Analysis method of intracellular iron accumulated in phytoplankton

Paredes, Jorge Falcão; Schmidt, Gilda

doi:10.1590/S0373-55241977000100001

Abstracts

A colorimetric method for the assessment of iron accumulated in the cells of phytoplankton utilizing potassium ferrocyanide was developed. Two experiences with Phaeodactylum tricornutum and Chlorella sp. were carried out: the first one, on uptake of the iron relatively to the age of the culture: the second one, on the iron uptake by the cells, relatively to different concentrations of the chelator EDTA.

Foi desenvolvido um método para análise do ferro acumulado em células de fitoplâncton, usando ferrocianeto de potássio como agente complexante. Com esse objetivo montaram-se duas experiências: uma em que se mostra o acúmulo de ferro em relação à idade das células e uma segunda experiência onde a assimilação é plotada em função da concentração de EDTA no meio de cultura.

Analysis method of intracellular iron accumulated in phytoplankton

Jorge Falcão Paredes^* * Instituto Oceanográfico's visiting professor from Instituto de Investigação Científica de Angola, financed by O.E.A. Present address: Centro de Pesquisas e Desenvolvimento. Salvador, BA Brasil. ; Gilda Schmidt

Instituto Oceanográfico da Universidade de São Paulo

SYNOPSIS

A colorimetric method for the assessment of iron accumulated in the cells of phytoplankton utilizing potassium ferrocyanide was developed. Two experiences with Phaeodactylum tricornutum and Chlorella sp. were carried out: the first one, on uptake of the iron relatively to the age of the culture: the second one, on the iron uptake by the cells, relatively to different concentrations of the chelator EDTA.

RESUMO

Foi desenvolvido um método para análise do ferro acumulado em células de fitoplâncton, usando ferrocianeto de potássio como agente complexante. Com esse objetivo montaram-se duas experiências: uma em que se mostra o acúmulo de ferro em relação à idade das células e uma segunda experiência onde a assimilação é plotada em função da concentração de EDTA no meio de cultura.

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(Recebido em 27/maio/1975)

DAVIES, A. G. 1970. Iron, chelation and the growth of marine phytoplankton. J. mar. biol. Ass. U. K., 50:65-86.
DOIG III, M. T. & MARTIN, D. F. 1971. Effect of humic acids on iron analysis in natural waters. Wat. Res., 5:689-692.
GOLDBERG, E. D. 1952. Iron assimilation by marine diatoms. Biol. Bull. mar. biol. Lab., Woods Hole, 102:243-248.
GROSS, F. & ZEUTHEN, E. 1948. The buoyancy of plankton diatoms: a problem of cell physiology. Proc. R. Soc., ser. B, 135:382-389.
HAYWARD, J. 1968. Studies on the growth of Phaeodactylum tricornutum. J. mar. biol. Ass. U. K., 48:295-302.
NIELSEN, E. S. & WIUM-ANDERSEN, S. 1970. Copper ions as poison in the sea and fresh water. Mar. Biol., 6(2):93-97.
PAREDES, J. F. 1968a Studies on cultures of marine phytoplankton. I. Diatom Ditylum brightwellii West. Mems Inst. Invest. cient. Moçamb., ser. A., 9:156-183.
______ 1968b Studies on cultures of marine phytoplankton. III. Chlorophycean Micromonas squamamata. Mems Inst. Invest. cient. Moçamb., sér. A., 9:249-292.
______ 1968c Studies on cultures of marine phytoplankton. II. Exuviella baltica bloom with reference to a red tide occurred in the coast of Angola. Mems Inst. Invest. cient. Moçamb., ser. A., 9:184-247.
RILEY, J. P. 1965. Analytical chemistry of sea water. In: Riley, J. P. & Skirrow, G., ed. - Chemical oceanography. London, Academic Press, vol.2, p.378.
RYTHER, J. H. & KRAMER, D. D. 1961. Relative iron requirements of some coastal and offshore plankton algae. Ecology, 43:444-446.
WIESSNER, W. 1962. Inorganic micronutrients. In: Lewin, R. A., ed. - Physiology and biochemistry of algae. New York, Academic Press, 929p.

*

Instituto Oceanográfico's visiting professor from Instituto de Investigação Científica de Angola, financed by O.E.A.

Present address: Centro de Pesquisas e Desenvolvimento. Salvador, BA Brasil.

Publication Dates

Publication in this collection
11 June 2012
Date of issue
1977

History

Received
27 May 1975

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] DAVIES, A. G. 1970. Iron, chelation and the growth of marine phytoplankton. J. mar. biol. Ass. U. K., 50:65-86.

[2] DOIG III, M. T. & MARTIN, D. F. 1971. Effect of humic acids on iron analysis in natural waters. Wat. Res., 5:689-692.

[3] GOLDBERG, E. D. 1952. Iron assimilation by marine diatoms. Biol. Bull. mar. biol. Lab., Woods Hole, 102:243-248.

[4] GROSS, F. & ZEUTHEN, E. 1948. The buoyancy of plankton diatoms: a problem of cell physiology. Proc. R. Soc., ser. B, 135:382-389.

[5] HAYWARD, J. 1968. Studies on the growth of Phaeodactylum tricornutum. J. mar. biol. Ass. U. K., 48:295-302.

[6] NIELSEN, E. S. & WIUM-ANDERSEN, S. 1970. Copper ions as poison in the sea and fresh water. Mar. Biol., 6(2):93-97.

[7] PAREDES, J. F. 1968a Studies on cultures of marine phytoplankton. I. Diatom Ditylum brightwellii West. Mems Inst. Invest. cient. Moçamb., ser. A., 9:156-183.

[8] ______ 1968b Studies on cultures of marine phytoplankton. III. Chlorophycean Micromonas squamamata. Mems Inst. Invest. cient. Moçamb., sér. A., 9:249-292.

[9] ______ 1968c Studies on cultures of marine phytoplankton. II. Exuviella baltica bloom with reference to a red tide occurred in the coast of Angola. Mems Inst. Invest. cient. Moçamb., ser. A., 9:184-247.

[10] RILEY, J. P. 1965. Analytical chemistry of sea water. In: Riley, J. P. & Skirrow, G., ed. - Chemical oceanography. London, Academic Press, vol.2, p.378.

[11] RYTHER, J. H. & KRAMER, D. D. 1961. Relative iron requirements of some coastal and offshore plankton algae. Ecology, 43:444-446.

[12] WIESSNER, W. 1962. Inorganic micronutrients. In: Lewin, R. A., ed. - Physiology and biochemistry of algae. New York, Academic Press, 929p.