Sensitivity of the marine benthic copepod Tisbe biminiensis (copepoda, harpacticoida) to potassium dichromate and sediment particle size

Araújo-Castro, Cristiane M. V.; Souza-Santos, Lília P.; Torreiro, Anny Gabrielle A .G.; Garcia, Karina S.

Abstracts

For the future use of the marine benthic copepod Tisbe biminiensis in solid-phase sediment toxicological bioassays, the present study investigated the effect of muddy sediment from the Maracaípe estuary (northeastern Brazil), sediment particle size and the reference toxicant potassium dichromate on the species. Muddy sediment from Maracaípe can be used as control sediment, since it does not interfere in the copepod life-cycle and has metal contamination levels that are unlikely to produce any detrimental biological effects on benthic invertebrates. Neither survival nor fecundity was affected by grain size, suggesting that this species can be used with any kind of sediment from muddy to sandy. The sensitivity of T. biminiensis to K2Cr2O7 in acute tests was similar to that of other organisms. The LC50 (lethal concentration to 50% of the test organisms) medium values for T. biminiensis were 7.51, 4.68 and 3.19 mg L-1 for Cr in 48, 72 and 96 h, respectively. These results suggest that T. biminiensis is a promising organism for use in solid-phase sediment toxicity assessments.

Toxicity; Meiofauna; Toxicity test; Potassium dichromate; Sediment grain size

Visando o uso futuro do copépodo marinho bentônico Tisbe biminiensis em bioensaios toxicológicos de sedimentos na fase sólida, o presente estudo investigou o efeito do sedimento lamoso do estuário de Maracaípe (Nordeste do Brasil). Foram considerados a granolometria e o tóxico de referência dicromato de potássio sobre a espécie. O sedimento lamoso de Maracaípe pode ser usado como controle, uma vez que não interfere no ciclo de vida do copépodo e possui níveis de contaminação de metais que não causariam efeitos biológicos em invertebrados bentônicos. Nem a sobrevivência ou fecundidade foi afetada pelo tamanho do grão, sugerindo que esta espécie pode ser usada com qualquer tipo de sedimento, de lama a areia. A sensibilidade de T. biminiensis ao K2Cr2O7 em testes agudos foi similar a de outros organismos. Os valores de CL50 (concentração letal a 50% dos organismos) para T. biminiensis foram em média 7,51, 4,68 e 3,19 mg L-1 para o Cr em 48, 72 e 96h, respectivamente. Estes resultados sugerem que T. biminiensis é um organismo promissor para uso em avaliações de toxicidade com a fase sólida do sedimento.

Toxicidade; Meiofauna; Testes de toxicidade; Dicromaro de potássio; Granulometria

