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Brazilian Journal of Medical and Biological Research

Print version ISSN 0100-879XOn-line version ISSN 1414-431X

Braz J Med Biol Res vol.33 n.9 Ribeirão Preto Sept. 2000 

Braz J Med Biol Res, September 2000, Volume 33(9) 1027-1036

Eggplant (Solanum melongena) infusion has a modest and transitory effect on hypercholesterolemic subjects

P.R. Guimarães1, A.M.P. Galvão1, C.M. Batista1, G.S. Azevedo1, R.D. Oliveira1, R.P. Lamounier1, N. Freire2, A.M.D. Barros2, E. Sakurai3, J.P. Oliveira4, E.C. Vieira1 and J.I. Alvarez-Leite1

1Laboratório de Nutrição e Gnotobiologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, 2Departamento de Farmacognosia, Faculdade de Farmácia, 3Departamento de Estatística, Instituto de Ciências Exatas, and 4Laboratório Central, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil

Material and Methods
Correspondence and Footnotes


Eggplant (Solanum melongena) is consumed extensively in Brazil. It has been believed that infusion of a powdered preparation of the fruit may reduce serum cholesterol. However, there are few documented reports on its effects on cholesterol metabolism and its possible hypocholesterolemic effect has not been proved by well-controlled studies. The aim of the present study was to observe the effects of S. melongena on the serum cholesterol and triglycerides of 38 hypercholesterolemic human volunteers ingesting S. melongena infusion for five weeks. Thirty-eight hypercholesterolemic subjects receiving either S. melongena infusion (N = 19) or placebo (N = 19) participated in two clinical experiments in which the effect of S. melongena infusion was studied with (N = 16) or without (N = 38) dietary orientation. Total cholesterol and its fractions, triglycerides, and apolipoproteins A and B were measured in blood at the beginning of the experiment and three and five weeks thereafter. No differences were observed compared to control. Intraindividual analysis showed that S. melongena infusion significantly reduced the blood levels of total and LDL cholesterol and of apolipoprotein B. After dietary orientation, no intra- or intergroup differences were seen for any of the parameters analyzed. The results suggest that S. melongena infusion had a modest and transitory effect, which was not different from that obtained with standard orientation for dyslipidemia patients (diet and physical activities).

Key words: cholesterolemia, eggplant, diet, Solanum melongena, atherosclerosis index


Elevations of cholesterolemia and of serum low-density lipoprotein cholesterol (LDL-c) are primary risk factors for cardiovascular disease. Many studies have clearly shown that the reduction of LDL-c levels and the increase of high-density lipoprotein cholesterol (HDL-c) reduce the risk of cardiovascular events and overall mortality (1,2). This fact has led to more aggressive treatment of hypercholesterolemia and to a renewed focus on modification of life style and diet. Eggplant (Solanum melongena) is widely consumed in Brazil and in other countries and its infusion has been used in popular medicine as a hypocholesterolemic agent. However, there are few controlled studies of its effect on cholesterol metabolism, and its possible hypocholesterolemic effect in humans has not been proved by rigorous studies. Mitschek (3) showed that a preparation of S. melongena prevented both hypercholesterolemia and the formation of atheromas in the aorta of rabbits fed high cholesterol diets. However, Kritchevsky et al. (4) did not confirm these findings in rats fed diets containing 1% S. melongena fruit or leaf powders. Recently, Jorge et al. (5) studied the effects of S. melongena on plasma lipid levels, lipid peroxidation and endothelial dysfunction in experimental hypercholesterolemia. The authors fed rabbits a cholesterol-rich diet and administered 10 ml/day of eggplant juice (10:7, whole fruit:water) for 4 weeks. The authors concluded that eggplant juice administered to hypercholesterolemic rabbits significantly reduced weight, plasma cholesterol levels, aortic cholesterol content, and malondialdehyde concentrations in native-oxidized LDL and in the arterial wall and increased endothelium-dependent relaxation.

The aim of the present study was to determine the effects of S. melongena on serum cholesterol and triglycerides of 38 hypercholesterolemic human volunteers ingesting a S. melongena infusion for five weeks. We demonstrated that S. melongena infusion had a discrete, transitory effect in reducing hypercholesterolemia which was similar to that obtained with standard orientation for dyslipidemia (diet and exercise).

