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Food Science and Technology

Print version ISSN 0101-2061On-line version ISSN 1678-457X

Food Sci. Technol (Campinas) vol.35 no.1 Campinas Jan./Mar. 2015 

Research Paper

Vinegar rice (Oryza sativa L.) produced by a submerged fermentation process from alcoholic fermented rice

Wilma Aparecida Spinosa 1   * 

Vitório dos  Santos Júnior 1  

Diego Galvan 1  

Jhonatan Luiz Fiorio 1  

Raul Jorge Hernan Castro Gomez 1  

1Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Estadual de Londrina – UEL, Londrina, PR, Brazil


Considering the limited availability of technology for the production of rice vinegar and also due to the potential consumer product market, this study aimed to use alcoholic fermented rice (rice wine (Oryza sativa L.)) for vinegar production. An alcoholic solution with 6.28% (w/v) ethanol was oxidized by a submerged fermentation process to produce vinegar. The process of acetic acid fermentation occurred at 30 ± 0.3°C in a FRINGS® Acetator (Germany) for the production of vinegar and was followed through 10 cycles. The vinegar had a total acidity of 6.85% (w/v), 0.17% alcohol (w/v), 1.26% (w/v) minerals and 1.78% (w/v) dry extract. The composition of organic acids present in rice vinegar was: cis-aconitic acid (6 mg/L), maleic acid (3 mg/L), trans-aconitic acid (3 mg/L), shikimic + succinic acid (4 mg/L), lactic acid (300 mg/L), formic acid (180 mg/L), oxalic acid (3 mg/L), fumaric acid (3 mg/L) and itaconic acid (1 mg/L).

Key words: submerged fermentation; microfiltration; acetic acid bacteria; vinegar

1 Introduction

Vinegar is a product used worldwide. Despite its still unknown origin, vinegar is now familiar to diverse cultures, and is consumed indiscriminately by all social classes. It is used as a condiment, for flavoring, as a preservative, as a medicine for routine use, and even as a cleaning agent (Qiu et al., 2010; Budak et al., 2014; Haruta et al., 2006; Baena-Ruano et al., 2006).

Rice vinegar and vinegar produced from other raw materials are widely used by Asian populations (Chen & Chen, 2009; Xiao et al., 2011). In Japan, the acceptance of the product has reached the point where shops and bars specialize in commercial vinegar, even using refinements usually reserved for other products, such as aging in wooden casks (Haruta et al., 2006; Nakamura et al., 2010). As in the Japanese market, the established presence of vinegar in China has also led to the search for alternatives to trade the product, such as the launch of beverages with functional appeal based on rice vinegar (Chen & Chen, 2009; Chen et al., 2012; Fan et al., 2009).

In Europe, where vinegar also assumes other functions, i.e. as a disinfectant, degreaser and odor neutralizer, per capita consumption reaches 1.8 L/year (Budak et al., 2014; Blankart, 2013). In Brazil, although less widespread and generally restricted to use as a seasoning, vinegar consumption is rooted in the culture of the country; the average Brazilian consumes 0.8 L/year (Blankart, 2013; Solieri & Giudici, 2009). The use of vinegar in Brazil has increased, due to the increase in purchasing power, which has fostered the presence of imported vinegars, mainly from European countries. In the last decade, the competitive landscape has led most companies to offer special vinegars to the market (Rizzon & Miele, 1998; Bortolini et al., 2001).

There are three main processes for the microbiological conversion of a dilute solution of ethanol (wine) in vinegar: the slow process (Orleans or French), the rapid process (or German) and the submerged process (Budak et al., 2014; Mas et al., 2014).

The term "submerged acetic fermentation" is analogous to the usual procedures involved in antibiotic production and the cultivation of yeasts. In this case, bacteria execute fermentative work on a fluid, which is the alcohol mixture. This is accomplished without contact material, such as wood chips or coal, which are used in processes with generators (the rapid process). Bacteria are always submerged in the liquid to ferment, where they multiply and oxidize the alcohol mixture into vinegar (Budak et al., 2014; Mas et al., 2014; Schlepütz et al., 2013).

