWATANI, T., ed. Bacterial diarrheal diseases. Tokyo, K. T. K. Scientific Publishers, 1985. p. 25.

[14] ¹⁴
WORLD HEALTH ORGANIZATION - Programme for control of diarrheal diseases. Geneva, WHO, Interim Programme Report, 1990. (WHO/CDD/91).

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