Material and Methods

S. melongena analysis

The concentrations of macronutrients (carbohydrates, proteins, lipids and total fiber) and minerals (calcium and phosphorus) were determined in the powder of S. melongena but not in the infusion (especially total fiber) since the concentrations were too low. The pharmacognostic analyses were performed on S. melongena powder and infusion. The presence of chemical classes having therapeutic significance was determined by qualitative standard methods and thin-layer chromatography (for saponin analysis) (6,7). The following groups were investigated: polyphenols, tannins, alkaloids and heterosides of anthracene, flavonoids, saponins (two different methods), and cardiotonics. The aqueous extract was then evaporated at room temperature under a stream of air. The dried residue thus obtained was dissolved in 2 ml of 70% ethanol and this solution was submitted to thin-layer chromatography. For preliminary analyses the plates were prepared with a Vetec 69G F254 silica gel suspension and water. Later, 250-µm-wide Whatman glass plates with silica 60A were used. The specific reagents for saponins used were vanillin/sulfuric acid, anis-aldehyde/sulfuric acid and Kagi-Mischer reagent, the latter being the best.

Clinical experiment

Phase 1: The first phase of this study was a randomized double-blind experiment in which three measurements were made on each subject (at baseline and at three and five weeks after the beginning of S. melongena or placebo administration). The sample size was determined taking this repetition into account, i.e., a positive correlation between the three measurements for a targeted 80% statistical power and a 0.05 significance level. The protocol was approved by the Ethics Committee of the Medical School, Federal University of Minas Gerais, and the subjects gave written informed consent to participate in the study.

Thirty-eight volunteers aged 25-62 years (11 men and 27 women) participated in the experiment. Two of them (one from the placebo group and one from the S. melongena group) did not complete the experiment due to personal or medical reasons not related to it (elective surgery). The criteria for inclusion in the trial were age over 18, borderline or high cholesterol levels (>220 mg%), LDL-c/HDL-c ratio over 2.5, normal or borderline high triglyceride levels (<400 mg%), and no metabolic or uncontrollable disease affecting cholesterol metabolism. Exclusion criteria were introduction or modification of drugs, intolerance to S. melongena or placebo infusions, use of S. melongena infusion before the beginning of the experiment, and interruption of infusion intake for three days or more.

S. melongena was given as a 2% (w/v) infusion prepared as follows: the whole fruit was washed, dehydrated and powdered and 12 g of this powder was sealed in filter paper. Twelve grams of powder corresponded to 100 g of eggplant in natura. Each subject received 35 packets containing 10 g of powdered S. melongena or wheat bran (placebo). They were instructed to prepare the infusion by placing 1 package in 500 ml of hot water for 15 min and to drink it twice a day. The protocol of S. melongena preparation was based on interviews with individuals seen at the University Hospital of UFMG who had used S. melongena on their own initiative to reduce cholesterolemia. Wheat bran was chosen as placebo since it is well known that this fiber has no effect on cholesterolemia. The use of dehydrated, powdered S. melongena was introduced to conserve the fruit during the 5 weeks of the experiment.

At the beginning and after the 3rd and 5th week of the experiment, individuals were interviewed and requested to report on food and life habits, physical activity, previous personal and family diseases, especially dyslipidemia, diabetes mellitus or other metabolic diseases affecting cholesterol metabolism. Body weight, blood pressure and food ingestion were evaluated during each interview. Food ingestion was calculated using food questionnaires previously tested by dietitians and analyzed with a Body Manager software (Kinesis, Belo Horizonte, MG, Brazil). Body mass index (BMI) was calculated by the formula: BMI = weight (kg)/height2 (m). On the 35th day, S. melongena packets that had not been consumed were returned by the patient and computed for the calculation of eggplant infusion intake. After 12 to 14 h of fasting, blood samples were collected without an anticoagulant. The subjects were also instructed to maintain their normal food and life habits, including physical activity, during the experimental period. No dietary recommendations were made during this phase of the experiment. One week after the end of phase 1 all individuals received dietary recommendations to prevent dyslipidemia and were advised particularly to avoid foods with a hypercholesterolemic action, like dairy products, eggs, meat, etc.