In this process, acetic acid bacteria are submerged in the liquid to ferment, multiplying and drawing power from the oxidation of ethanol to acetic acid. To catalyze the reaction that gives them energy, acetic acid bacteria require an adequate and continuous supply of oxygen in all parts of the tank. An interruption in the oxygen supply, especially in the final stages of fermentation, will affect the performance (Budak et al., 2014; Mas et al., 2014). In view of the favorable consumption of vinegar, this work invested in an alternative for the production of rice vinegar by submerged fermentation.

2 Materials and methods

2.1 Rice vinegar

The rice wine (Oryza sativa L.) used for vinegar production had an alcohol concentration of 6.28 g/100 mL, density 20º/20°C of 0.9890, glucose content of 2.8 g/100 mL and the pH was 3.00.

2.2 Microorganism Acetobacter sp

In the fermentation step, Acetobacter sp, obtained from the company Frings Microdyn of Brazil (Piracicaba-SP), consisting of the natural contaminant alcoholic musts, were used in the presence of air. Such bacteria are selected spontaneously, depending on the acidity conditions of the medium in which they are kept.

2.3 Acetic fermentation

The Acetator® (Frings, Germany), used for rice vinegar production in submerged fermentation at the pilot scale, was obtained from Frings Microdyn of Brazil. The total volume of the Acetator® is 8.0 L, with working volume of 6.0 L, consisting of a transparent reaction vessel with cooling coils and turbine aeration, a foam rupture disc, probes for temperature measurement and manometry, and valves to control loading, unloading and cooling. The equipment has a height two times greater than its diameter. Inside the reactor, there is a cooling coil, whose function is to dissipate the heat generated by the exothermic reaction of fermentation and heat transfer from the motion of the aerator motor. The cooling water of the reactor is controlled by instrumentation to maintain a temperature oscillation of ±0.3°C (Qi et al., 2014).

During the process, the total acidity, alcohol content and aeration were managed, as the acidity and alcohol content are the determining parameters for loading and unloading the fermentation cycles. The total concentration (TC) of the wine was given by the sum of the concentrations of ethanol and the total acidity (Adams & Moss, 2008). Starting with 3.4 L of wine, with an alcohol content of 6.28% (w/v), plus a 1.6 L inoculum of Acetobacter sp, this contained a mass of 2.7 g/L of bacteria determined by measuring the optical density at 600 nm.

The wine fermentation process was followed for 10 cycles. The submerged acetic fermentation process was conducted as a fed batch. When the alcohol content approached 0.5%, 1/3 of the vinegar volume was withdrawn from the reactor and the same volume of wine was added.

The rice wine was adjusted to 0.5 g/L nutrient salts. The composition of salts and mineral nutrients included diammonium phosphate, potassium sulfate, magnesium sulfate, concentrated bacterial growth factors and vitamins. The fermentation temperature was set at 30±0.3°C and was automatically controlled by the equipment. The air flow rate was maintained at around 30 L/h. The alcohol content was determined at the beginning and at the end of each fermentation cycle, while the acetic acid content was monitored at shorter intervals.

The velocity control of transformation of alcohol into acetic acid allowed the programming time of unloading and reloading of the automatic Acetator®, so that the alcohol content was not less than 0.2% (w/v). The relationship between the amount of mash fermentation removed and that remaining in the Acetator® was 1/3:2/3, thus maintaining the highest degree of acidity and avoiding too little alcohol.

2.4 Vinegar filtration

The system for crossflow microfiltration was composed of microporous polypropylene tubes with size of 0.2 μm. The capillary diameter was between 1.8 and 2.6 mm. The tubes were bonded in modules and the surface of the membrane filter was 0.04 m2.

The vinegar not filtered was pumped continuously and recirculated inside the capillaries. Only a small portion of fluid crossed the membrane wall of the capillaries, leaving the module free of the bacteria contained in the crude vinegar.

2.5 Yield

The yield of acetic acid (ηaa) was calculated as the ratio of the mass of acetic acid produced (g/100 mL) and initial ethanol (g/100 ml). The stoichiometric yield in the conversion of ethanol to acetic acid is 0.77. The productivity of acetic acid (Paa) was calculated according to Equation 1.

Paa=(vaa produced L x content of aa (g/L))(reactor volume L x fermentation time of the cycle h)  (1)

2.6 Analytic methods

The alcohol content (% v/v) was determined using a digital densimeter (Anton Paar, Austria, model DMA4500M). The total acidity was determined by titration with 0.1 M sodium hydroxide and an alcoholic solution of phenolphthalein as the indicator (Brasil, 2005).

