Animal model of the abdominal compartment syndrome as a single insult and as a second insult in rats

Modelo experimental de síndrome de compartimento abdominal como insulto único e como um segundo insulto, em ratos

EXPERIMENTAL MODELS

Animal model of the abdominal compartment syndrome as a single insult and as a second insult in rats¹

Modelo experimental de síndrome de compartimento abdominal como insulto único e como um segundo insulto, em ratos

João Baptista de Rezende-Neto^I ; Alcino Lázaro da Silva^II; José Renan Cunha-Melo^II

^IAssistent Professor - Surgery Departament of Faculty of Medicine - Federal University of Minas Gerais.

^IIHead of Digestive Surgery - Surgery Departament of Faculty of Medicine - Federal University of Minas Gerais.

^{Correspondence to}

ABSTRACT: The objective of this paper was to develop a clinically relevant abdominal compartment syndrome experimental model, as a single insult and as a second insult flowing hemorrhagic shock. In the single insult model, Sprague-Dawley male-rats are anesthetized, invasively monitored (central venous pressure and mean arterial pressure), and mechanically ventilated during intraperitoneal injection of air to provoke the abdominal compartment syndrome (25 mmHg) for 60 minutes. In the two insult model, Sprague-Dawley male-rats are anesthetized, invasively monitored (mean arterial pressure) and bled to a mean arterial pressure of 30 mmHg for 45 minutes. Fluid resuscitation is accomplished by infusing 0.9% sodium chloride solution (0.9% NaCl) 33.2 ml/kg plus 75% of shed blood volume. During this phase a laparotomy is performed. Two hours after the beginning of the hemorrhagic shock phase the animals are anesthetized, intubated (orotracheal), mechanically ventilated (mean arterial pressure), and the intra-abdominal pressure is increased to 25 mmHg for 60 minutes, as a second insult. A 0.9% NaCl solution is infused during this phase (45 ml/kg/h). Hemorrhagic shock and the abdominal compartment syndrome behave as clinically relevant additive insults.

KEY WORDS: Animal model. abdominal compartment syndrome. Inflammatory response. Lung injury. Multiple organ failure.

RESUMO: Este artigo propõe o desenvolvimento de um modelo experimental, clinicamente relevante, de síndrome de compartimento abdominal como insulto único e como segundo insulto após choque hemorrágico. No modelo de insulto único, ratos Sprague-Dawley machos, anestesiados, sob monitorização invasiva da pressão venosa central, pressão arterial média e ventilação mecânica são submetidos a síndrome de compartimento abdominal (25 mmHg) por 60 minutos através da injeção de ar na cavidade peritoneal. No modelo de dois insultos ratos Sprague-Dawley machos, anestesiados, sob monitorização invasiva da pressão arterial média, são submetidos a choque hemorrágico (30 mmHg) por 45 minutos seguido de reposição volêmica com solução de cloreto de sódio a 0,9% (NaCl) 33,2 ml/kg mais 75% da sangria e laparotomia. Duas horas após o início do choque hemorrágico os animais são anestesiados, intubados por via orotraqueal, colocados em ventilação mecânica, monitorizados invasivamente (pressão arterial média) e submetidos à síndrome de compartimento abdominal. Nesta fase é administrada solução NaCl 0,9% a 45 ml/kg/h. A associação dos insultos, síndrome de compartimento abdominal e choque hemorrágico, proporciona modelo clinicamente relevante.

DESCRITORES: Modelo animal. Síndrome compartimento abdominal. Resposta inflamatória. Lesão pulmonar. Falência de múltiplos órgãos.

Introduction

The abdominal compartment syndrome (ACS), defined as an elevated intra-abdominal pressure at a sufficient level to produce an adverse physiologic response, is a known complication of traumatic and non-traumatic surgery and important confounder of surgical intensive care following trauma.^1,2,3,4,5 Respiratory derangement caused by elevated intra-abdominal pressure has been known since the late 1800’s, and the deleterious effects of intra-abdominal hypertension (IAH) on other organ systems were recognized in the early 1900’s.^6,7 To this date it has been demonstrated that practically all organs are affected by the ACS, except for the adrenal glands.^1,2,3 Although intra-abdominal pressure of 25-35 mmHg have been recognized as a potential for the development of ACS, there is still no consensus as to what is the critical intra-abdominal pressure.^1,2,3 In a recent clinical study of damage control laparotomy, early ACS emerged as an independent risk factor for postinjury multiple organ failure (MOF).⁸ Furthermore, hemorrhagic shock facilitates the occurrence of ACS. In the two-event model of MOF, the first insult primes the inflammatory cascade, creating a vulnerable window to secondary insults capable of activating the system and precipitating late MOF.⁹ It has recently been shown, in a rodent model, that the ACS induces the systemic release of pro-inflammatory cytokines (Interleukin (IL-1, IL-6, TNFa) which may become critical in serving as a second insult for the induction of post-injury MOF.¹⁰