Sera were separated by centrifugation immediately after puncture (10,000 g, 10 min). Total cholesterol and triglycerides were determined using the cholesterol oxidase and glycerophosphate oxidase methods, respectively (Labtest kit, Belo Horizonte, MG, Brazil). HDL-c was determined with a Boehringer Mannheim kit (Indianapolis, IN, USA) after selective and quantitative precipitation of LDL-c and very low-density lipoprotein cholesterol (VLDL-c) fractions with phosphotungstic acid. After centrifugation, HDL-c was determined in the supernatant by the cholesterol oxidase method. LDL-c was measured by the polyvinyl sulfate precipitation method (Boehringer Mannheim). LDL-c is calculated indirectly as the difference between total cholesterol and cholesterol in the supernatant after precipitation. VLDL-c was calculated as the difference between total cholesterol and the cholesterol present in the LDL and HDL fractions. Apolipoprotein A-1 (apo A-1) and apo B levels were measured by the immunoturbidimetric method (Sigma diagnostic apo A-1 and apo B reagent kit, respectively; Sigma Chemical Co., St. Louis, MO, USA). Serum alkaline phosphatase and gamma-glutamyl transpeptidase concentrations were determined using commercial kits (Analisa, Belo Horizonte, MG, Brazil).

Phase 2: Three months after the end of phase 1, all volunteers were invited to participate in the second phase of the trial when dietary advice was given. One week after the end of phase 1, all participants had already received instructions about dietary recommendations and physical activity for the treatment of hypercholesterolemia. The objective of this part of the study was to determine the influence of S. melongena infusion on subjects under dietary recommendations for dyslipidemia. The same protocol as described in phase 1 was followed.

Only 16 of 36 individuals were able to participate in this second phase. The individuals who did not participate in phase 2 did so because some started to take hypocholesterolemic drugs, some maintained the use or started (in the placebo group) to use the eggplant infusion, and some could not participate for personal reasons. In order to avoid bias, the volunteers assigned to the placebo group in the first phase received S. melongena during this phase and vice-versa (groups S. melongena2 and placebo2, respectively). Thus, this phase did not represent a transverse study since, in contrast to phase 1 of the experiment, the guidelines related to the treatment of hypercholesterolemia had already been given. The preparation of the experimental and placebo infusions and blood analyses were the same as described for phase 1.

Statistical analysis

The Student t-test was used to analyze the data obtained in phase 1 and phase 2 experiments. The time effect was assessed by the paired Student t-test within each treatment group. Comparisons between treatment group means were made by the Student t-test for independent groups (8). In addition, a longitudinal data analysis approach was adopted for the analysis of the entire data set, comprising both treatment and time effect evaluation (9). Prior to this analysis, a correlation matrix of the covariates was estimated according to Diggle (9). The level of significance was set at 0.05 for all tests except when otherwise stated. The calculations were performed using the SAS-Release 6.11 software (SAS Institute, Cary, 1996), available at the Federal University of Minas Gerais mainframe computer center.


The S. melongena powder contained 15.09% protein, 1.42% total lipids, 13.89% fiber, 0.22% calcium, and 0.31% phosphorus. The qualitative test for composition was positive for polyphenols and negative for anthracenic, flavonic, saponic and cardiotonic heterosides, polyphenols and tannins, as well as tertiary and quaternary alkaloids. However, steroidal saponins were detected when eggplant infusion was analyzed by thin-layer chromatography using Kagi-Mischer developing solution.

The placebo and S. melongena patient groups were homogeneous in terms of age, proper use of S. melongena or placebo and initial blood levels of total cholesterol, HDL-c, LDL-c, VLDL-c, and triglycerides (Table 1). There were no significant differences in body weight, arterial blood pressure or food ingestion for the same subject throughout the experimental period or between the two groups (data not shown). The acceptability of the infusions was quite satisfactory. There were no complaints during the experimental period.

In the first phase of the experiment, no significant differences in serum cholesterol concentrations were observed between groups (Figure 1). The averages were 251 ± 38 and 245 ± 9 mg/dl for the placebo and experimental groups, respectively. However, there was a small but significant difference in total plasma cholesterol levels in individuals in the S. melongena group between the beginning and the end of the 5-week experimental period (P = 0.04). No difference was detected in the placebo group (P = 0.17) (Figure 1). It is interesting to note that these differences were due to reductions in subjects whose plasma levels were higher than 240 mg% (Table 2) and for the period of 0-5 weeks but not 0-3 weeks.

A significant reduction in LDL-c levels was observed between the 3rd and 5th weeks (data not shown). This reduction in LDL-c averaged 13.51% (+14 to -42%) in the S. melongena group, as opposed to 1.5% (+43 to -50%) in the placebo group. There were no variations in blood levels of HDL-c, VLDL-c or triglycerides in subjects from the two groups throughout the experimental period (Table 3). The levels of apo A and apo B were in agreement with the results for LDL-c and HDL-c, i.e., there was a significant reduction in the levels of apo B in relation to the 5th week only in the subjects in the S. melongena group, and similar levels of apo A in both groups (Table 3). The maintenance of HDL-c levels and the reduction of LDL-c levels promoted a significant reduction in the LDL-c/HDL-c ratio in the individuals from the S. melongena group (Figure 2).

