Abstracts
This study assessed the effects of different levels of oldman saltbush hay and ageing time on the physical characteristics of Santa Inês lamb meat. Sixty shoulders from 32 male lambs fed with 30, 40, 50 or 60% oldman saltbush hay for 60 days were vacuum-packaged and stored in a refrigerator at 0 ± 1°C for 0, 7 or 14 days of ageing. The shear force, cooking loss and water holding capacity were 3.06kgf cm-2, 37.28% and 76.71%, respectively, and there were no significant changed by studied factors (P>0.05)
cooking loss; tenderness; ageing; sheep
Objetivou-se com este trabalho avaliar o efeito da inclusão de diferentes níveis de feno de erva-sal e tempo de maturação sobre as características físicas da carne de cordeiros Santa Inês. Sessenta paletas apartir de 32 cordeiros machos, alimentados com 30, 40, 50 e 60% de feno de erva-sal por 60 dias foram embaladas a vácuo e mantidas sob refrigeração a 0±1oC por 0, 7 e 14 dias de maturação. A força de cisalhamento, perda por cocção e capacidade de retenção de água não foram influenciados (P>0,05) pelos fatores estudados, apresentando valores médios de 3,06kgf cm-2, 37,28% e 76,71%, respectivamente
perda por cocção; maciez; maturação; ovinos
INTRODUCTION:
The concept of meat quality is dynamic and evolves with consumer preferences. Meat
quality also involves many characteristics that are strongly linked to regional
traditions and cultures, thus making impossible to create a universally accepted
definition. A quality product should meet the expectations of consumers, i.e., a
healthy, nutritious and tasty food product (ROTA et al.,
2004ROTA, E.L. et al. Efeitos do cruzamento de carneiros da raça Texel com
ovelhas Corriedale e Ideal sobre a qualidade da carne. Revista brasileira de
Agrociência, v.10, n.4, p.487-491, 2004. Available from:
<http://www.ufpel.tche.br/faem/agrociencia/v10n4/artigo14.pdf>. Accessed: Oct.
15, 2011.
http://www.ufpel.tche.br/faem/agrocienci...
). To meet the requirements of the consumer market, the production sector
must study the factors that influence the physical and chemical characteristics of the
meat because these characteristics determine its quality and acceptability (MARTÍNEZ-CEREZO et al., 2005MARTÍNEZ-CEREZO, S. et al. Breed, slaughter weight and ageing time
effects on consumer appraisal of three muscles of lamb. Meat Science, v.69,
p.795-805, 2005. Available from:
<http://www.sciencedirect.com/science/article/pii/S0309174004002955>. Accessed:
Feb. 05, 2012. doi: 10.1016/j.meatsci.2004.11.013.
http://www.sciencedirect.com/science/art...
).
Using alternative foods for sheep finishing can minimize animal production costs and
improve producer profits, which help to sustain animal production systems (MORENO et al., 2010MORENO, G.M.B. et al. Desempenho de cordeiros Santa Inês alimentados com
níveis de feno de erva-sal e concentrado. In: Reunião Anual da Sociedade Brasileira
de Zootecnia, 47., 2010, Salvador, BA. Anais... Salvador: Sociedade Brasileira de
Zootecnia, 2010. (CD-ROM).). Due to its potential as feed,
resistance to diseases, resistance to pests and ease propagation, oldman saltbush
(Atriplex nummularia Lindl.) has been used in various regions as a major
feed source. Atriplex is well adapted to salt and drought stress (ABOU EL NASR et al., 1996ABOU EL NASR, H.M. et al. Value of processed saltbush and acacia shrubs
as sheep fodders under the arid conditions of Egypt. Small Ruminant Research, v.24,
p.15-20, 1996. Available from:
<http://www.sciencedirect.com/science/article/pii/S0921448896009327>. Accessed:
Mar. 22, 2014. doi: 10.1016/S0921-4488(96)00932-7.
http://www.sciencedirect.com/science/art...
) and high levels of
protein in A. nummularia hay (20.2%) (MENESES et al., 2012MENESES, R. et al. Evaluating the use of Atriplex nummularia hay on feed
intake, growth, and carcass characteristics of creole kids. Chilean Journal of
Agricultural Research, v.72, n.1, p.74-79, 2012. Available from:
<http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000100012>.