ABESSA, D. M. S.; SOUZA, E. C. P. M. Sensitivity of the amphipod Tiburonella viscana (Platyischnopidae) to K₂Cr₂O₇ Braz. Arch. Biol. Technol, v. 46, p. 53-55, 2003.
ALLEN, H. E.; EU, G.; DENG, B. Analysis Acid-volatile Sulfid (AVS) and simultaneously extracted metals (SEM) for the estimation of potential toxicity in aquatic sediments. Environ. Toxicol. Chem., v. 12, p. 1441 - 1453, 1993.
ARAÚJO-CASTRO, C. M. V.; SOUZA-SANTOS, L. P. Are the diatoms Navícula sp. and Thalassiosita fluviatilis suitable to be fed to the benthic harpacticoid copepod Tisbe biminiensis? J expl mar. Biol. Ecol., v. 327, p. 58-69, 2005.
COULL, B. C.; CHANDLER, G. T. Pollution and meiofauna: Field, laboratory, and mesocosm studies. Oceanogr. mar. Biol. a. Rev., v. 30, p. 191-271, 1992.
DeWITT, T. H.; REDMOND, M. S.; SEWALL, J.E.; SWARTZ, R.C. Development of a chronic sediment toxicity test for marine benthic amphipods. Contribution USEPA/ERLNarragansett, No. N- 240, 1992.
ENVIRONMENT CANADA. Biological test method: acute test for sediment toxicity using marine or estuarine amphipods. Environment Canada, Ottawa, 1992. Report EPS 1/RM/26
GUILLARD, R. R. L. Culture of phytoplankton for feeding marine invertebrates. In: SMITH, W. L.; CHANLEY, M. H. (Eds). Culture of marine invertebrates animals. Plenum Press. N. Y., pp. 26-60. 1975.
HAMILTON, M. A., RUSSO, R. C., THURSTON, R. V. Trimmed Spearman-Karber Method for estimating median lethal concentrations in toxicity bioassay. Envir. Sci. Technol. v. 11, p. 714-719, 1977.
INGERSOLL, C.G. Sediment tests. In: RAND, G. M. (ED.). Fundamentals of aquatic toxicology: effects, environmental fate, and risk assessment . Washington: Taylor and Francis, 1995. p. 231-255.
ISO 14669. Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea) Geneva, Switzerland. First edition. 1999-04-01, 1999.
KENNISH, M.J. Pollution impacts on marine biotic communities Marine Science Series. CRC Press., 1997, 308p.
LARRAIN, A.; SOTO, E.; SILVA, J.; BAY-SCHMITH, E. Sensitivity of the meiofaunal copepod Tisbe longicornis to K₂Cr₂O₇ under varying temperature regimes. Bull. environ. Contamin. Toxicol, v. 61, p. 391-396, 1998.
LOTUFO, G. R.; ABESSA, D.M. S. Testes de toxicidade com sedimento total e água intersticial estuarinos utilizando copépodos bentônicos. In: NASCIMENTO, I. A.; SOUSA, E. C. P. M.; NIPPER, M. Métodos em ecotoxicologia marinha: aplicações para o Brasil. São Paulo, Artes Gráficas e Industriais, 2002. p. 151-162.
LORENZON, S.; FRANCESE, M.; SMITH, V.J.; FERRERO, E. A. Heavy metals affect the circulating haemocyte number in the shrimp Palaemon elegans Fish Shellfish Immunol., , v. 11, p. 459-472, 2001.
McCREADY, S.; BIRCH, G.F.; LONG, E.R. Metallic and organic contaminants in sediments of Sydney Harbour, Australia and vicinity - A chemical dataset for evaluating sediment quality guidelines. Environ. Int, v. 32, p. 455-465, 2006.
MELO, S.L.R.; NIPPER, M. Sediment toxicity tests using the burrowing amphipod Tiburonella viscana (Amphipoda: Platyischnopidae). Ecotoxicol. Environ. Saf., v. 66, p. 412-420, 2007.
NIPPER, M. Current approaches and future directions for contaminant-related impact assessments in coastal environments: Brazilian perspective. Aquat. Ecosys. Health Mgmt, v. 3, p. 433-447, 2000.
PINTO, C. S. C.; SOUZA-SANTOS, L. P.; SANTOS, P. J. P. Development and population dynamics of Tisbe biminiensis (Copepoda: Harpacticoida) reared on different diets. Aquaculture, v. 198, p. 253-267, 2001.
POUNDS, N. A.; HUTCHINSON, T. H.; WILLIAMS, T. D.; WHITING, P.; DINAN, L. Assessment of putative endocrine disrupters in an in vivo crustacean assay and an in vitro insect assay. Mar. environ. Res, v. 54, p. 709-713, 2002.
SILVA, E. M.; CHASTINET, C. B. A.; NAVARRO, M. F. T.; BARROS, A. F.; CAVALCANTE, P.; MOTA, M. F. V. Utilização de ensaios de ecotoxicidade com Tisbe holothuriae (Copepoda: Harpacticoida) em programas de avaliação da toxicidade de efluentes complexos. In: ESPÍNDOLA, E. L .G.; PASCHOAL, C. M. R. B.; ROCHA, O.; BOHRER, M. B. C.; OLIVEIRA NETO, A.L. Ecotoxicologia: perspectivas para o século XXI. São Carlos: RIMA, 2000. p. 451-460.
SILVÉRIO, P.F. Partição, biodisponibilidade e toxidade de metais pesados a organismos bentônicos em sedimentos São Carlos,. 78 f. Dissertação (Mestrado em Química) - Instituto de Química, Universidade Federal de São Carlos, São Carlos, SP, 1999.
SOUZA-SANTOS, L.P.; PASTOR, J.M.O.; FERREIRA, N.G.; COSTA, W.M.; ARAÚJO-CASTRO, C.M.V.; SANTOS, P.J.P. Developing the harpacticoid copepod Tisbe biminiensis culture: testing for salinity tolerance, ration levels, presence of sediment and density dependent analyses. Aquat. Res, v. 37, p. 1516-1523, 2006.
THOMAS, K. V.; BARNARD, N.; COLLINS, K.; EGGLETON, J. Toxicity characterization of sediment porewaters collected from UK estuaries using a Tisbe battagliai bioassay. Chemosphere, v. 53, p. 1105-1111, 2003.
U.S. EPA. Short term methods for estimating the chronic toxicity and effluents and receiving waters to marine and estuarine organisms. EPA/600/4-87/028, 1988.
VOLKMANN, B. Tisbe (Copepoda, Harpacticoida) species from Bermuda and zoogeographical considerations. Arch. Oceanogr. Limnol, v. 19,p. 1-76, 1979.

