Metazoan parasites of Hoplias aff. malabaricus, Trachelyopterus galeatus and Schizodon borellii (Osteichthyes) from the Protected Area and its main tributary, Brazil

Metazoários parasitos de Hoplias aff. malabaricus, Trachelyopterus galeatus e Schizodon borellii (Osteichthyes) da Área de Proteção Ambiental e seu principal afluente, Brasil

Abstract

A study of the parasite-host interactions was conducted in the protected area popularly known as the “Refúgio Biológico de Santa Helena” and its tributary, the São Francisco Falso River. For each of the three host species, Schizodon borellii, Hoplias aff. malabaricus, and Trachelyopterus galeatus, 30 fish were collected from 2018 to 2019. A total of 2172 parasites were identified among the three host species. Among these, the Monogenea class had the highest number of species, with 26 taxa, followed by Copepoda with eight taxa, Digenea with six taxa, and Cestoda and Nematoda with one taxon each. Eleven new records of infection/infestation were found among the host species such as Urocleidoides paradoxus, Urocleidoides ramentacuminatus, Rhinoxenus arietinus, Mymarothecioides sp. (Monogenea), Ergasilus cf. bryconis, Lernaea devastatrix, and Brasergasilus sp. (Copepoda) parasitizing S. borellii. Trinigyrus sp., Vancleaveus sp. (Monogenea), Gamispinus diabolicus (Copepoda) present in T. galeatus, and Gamidactylus jaraquensis (Copepoda) present in H. aff. malabaricus. This study contributes to the record of parasite species occurrence in the vicinity of a protected area and helps fill gaps in the knowledge of fish parasitic fauna in the Neotropical region.

Keywords:
Helminths; crustaceans; Actinopterygii; Atlantic Forest; São Francisco Falso River

Resumo

Foi realizado um estudo das interações parasitas-hospedeiros na área de proteção popularmente conhecida como “Refúgio Biológico de Santa Helena” e seu afluente, o Rio São Francisco Falso. Para cada uma das três espécies hospedeiras, Schizodon borellii, Hoplias aff. malabaricus e Trachelyopterus galeatus, foram coletados 30 peixes entre 2018 e 2019. Foi identificado um total de 2.172 parasitos entre as três espécies hospedeiras. Dentre estes, a classe Monogenea apresentou o maior número de espécies, com 26 táxons, seguida por Copepoda com oito táxons, Digenea com seis táxons, e Cestoda e Nematoda com um táxon cada. Foram encontrados onze novos registros de infecção/infestação entre as espécies hospedeiras tais como Urocleidoides paradoxus, Urocleidoides ramentacuminatus, Rhinoxenus arietinus, Mymarothecioides sp. (Monogenea), Ergasilus cf. bryconis, Lernaea devastatrix e Brasergasilus sp. (Copepoda) parasitando S. borellii. Trinigyrus sp., Vancleaveus sp. (Monogenea), Gamispinus diabolicus (Copepoda) presentes em T. galeatus, e Gamidactylus jaraquensis (Copepoda) em H. aff. malabaricus. Este estudo contribui para o registro da ocorrência de espécies de parasitos nas proximidades de uma área protegida, e ajuda a preencher lacunas no conhecimento da fauna parasitária de peixes na região Neotropical.

Palavras-chave:
Helmintos; crustáceos; Actinopterygii; Mata Atlântica; Rio São Francisco Falso

Introduction

South America has the highest diversity of freshwater fish in the world (Reis et al., 2016; Birindelli & Sidlauskas, 2018). The orders Characiformes and Siluriformes have the highest species richness, with approximately four thousand valid species (Fricke et al., 2023). Hoplias aff. malabaricus Bloch, 1794, Schizodon borellii Boulenger, 1900 (Characiformes), and Trachelyopterus galeatus Linnaeus, 1766 (Siluriformes) are commonly found in the Paraná–Paraguay River basins, especially after the flooding of the Sete Quedas waterfall (Júlio et al., 2009; Reis et al., 2016; Ota et al., 2018; Reis et al., 2020).