The volatile compounds present in rice wine used as raw material for the manufacture of vinegar were determined by gas chromatography with flame ionization (GC-FID) (Agilent, United States, Model 6890). A DBWAX column was used (30 m by 0.25 mm with a film thickness of 0.25 mm). The column temperature was 120°C and the detector temperature was 200°C. N2 was used as the carrier gas with a flow of 20 mL/min.

For the characterization of rice vinegar, the dry contents obtained at 100°C and the ash extract were assessed by the methodology prescribed by Brazilian law (Brasil, 2005). The analysis of these organic acids was based on the use of high performance liquid chromatography (HPLC) (Agilent, United States, Model 1050). A Bio-Rad Aminex (HPX-87H) column was used for organic acids, with dimensions of 300 mm by 7.8 mm. The mobile phase was 5 mM sulfuric acid, the injection volume was 20 µL at a temperature of 55°C and the detector used was of refractive index.

3 Results and discussion

The rice wine used as a substrate for acetic fermentation showed fractions of esters, ketones, alcohols and aldehydes; these are shown in Table 1.

Table 1 Characterization of the rice wine used as a substrate for acetic acid fermentation. 

Determination mg/100 mL
Acetal 1.38
Acetaldehyde 16.70
Ethyl acetate 0.05
Acetone 0.30
Ethanol 5592.24
Glycerol 650.00
Isoamylic 30.80
Isobutanol 4.78
Methanol 0.05
n-Butanol 0.05
n-Propanol 2.08

Aldehydes, ketones and alcohols are limiting factors in the inhibition of Acetobacter sp. In the submerged fermentation process, acetic acid bacteria have the ability to adapt smaller with inhibiting agents than those of the rapid acidification process. The industry standard recommends that the amounts of esters such as ethyl acetate in the alcohol to be used for acetic fermentation should not exceed 0.2 g/100 mL, and methanol should not be present at levels greater than 0.035 g/100 mL (Fortes et al., 2012; Reddy et al., 2008). These compounds have an inhibitory effect on acetic acid fermentation. Furthermore, methanol is toxic when present at levels above this level in fermented drinks (Dato et al., 2005). Glycerol was at a relatively low concentration (0.65 g/100 mL), which is a negative aspect, since this compound gives the wine a soft and sweet taste when in the range of 7-9 g/L (Clarke & Bakker, 2008).

The results concerning acetic acid fermentation for each cycle are listed in Table 2. Columns 2 to 7 show the parameters of the feeding of each cycle. The others are related to the volume of the product and its features.

Table 2 Monitoring acetic acid fermentation from rice wine conducted in an Acetator® autopilot 8 L. 

Cycle at
(h) (°C) (L/h) (L) (%) (%) (%) (L) - (%) (%) (%) (%) (g/L/h)
24.3 22.0 5.0 5.3 3.2 8.4
1 54.0 0.7 2.6 8.6 0.4 9.1 105.9 0.140
26.2 30.0 5.9 6.3 1.4 7.7
2 34.0 2.3 2.6 7.8 0.3 8.1 95.1 0.656
25.3 35.0 6.2 5.3 2.1 7.4
3 33.5 2.3 2.6 7.3 0.4 7.8 89.9 0.629
28.3 35.0 6.1 4.7 2.6 7.3
4 28.0 2.3 2.6 7.4 0.3 7.6 90.2 0.756
29.3 30.0 5.8 5.4 1.8 7.2
5 21.0 2.3 2.7 7.0 0.3 7.2 85.5 0.954
30.2 30.0 6.0 5.4 1.8 7.2
6 23.0 2.3 2.7 7.1 0.2 7.2 86.4 0.881
29.8 32.0 6.0 5.5 1.6 7.1
7 24.5 2.3 2.7 7.0 0.2 7.2 86.2 0.825
30.1 32.0 6.0 5.3 1.7 7.1
8 40.5 2.3 2.7 7.0 0.2 7.2 86.1 0.498
29.8 32.0 5.9 4.8 2.2 7.0
9 30.0 2.3 2.7 6.8 0.3 7.1 83.7 0.655
29.7 30.0 6.4 4.8 2.1 6.9
10 25.0 2.3 2.7 6.6 0.4 7.0 80.6 0.756
mAverage 28.2±6.39 28.7±1.70 32.0±1.93 6.03±0.17 5.3±0.46 2.0±0.34 7.2±0.22 2.3±0.0 2.66±0.05 7.1±0.35 0.3±0.8 7.4±0.35 88.2±4.49 0.73±0.14

a fermentation time;

b air flow;

c volume of liquid in the Acetator®;

d wine acidity;

e ethanol in wine;

f total concentration in the Acetator®;

g volume of produced vinegar;

h vinegar acidity;

i ethanol in vinegar;

j total concentration in vinegar;

k ηaa: yield;

l Paa: productivity.

m average from cycle 2 to 10 (8 cycles).