Publication Dates

Publication in this collection
22 Apr 2009
Date of issue
Mar 2009

History

Accepted
15 Aug 2008
Received
24 Jan 2008
Reviewed
03 June 2008

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] ABESSA, D. M. S.; SOUZA, E. C. P. M. Sensitivity of the amphipod Tiburonella viscana (Platyischnopidae) to K₂Cr₂O₇ Braz. Arch. Biol. Technol, v. 46, p. 53-55, 2003.

[2] ALLEN, H. E.; EU, G.; DENG, B. Analysis Acid-volatile Sulfid (AVS) and simultaneously extracted metals (SEM) for the estimation of potential toxicity in aquatic sediments. Environ. Toxicol. Chem., v. 12, p. 1441 - 1453, 1993.

[3] ARAÚJO-CASTRO, C. M. V.; SOUZA-SANTOS, L. P. Are the diatoms Navícula sp. and Thalassiosita fluviatilis suitable to be fed to the benthic harpacticoid copepod Tisbe biminiensis? J expl mar. Biol. Ecol., v. 327, p. 58-69, 2005.

[4] COULL, B. C.; CHANDLER, G. T. Pollution and meiofauna: Field, laboratory, and mesocosm studies. Oceanogr. mar. Biol. a. Rev., v. 30, p. 191-271, 1992.

[5] DeWITT, T. H.; REDMOND, M. S.; SEWALL, J.E.; SWARTZ, R.C. Development of a chronic sediment toxicity test for marine benthic amphipods. Contribution USEPA/ERLNarragansett, No. N- 240, 1992.

[6] ENVIRONMENT CANADA. Biological test method: acute test for sediment toxicity using marine or estuarine amphipods. Environment Canada, Ottawa, 1992. Report EPS 1/RM/26

[7] GUILLARD, R. R. L. Culture of phytoplankton for feeding marine invertebrates. In: SMITH, W. L.; CHANLEY, M. H. (Eds). Culture of marine invertebrates animals. Plenum Press. N. Y., pp. 26-60. 1975.

[8] HAMILTON, M. A., RUSSO, R. C., THURSTON, R. V. Trimmed Spearman-Karber Method for estimating median lethal concentrations in toxicity bioassay. Envir. Sci. Technol. v. 11, p. 714-719, 1977.

[9] INGERSOLL, C.G. Sediment tests. In: RAND, G. M. (ED.). Fundamentals of aquatic toxicology: effects, environmental fate, and risk assessment . Washington: Taylor and Francis, 1995. p. 231-255.

[10] ISO 14669. Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea) Geneva, Switzerland. First edition. 1999-04-01, 1999.

[11] KENNISH, M.J. Pollution impacts on marine biotic communities Marine Science Series. CRC Press., 1997, 308p.

[12] LARRAIN, A.; SOTO, E.; SILVA, J.; BAY-SCHMITH, E. Sensitivity of the meiofaunal copepod Tisbe longicornis to K₂Cr₂O₇ under varying temperature regimes. Bull. environ. Contamin. Toxicol, v. 61, p. 391-396, 1998.