Fish have been on Earth for a long time compared to other vertebrates and have provided more niches for invertebrates to conquer, being responsible for hosting more species of parasites than any other vertebrate group (Thatcher, 2006). Over the years, several authors have attempted to estimate parasite biodiversity and have estimated that one-third of the total species on Earth are parasites (Poulin & Morand, 2004; Luque et al., 2017).

Despite these uncertain estimates, efforts have been made in the form of catalogs on ictioparasitology in the Neotropical Region, including those on Monogenea (Cohen et al., 2013), Digenea (Kohn et al., 2007), Cestoda (Rego, 2000; Justo et al., 2017; Alves et al., 2017), Nematoda (Moravec, 1998; Luque et al., 2011), Acanthocephala (Santos et al., 2008), Isopoda (Thatcher, 2000), Copepoda (Luque et al., 2013), and all parasitological groups such as the study by Eiras et al. (2010). Notably, these host species, such as S. borelli, have previously been studied in other locations (Machado et al., 1996; Lacerda et al., 2007; Karling et al., 2011; Karling et al., 2014), and T. galeatus (Pavanelli & Santos, 1990; Kohn et al., 2011; Yamada et al., 2017; Yamada et al., 2021) and H. aff. malabaricus, which are considered the most studied hosts in various regions of Brazil (Gião et al., 2020; Lima et al., 2022; Diniz et al., 2022; Bueno et al., 2022; Duarte et al., 2023).

With increasing development of human activities, new challenges have emerged as mitigation measures for biodiversity conservation arising from changes in the biotic and abiotic environments (Kueffer & Kaiser-Bunbury, 2014). Areas created as environmental protection, is a way to keep intact this diversity of organisms, and transform unmanaged lands into well-managed entities that maintain this diversity (Rylands & Brandon, 2005). Conducting species surveys plays an important role in maintaining and understanding this biodiversity, assisting in a geographical analysis or changes in the composition of the biota (Wilson, 1988). With increasing research on global biodiversity in freshwater environments, it is becoming increasingly evident that parasites are a fundamental part of this diversity. Thus, they have been the least quantified group of organisms, despite playing fundamental roles as integral components of food webs and ecosystem functions, are significant in quantity and richness, therefore, they should not be neglected (Marcogliese, 2004; Thatcher, 2006; Lafferty et al., 2008; Timi & Poulin, 2020; Williams et al., 2022).

Considering the lack of ictioparasitological studies in the protected area and its main tributary, the São Francisco Falso River, this study aimed to provide the first record of parasite interactions with the hosts H. aff. malabaricus, T. galeatus and S. borellii. In addition to providing parasitological and ecological data on parasite diversity in this new study environment.

Material and Methods

Study area and host collection

The area of protection, referred to in the study region as the “Refúgio Biológico de Santa Helena”, was created in 1984 and has an area of 1,482.05 hectares (142.1 km²) and a perimeter of 30 km (Kliver, 2010). According to the same author, this ecosystem is located entirely in the municipality of Santa Helena, State of Paraná, and is isolated by an excavated canal with a concrete bridge and a gate for controlling the entry and exit of people, thus making it an artificial island.

The São Francisco Falso River was chosen to represent the area of influence of the aforementioned Conservation Unit owing to its important contribution to the formation of the Itaipu Reservoir, which is one of the largest floodplains in the municipality of Santa Helena, Paraná. This river has a watercourse of 127.04 km (Fronza, 2019), an area of 1,554 km², a perimeter of 227.62 km, and covers the municipalities of Céu Azul, Diamante do Oeste, Matelândia, Ramilândia, Santa Helena, Santa Tereza do Oeste, São José das Palmeiras, São Pedro do Iguaçu, and Vera Cruz do Oeste (Lima et al., 2015).