From the second cycle onwards, the fermentation process was stable regarding the time of fermentation, the acetic acid content in the product, yield and productivity. All averages were calculated neglecting the first cycle. It can be inferred that this type of fermentation, i.e. fed batch and using an automatic Acetator®, provided satisfactory performance for the production of vinegar.

Figure 1 shows the total acidity in the vinegar obtained from the fermentation process from the second to the tenth cycle. The average value of total acidity of the vinegar obtained from the second to the tenth cycle was 7.11±0.35%, expressed as acetic acid (w/v), with a variation coefficient of 0.05.

Figure 1 Total acidity of the rice vinegar fermented in the Acetator®

The acetic acid concentration in the fermented product varied from 6.6 to 7.8% (w/v). This value indicates a high rate of conversion of ethanol to acid, as the substrate (rice wine) had an alcohol concentration of 6.28% (w/v). The product meets the minimum requirements established by Brazilian law, which defines vinegar as having a minimal volatile acidity of 4 g/100 mL, expressed as acetic acid (Brasil, 2009). This value indicates that the product can almost be classified as double vinegar, where the fermented alcoholic vinegar must provide a volatile acidity exceeding 8 g of acetic acid per 100 mL of the product (Brasil, 2012).

The rice wine used as a raw material for rice vinegar had a pH of 3.0, while the average pH of the product amounted to 2.66 ± 0.05 with a variation coefficient of 0.02, from the second to the tenth cycle of fermentation (Figure 2). Tessaro et al. (2010), in a fermented orange process, worked with orange wine with a pH from 3.55 to 3.68. Bortolini et al. (2001) reported pH values for kiwi wine close to 3.6. According to Adams (1998), acetic acid levels of 7% in the vinegar give a pH value close to 3.

Figure 2 pH of rice vinegar fermented in the Acetator®

In this study, the average time for the acetification of rice wine was 28.8±6.28 h, with a coefficient of variation of 0.22, to achieve an average total acidity of 7.1% acetic acid (w/v). The process for the production of vinegar by submerged culture was capable of fermenting the alcohol substrate thirty times faster than any other process (fast or slow) (Moretto, 1988).

This rapid fermentation was demonstrated by the results obtained at each fermentation cycle (Table 2). These values are considered technically adequate for acetic acid fermentation. Silva et al. (2007) showed that 50 h was required to reach the maximum concentration of acetic acid from fermented cashews. Bortolini et al. (2001) obtained an oxidation time for ethanol from kiwi acetic acid of 12 h in a submerged process and 24 h for a generator process. Nanba et al. (1985) optimized the process of slow fermentation for the production of vinegar and obtained 3% of acetic acid in the product, with a fermentation time of 100 h.

Figures 3 and 4 present the results for the yield and productivity obtained in nine cycles from the second to the tenth fermentation cycle.

Figure 3 Conversion efficiency of rice wine to rice vinegar conducted in the Acetator®

Figure 4 Productivity of rice vinegar conducted in Acetator®

The converted amount of alcohol, with a concentration of 6.28% (w/v) acetic acid, was in the range from 80.6 to 95.1%, with an average over nine cycles of 88.2 ± 4.49% and a coefficient of variation of 0.05, obtained from the second to the tenth fermentation cycle. Bortolini et al. (2001) presented yields ranging from 93.24 to 98.34% for the submerged process and around 82.35% for the generator process, when kiwi wine was used with ethanol content between 3.54% and 9.61% (w/v).