Proposition

A limiting factor for advance in the pathogenesis of multiple organ failure (MOF) research is the lack of clinically relevant animal models capable of simulating on the bench the severe insults seen on clinical practice.¹⁰ To fulfill that necessity, two experimental models are described in this paper. The first model refers to the ACS as a single insult, whereas the second model was designed to simulate a critically ill hemorrhagic shock patient under volume resuscitation, surgical intervention (laparotomy) followed by a post-operative complication, the ACS. These models should serve not only to determine the physiologic derangement caused by the ACS but also as tools for further systemic inflammatory response syndrome research.

Method description - Abdominal Compartment Syndrome as a Single Insult

1 . Animals

Male Sprague-Dawley rats weighing between 250-300g

2. Anesthesia

Pentobarbital sodium, 50 mg/kg administered intraperitoneally.

3. Body Temperature

Rectal temperature is maintained between 37-38⁰C throughout the procedure with a heating lamp.

4. Skin Antisepsis

Surgical sites are prepared with antiseptic bactericidal solution.

5. Local Anesthesia

Surgical sites are injected with 1% lidocaine (0,5 ml).

6. Surgical Procedure

Under aseptic technique a 7 mm longitudinal cervical incision exposes the trachea which is lifted by two 4-0 silk stay sutures. A 1.5 cm long angiocath (14G) is introduced into the trachea through a 2 mm incision on the anterior aspect of the trachea. The right jugular vein is exposed through a 5 mm longitudinal cervical incision and cannulated with a previously heparinized polyethylene tubing (PE50) 10 cm long. The catheter is tied over the vein with stay sutures. The incision is closed with a single 5-0 nylon stitch. Access to the left common carotid artery is obtained using the same method described above.

7. Access to the Abdominal Cavity

The abdominal cavity is accessed at two different sites. Firstly an angiocath (20G) is positioned (percutaneous) into the abdominal cavity, on the lower third, to the right of the midline. Another catheter (angiocath 20G) is positioned in the same manner to the left of the midline. This catheter is connected to a 0.45 μm air filter and a three way intravenous injection device (Figure 1).

8. Monitoring

The right jugular vein catheter and the left carotid artery catheter are individually connected to a pressure transducer for continuous monitoring of the central venous pressure (CVP) and the mean arterial pressure (MAP). Data is shown on a personal computer screen using specific software. Intra-abdominal pressure is continuously assessed through the right intra-abdominal catheter connected to an individual pressure transducer. Baseline MAP, CVP and intra-abdominal pressure are assessed for 5 minutes before provoking the ACS.

9. Mechanical Ventilation

After baseline measurements the endotracheal catheter is connected to a rodent mechanical ventilator set for 60 breaths per minute and tidal volume of 1.5 ml/100g of body weight. New baseline values are obtained for 5 minutes. To assess the isolated effects of the ACS, no intravenous fluids are given to the animals in during the period of HIA

10. Intra-abdominal hypertension

Intra-abdominal pressure is initially increased to 10 mmHg by manual injection of room air, with a 20 ml syringe connected to the catheter on the midline. After 10 minutes more air in injected, in the same manner, until the final intra-abdominal pressure of 25 mmHg is achieved (Figure 2). Continuous hemodynamic monitoring is maintained throughout the procedure.

Method description - Two Insult Model (Hemorrhagic Shock Followed by the Abdominal Compartment Syndrome)

This model involved the same procedures previously described up to number 5.

1. Surgical procedure before hemorrhagic shock

The left jugular vein and the left femoral artery are cannulated with previously heparinized 10 cm long PE-50 catheters which are exteriorized through the back of the neck and thigh, respectively, using a 16G needle. This procedure is to prevent the animals from biting on the catheter.