The fish species studied were selected because they had the highest numerical representation within the collections. Thirty individuals of each fish species collected in the project were selected for parasitological studies. Sampling points were distributed around the “Refúgio Biológico de Santa Helena” (RBSH) along the course of the São Francisco Falso River (RSFF), totaling eight (8) sampling points (RBSH1: -24°51'15.12”S-54°21'21.12”W; RBSH2: 24°49'39.97”S-54°21'27.63”W; RBSH3: 24°48'30.50”S-54°21'5.33”W; RSFF1: 24°51'41.90”S-54°17'18.50”W; RSFF2: 24°53'14.14”S-54°13'6.60”W; RSFF3: 24°53'53.84” S-54°13'15.12”W; RSFF4: 24°53'18.56”S-54°13'30.32”W; RSFF5: 24°55'7.38”S-54°12'11.87”W) (Figure 1).

Figure 1
Brazilian map showing the sampling sites in the Refúgio Biológico de Santa Helena (RBSH) and Rio São Francisco Falso (RSFF), State of Paraná. (QGIS Geographic Information System. Open Source Geospatial Foundation Project).

Representative specimens of the fish were deposited in the fish collections of Nupélia: H. aff. malabaricus (NUP:23044), S. borelli (NUP:23037), and T. galeatus (NUP:23107).

Parasitological analysis

The following infection/infestation sites were analyzed: nasal cavities, gill filaments, intestine, eyes, heart, urinary bladder, and musculature. All host necropsy procedures, preservation, and parasite preparation were performed according to Eiras et al. (2006).

Representative specimens were deposited at the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC) and the Helminthological Collection of the Institute of Biosciences at Unesp Botucatu (CHIB).

Data analysis

To test the sufficiency of the samples, the species accumulation curve was calculated using the iNEXT package (Hsieh et al., 2016) was used. The ggplot2 package by (Wickham, 2016) was used to generate the parasite richness graphs. The ecological descriptors (Abundance, Prevalence, Mean abundance and Mean intensity) were calculated according to the method described by Bush et al. (1997). The parasitic diversity index was calculated using the Brillouin calculation (HB), and the Berger–Parker index (d) was used for parasitic dominance. All analyses were performed using the R software (R Core Team, 2020).

Results

In the study, 40 parasite taxa and 2172 specimens were found, divided among the groups (Monogenea, Digenea, Cestoda, Nematoda, and Copepoda), present in the three host species analyzed in this study (see Tables 1, 2 and 3). All host individuals were parasitized by at least one parasite species.

Table 1
Total diversity of parasite species found in the host: Schizodon borellii.

Parasite species	SI	A	P (%)	MA ± SE	MII ± SE	NIR
Monogenea
Jainus piava CHIOC- 39770	G	142	30.0	4.7 ± 16.8	15.7 ± 28.7
Urocleidoides paradoxus CHIOC- 39779 a-d	G	24	16.6	0.8 ± 2.5	4.8 ± 4.8	X
Urocleidoides ramentacuminatus CHIOC-39780 a-d	G	14	30.0	0.4 ± 0.8	1.5 ± 0.7	X
Rhinoxenus arietinus CHIOC- 39772 a-d	NC	32	33.3	1.0 ± 2.6	3.2 ± 3.8	X
Tereancistrum parvus CHIOC- 39774	G	5	13.3	0.1 ± 0.4	1.25 ± 0.5
Tereancistrum paranaenses CHIOC- 39773 a-c	G	3	10.0	0.1 ± 0.3	1.0
Mymarothecioides sp.	G	7	3.3	0.2 ± 0.1	7.0	X
Dactylogyridae gen. sp.1	G	1	3.3	0.03 ± 0.1	1.0
Dactylogyridae gen. sp.3	G	45	30.0	1.5 ± 2.7	5.0 ± 2.8
Digenea
Austrodiplostomun compactum (metacercariae) CHIOC- 39771	E	1	3.3	0.03 ± 0.1	1.0
Copepoda
Gamispatulus schizodontis CHIBB 702L-703L	NC	164	60.0	5.4 ± 8.8	9.1 ± 9.9
Ergasilus cf. bryconis	G	16	20.0	0.5 ± 1.5	2.6 ± 2.7	X
Lernaea devastatrix CHIBB 693L; 694L e 695L	G	6	13.3	0.2 ± 0.5	1.5 ± 0.5	X
Brasergasilus sp.	NC	1	3.3	0.03 ± 0.1	1.0	X

• SI: site of infection; G: gills; NC: nasal cavity; E: eyes; A: abundance; P (%): prevalence; MA: mean abundance; MII: mean intensity of infection; SE: standard error; NIR: new infection/infestation record.

