Efecto de la ablacion peduncular en la maduracion gonadal de Pleoticus muelleri bate (decapoda, solenoceridae). I. interaccion entre muda y desarrollo ovarico

Díaz, Ana Cristina; Petriella, Ana Maria; Fenucce, Jorge Lino

doi:10.1590/S1413-77391997000100006

Resúmenes

The objective of this study was to compare the ovary development of ablated and non-ablated females of Pleoticus muelleri and to evaluate the influence of eyestalk ablation on molting frequency. The females (7-22 g weight) were maintained under culture conditions with 12: 12 fotoperiod, 15-17ÂºC temperature and 31%0 salinity. They were fed on fresh squid, prawn and clam. The ovaries and digestive glands were weighed and fixed for histological examination at 4,9, 15,20, 40, and 50 days. Gonad index varied between 0.925 and 3.747 (control) and 1.457 and 5.035 (ablated). The digestive gland index ranged from 2.059 to 4.520 (control) and 3.471 to 4.471 (ablated). The intermolt duration was 21.7 :j: 3.9 and 23.2 :j: 2.4 for the control and ablated respectively. At the end of the experiment the ovaries of ablated females were mature and those of the control were in primary vitellogenesis. It was concluded that the eyestalk ablation in P. muelleri resulted in precocious maturation of the ovary without changes in the molting frequency. The ovary index was size-independent. Ablation had no effect on the relative weight of digestive gland.

Ovarian maturation; Molt; Culture; Crustacea; Penaeoidea

Este trabalho teve como objetivo comparar o grau de desenvolvimento ovariano de remeas ablacionadas e intactas de Pleoticus muelleri e avaliar a influência da ablação sobre a freqüência da muda. As femeas, de 7 a 22 g de peso, foram mantidas em condições de cultivo com fotoperíodo de 12:12, temperatura entre 15 e 17ÂºC, salinidade de 31 e alimentadas com lula, camarão e bivalvos frescos. Foram pesados e fixados os ovários e hepatopâncreas, realizando um controle histológico nos 4; 9; 15; 20; 40 e 50 dias. O índice gonadossomático variou entre 0,925 e 3,747 (controle) e entre 1,457 e 5,035 (ablacionados). Determinou-se valores de índice hepatopancreático entre 2,059 a 4,520 (controle) e de 3,471 a 4,471 (ablacionados). A duração média da intermuda foi 21,7 :j: 3,9 e 23,2 :j: 2,4 dias para o grupo controle e ablacionados, respectivamente. Ao finalizar o experimento a análise histológica demostrou que os ovários das femeas ablacionadas encontravam-se totalmente amadurecidos e os do controle em vitelogênese primária. Conclui-se que a ablação peduncular acelera a maturação ovariana de P. muelleri, sem modificar a freqüência da muda. O índice ovariano é independente do tamanho no intervalo de peso estudado e a ablação não apresentou efeito sobre o peso relativo dos hepatopâncreas.

Maturação ovariana; Muda; Cultivo; Crustacea; Penaeoidea

Ovarian maturation; Molt; Culture; Crustacea; Penaeoidea

Maturação ovariana; Muda; Cultivo; Crustacea; Penaeoidea

RESEARCH ARTICLES

Efecto de la ablacion peduncular en la maduracion gonadal de Pleoticus muelleri bate (decapoda, solenoceridae). I. interaccion entre muda y desarrollo ovarico

Ana Cristina Díaz^I; Ana Maria Petriella^II; Jorge Lino Fenucce^II

^ICIC (Provincia de Buenos Aires); Departamento de Ciencias Marinas (UNMP)

^IICONICET Departamento de Ciencias Marinas, Universidad Nacional Mar deI Plata (UNMP) (punes 3350, 7600 Mar deI Plata, Argentina)

ABSTRACT

The objective of this study was to compare the ovary development of ablated and non-ablated females of Pleoticus muelleri and to evaluate the influence of eyestalk ablation on molting frequency. The females (7-22 g weight) were maintained under culture conditions with 12: 12 fotoperiod, 15-17ÂºC temperature and 31%0 salinity. They were fed on fresh squid, prawn and clam. The ovaries and digestive glands were weighed and fixed for histological examination at 4,9, 15,20, 40, and 50 days. Gonad index varied between 0.925 and 3.747 (control) and 1.457 and 5.035 (ablated). The digestive gland index ranged from 2.059 to 4.520 (control) and 3.471 to 4.471 (ablated). The intermolt duration was 21.7 :j: 3.9 and 23.2 :j: 2.4 for the control and ablated respectively. At the end of the experiment the ovaries of ablated females were mature and those of the control were in primary vitellogenesis. It was concluded that the eyestalk ablation in P. muelleri resulted in precocious maturation of the ovary without changes in the molting frequency. The ovary index was size-independent. Ablation had no effect on the relative weight of digestive gland.