Accessed: Mar. 22, 2014. doi: 10.4067/S0718-58392012000100012.
http://www.scielo.cl/scielo.php?script=s...
) and crude fiber (22.2%) (ABU-ZANAT, 2005ABOU EL NASR, H.M. et al. Value of processed saltbush and acacia shrubs
as sheep fodders under the arid conditions of Egypt. Small Ruminant Research, v.24,
p.15-20, 1996. Available from:
<http://www.sciencedirect.com/science/article/pii/S0921448896009327>. Accessed:
Mar. 22, 2014. doi: 10.1016/S0921-4488(96)00932-7.
http://www.sciencedirect.com/science/art...
) may be found.
One of the best traits of saltbush is its capacity to grow and produce biomass in arid,
salty environments. Once established the plant is resistant and can remain productive
for several decades. High levels of protein, vitamin E and sulfur in oldman saltbush
plants supplement old and low-quality pastures. The main limitations of saltbush are its
high salt and oxalate levels in addition to its moderate energy values and low biomass
production levels. Adding saltbush to complimentary plants, such as cactus forage, is a
viable solution for improving animal performance, but this solution assumes that the
additional plants can grow in salty soil or be established with saltbush, which is not
true. The presence of vitamin E, minerals and antioxidants can also help to improve meat
quality (BEN SALEM et al., 2010BEN SALEM, H. et al. Potential use of oldman saltbush (Atriplex
nummularia Lindl.) in sheep and goat feeding. Small Ruminant Research, v.91, p.13-28,
2010. Available from:
<http://www.sciencedirect.com/science/article/pii/S0921448810000246>. Accessed:
Out. 15, 2011. doi: 10.1016/j.smallrumres.2009.10.017.
http://www.sciencedirect.com/science/art...
).
Ageing is a meat tenderizing technique that is widely used because it is simple and efficient. In addition, meat tenderizing is a natural process driven by the enzymes in the meat and does not require the use of chemicals. Therefore, this study evaluated the physical characteristics of shoulder meat aged for 0, 7 or 14 days from male lambs that were fed with diets containing 30, 40, 50 or 60% saltbush hay.
MATERIALS AND METHODS:
Sixty shoulders were used from 32 uncastrated male Santa Inês sheep raised in feedlot and fed with 30, 40, 50 or 60% of oldman saltbush combined with concentrate feed at different hay:concentrate ratios. The animals were kept under experimental conditions for 60 days, which was preceded by 20 days of diet and care adaptation. Diets were formulated using the requirements recommended by the NATIONAL RESEARCH COUNCIL (2006)NATIONAL RESEARCH COUNCIL (NRC). Nutrient requirement of small ruminants. Washington, DC, 2006. 362p. to achieve the nutritional requirements of lambs with 20kg of body weight and a daily weight gain of 200g animal-1. Diet and mineral supplementation were provided ad libitum. The diet contained 89% DM, 12% CP and 4Mcal kg-1 crude energy.
After 60 days of feedlot and average 10 months of age, the animals were weighed to obtain the final body weight. The average final weights of lambs fed with 30, 40, 50 or 60% saltbush hay were 36.31±3.41, 34.88±5.07, 32.75±4.24 and 31.06±4.40kg, respectively. The animals were held in fasting for 18 hours and slaughtered by electric stunning (330V about 12 seconds) followed by bleeding. The carcasses were refrigerated at 5±2°C for 24 hours. At 24 hours post mortem, the shoulder muscles were collected using a scalpel, labeled, vacuum-packed and stored in refrigeration at 0±1°C for 7 and 14 days. For day 0 (without ageing), the shoulders were frozen at -18°C immediately after obtaining the meat cuts.
After ageing the shoulder muscles, pH was measured using a digital pH meter connected to a penetrating probe. Color was measured using a Minolta CR-300 colorimeter (diffuse illumination/0° viewing angle, specular component included) and the CIELAB system (L*, lightness; a*, redness; and b*, yellowness) calibrated to a white standard.