Table 2
Total diversity of parasite species found in the host: Trachelyopterus galeatus.

Monogenea	SI	A	P (%)	MA ± SE	MII ± SE	NIR
Cosmetocleithrum laciniantun CHIOC-39764 a-d	G	622	90.0	20.7 ± 20.2	23 ± 20
Cosmetocleithrum baculum CHIOC-39766 a-d	G	33	53.0	1.1 ± 1.5	2.0 ± 1.5
Cosmetocleithrum galeatum CHIOC- 39767 a-c	G	68	66.6	2.2 ± 2.9	3.5 ± 3.0
Cosmetocleithrum spathulatum CHIOC- 39768 a-c	G	318	93.0	10.6 ± 10.1	11.3 ± 10.1
Vancleaveus sp.	G	2	6.6	0.06 ± 0.2	1.0	X
Trinigyrus sp.	G	1	3.3	0.03 ± 0.1	1.0	X
Digenea
Microrchis oligovitellum CHIOC- 39765 a-b	I	24	50.0	0.8 ± 0.9	1.6 ± 0.6
Cestoda
Cangatiella arandasi CHIOC-39769	I	3	10.0	0.1 ± 0.3	1.0
Copepoda
Gamispinus diabolicus CHIBB 704L-705L -706L	NC	33	40.0	1.1 ± 1.8	2.7 ± 2.0	X

• SI: site of infection; G: gills; NC: nasal cavity; I: intestine; A: abundance; P (%): prevalence; MA: mean abundance; MII: mean intensity of infection; SE: standard error; NIR: new infection/infestation record.

Table 3
Total diversity of parasite species found in the host: Hoplias aff. malabaricus.

Monogenea	SI	A	P (%)	MA ± SE	MII ± SE	NIR
Anacanthorus sp.1	G	2	6.6	0.06 ± 0.2	1.0
Anacanthorus sp.2	G	1	3.3	0.03 ± 0.1	1.0
Anacanthorus sp.3	G	4	3.3	0.13 ± 0.5	2.0 ± 1.4
Urocleidoides brasiliensis CHIOC-39775 a-b	G	8	10.0	0.26 ± 0.9	2.6 ± 2.0
Urocleidoides cuiabai CHIOC-39776 a-b	G	151	73.0	5 ± 5.1	6.8 ± 4.8
Urocleidoides paranae CHIOC- 39778 a-d	G	35	13.0	1.16 ± 4.9	8.7 ± 12.2
Urocleidoides naris CHIOC- 39777 a-f	NC	6	10.0	0.2 ± 0.7	2.0 ± 1.7
Urocleidoides sp.1	G	82	70.0	2.8 ± 3.2	4.0 ± 3.2
Urocleidoides sp.2	G	8	6.6	0.2 ± 1.2	4.0 ± 4.2
Dactylogyridae gen.sp.1	G	15	13.0	0.5 ± 1.4	3.75 ± 1.5
Dactylogyridae gen.sp.2	G	66	50.0	2.2 ± 3.0	4.4 ± 3.0
Digenea
Austrodiplostomum compactum (metacercariae) CHIOC-39783	E	9	20.0	0.9 ± 0.7	1.5 ± 0.8
Clinostomum dimorphum (metacercariae)	H/I	7	20.0	0.2 ± 0.5	1.1 ± 0.4
Clinostomum sp. (metacercariae) CHIOC -39784	I	2	6.6	0.06 ± 0.3	1.0
Phyllodistomum sp. CHIOC- 39786 a-b	UB	8	10.0	0.2 ± 0.9	2.6 ± 2.0
Nematoda
Contracaecum sp. (larvae) CHIOC- 39785	I	10	13.0	0.2 ± 1.2	1.1 ± 3.0
Copepoda
Gamidactylus jaraquensis CHIBB 698L - 699L	NC	43	53.0	1.4 ± 1.8	2.68 ± 1.8	X
Gamispatulus schizodontis CHIBB 700L - 701L	NC	37	33.0	1.2 ± 2.7	3.7 ± 3.7
Lernaea devastatrix CHIBB 696L e 697L	G	96	53.0	3.2 ± 5.1	6.0 ± 5.8
Lernaea devastatrix	NC	7	20.0	0.2 ± 0.6	1.5 ± 0.8