The productivity average of rice vinegar was 0.7± 0.14 g/L/h of acetic acid. Silva et al. (2007), when investigating the production of cashew vinegar, obtained a productivity of 0.55 g/L/h. The authors started from syrup with an initial total concentration of 5.8% (4.5% ethanol and 1.0% acetic acid in cashew wine). Bortolini et al. (2001) compared the submerged and generator processes, and obtained productivity values of 0.29 to 1.73 g/L/h, respectively. Zancanaro (1988), studying the optimization of the slow process of acetic fermentation, reported that this process can achieve a productivity of 0.038 g/L/h for acetic acid, if the recommendations advocated in his study are followed. Despite the differences between the slow and submerged processes, and comparing the data obtained from both, there is far greater productivity with the submerged process in relation to the slow or generator process.

The efficiency of the submerged process is directly linked to the oxygenation of the medium and the state of division of the air bubbles. The Acetator® autopilot allowed for achieving the reported results. The most important component of the reactor was the aeration system. With the pressure exerted by the rotation of the turbine, the aerator inhaled ambient air. This, in turn, was filtered before passing through the suction tube and be introduced, in a downward motion, into the stator, then blended with the liquid contained within the vessel.

The aeration system enabled the production of air microbubbles, with a diameter of 1 to 2 mm and flow rates of 0.3 to 0.4 volumes of air per volume of medium per minute. The microbubbles were introduced into the bottom of the fermenter by means of aerating turbines causing intense agitation in the medium. The retention time was optimized, since the microbubbles tended to rise more slowly than the larger bubbles. Thus, an excellent dissolved oxygen profile was produced in the fermenter. Microbubbles have greater surface contact with the liquid, reaching in situations of homogeneous fermentation, without turbulence or dead zones, a value of up to 80% dissolved oxygen. Another important factor during the fermentation process is that air consumption is minimized, since it reduces the loss of raw materials by evaporation and prevents the formation of foam, providing a stable fermentation medium (Schlepütz et al., 2013; Qi et al., 2014).

The product of acetic acid fermentation (vinegar) was clear and had no deposits. The results obtained from the evaluation of the quality and identity of rice vinegar are shown in Table 3.

Table 3 Composition of rice vinegar. 

Components % (w/v)
Acidity 6.85
Ethyl alcohol 0.17a
Minerals 1.26
Dry extract 1.78

a (w/w)

Brazilian law does not have standards for the identity and quality of rice vinegar; regulations only exist for wine vinegars, fruit, cereal alcohol and alcohol. Based on the quality standards required for the types of vinegars mentioned, the rice vinegar produced in this study is in accordance with such norms. The main standard of quality is the content of acetic acid (Brasil, 2012). Acidity by itself determines if the product is a vinegar or diluted acetic acid. Several tests have been proposed, but have high cost. A simple method, not yet fully reliable, is the measurement of pH. Non-volatile buffering substances have higher pH values than those observed in acetic acid solutions with equivalent concentrations (Adams, 1998).

Table 4 shows the composition of organic acids present in the vinegar.

Table 4 Composition of organic acids present in rice vinegar. 

Components mg/L
Acetic acid 61500
Cis-aconitic acid 6
Maleic acid 3
Trans-aconitic acid 3
Succinic+shikimic acids 4
Lactic acid 300
Formic acid 180
Oxalic acid 3
Fumaric acid 3
Itaconic acid 1

The secondary constituents of vinegar are important because of their contributions to flavor and aroma. These components are characteristic of each substrate used in alcoholic and acetic fermentation. With aging, the vinegar becomes softer and more pleasant to taste and smell, due to reactions such as esterification. These esterification reactions remove the acids and alcoholic groups from the medium, providing aromatic esters, and oxidation oxidizes aldehyde groups, conferring ruggedness to the vinegar (Reddy et al., 2008; Adams, 1998).

4 Conclusion

The results obtained under the experimental conditions and with the equipment described above provided vinegar from rice wine, with a productivity of 0.7 g/L/h, 88.2% yield and 7.1% total acidity (w/v) in acetic acid; the average was evaluated over nine fermentation cycles. The Acetator® autopilot contributed decisively to the final results regarding productivity and the acetic acid content. The product of acetic acid fermentation (vinegar) was clear and showed no deposits due to the tangential microfiltration equipment used.


The authors thank the State University of Londrina (UEL), CNPq for a scholarship as well as Gerhard Feigl and Frings Microdyn from Brazil.

Practical Application: Vinegar rice produced by a submerged fermentation process from alcoholic fermented rice.


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Recebido: 06 de Janeiro de 2015; Aceito: 25 de Fevereiro de 2015

*Corresponding author:

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