Water-holding capacity was calculated using the method described by HAMM (1961)HAMM, R. Biochemistry of meat hydration. Advanceds in Food Research,
v.10, p.335-443, 1961. Available from:
<http://www.sciencedirect.com/science/article/pii/S006526280860141X>. Accessed:
Jan. 10, 2012. doi: 10.1016/S0065-2628 (08) 60141-X.
http://www.sciencedirect.com/science/art...
with slight modifications. Meat samples
weighing 500±20mg were placed on filter paper between two acrylic plates, and a 10kg
weight was placed on top of the plates for 5 minutes. The results are expressed as
percentages compared to initial weight as follows:
WHC=100-((Wi-Wf)/Wi*100), where WHC is the
water-holding capacity, Wi is the initial weight, and Wf is the
final weight.
To measure cooking loss, the samples were weighed and cooked in an industrial oven preheated to 175°C until the internal temperature of the samples reached 72°C. The samples were then removed from the oven, cooled at room temperature and weighed again. Cooking loss was calculated by the difference between the initial weight and final weight of the cooked samples. Subsequently, subsamples were taken from the cooked samples parallel to the muscular fibers while avoided connective tissue and fat. Samples were cut perpendicular to the muscular fibers. The heights and widths of the subsamples were measured to calculate the area in cm2, and the shear forces of the subsamples were measured using a TA-TX2 Texture Analyzer connected to a Warner-Bratzler device. The data were expressed in kgf cm-2.
Data were analysed using the AGROESTAT program (BARBOSA & MALDONADO JR, 2010BARBOSA, J.C.; MALDONADO JR, W. AgroEstat. Sistema para análises estatísticas de ensaios agronômicos. Versao 1.0. Universidade Estadual Paulista "Júlio de Mesquita Filho" - Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, Brasil, 2010. ). The experimental design was a 3×4 factorial design (three ageing times and four levels of saltbush hay), with four shoulders per treatment to SF and CL and five shoulders per treatment to pH, WHC and colour measurements. Analysis of variance and comparisons of the averages using Tukey's test at a 5% probability level. Polynomial regression analysis was used to evaluate the effect of the saltbush hay levels.
RESULTS AND DISCUSSION:
There was no interaction between the saltbush hay level and ageing (P>0.05) for pH,
a* and b* values (Table 1). Shoulders aged for 14 days had lower pH values (5.66) than aged for
7 days (5.78). The average pH values were higher (P<0.05) in meat from animals fed
with 60% saltbush hay (5.76) but did not differ from the pH values of meat from animals
fed with 30 (5.73) or 50% (5.69) saltbush hay diets. According PARDI et al. (2001)PARDI, M.C. et al. Ciência, higiene e tecnologia da carne. 2.ed.
Goiânia: UFG, 2001. 623p., pH values ranging from 5.5 to 5.8 are normal 24
hours after slaughter. In this study, the pH values of the shoulder muscles were within
this range GONÇALVES et al. (2004)GONÇALVES, L.A.G. et al. Efeitos do sexo e do tempo de maturação sobre a
qualidade da carne ovina. Ciência e Tecnologia de Alimentos, v.24, n.3, p.459-467,
2004. Available from: <http://www.scielo.br/pdf/cta/v24n3/21943.pdf>. Accessed:
Jan. 15, 2012. doi: 10.1590/S0101-20612004000300027.
http://www.scielo.br/pdf/cta/v24n3/21943...
also found
effect in pH under ageing effect and related this with the glycolysis and accumulation
of lactic acid after animal slaughter. There was an interaction (P<0.05) between
saltbush hay level and ageing for lightness value (L*) (Table 2). Meat from the lambs fed with 60% saltbush
hay had a higher lightness (P<0.05) when the shoulders were aged for 14 days than
shoulders aged for 7 days. Differences in the lightness values were not detected for the
other levels of saltbush hay. Shoulders aged for 14 days from lambs fed with 60%
saltbush hay showed the lightness values higher than 30 and 50% saltbush hay but were
similar to the 40% saltbush hay. PEARCE et al.
(2005)PEARCE, K.L. et al. Plasma and tissue α-tocopherol concentrations and
meat colour stability in sheep grazing saltbush (Atriplex spp.). Australian Journal
of Agricultural Research, v.56, p.663-672, 2005. Available from:
<http://www.publish.csiro.au/paper/AR05001.htm>. Accessed: Mar. 15, 2012. doi:
10.1071/AR05001.
http://www.publish.csiro.au/paper/AR0500...
found increase from 2.4 to 6.3mg kg-1 LL muscle
alpha-tocopherol among lambs fed with control and saltbush diet, respectively. The
*L values is a positive effect and the plausible explanation is due
to the saltbush increase in the vitamin E levels on muscle, which improve the color
stability (PEARCE et al., 2010PEARCE, K.L. et al. The role of saltbush-based pasture systems for the
production of high quality sheep and goat meat. Small Ruminant Research, v.91,
p.29-38, 2010. Available from:
<http://www.sciencedirect.com/science/article/pii/S0921448810000258#>.