• SI: site of infection; G: gills; H: heart; NC: nasal cavity; I: intestine; UB: urinary bladder; E: eyes; A: abundance; P (%): prevalence; MA: mean abundance; MII: mean intensity of infection; SE: standard error; NIR: new infection/infestation record.

Although the available literature indicates that 30 individuals are adequate for parasitological studies, as shown in Figure 2, the species accumulation curve for S. borellii did not show stability, whereas the other two species reached stability with the amount collected.

Figure 2
Accumulation curve of parasite species by hosts collected.

Among the host species, H. aff. malabaricus had the highest parasite richness, with 19 taxa, followed by S. borellii with 14 taxa, and T. galeatus with nine taxa (Figure 3).

Figure 3
Total richness by parasite groups in each host species.

The results obtained through the Brillouin index (HB) showed that H. aff. malabaricus had the highest diversity, followed by T. galeatus and S. borellii. The Berger-Parker index (d) showed that T. galeatus had the highest species dominance, followed by S. borellii and H. aff. malabaricus (Table 4).

Table 4
Brillouin index (HB) and Berger-Parker index (d), among the hosts species.

Host	Brillouin index (HB)	Berger-Parker index(d)
Schizodon borellii	0.45 ± 0.39	0.74 ± 1.22
Trachelyopterus galeatus	0.80 ± 0.25	2.41 ± 2.13
Hoplias aff. malabaricus	1.05 ± 0.39	0.37 ± 0.31

Discussion

The Class Monogenea was the most expressive of the groups, with high parasite richness and abundance (61.9% of the parasites found), which may be related to some factors, the environment in which their hosts live, such as lakes or power plant reservoirs, which are characterized as lentic; thus, the specify, due their monoxenic life cycle, the free-swimming larvae can find their host more easily (Lizama et al., 2006). In addition, this group is considered to be the richest and most diverse among fish parasites which are highly specific to their hosts (Poulin & Morand, 2004; Kuchta et al., 2020).

Crustaceans were the second most diverse group in this study, accounting for 19% of the samples. Among the groups of metazoan parasites in freshwater fish, crustaceans Branchiura, Copepoda, and Isopoda stand out for their importance and diversity (Tavares-Dias et al., 2015). These three groups represent a large part of the parasitic crustacean fauna in the Neotropical region and deserve attention because they highly impact their hosts which are found mainly in fish farms or natural environments (Pavanelli et al., 2013). Ergasilids represent the fourth largest family of freshwater copepods, with over 60 species in the freshwater region of Brazil (Luque et al., 2013). Among those found in this study, G. schizodontis has a generalist habit, as it occurs in a variety of hosts and has been reported in four families: Anostomidae, Erythrinidae, Pimelodidae and Serrasalmidae (Narciso & Silva, 2020).

