Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

Alzate-Marin, Ana Lilia; Baía, Gilson Soares; Martins Filho, Sebastião; Paula Júnior, Trazilbo José de; Sediyama, Carlos Sigueyuki; Barros, Everaldo Gonçalves de; Moreira, Maurilio Alves

doi:10.1590/S0100-84551996000400016

Abstract

RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F1 plants confirmed their status.

RAPD-PCR; hybrid plants; progenitors

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Use of RAPD-PCR to identify true hybrid plants from crosses between closely related progenitors

Ana Lilia Alzate-Marin^I; Gilson Soares Baía^I; Sebastião Martins Filho^I; Trazilbo José de Paula Júnior^I,V; Carlos Sigueyuki Sediyama^I,II; Everaldo Gonçalves de Barros^I,III; Maurilio Alves Moreira^I,IV

^INúcleo de Biotecnologia Aplicada à Agropecuaria (BIOAGRO), Universidade Federal de Viçosa (UFV), 36571-000 Viçosa, MG, Brasil. Send correspondence to E.G.B.

^IIDepartamento de Fitotecnia, UFV, Viçosa, MG, Brasil

^IIIDepartamento de Biologia Geral, UFV, Viçosa, MG, Brasil

^IVDepartamento de Bioquímica e Biologia Molecular, UFV, Viçosa, MG, Brasil

^VEPAMIG, Viçosa, MG, Brasil

ABSTRACT

RAPD-PCR molecular markers were used to identify common bean and soybean hybrid plants derived from crosses between closely related progenitors, with no apparent phenotypic differences. Primers OP-F12 and OP-0O3 were used to identify true hybrids derived from crosses between common bean cultivars Rudá (A 285) and AN 910408, and soybean cultivars Cristalina and Bossier, respectively. Each primer generated one polymorphic DNA band which was present in the male progenitor and absent in the female progenitor. As RAPD bands are normally inherited as dominant characters, the presence of these bands in the F₁ plants confirmed their status.

Keywords: RAPD-PCR; hybrid plants; progenitors.

Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. BRL Focus 12: 13-15.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.

Publication Dates

Publication in this collection
27 Oct 2006
Date of issue
1996

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[1] Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. BRL Focus 12: 13-15.

[2] Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.