Accessed: Feb. 20, 2014. doi:10.1016/j.smallrumres.2009.10.018.
http://www.sciencedirect.com/science/art...
).The lightness is
an objective analysis that may be used to predict final meat quality when evaluated
together with other parameters. BONAGURIO et al.
(2003)BONAGURIO, S. et al. Qualidade da carne de cordeiros Santa Ines puros e
mesticos com Texel abatidos com diferentes pesos. Revista Brasileira de Zootecnia,
v.32, n.6, p.1981-1991, 2003. Available from:
<http://www.scielo.br/pdf/rbz/v32n6s2/20971.pdf>. Accessed: Dec. 08, 2011. doi:
10.1590/S1516-35982003000800023.
http://www.scielo.br/pdf/rbz/v32n6s2/209...
reported that meat lightness decreases when the slaughter weight of
sheep increases. Similar to this study, the lightest animals were from feeding with 60%
saltbush hay and had the highest lightness values when the meat was aged for 14 days.
MORENO et al. (2010)MORENO, G.M.B. et al. Desempenho de cordeiros Santa Inês alimentados com
níveis de feno de erva-sal e concentrado. In: Reunião Anual da Sociedade Brasileira
de Zootecnia, 47., 2010, Salvador, BA. Anais... Salvador: Sociedade Brasileira de
Zootecnia, 2010. (CD-ROM). showed that the effect of
including saltbush hay in the slaughter weight decreased when the saltbush hay level was
increased in diet of the animals in the present study (36.31±3.41, 34.88±5.07,
32.75±4.24 and 31.06±4.40kg of the slaughter weight for 30, 40, 50 and 60% saltbush hay,
respectively). ZEOLA et al. (2007)ZEOLA, N.M.B. et al. Capacidade de retenção de agua e maciez da carne de
cordeiro maturada e injetada com cloreto de calcio. Arquivo Brasileiro de Medicina
Veterinária e Zootecnia, v.59, n.4, p.1058-1066, 2007. Available from:
<http://www.scielo.br/pdf/abmvz/v59n4/36.pdf>. Accessed: Oct. 16, 2011. doi:
10.1590/S0102-09352007000400036.
http://www.scielo.br/pdf/abmvz/v59n4/36....
reported that
the ageing influences (P<0.05) the lightness and redness values but does not affect
yellowness values.
Shoulders aged for 14 days had higher (P<0.05) redness values (a*)
than those aged for 7 days and unaged. The average redness values (a*)
for the 40 and 50% saltbush hay samples differed (P<0.05) from the 30% saltbush
samples but were similar to the redness values of the 60% saltbush samples. The higher
redness level in meat is considered a positive effect for the consumers. The yellowness
values (b*) did not differ (P>0.05) among the saltbush hay levels
but was different among ageing time. Shoulders aged for 14 days had higher (6.64)
yellowness value (b*) than those aged for 7 days (5.22) and unaged
(5.08). PEARCE et al. (2005)PEARCE, K.L. et al. Plasma and tissue α-tocopherol concentrations and
meat colour stability in sheep grazing saltbush (Atriplex spp.). Australian Journal
of Agricultural Research, v.56, p.663-672, 2005. Available from:
<http://www.publish.csiro.au/paper/AR05001.htm>. Accessed: Mar. 15, 2012. doi:
10.1071/AR05001.
http://www.publish.csiro.au/paper/AR0500...
identified a
behavior that was similar to these parameters when they evaluated meat from lambs fed
with saltbush pasture. In addition, these authors concluded that consumers prefer redder
meat and that increased yellowness (b*) of meat may reduce its
acceptance by consumers. Including saltbush hay improved meat color by making it redder
without altering the yellowness, which suggests that saltbush hay is a good option for
producing meat that meets consumer acceptance standards.