The levels of alkaline phosphatase and gamma-glutamyl transpeptidase were the same for both groups, suggesting that the experimental treatment did not affect these tests of hepatic function (Table 3). There was no correlation between serum parameters and the intake of protein, fibers, cholesterol or total, saturated, monounsaturated or polyunsaturated fat (data not shown).

When the initial data obtained for 16 individuals who participated in phase 1 and phase 2 were compared, the initial levels of total cholesterol were found to be significantly lower in phase 2 when compared to initial levels in phase 1 (P = 0.048 and P = 0.013 for placebo2 and S. melongena2 groups, respectively) (Table 4). The initial LDL-c and LDL-c/HDL-c ratio for individuals of the placebo2 group (who received S. melongena infusion in phase 1) were also significantly lower (P = 0.05 and P = 0.032, respectively) and HDL-c values were significantly higher (P = 0.016) in phase 2 compared to initial values in phase 1 (Table 4). This means that volunteers receiving placebo or S. melongena infusion had a reduction in serum cholesterol after the end of phase 1. This reduction was due to the dietary orientation, since no hypocholesterolemic drug treatment was introduced. However, there was no additional serum cholesterol reduction after 3 and 5 weeks of phase 2. Other serum parameters (LDL-c, HDL-c, triglycerides, apoproteins) were also statistically similar between groups at the 3rd and 5th weeks of phase 2 (as determined by paired or unpaired Student t-test). Since all individuals continued the step 1 dietary treatment during phase 2, it can be assumed that the effects of dietary orientation and S. melongena infusion were not additive with respect to serum cholesterol, LDL-c and LDL-c/HDL-c rate.

Figure 1 - Plasma cholesterol of hypercholesterolemic volunteers with no dietary orientation (phase 1), ingesting a 2% infusion of S. melongena or placebo. The points indicate the plasma cholesterol level of each subject before the study and after 5 weeks. Data are reported as means ± SD. P values are given for the paired Student t-test.

[View larger version of this image (8 K GIF file)]

Figure 2 - LDL-c/HDL-c ratio of hypercholesterolemic volunteers with no dietary orientation (phase 1), ingesting a 2% infusion of S. melongena or placebo. The points indicate the plasma cholesterol level of each subject before the study and after 5 weeks. Data are reported as means ± SD. P values are given for the paired Student t-test.

[View larger version of this image (10 K GIF file)]


Although some studies have demonstrated the hypocholesterolemic effect of eggplant in experimental animals, to our knowledge, this is the first controlled study regarding the effects of S. melongena in humans. The data obtained here during phase 1 should be analyzed very carefully since the effect of the ingestion of S. melongena infusion was observed only after intraindividual but not interindividual comparisons. This lack of differences between placebo and S. melongena may probably be due to 1) the small number of individuals in each group, 2) the wide range of cholesterolemia levels within the same group, and 3) the concentration of S. melongena infusion (2%), which may have been lower than that needed to induce hypocholesterolemic effects. However, all positive risk factors, i.e., total cholesterol, LDL-c, LDL-c/HDL-c ratio and apo B levels (10), were reduced by S. melongena ingestion, while HDL-c and apo A, both negative risk factors, were kept at the same levels. Taken together, these results suggest a transient small but significant hypocholesterolemic action of S. melongena infusion but no effect in reducing serum triglycerides. Although Jorge et al. (5) described a hypocholesterolemic effect of eggplant juice in rabbits fed a cholesterol-rich diet, our human experiments using filtered S. melongena infusion cannot be compared to their study since they administered the whole fruit juice (only mixed with water). Moreover, the juice prepared by Jorge et al. was more concentrated (about 58% eggplant) than the S. melongena infusion used in our experiment (2%).

Some vegetables may cause hepatic injuries when administered in large amounts to rats and humans, probably due to antinutritional factors such as tannin or alkaloids (11-13). Under the present experimental conditions, the S. melongena infusions were apparently harmless, as suggested by the normal levels of alkaline phosphatase and gamma-glutamyl transpeptidase in individuals from the S. melongena group.

The possible mechanism of action of S. melongena on cholesterol metabolism has not been clarified. Soluble fibers reduce the levels of blood cholesterol by 5 to 15% in experimental animals and in humans (14,15) through a number of mechanisms. However, it is improbable that soluble fiber is the main hypocholesterolemic component of S. melongena infusions, since the amount of dietary fiber needed for this effect is 15 to 30 g/day (16) and the amount present in 10 g of powder (the quantity used to prepare the infusion) was only 1.3 g. Antioxidants such as vitamin C (ascorbic acid) and phenols have received some credit as preventive agents of atherosclerosis (17,18) but their action in reducing plasma cholesterol levels has been seldom described (18).