Descriptors: Ovarian maturation, Molt, Culture, Crustacea, Penaeoidea.

RESUMO

Este trabalho teve como objetivo comparar o grau de desenvolvimento ovariano de remeas ablacionadas e intactas de Pleoticus muelleri e avaliar a influência da ablação sobre a freqüência da muda. As femeas, de 7 a 22 g de peso, foram mantidas em condições de cultivo com fotoperíodo de 12:12, temperatura entre 15 e 17ÂºC, salinidade de 31 e alimentadas com lula, camarão e bivalvos frescos. Foram pesados e fixados os ovários e hepatopâncreas, realizando um controle histológico nos 4; 9; 15; 20; 40 e 50 dias. O índice gonadossomático variou entre 0,925 e 3,747 (controle) e entre 1,457 e 5,035 (ablacionados). Determinou-se valores de índice hepatopancreático entre 2,059 a 4,520 (controle) e de 3,471 a 4,471 (ablacionados). A duração média da intermuda foi 21,7 :j: 3,9 e 23,2 :j: 2,4 dias para o grupo controle e ablacionados, respectivamente. Ao finalizar o experimento a análise histológica demostrou que os ovários das femeas ablacionadas encontravam-se totalmente amadurecidos e os do controle em vitelogênese primária. Conclui-se que a ablação peduncular acelera a maturação ovariana de P. muelleri, sem modificar a freqüência da muda. O índice ovariano é independente do tamanho no intervalo de peso estudado e a ablação não apresentou efeito sobre o peso relativo dos hepatopâncreas.

Descritores: Maturação ovariana, Muda, Cultivo, Crustacea, Penaeoidea.

Full text available only in PDF format.

Texto completo disponível apenas em PDF.

Agradecimientos

Este trabajo ha sido subsidiado por el Programa CABBIO (Centro Argentino Brasilero de Biotecnologia), BID-CONICET 11 Y la Universidad Nacional de Mar dei Plata, Argentina.

(Manuscrito recebido 15 abril 1996; revisto 21 outubro 1996; aceito 26 agosto 1997)

