Serological survey of Neospora caninum in small ruminants from Pernambuco State, Brazil

Inquérito sorológico de Neospora caninum em pequenos ruminantes do estado de Pernambuco, Brasil

RESEARCH NOTE

Serological survey of Neospora caninum in small ruminants from Pernambuco State, Brazil

Inquérito sorológico de Neospora caninum em pequenos ruminantes do estado de Pernambuco, Brasil

Antonio Amélia Santos Mucalane Tembue^I; Rafael Antonio de Nascimento Ramos^I; Thais Rabelo de Sousa^II; Alessandra Ribeiro Albuquerque^I; Alvimar José da Costa^II; Isabelle Maria Jaqueline Meunier^I; Maria Aparecida da Gloria Faustino^I; Leucio Câmara Alves^I

^ILaboratório de Doenças Parasitárias dos Animais Domésticos, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco - UFRPE

^IICentro de Pesquisa em Sanidade Animal - CPPAR, Universidade Estadual Paulista - UNESP

^{Corresponding author}

ABSTRACT

Neospora caninum infection is an important cause of bovine abortion as well as neonatal mortality in goats and sheep. A serological survey for antibodies against N. caninum in goats and sheep was carried out in the municipality of Ibimirim, PE, Northeastern Brazil. The imunnofluoresce antibody test showed that 26.6% (85/319) of the goats and 64.2% (52/81) of the sheep were positive. Serologic reactivity was associated with age in goats (p < 0.01) and sheep (p > 0.05), with increasing rates in older animals. These results indicate exposure to N. caninum among small ruminants in the study area.

Keywords: Coccidiosis, small ruminants, serology.

RESUMO

A infecção por Neospora caninum é uma importante causa de abortos em bovinos, assim como mortalidade neonatal em caprinos e ovinos. Uma pesquisa sorológica de anticorpos anti-N. caninum foi realizada no município de Ibimirim (PE, Brasil). Os resultados ao teste de imunofluorescência revelaram que 26,6% (85/319) dos caprinos e 64,2% (52/81) dos ovinos foram positivos. A reatividade à sorologia foi associada com a idade nos caprinos (p < 0,01) e ovinos (p > 0,05), com maior ocorrência nos animais mais velhos. Estes resultados indicam exposição a N. caninum entre pequenos ruminantes na área estudada.

Palavras-chave: Cocciodiose, pequenos ruminantes, sorologia.

Neosporosis is an important parasitic disease caused by Neospora caninum (DUBEY, 2003). Intermediate hosts of this agent in its life cycle are goats, sheep and cattle, and definitive hosts are canids (GONDIM et al., 2004).

It is a common and an important cause of abortions, endemic and epidemic, in cattle raise (ANDERSON et al., 1995), but its occurrence in goats and sheep should also be considered, and the parasite has been described associated with births of weak and premature animals (CORBELLINI et al., 2001).

Epidemiological studies carried out in different geographic regions indicate varying prevalence rates in different species, for which the main means of transmission is vertical (DUBEY, 2003). Serological results in goats and sheep from Brazil indicate prevalence rates ranging from 9.2 to 29% (FIGLIUOLO et al., 2004a; ROMANELLI et al., 2007).

The aim of the present study was to determine the prevalence of anti-N. caninum antibodies in sheep and goats in the municipality of Ibimirim in the Moxotó Semi-Arid region in the State of Pernambuco, Northeastern Brazil.

Blood samples collected from 319 goats and 81 sheep from 23 properties were studied. Owners were interviewed to obtain information regarding sex, age, management, type of system used and presence of dogs. Age was estimated through the dental arcade when unknown by the owners. Ten percent of the females from each property were studied, including those animals under one year of age, using non-probabilistic convenience methodology (COSTA NETO, 1977). All properties raised goats and sheep.

The indirect fluorescence antibody test (IFAT) was performed to detect anti-N. caninum antibodies based on the methods described by Conrad et al. (1993) and Barr et al. (1995) using conjugate anti-goat IgG (Conjugate anti-IgG goat Sigma, USA), and anti-sheep IgG (Conjugate anti-IgG-sheep Sigma, USA), with a cut-off value of 1:50 (DUBEY; LINDSAY, 1996). A reaction was considered positive when tachyzoites showed total peripheral fluorescence (PARÉ et al., 1995).

The data analysis employed the chi-square (χ²) test, with the level of significance set at 5% (p < 0.05) to assess the association with age, sex and presence of anti-N. caninum antibodies.

The serological results revealed that 26.6% (85/319) of the goats were positive for anti-N. caninum antibodies. This rate is higher than 6.34% found by Figliuolo et al. (2004b) and 17.44% found by Stachissini (2005), both in the State of São Paulo, Southeastern Brazil. These differences may be explained by the use of different serological tests, survey periods, sample sizes and cut-off values. Climatic factors may also affect the abundance of viable parasitic stages in the environment for definitive and intermediate hosts and influence the overall prevalence (FARIA et al., 2007).

With respect to sheep 64.2% (52/81) were positive for anti-N. caninum antibodies. This rate is also higher than 9.2% found by Figliuolo et al. (2004a).

According to Dubey (2003), very few studies on the prevalence of infection in small ruminants have been carried out but the reason for a higher prevalence of sheep infection may be correlated to greater susceptibility of the species (DUBEY; LINDSAY, 1996).

It is important to mention that seropositive animals did not present clinical signs suggesting neosporosis. According to Dubey and Lindsay (1996), goats inoculated with N. caninum develop infection, but do not develop clinical disease and only protective antibodies are observed.

Despite the higher positive results seen among females, no statistically significant difference was found regarding sex, with 10 males and 127 females positive to N. caninum.

Regarding age, a statistically significant difference (χ² = 12.82) was found (p < 0.01) among goats, with older presenting higher rates than younger animals (Table 1). The same pattern was found in sheep (χ² = 4.83), but with no statistically significant difference (p > 0.05) (Table 1).

In the farms visited, 81.3% of them had dogs, which facilitates contamination of crops by oocysts eliminated through feces. However information regarding the serological status of the dogs was not available.

The results indicate that N. caninum is present in small ruminants of the study area, despite no clinical signs of the disease.

Received September 10, 2010

Accepted December 21, 2010

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Publication Dates

• Publication in this collection
  14 Oct 2011
• Date of issue
  Sept 2011

History

• Accepted
  21 Dec 2010
• Received
  10 Sept 2010