Our chemical analysis showed the presence of polyphenols and steroidal saponins in both the powder and infusion of S. melongena. Saponins are a group of glycosides that stimulate the heart and may be found in many vegetables. On shaking, they form foam, a property of the S. melongena infusion. Saponins in amounts as low as 150 mg/kg diet increase the fecal excretion of bile salts in experimental animals and in humans, reducing total cholesterol without affecting the HDL fraction (19-21). Quantification of saponin concentration and other chemical compounds in the infusion of S. melongena is currently under investigation in our laboratory. The presence of steroidal saponins in S. melongena infusion suggests that they may be among the agents responsible for this effect on serum cholesterol. These results are in agreement with those of Paczkowski and Wojciechowski (22), who found steroidal saponins in S. melongena samples. Although flavonoids were not found in our analysis of the infusion, these compounds have also been reported to have some effects on cholesterol metabolism (23,24). Flavonoids have been suggested to be the hypocholesterolemic agents of several vegetables. Flavonoids from the fruits of S. melongena orally administered at a dose of 1 mg 100 g body weight-1 day-1 showed a significant hypolipidemic action on normal and cholesterol-fed rats (23). HMG CoA reductase, lipoprotein lipase and plasma LCAT activity were enhanced. The concentrations of hepatic and fecal bile acids and fecal neutral sterols were also increased, indicating a higher rate of cholesterol degradation. Flavonoids isolated from S. melongena also showed a potent antioxidant activity (24-26). Concentrations of malondialdehyde, hydroperoxides and conjugated dienes were significantly reduced. Catalase activity was found to be significantly enhanced in the tissues of normal and cholesterol-fed rats receiving 1 mg flavonoid from S. melongena 100 g body weight-1 day-1. However, all of these studies used compounds extracted from S. melongena or its whole fruit juice. In our experiment, only the filtered infusion of the dehydrated fruit was ingested. No flavonoid was detected in the infusion. New analyses of S. melongena infusions by nuclear magnetic resonance are under way in our laboratory to detect other chemical compounds which could exert hypocholesterolemic effects.

Independent of its mechanism of action, phase 2 results showed that the effects of S. melongena were not demonstrable after dietary orientation, confirming the efficacy of the conventional dietary treatment for moderate dyslipidemias (27). Three months after the end of phase 1 and of the dietary recommendation, six of 16 individuals had blood cholesterol levels below 220 mg%. It should be pointed out that the results obtained for all 38 individuals during phase 1 (reduction of plasma cholesterol levels in the S. melongena group and no differences in the placebo group) persisted only in these 16 individuals. With respect to the association of S. melongena with traditional diet therapy, the lack of additional beneficial effects may be due to similar mechanisms of action in the two treatments, i.e., the small effect of S. melongena in the reduction of cholesterol absorption may have been masked by the lower cholesterol ingestion. It is interesting to point out that individuals in the placebo2 group (i.e., subjects who had ingested the S. melongena infusion during phase 1 and who followed dietary orientations) had lower risk factors (lower total cholesterol and LDL-c, higher HDL-c) at the beginning of phase 2 compared to the beginning of phase 1 (Table 4).

We conclude that a 2% infusion of S. melongena has a slight effect on the reduction of cholesterolemia and, most importantly, reduces LDL-c with no alteration in HDL-c. Steroidal saponins could be involved in the modest hypocholesterolemic effect of S. melongena infusion, since they were detected in both powder and infusion of S. melongena. However, this effect does not seem to add anything to the widely accepted conventional dietary treatment for hypercholesterolemia.


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The authors thank Prolacteos Ltda. for providing the Solanum melongena powder.

Correspondence and Footnotes

Address for correspondence: J. Alvarez-Leite, Departamento de Bioquímica e Imunologia, ICB, UFMG, Caixa Postal 486, 30161-970 Belo Horizonte, MG, Brasil. Fax: +55-31-441-5963. E-mail:

Research carried out at Laboratório de Nutrição e Gnotobiologia, Departamento de Bioquímica e Imunologia, ICB/UFMG, and Ambulatório Bias Fortes, Hospital das Clínicas, Faculdade de Medicina, UFMG. Research supported by CNPq and FAPEMIG. Received May 22, 1999. Accepted April 10, 2000.

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