2. Monitoring

Baseline MAP and blood gases are obtained and then MAP is continuously monitored throughout the hemorrhagic shock phase using a digital monitor.

3. Hemorrhagic shock

Hemorrhagic shock begins by the aspiration of 4 ml of blood from the femoral artery catheter. Additional volumes are aspirated every five minutes to lower the MAP to 30 mmHg. Mean arterial pressure is maintained at this level for 45 minutes. Shed blood is stored in an incubator at 37⁰C inside sterile tubes.

4. Volume resuscitation

The animals are resuscitated with 33,2 ml/kg of 0.9% sodium chloride (0.9% NaCl) solution plus 75% of the shed blood volume, mixed in a sterile tube. The total volume is infused in the left jugular vein catheter with an infusion pump during 60 minutes.

5. Surgical procedure after hemorrhagic shock

Ten minutes after beginning the resuscitation phase the animals undergo a 2 cm long midline laparotomy under aseptic technique. Two angiocatheters (14G) are placed through the abdominal wall, under direct vision, on each side of the midline (Figure 3). The catheters are occluded and anchored to the skin with a single 4-0 silk stitch each. A sterile seven inches latex balloon is placed inside the abdominal cavity (Figure 4). The open end of the balloon is exteriorized through a midline counter-incision at the most distal portion of the laparotomy and anchored to the skin with a single 4-0 silk stitch. The midline laparotomy and the skin are closed with interrupted 4-0 silk sutures.

6. Post-operative analgesia

After volume resuscitation the animals are placed in appropriate cages and receive food and water ad libitum. Acetaminophen is dissolved in water (0.1 mg/dose) for postoperative analgesia, 24 hours following surgery.

7. Anesthesia for intra-abdominal hypertension

Two hours after the beginning of the hemorrhagic shock phase the animals are again anesthetized as previously described.

8. Endotracheal intubation

The animals are placed on their backs with the neck in hyperextension. A light source is applied on the neck to visualize the tracheal lumen. The animal’s tongue is grasped with a forceps and pulled to the outside and a spatula is used to visualize the glottis where a 16G angiocatheter (1.5 cm long) is inserted under direct vision. The catheter is anchored to the central incisor with a 4-0 silk tie.

9 . Hemodynamic monitoring

The vascular catheters and the abdominal catheter left of the midline are connected to the monitor.

10. Mechanical ventilation

The animals are mechanically ventilated as previously described.

11. Intra-abdominal hypertension as the second insult

Initially 15 ml of air are added to the latex balloon with a device used to insufflate laparoscopic hernioplasty balloon. This air volume causes the latex balloon to occlude the laparotomy incision therefore preventing intra-abdominal air escape. Intra-abdominal pressure is increased by manual air injection, through the abdominal catheter positioned on the right side of the midline, by increments of 5 mmHg every 10 minutes up to 25 mmHg. This final pressure is maintained for 60 minutes. A 0.9% NaCl solution is continuously infused (45ml/kg/h) through the left jugular vein catheter during the intra-abdominal hypertension phase. The purpose for fluid infusion during this phase is to maintain MAP above or equal to 80 mmHg. This pressure is accepted as adequate endpoint in hemorrhagic shock resuscitation.¹¹

12. Ventilator wean after the second insult

Mechanical ventilation weaning and extubation are completed 10 minutes after abdominal decompression. The animals are placed in appropriate cages as previously described.

Perspectives

The lack of clinically relevant experimental models to study the relationship between trauma and the systemic inflammatory response syndrome (SIRS) jeopardizes this field of research. There are significant drawbacks in models that provoke SIRS by injections of bacterial components, toxins and other substances. Those approaches create an artificial environment that bypasses the staged development of SIRS, therefore loosing clinical significance. The experimental models described in this study provide adequate means to explore the immune consequences of the ACS and the systemic inflammatory response to other insults in a clinically relevant environment.

Conflict of interest: none

Finantial source: none

Data do recebimento: 22/ 04/2003

Data da revisão: 18/05/2003

Data da aprovação: 28/07/2003

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¹

Faculty of Medicine Federal University of Minas Gerais. Brazil.

Publication Dates

• Publication in this collection
  16 Jan 2004
• Date of issue
  2003

History

• Accepted
  28 July 2003
• Received
  22 Apr 2003
• Reviewed
  18 May 2003