Acessibilidade / Reportar erro

Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum

Caldo de cana induz pectina liase e poligalacturonase em Penicillium griseoroseum

The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low concentrations, sugar-cane juice was capable of inducing pectin lyase and polygalacturonase with no cellulase activity in P. griseoroseum.

pectin lyase; polygalacturonase; pectinases; Penicillium griseoroseum; sugar-cane juice


Sociedade Brasileira de Microbiologia Av. Prof. Lineu Prestes, 1374, Cidade Universitária, 05508-900 São Paulo SP - Brazil, Tel. / Fax: (55 11) 813.9647 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br