There was no significant interaction (P>0.05) among ageing and saltbush hay levels
for shear force (SF), cooking loss (CL) and water holding capacity (WHC) of the lamb
shoulder (Table 3). These variables were not
influenced (P>0.05) by saltbush hay level or ageing time, with average values of
3.06kgf cm-2, 37.28% and 76.71% for SF, CL and WHC, respectively. GONÇALVES et al. (2004)GONÇALVES, L.A.G. et al. Efeitos do sexo e do tempo de maturação sobre a
qualidade da carne ovina. Ciência e Tecnologia de Alimentos, v.24, n.3, p.459-467,
2004. Available from: <http://www.scielo.br/pdf/cta/v24n3/21943.pdf>. Accessed:
Jan. 15, 2012. doi: 10.1590/S0101-20612004000300027.
http://www.scielo.br/pdf/cta/v24n3/21943...
also did not find a
significant difference in CL and SF for 1, 3, 7 and 14 days of ageing at 2ºC for the
lamb longissimus muscle, and they explained that the decrease in SF may not
have been observed because the SF results on the first day post mortem
were already sufficiently low (3.78kgf). The results reported by GONÇALVES et al. (2004)GONÇALVES, L.A.G. et al. Efeitos do sexo e do tempo de maturação sobre a
qualidade da carne ovina. Ciência e Tecnologia de Alimentos, v.24, n.3, p.459-467,
2004. Available from: <http://www.scielo.br/pdf/cta/v24n3/21943.pdf>. Accessed:
Jan. 15, 2012. doi: 10.1590/S0101-20612004000300027.
http://www.scielo.br/pdf/cta/v24n3/21943...
were consistent with the results identified
in this study which the average values for SF were 3.44, 3.11 and 2.64kgf
cm-2 for 0, 7 and 14 days of ageing, respectively. WHEELER & KOOHMARAIE (1999)WHEELER. T.L.; KOOHMARAIE, M. The extent of proteolysis is independent
of sarcomere length in lamb longissimus and psoas major. Journal of Animal Science,
v.77, p.2444-2451, 1999. Available from:
<http://www.journalofanimalscience.org/content/77/9/2444.full.pdf>. Accessed:
Mar. 22, 2014.
http://www.journalofanimalscience.org/co...
found slightly lower reduction in
shear force from 1 to 10d and assign this due to the fact that the control psoas major
muscle was already so tender that there was little room for improvement.
ZEOLA et al. (2007)ZEOLA, N.M.B. et al. Capacidade de retenção de agua e maciez da carne de
cordeiro maturada e injetada com cloreto de calcio. Arquivo Brasileiro de Medicina
Veterinária e Zootecnia, v.59, n.4, p.1058-1066, 2007. Available from:
<http://www.scielo.br/pdf/abmvz/v59n4/36.pdf>. Accessed: Oct. 16, 2011. doi:
10.1590/S0102-09352007000400036.
http://www.scielo.br/pdf/abmvz/v59n4/36....
analyzed the effects of
different ageing times and calcium chloride injection on the color, WHC and tenderness
of lamb biceps femoris, longissimus and triceps
brachii muscles. They found that ageing affects the reduction of WHC of the
triceps brachii muscles but not of the biceps femoris and
longissimus muscles, even though the SF values of the biceps
femoris and longissimus muscles are affected by ageing. Although
in this study there was not effect of the saltbush in WHC, PEARCE et al. (2010)PEARCE, K.L. et al. The role of saltbush-based pasture systems for the
production of high quality sheep and goat meat. Small Ruminant Research, v.91,
p.29-38, 2010. Available from:
<http://www.sciencedirect.com/science/article/pii/S0921448810000258#>.
Accessed: Feb. 20, 2014. doi:10.1016/j.smallrumres.2009.10.018.
http://www.sciencedirect.com/science/art...
affirm that the high level of salt in saltbush
may be responsible for the increase in hydration status observed in sheep fed with
saltbush and high vitamin E concentrations in muscle may aid in the retention of water,
but further research are necessary to explain this effect.