[13] LOTUFO, G. R.; ABESSA, D.M. S. Testes de toxicidade com sedimento total e água intersticial estuarinos utilizando copépodos bentônicos. In: NASCIMENTO, I. A.; SOUSA, E. C. P. M.; NIPPER, M. Métodos em ecotoxicologia marinha: aplicações para o Brasil. São Paulo, Artes Gráficas e Industriais, 2002. p. 151-162.

[14] LORENZON, S.; FRANCESE, M.; SMITH, V.J.; FERRERO, E. A. Heavy metals affect the circulating haemocyte number in the shrimp Palaemon elegans Fish Shellfish Immunol., , v. 11, p. 459-472, 2001.

[15] McCREADY, S.; BIRCH, G.F.; LONG, E.R. Metallic and organic contaminants in sediments of Sydney Harbour, Australia and vicinity - A chemical dataset for evaluating sediment quality guidelines. Environ. Int, v. 32, p. 455-465, 2006.

[16] MELO, S.L.R.; NIPPER, M. Sediment toxicity tests using the burrowing amphipod Tiburonella viscana (Amphipoda: Platyischnopidae). Ecotoxicol. Environ. Saf., v. 66, p. 412-420, 2007.

[17] NIPPER, M. Current approaches and future directions for contaminant-related impact assessments in coastal environments: Brazilian perspective. Aquat. Ecosys. Health Mgmt, v. 3, p. 433-447, 2000.

[18] PINTO, C. S. C.; SOUZA-SANTOS, L. P.; SANTOS, P. J. P. Development and population dynamics of Tisbe biminiensis (Copepoda: Harpacticoida) reared on different diets. Aquaculture, v. 198, p. 253-267, 2001.

[19] POUNDS, N. A.; HUTCHINSON, T. H.; WILLIAMS, T. D.; WHITING, P.; DINAN, L. Assessment of putative endocrine disrupters in an in vivo crustacean assay and an in vitro insect assay. Mar. environ. Res, v. 54, p. 709-713, 2002.

[20] SILVA, E. M.; CHASTINET, C. B. A.; NAVARRO, M. F. T.; BARROS, A. F.; CAVALCANTE, P.; MOTA, M. F. V. Utilização de ensaios de ecotoxicidade com Tisbe holothuriae (Copepoda: Harpacticoida) em programas de avaliação da toxicidade de efluentes complexos. In: ESPÍNDOLA, E. L .G.; PASCHOAL, C. M. R. B.; ROCHA, O.; BOHRER, M. B. C.; OLIVEIRA NETO, A.L. Ecotoxicologia: perspectivas para o século XXI. São Carlos: RIMA, 2000. p. 451-460.

[21] SILVÉRIO, P.F. Partição, biodisponibilidade e toxidade de metais pesados a organismos bentônicos em sedimentos São Carlos,. 78 f. Dissertação (Mestrado em Química) - Instituto de Química, Universidade Federal de São Carlos, São Carlos, SP, 1999.

[22] SOUZA-SANTOS, L.P.; PASTOR, J.M.O.; FERREIRA, N.G.; COSTA, W.M.; ARAÚJO-CASTRO, C.M.V.; SANTOS, P.J.P. Developing the harpacticoid copepod Tisbe biminiensis culture: testing for salinity tolerance, ration levels, presence of sediment and density dependent analyses. Aquat. Res, v. 37, p. 1516-1523, 2006.

[23] THOMAS, K. V.; BARNARD, N.; COLLINS, K.; EGGLETON, J. Toxicity characterization of sediment porewaters collected from UK estuaries using a Tisbe battagliai bioassay. Chemosphere, v. 53, p. 1105-1111, 2003.

[24] U.S. EPA. Short term methods for estimating the chronic toxicity and effluents and receiving waters to marine and estuarine organisms. EPA/600/4-87/028, 1988.

[25] VOLKMANN, B. Tisbe (Copepoda, Harpacticoida) species from Bermuda and zoogeographical considerations. Arch. Oceanogr. Limnol, v. 19,p. 1-76, 1979.