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Methods for field detection of resistance to temephos in simuliids. Larval esterase level and topical application of the insecticide to adults

Abstract

Two practical field methods for indirect detection of simuliid populations resistant to temephos are proposed. The first is based on high esterase activity in resistant larvae and involves adaptations of a filter paper test in which faintly stained spots indicate susceptible populations and strongly stained ones reveal populations resistant to temephos. The second is based on the resistance to the larvicide when adults are topically exposed, and involves the use of diagnostic doses obtained by the comparison between the LD50 for susceptible and resistant populations. The relevance of such methods is discussed in order to help resistance detection in Simulium pertinax Kollar control programmes.

Simuliidae; resistance detection methods; temephos; Simulium pertinax


ABSTRACT

Methods for field detection of resistance to temephos in simuliids. Larval esterase level and topical application of the insecticide to adults

Carlos Fernando S. de Andrade1

Armando Castello Branco Junior2

UNICAMP, Instituto de Biologia, Departamento de Zoologia, Campinas, Brasil

UNICAMP, Instituto de Biologia, Pós-Graduação em Ecologia, Campinas, Brasil

Two practical field methods for indirect detection of simuliid populations resistant to temephos are proposed. The first is based on high esterase activity in resistant larvae and involves adaptations of a filter paper test in which faintly stained spots indicate susceptible populations and strongly stained ones reveal populations resistant to temephos. The second is based on the resistance to the larvicide when adults are topically exposed, and involves the use of diagnostic doses obtained by the comparison between the LD50 for susceptible and resistant populations. The relevance of such methods is discussed in order to help resistance detection in Simulium pertinax Kollar control programmes.

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Publication Dates

  • Publication in this collection
    04 June 2009
  • Date of issue
    Sept 1990
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