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Arquivos de Neuro-Psiquiatria

Print version ISSN 0004-282X

Arq. Neuro-Psiquiatr. vol.64 no.4 São Paulo Dec. 2006

http://dx.doi.org/10.1590/S0004-282X2006000600029 

Fatal intracerebral hemorrhage secondary to Lonomia obliqua caterpillar envenoming: case report

 

Hemorragia intracerebral fatal causada por acidente com Lonomia obliqua: relato de caso

 

 

Pedro André KowacsI; Juliana CardosoI; Marlene EntresII; Edison Mattos NovakI; Lineu César WerneckI

INeurology Division of the Internal Medicine Department, Hospital de Clínicas, Universidade Federal do Paraná, Curitiba, Brazil
IICenter for Poisoning Control of the State of Paraná Health Office, Curitiba, Brazil

 

 


ABSTRACT

The case of a 70 year-old, previously healthy woman who developed a severe bleeding diathesis shortly after touching a Lonomia obliqua caterpillar and finally died from multiple intracerebral hemorrhages is described. Brain hemorrhages are the leading cause of death in patients envenomed by the Lonomia species. The pertinent literature is reviewed and the most relevant clinical features highlighted, with emphasis on diagnosis. The use of new therapeutic options such as anti-Lonomia serum is discussed.

Key words: intracerebral hemorrhage, Lonomia obliqua, caterpillar envenoming.


RESUMO

O caso de uma mulher de 70 anos, previamente hígida, que desenvolveu diátese hemorrágica grave após contato com uma lagarta Lonomia obliqua, resultando em óbito por hemorragia intracerebral é relatado. Hemorragias cerebrais são uma das causas de morte em acidentes por Lonomia. A literatura pertinente é revisada, sendo as características clínicas e laboratoriais mais relevantes discutidas com ênfase para o diagnóstico, e o uso de novas abordagens terapêuticas como o soro anti-Lonomia.

Palavras-chave: hemorragia intracerebral, Lonomia obliqua, envenenamento, lagartas urticantes.


 

 

First described by Arocha-Pinango and Layrisse in Venezuela in 19671, the hemorrhagic diathesis caused in humans by touching the Lonomia species begins with inflammatory changes at the site of envenoming, followed by systemic symptoms such as headache, fever, vomiting and malaise. After 24 hours, a severe bleeding disorder ensues, leading to echimosis, hematuria, pulmonary and intracranial hemorrhages, and acute renal failure2. Two species of Lonomia are known to cause the hemorrhagic syndrome, Lonomia achelous, found in Venezuela and the north of Brazil, and Lonomia obliqua, found in the south of Brazil1,3,4. Although there are differences in the effect of the venom of both species, both venoms may lead to intense fibrinolytic activity associated with consumption coagulopathy, resembling a diffuse intravascular coagulation5-7. A case of accidental Lonomia obliqua envenoming is described, and its implications are discussed. The report was approved by the institutional regulatory committee.

 

CASE

A 70 year-old, previously healthy woman developed a sudden coma. Four days before, she had started to present hematuria. Shortly after admission, her coma was rated as Glasgow 3. Physical examination revealed several skin hemorrhages, and gross hematuria was present. Based on information in a note left by the patient, two small hyperemic lesions were identified on the tip of her left toe. Along with the note was the the green caterpillar (Fig 1) which was hidden inside of her slipper. CT-scan imaging revealed multiple intracerebral hemorrhages (Fig 2). She died seven days after being envenomed. Her laboratory data is summarized in the Table.

 

 

 

 

 

 

DISCUSSION

Presentation of the symptoms of caterpillar envenoming can generally be classified as follows: a) erucism (a local inflammatory reaction); b) lepidopterism (systemic reactions); c) dendrolimiasis (a chronic form of lepidopterism characterized by dermatitis, migratory inflammatory polyarthritis or polychondritis, chronic osteoarthritis and acute scleritis); d) ophthalmia nodosa (caused by specific families of caterpillars); and e) comsumptive coagulopathy with secondary fibrinolysis8.

The clinical manifestations of Lonomia sp envenoming can include a local inflammatory reaction, which starts immediately after contact; systemic reactions such as headache, fever, vomiting and asthenia, which start some hours after exposure; and a bleeding diathesis, characterized by hematomes and ecchymoses, hematuria, pulmonary hemorrhage, intracerebral hemorrhage and acute renal failure2,7,9.