Adiyodi, R G. 1985. Reproduction and its control. In: Bliss, D. E. & Mantel, L. H. eds The Biology of crustacea. New York, Academic Press. 9:147-215.
Aiken, D. E. & Waddy, S. L. 1976. Controlling growth and reproduction in the American lobster. Proc. World Maricult. Soc., 7: 415-430.
Bell, T. A. & Lightner, D.V. 1988. A handbook of normal penaeid shrimp histology. Baton Rouge, World Aq. Soc. 114p.
Boschi, E. E. 1986. La pesqueria deI langostino dei litoral patagónico. Cuadernos de Redes, 20: 1- 8.
Browdy, C. L. 1992. A review of the reproductive biology of Penaeus species: perspectives on controlled shrimp maturation systems for high quality nauplii production. Proc. Special Session on Shrimp Farming, World Aq. Soc. p.22-51.
Browdy, C. L.; Hadani, A, Samocha, T. M. & Loya, Y. 1986. The reproductive performance of wild and pond-reared Penaeus semisu/latus de Haan. Aquaculture, 59: 251-258.
Chamberlain, G. W.; Haby, M. G. & Russell, J. M. 1985. Texas shrimp farming manual: an update on current technology. Texas Agricultural Extension Service. 158p.
Chamberlain, G. W. & Lawrence, A L. 1981. Effect oí light intensity and male and female eyestalk ablation on reproduction of Penaeus stylirostris and Penaeus vannamei. J. Wld Maricult. Soc., 12(2):357-372.
Choy, S. C. 1987. Growth and reproduction oí eyestalk ablated Penaeus canaliculatus (Olivier, 1811) (Crustacea: Penaeidae). J. expl mar. Biol. Ecol., 112: 93-107.
Emmerson, W. D. 1980. Induced maturation of prawn Penaeus indicus. Mar. Ecol. Prog. Ser., 2: 121- 131.
Fyffe, W. E. & O'Connor, J. D. 1974. Characterization and quantification of a crustacean lipovitellin. Comp. Biochem. Physiol., 47B:851-867.
Gendrop-Funes, V. & Valenzuela-Espinoza, E. 1995. AblaciÓD unilateral de Penaeus stylirostris (Stimpson). Ciencias Marinas, 21(4):401-413.
Gibson, R 1982. Feeding and digestion in decapod crustaceans. In: Pruder, G. D.; Langdon, C. J. & Conklin, D. E. eds Proc. 2nd Int. Conf. Aq. Nutrition. p. 59-70.
Haefuer, P. A. & Spaargaren, D. H. 1993. Interactions of ovary and hepatopancreas during the reproductive cycle of Crangon crangon (L.) I. Weight and volume relationship. J. Crust. Biol., 13(3): 523-531.
Hillier, A. G. 1984. Artificial conditions influencing the maturation and spawning of sub-adult Penaeus monodon (Fabricius). Aquaculture, 36: 179-184.
Jeckel, W. H.; Aizp6n de Moreno, J. E. & Moreno, V. J. 1989. Biochemical composition, lipid classes and fatty acids in the ovary of the shrimp Pleoticus muelleri Bate. Comp. Biochem. Physiol., 92B(2): 271-276.
Kurata, H. 1962. Studies on the age and growth of crustaces. Buli. Hokkaido reg. Fish. Res. Lab., 24: 1-115.
Lawrence, A L.; Ward, D.; Missler, S.; Brown, A; McVey, J. & Middleditch, B. S. 1979. Organ indices and biochemicallevels of ova from penaeid shrimp maintained in captivity versus those captured in the wild. Proc. World Maricult. Soc., 10:453-463.
Paulus, J. E. & Laufer, H. 1982. Vitellogenesis in the hepatopancreas and ovaries of Carcinus maenas. Biol. Bull., 163: 375-376.
Pillay, K. K. & Nair, N. B. 1971. The annual reproductive cycles of Uca annulipes, Portunus pelagicus, and Metapenaeus affinis (Decapoda: Crustacea) from the southwest coast of India. Mar. Biol., 11: 152-166.
Quackenbush, L. S. 1989. Yolk protein production in the marine shrimp Penaeus vannamei. J. Crust. Biol.,9(4):509-516.
Quackenbush, L. S. 1991. Regulation of vitellogenesis in penaeid shrimp. In: DeLoach, P. F.; Dougherty, W. J. & Davidson, M. A. eds Frontiers of shrimp research. Amsterdan, Elsevier Science Publishers. p.125-140.
Sokal, R & Rohlf, 1. 1979. Biometría. Madrid, H. Blume. 832p.
Tan Fermin, J. D. 1991. Effects of unilateral eyestalk ablation on ovarian histology and oocyte size frequency of wild and pond-reared Penaeus monodon (Fabricius) broodstock. Aquaculture, 93:77-86.
Tom, M.; Goren, M. & Ovadia, M. 1987. Localization of the vitellin and its possible precursors in various organs of Parapenaeus longirostris. Int. J. Invert. Reprod. Develop., 12:1-12.
Yano, I. & Chinzei, Y. 1987. Ovary is the site of vitellogenin synthesis in kuruma prawn, Penaeus japonicus. Comp. Biochem. Physiol., 86B(2):213- 218.

Fechas de Publicación

Publicación en esta colección
22 Abr 2013
Fecha del número
1997

Histórico

Revisado
21 Oct 1996
Recibido
15 Abr 1996
Acepto
26 Ago 1997

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Adiyodi, R G. 1985. Reproduction and its control. In: Bliss, D. E. & Mantel, L. H. eds The Biology of crustacea. New York, Academic Press. 9:147-215.

[2] Aiken, D. E. & Waddy, S. L. 1976. Controlling growth and reproduction in the American lobster. Proc. World Maricult. Soc., 7: 415-430.

[3] Bell, T. A. & Lightner, D.V. 1988. A handbook of normal penaeid shrimp histology. Baton Rouge, World Aq. Soc. 114p.

[4] Boschi, E. E. 1986. La pesqueria deI langostino dei litoral patagónico. Cuadernos de Redes, 20: 1- 8.

[5] Browdy, C. L. 1992. A review of the reproductive biology of Penaeus species: perspectives on controlled shrimp maturation systems for high quality nauplii production. Proc. Special Session on Shrimp Farming, World Aq. Soc. p.22-51.