Table 4 shows the regression equation for the effects of 30, 40, 50 and 60% saltbush hay in lamb diets. Shoulders unaged had a linear (P<0.05) increase in the results for lightness (L*) and yellowness (b*), with slopes of 0.0530 and 0.0356, respectively. A quadratic behavior was observed for pH, with a minimum value at 43.9% saltbush hay in unaged meat. Shoulders aged for 7 days had a quadratic behavior for redness (a*), with a maximum at 47.95% of saltbush hay. A cubic effect (P<0.05) was observed for the pH, tenderness and overall quality of the product, with estimated maximums at 36, 55 and 54% saltbush hay, respectively, and minimums at 51, 39 and 38% saltbush hay, respectively. Shoulders aged for 14 days had a positive linear effect (P=0.0063) on lightness (L*), with a slope of 0.0744.
CONCLUSION:
There were no detrimental effects of increasing saltbush hay until 60% level in the diet of lambs, and there was improvement in the lightness with 60% saltbush hay level in meat aged for 14 days.
ACKNOWLEDGEMENTS
This work was financially supported by 'Fundação de Amparo à Pesquisa do Estado de São Paulo' (FAPESP) and 'Banco Nacional do Desenvolvimento' (BNDES).
Referências
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» https://doi.org/10.1016/S0921-4488(96)00932-7» http://www.sciencedirect.com/science/article/pii/S0921448896009327 - ABU-ZANAT, M.M.W. Voluntary intake and digestibility of saltbush by sheep. Asian-Australian Journal Animal Science, v.18, n.2, p.214-220, 2005. Available from: <http://www2.ju.edu.jo/sites/Academic/mahfouz/Lists/Published%20Research/Attachments/4/Voluntary%20Intake%20and%20Digestibility2005.pdf>. Accessed: Mar. 22, 2014.
» http://www2.ju.edu.jo/sites/Academic/mahfouz/Lists/Published%20Research/Attachments/4/Voluntary%20Intake%20and%20Digestibility2005.pdf - BARBOSA, J.C.; MALDONADO JR, W. AgroEstat. Sistema para análises estatísticas de ensaios agronômicos. Versao 1.0. Universidade Estadual Paulista "Júlio de Mesquita Filho" - Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, Brasil, 2010.
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» https://doi.org/10.1016/j.smallrumres.2009.10.017» http://www.sciencedirect.com/science/article/pii/S0921448810000246 - BONAGURIO, S. et al. Qualidade da carne de cordeiros Santa Ines puros e mesticos com Texel abatidos com diferentes pesos. Revista Brasileira de Zootecnia, v.32, n.6, p.1981-1991, 2003. Available from: <http://www.scielo.br/pdf/rbz/v32n6s2/20971.pdf>. Accessed: Dec. 08, 2011. doi: 10.1590/S1516-35982003000800023.
» https://doi.org/10.1590/S1516-35982003000800023» http://www.scielo.br/pdf/rbz/v32n6s2/20971.pdf - GONÇALVES, L.A.G. et al. Efeitos do sexo e do tempo de maturação sobre a qualidade da carne ovina. Ciência e Tecnologia de Alimentos, v.24, n.3, p.459-467, 2004. Available from: <http://www.scielo.br/pdf/cta/v24n3/21943.pdf>. Accessed: Jan. 15, 2012. doi: 10.1590/S0101-20612004000300027.
» https://doi.org/10.1590/S0101-20612004000300027» http://www.scielo.br/pdf/cta/v24n3/21943.pdf - HAMM, R. Biochemistry of meat hydration. Advanceds in Food Research, v.10, p.335-443, 1961. Available from: <http://www.sciencedirect.com/science/article/pii/S006526280860141X>. Accessed: Jan. 10, 2012. doi: 10.1016/S0065-2628 (08) 60141-X.
» https://doi.org/10.1016/S0065-2628 (08) 60141-X» http://www.sciencedirect.com/science/article/pii/S006526280860141X - MARTÍNEZ-CEREZO, S. et al. Breed, slaughter weight and ageing time effects on consumer appraisal of three muscles of lamb. Meat Science, v.69, p.795-805, 2005. Available from: <http://www.sciencedirect.com/science/article/pii/S0309174004002955>. Accessed: Feb. 05, 2012. doi: 10.1016/j.meatsci.2004.11.013.
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COMITÊ DE ÉTICA E BIOSSEGURANÇA Protocolo da aprovação do trabalho pelo Comitê de Ética é: 008403-08.
Publication Dates
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Publication in this collection
Dec 2014
History
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Received
01 July 2013 -
Accepted
17 Apr 2014