Due to their greater exposure to the environment, men are more frequently envenomed than women. The site of envenoming usually involves the upper limbs, as the catterpillars live in fructiferous trees4,10.

The severity of the envenoming is related not only to the number of caterpillars involved but also to the intensity of the exposure, since the venom is present not only in the caterpillars’ spines but also in their skin, which consists of a complex tegument with several cuticular specializations such as spicules or scoli11,12. The best characterized toxin in this venom is known by its acronym LOPAP, Lonomia obliqua prothrombin activator protease13.

Lonomia envenoming is frequent in Brazil. In the state of Paraná alone, in the south of Brazil, 354 cases were reported between 1989 and 200514. In the same State, lethality has been decreasing, and fell from 20% in 1995 to 1.5% in 1998, but, interestingly enough, intracranial hemorrhage was confirmed as the leading cause of death in three out of the six fatal cases reported. In the remaining three fatal cases this data was unavailable due to insuffient ancillary support in the rural areas10,14.

Attempts to clarify the underlying mechanisms of intracranial hemorrhage focused on the blood-brain barrier in Wistar rats six hours after exposure to Lonomia obliqua venom. Hippocampal and cerebellar edema were observed, and these resolved 72 hours after the envenoming. Only a single rat out of 28, however, developed intracerebral hemorrhage15. In another study, the venom could not be traced in the brain 6 and 18 hours after administration, but could be found in the kidneys and liver. This evidence suggests that the brain is less vulnerable to LOPAP and that toxins other than LOPAP may also be the cause of brain damage in Lonomia envenoming. It also points to an interaction between both the venom and individual factors15. Indeed, until the present case, intracranial hemorrhages leading to death had only been described in cases of severe envenoming after exposure to multiple Lonomia caterpillars at the same time.

Laboratory findings include a normal platelet count, anemia, a prolonged prothrombin time (PT), and decreased fibrinogen, plasminogen, factor V and factor XIII levels associated with increased fibrin degradation products.

The clinical and laboratory manifestations of envenoming in the case described above are similar to those described in previous reports5,6. There was an increase in the PT and activated partial thromboplastin time (PTT), a reduction in fibrinogen and a mild reduction in the platelet count (Table). Zannin et al.7 also described a significant reduction in coagulation factors V, XIII and VIII, prekallikrein, plasminogen, a-antiplasmin and protein C in patients with fibrinogen levels below 50 mg/dL. They concluded that the hemorrhagic syndrome is the result of consumption coagulopathy and secondary fibrinolisys.

Therapy with whole blood or fresh frozen plasma usually results in a sharp decrease in platelet count, leading to renal insufficiency and death. A more reasonable approach is the administration of antifibrinolytics agents on their own or in combination with cryoprecipitate or purified fibrinogen16. The prognosis is related not only to the severity of the envenoming but also to early intervention.

In the 90’s, Silva et al.17 demonstrated that horses could produce effective antibodies against Lonomia obliqua spine venom. Since then, the antilonomic serum (SaLon) produced at Instituto Butantan has proven effective for treating envenomed individuals17-19 and has been approved by the Brazilian regulatory agency. Caovilla and Barros20 have recently proven its efficacy in 44 envenomed individuals, with either 17.5 mg or 10.5 mg doses. The latter dose was chosen as the ideal one. They also observed that early intervention lead to fewer hemorrhagic complications.

Acknowledgements – The authors express their thanks to Giselia Burigo Guimarães Rubio, Chefe da Divisão de Zoonoses da Saúde do Estado do Paraná, for releasing SESA datafiles for analysis.

 

REFERENCES

1. Arocha-Piñango CL, Layrisse M. Fibrinolysis produced by contact with a caterpillar. Lancet 1969;1:810-812.        [ Links ]

2. Veiga ABG, Pinto AFM, Guimarães JA. Fibrinogenolytic and procoagulant activities in the hemorrhagic syndrome caused by Lonomia obliqua caterpillars. Thromb Res 2003;111:95-101.        [ Links ]

3. Lemaire C. Révision du genre Lonomia Walker (Lep.Attacidae). Ann Soc Entomol France 1972;8:767-861.        [ Links ]

4. Fariha Neto H, Ballarini AJ, Leão RNQ, Costa D Jr , Dias LB. Síndrome hemorrágica por contato com larvas de mariposa (Lepidóptera, Saturnidae). Instituto Evandro Chagas, 50 anos de contribuição às ciências biológicas e à medicina tropical. Fundação SESP 1986;2:811-820.        [ Links ]