[6] Browdy, C. L.; Hadani, A, Samocha, T. M. & Loya, Y. 1986. The reproductive performance of wild and pond-reared Penaeus semisu/latus de Haan. Aquaculture, 59: 251-258.

[7] Chamberlain, G. W.; Haby, M. G. & Russell, J. M. 1985. Texas shrimp farming manual: an update on current technology. Texas Agricultural Extension Service. 158p.

[8] Chamberlain, G. W. & Lawrence, A L. 1981. Effect oí light intensity and male and female eyestalk ablation on reproduction of Penaeus stylirostris and Penaeus vannamei. J. Wld Maricult. Soc., 12(2):357-372.

[9] Choy, S. C. 1987. Growth and reproduction oí eyestalk ablated Penaeus canaliculatus (Olivier, 1811) (Crustacea: Penaeidae). J. expl mar. Biol. Ecol., 112: 93-107.

[10] Emmerson, W. D. 1980. Induced maturation of prawn Penaeus indicus. Mar. Ecol. Prog. Ser., 2: 121- 131.

[11] Fyffe, W. E. & O'Connor, J. D. 1974. Characterization and quantification of a crustacean lipovitellin. Comp. Biochem. Physiol., 47B:851-867.

[12] Gendrop-Funes, V. & Valenzuela-Espinoza, E. 1995. AblaciÓD unilateral de Penaeus stylirostris (Stimpson). Ciencias Marinas, 21(4):401-413.

[13] Gibson, R 1982. Feeding and digestion in decapod crustaceans. In: Pruder, G. D.; Langdon, C. J. & Conklin, D. E. eds Proc. 2nd Int. Conf. Aq. Nutrition. p. 59-70.

[14] Haefuer, P. A. & Spaargaren, D. H. 1993. Interactions of ovary and hepatopancreas during the reproductive cycle of Crangon crangon (L.) I. Weight and volume relationship. J. Crust. Biol., 13(3): 523-531.

[15] Hillier, A. G. 1984. Artificial conditions influencing the maturation and spawning of sub-adult Penaeus monodon (Fabricius). Aquaculture, 36: 179-184.

[16] Jeckel, W. H.; Aizp6n de Moreno, J. E. & Moreno, V. J. 1989. Biochemical composition, lipid classes and fatty acids in the ovary of the shrimp Pleoticus muelleri Bate. Comp. Biochem. Physiol., 92B(2): 271-276.

[17] Kurata, H. 1962. Studies on the age and growth of crustaces. Buli. Hokkaido reg. Fish. Res. Lab., 24: 1-115.

[18] Lawrence, A L.; Ward, D.; Missler, S.; Brown, A; McVey, J. & Middleditch, B. S. 1979. Organ indices and biochemicallevels of ova from penaeid shrimp maintained in captivity versus those captured in the wild. Proc. World Maricult. Soc., 10:453-463.

[19] Paulus, J. E. & Laufer, H. 1982. Vitellogenesis in the hepatopancreas and ovaries of Carcinus maenas. Biol. Bull., 163: 375-376.

[20] Pillay, K. K. & Nair, N. B. 1971. The annual reproductive cycles of Uca annulipes, Portunus pelagicus, and Metapenaeus affinis (Decapoda: Crustacea) from the southwest coast of India. Mar. Biol., 11: 152-166.

[21] Quackenbush, L. S. 1989. Yolk protein production in the marine shrimp Penaeus vannamei. J. Crust. Biol.,9(4):509-516.

[22] Quackenbush, L. S. 1991. Regulation of vitellogenesis in penaeid shrimp. In: DeLoach, P. F.; Dougherty, W. J. & Davidson, M. A. eds Frontiers of shrimp research. Amsterdan, Elsevier Science Publishers. p.125-140.

[23] Sokal, R & Rohlf, 1. 1979. Biometría. Madrid, H. Blume. 832p.

[24] Tan Fermin, J. D. 1991. Effects of unilateral eyestalk ablation on ovarian histology and oocyte size frequency of wild and pond-reared Penaeus monodon (Fabricius) broodstock. Aquaculture, 93:77-86.

[25] Tom, M.; Goren, M. & Ovadia, M. 1987. Localization of the vitellin and its possible precursors in various organs of Parapenaeus longirostris. Int. J. Invert. Reprod. Develop., 12:1-12.

[26] Yano, I. & Chinzei, Y. 1987. Ovary is the site of vitellogenin synthesis in kuruma prawn, Penaeus japonicus. Comp. Biochem. Physiol., 86B(2):213- 218.