Digenea was the third most diverse group (14.3% of the samples found) and was the most recorded group in helminthological survey studies (Pavanelli et al., 1997; Takemoto et al., 2009; Lehun et al., 2020), as these parasites exploit fish as intermediate or definitive hosts in their life cycle. A parasitological survey conducted by Ramos et al. (2013) on infection and distribution of Austrodiplostomum compactum in Brazil, demonstrated that there is a wide range of fish species that are parasitized by A. compactum, encompassing the orders Characiformes, Perciformes, Siluriformes, and Gymnotiformes and, according to Yamada et al. (2008), the parasite presents a wide variety of intermediate hosts, thus presenting low specificity or “preference” to the various intermediate hosts already recorded.

In host fish, the diversity and richness of parasites are influenced by the set of species present in the environment, and most endoparasites are acquired by the trophic route, whereas the habitat, behavior, age, and sex of the hosts are important for ectoparasite infestation (Guidelli et al., 2003). The results obtained from the low prevalence and abundance of some species of endoparasites found in this study are interesting, and according to Overstreet (1997), the absence of parasites in a particular host indicates that the life cycle of the parasite is impaired. In this case, transmission between the mollusk (first intermediate host) and fish (second intermediate host) in the form of cercariae may not occur in this environment, making it impossible to close the cycle.

The Brillouin’s index (HB) calculation results showed two important findings. First, S. borellii, in which parasitic 14 taxa were found, showed a lower HB index than the T. galeatus, in which nine taxa were found (see Table 4). The difference in the HB index is because some species, such as Dactylogyridae gen. sp.1 (Monogenea), A. compactum (Digenea), and Brasergasilus sp. (Copepoda), occurred only once in the S. borellii sample and influenced the value of the diversity index. Second, it may be related to the number of hosts collected; as shown in Figure 2, the sample number of the host S. borellii was not stabilized in the species accumulation curve and probably did not demonstrate its real diversity. Hoplias aff. malabaricus was expected to have the highest parasite composition (19 taxa), mainly if we considered the behavior and trophic level of the host, as addressed by Poulin & Leung (2011).

Species such as Vancleaveus sp. and Trinigyrus sp. (Monogenea) present in T. galeatus and Mymarothecioides sp. (Monogenea) and Brasergasilus sp. (Copepoda) present in S. borellii had their first record of occurrence, but in low abundance and prevalence (below 10%). According to Bush et al. (1990), this type of case can be considered accidental if parasite indices are low. It is also important to highlight that this study is the first record of the occurrence of the remaining species: Urocleidoides ramentacuminatus, U. paradoxus, Rhinoxenus arietinus (Monogenea), Ergasilus cf. bryconis, Gamispinus diabolicus, Gamidactylus jaraquensis and Lernaea devastatrix (Copepoda). Thus, this study contributes to the knowledge of the occurrence of these parasitic species in fish of the families Anostomidae, Erythrinidae, and Auchenipteridae both in terms of their location and geographic distribution, providing new information for future studies on parasite diversity.

Acknowledgements

The authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) – process n. 402670/2016-7, for the support provided to the current study; to Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio), for authorizing the sample collection; to the Environmental Police of Santa Helena County/ PR, for the support provided in scientific expeditions; to the members of Grupo de Estudo em Ictiologia Neotropical (GEIN), and to Universidade Tecnológica Federal do Paraná – UTFPR, Santa Helena Campus, for providing the infrastructure to develop the study.

The authors are also grateful to Núcleo de Pesquisa em Limnologia Ictiologia e Aquicultura-NUPELIA- Universidade Estadual de Maringá-UEM, for providing the infrastructure for this work to be carried out. The authors are also grateful to CAPES for the resources used in this work.

• How to cite: Hasuike WT, Michelan G, Quagliato IS, Brandão H, Takemoto RM. Metazoan parasites of Hoplias aff. malabaricus, Trachelyopterus galeatus and Schizodon borellii (Osteichthyes) from the Protected Area and its main tributary, Brazil. Braz J Vet Parasitol 2023; 32(4): e008323. https://doi.org/10.1590/S1984-29612023055

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Publication Dates

• Publication in this collection
  23 Oct 2023
• Date of issue
  2023

History

• Received
  17 May 2023
• Accepted
  01 Aug 2023