5. Arocha-Piñango CL, Blumenfeld-Bosch N, Nouel AL, et al. Fibrinolytic and procoagulant agents from a saturniidae moth caterpillar. In Pirkle H, Markland FS Jr (eds). Hemostasis and animal venoms. Hematology 1988;7:223-240.        [ Links ]

6. Guerrero BAG, Arocha-Piñango CL, Gil A. Lonomia achelous caterpillar venom (LACV) selectively inactivates blood clotting factor XIII. Thromb Res 1997;87:83-93.        [ Links ]

7. Zannin M, Lourenco DM, et al. Blood coagulation and fibrinolytic factors in 105 patients with hemorrhagic syndrome caused by accidental contact with Lonomia obliqua caterpillar in Santa Catarina, Southern Brazil. Thromb Haemost 2003;89:355-364.        [ Links ]

8. Diaz JH. The envolving global epidemiology, syndromic classification, manegement, and prevention of caterpillar envenoming. Am J Trop Med Hyg 2005;72:347-357.        [ Links ]

9. Duarte AC, Crusius PS, Pires CAL, Schilling MA, Fan HW. Intracerebral hemorrhage after contact with Lonomia caterpillars. Lancet 1996; 348:1033.        [ Links ]

10. Rubio GB. Epidemiological surveillance distribution of the caterpillar Lonomia oblique Walker, 1855, in the State of Parana, Brazil. Cad Saúde Pública 2001;17:1036.        [ Links ]

11. Pinto AFM, Dobrovolski R, Veiga ABG, Guimarães JA. Lonofibrase, a novel alpha-fibrinogenase from Lonomia obliqua caterpillars. Thromb Res 2004;113:147-154.        [ Links ]

12. Veiga ABG, Blochtein B, Guimarães JA. Structures involved in production, secretion and injection of the venom produced by the caterpillar Lonomia obliqua (Lepidoptera, Saturniidae). Toxicon 2001;39:1343-1351.        [ Links ]

13. Reis CV, Portaro FC, Andrades SA, et al. A prothrombin activator serine protease from Lonomia obliqua caterpillar venom (Lopap): biochemical characterization. Thromb Res 2001;102:427-436.        [ Links ]

14. Banco de Dados das Notificações de Animais Peçonhentos, SESA/ISEP/ DVPS/CESA/Divisão de Zoonoses e Toxicologia, Curitiba jun 2005.        [ Links ]

15. Silva GH, Hyslop S, Cruz-Höfling. Lonomia obliqua caterpillar venom increases permeability of the blood-brain barrier in rats. Toxicon 2004; 44:625-634.        [ Links ]

16. Arocha-Piñango CL, Guerrero B. Hemorrhagic syndrome induced by caterpillars: clinical and experimental studies. Rev Invest Clin 2003; 44:155-163.        [ Links ]

17. Silva WD, Campos ACMR, Gonçalves LRC, et al. Development of an antivenom against toxins of Lonomia obliqua caterpillars. Toxicon 1996; 34:1045-1049.        [ Links ]

18. Gonçalves MLC, Siqueira-Batista R, Artemenko SR, Gomes AP, Igreja RP. Acidentes por outros animais peçonhentos. In Siqueira-Batista R, Gomes AP, Igreja RP, Huggins DW (eds). Medicina tropical: abordagem atual das doenças infecciosas e parasitárias. Rio de Janeiro: Cultura Médica 2001;149-166.        [ Links ]

19. Gonçalves MLC, Vital ML, Gomes AP, Siqueira-Batista R, Andrade E. Acidentes por outros animais de importância médica. In Siqueira-Batista R, Gomes AP, Santos SS, Almeida LC, Figueiredo CES, Bedoya Pacheco SJ (eds). Manual de infectologia. Rio de Janeiro: Revinter 2003;515-518.        [ Links ]

20. Caovilla JJ, Barros EJG. Efficacy of two different doses of antilonomic serum in the resolution of hemorrhagic syndrome resulting from envenoming by Lonomia obliqua caterpillars: a randomized controlled trial. Toxicon 2004;43:811-818.        [ Links ]

 

 

Received 6 September 2005, received in final form 28 June 2006. Accepted 15 August 2006.

 

 

Dr. Pedro André Kowacs - Serviço de Neurologia / Departamento de Clínica Médica / Hospital de Clínicas da Universidade Federal do Paraná - Rua General Carneiro 181 / 12º andar / sala 1236 - 80060-900 Curitiba PR - Brasil. E-mail: pak@cwb.palm.com.br